(Linear) (Six-base) MAPSORT of: KIEE5008.seq Check: 7483 from: 1 to: 1005 KIEE5008.orf With 182 enzymes: SgfI * December 5, 2014 12:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 747 1005 Size: 747 258 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 281 526 678 1005 Size: 281 245 152 327 Fragments arranged by size: 327 281 245 152 AfeI AGC'GCT Cuts at: 0 421 1005 Size: 421 584 AhdI GACnn_n'nnGTC Cuts at: 0 192 1005 Size: 192 813 AleI CACnn'nnGTG Cuts at: 0 613 925 1005 Size: 613 312 80 Fragments arranged by size: 613 312 80 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 943 1005 Size: 943 62 AlwNI CAG_nnn'CTG Cuts at: 0 504 537 871 1001 1005 Size: 504 33 334 130 4 Fragments arranged by size: 504 334 130 33 4 ApoI r'AATT_y Cuts at: 0 112 1005 Size: 112 893 BanII G_rGCy'C Cuts at: 0 631 1005 Size: 631 374 BbsI GAAGACnn'nnnn_ Cuts at: 0 413 1005 Size: 413 592 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 427 461 1005 Size: 427 34 544 Fragments arranged by size: 544 427 34 BglII A'GATC_T Cuts at: 0 57 275 1005 Size: 57 218 730 Fragments arranged by size: 730 218 57 Bme1580I G_kGCm'C Cuts at: 0 798 1005 Size: 798 207 BmgBI CAC'GTC Cuts at: 0 700 1005 Size: 700 305 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 370 820 1005 Size: 370 450 185 Fragments arranged by size: 450 370 185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 654 1005 Size: 654 351 BsaI GGTCTCn'nnnn_ Cuts at: 0 179 767 1005 Size: 179 588 238 Fragments arranged by size: 588 238 179 BsaWI w'CCGG_w Cuts at: 0 10 1005 Size: 10 995 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 46 1005 Size: 46 959 BseYI C'CCAG_C Cuts at: 0 580 664 864 1005 Size: 580 84 200 141 Fragments arranged by size: 580 200 141 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 565 637 1005 Size: 271 294 72 368 Fragments arranged by size: 368 294 271 72 BsmBI CGTCTCn'nnnn_ Cuts at: 0 145 900 1005 Size: 145 755 105 Fragments arranged by size: 755 145 105 Bsp1286I G_dGCh'C Cuts at: 0 631 798 1005 Size: 631 167 207 Fragments arranged by size: 631 207 167 BspEI T'CCGG_A Cuts at: 0 10 1005 Size: 10 995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 747 1005 Size: 747 258 BsrDI GCAATG_nn' Cuts at: 0 647 1005 Size: 647 358 BsrFI r'CCGG_y Cuts at: 0 269 1005 Size: 269 736 BssHII G'CGCG_C Cuts at: 0 384 1005 Size: 384 621 BssSI C'ACGA_G Cuts at: 0 829 954 1005 Size: 829 125 51 Fragments arranged by size: 829 125 51 BstXI CCAn_nnnn'nTGG Cuts at: 0 588 1005 Size: 588 417 BstYI r'GATC_y Cuts at: 0 57 275 1005 Size: 57 218 730 Fragments arranged by size: 730 218 57 BtgI C'CryG_G Cuts at: 0 623 1005 Size: 623 382 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 79 1005 Size: 79 926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 630 665 1005 Size: 630 35 340 Fragments arranged by size: 630 340 35 EaeI y'GGCC_r Cuts at: 0 539 910 1005 Size: 539 371 95 Fragments arranged by size: 539 371 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 891 1005 Size: 891 114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 281 370 526 678 820 1005 Size: 281 89 156 152 142 185 Fragments arranged by size: 281 185 156 152 142 89 EcoO109I rG'GnC_Cy Cuts at: 0 569 661 725 1005 Size: 569 92 64 280 Fragments arranged by size: 569 280 92 64 EcoRI G'AATT_C Cuts at: 0 112 1005 Size: 112 893 EcoRV GAT'ATC Cuts at: 0 752 1005 Size: 752 253 HaeII r_GCGC'y Cuts at: 0 423 1005 Size: 423 582 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 178 210 384 416 1005 Size: 178 32 174 32 589 Fragments arranged by size: 589 178 174 32 32 MfeI C'AATT_G Cuts at: 0 325 1005 Size: 325 680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 929 1005 Size: 929 76 MslI CAynn'nnrTG Cuts at: 0 394 613 925 1005 Size: 394 219 312 80 Fragments arranged by size: 394 312 219 80 MspA1I CmG'CkG Cuts at: 0 159 613 965 1005 Size: 159 454 352 40 Fragments arranged by size: 454 352 159 40 PflMI CCAn_nnn'nTGG Cuts at: 0 871 1005 Size: 871 134 PfoI T'CCnGG_A Cuts at: 0 116 452 485 1005 Size: 116 336 33 520 Fragments arranged by size: 520 336 116 33 PpuMI rG'GwC_Cy Cuts at: 0 569 661 725 1005 Size: 569 92 64 280 Fragments arranged by size: 569 280 92 64 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 817 849 1005 Size: 817 32 156 Fragments arranged by size: 817 156 32 PvuII CAG'CTG Cuts at: 0 159 613 1005 Size: 159 454 392 Fragments arranged by size: 454 392 159 SanDI GG'GwC_CC Cuts at: 0 725 1005 Size: 725 280 SexAI A'CCwGG_T Cuts at: 0 985 1005 Size: 985 20 SmlI C'TyrA_G Cuts at: 0 669 1005 Size: 669 336 StyI C'CwwG_G Cuts at: 0 284 1005 Size: 284 721 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 925 1005 Size: 925 80 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 930 1005 Size: 930 75 XmnI GAAnn'nnTTC Cuts at: 0 494 1005 Size: 494 511 Enzymes that do cut: AarI AcuI AfeI AhdI AleI AlfI AlwNI ApoI BanII BbsI BcgI BglII Bme1580I BmgBI BpmI BpuEI BsaI BsaWI BseRI BseYI BsgI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BssHII BssSI BstXI BstYI BtgI BtgZI CspCI EaeI EciI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I MfeI MmeI MslI MspA1I PflMI PfoI PpuMI PsrI PvuII SanDI SexAI SmlI StyI TaqII XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglI BlpI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaXI BsiEI BsiHKAI BsiWI BsmI BspHI BsrBI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI EagI EarI EcoICRI EcoNI FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI