Restriction map for
pFN21AE1399
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# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AgeI AhdI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BbvCI BciVI BclI BlnI BsiWI
BspLU11I BssHII Bst1107I BstBI BstZ17I
DraI EagI Eam1105I EclXI Eco52I
FbaI FseI FspI KpnI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PinAI PmeI
PshBI PvuI SalI ScaI SfuI
SnaBI SpeI SphI SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 4819 GACGTC GACGT^C
AccIII 2294 TCCGGA T^CCGGA
AclI 4864 AACGTT AA^CGTT
AflII 2140 CTTAAG C^TTAAG
AflIII 3911 ACACGT A^C[GA][CT]GT
Alw44I 3946 GTGCAC G^TGCAC
ApaLI 3946 GTGCAC G^TGCAC
Asp700I 1800 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 3478 GGCGCC GGCGC^C
BbrPI 928 CACGTG CAC^GTG
BfrI 2140 CTTAAG C^TTAAG
BglII 4079 AGATCT A^GATCT
BsaBI 3897 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2294 TCCGGA T^CCGGA
BsiEI 3261 CGACCG CG[GA][CT]^CG
Bsp1407I 265 TGTACA T^GTACA
BspEI 2294 TCCGGA T^CCGGA
BspHI 4991 TCATGA T^CATGA
BsrGI 265 TGTACA T^GTACA
CpoI 2291 CGGTCCG CG^G[AT]CCG
DrdI 1294 GACTGGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 4264 GATATC GAT^ATC
HpaI 2062 GTTAAC GTT^AAC
KasI 3478 GGCGCC G^GCGCC
MamI 3897 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2294 TCCGGA T^CCGGA
NarI 3478 GGCGCC GG^CGCC
NcoI 4139 CCATGG C^CATGG
PmaCI 928 CACGTG CAC^GTG
PmlI 928 CACGTG CAC^GTG
PshAI 1875 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4864 AACGTT AA^CGTT
RcaI 4991 TCATGA T^CATGA
RsrII 2291 CGGTCCG CG^G[AT]CCG
SfiI 3711 GGCCCCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3478 GGCGCC GGC^GCC
SgrAI 1207 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 265 TGTACA T^GTACA
XmnI 1800 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 2746 GTCTAC GT^[AC][GT]AC
2998 GTCTAC
AocI 3816 CCTGAGG CC^T[ACGT]AGG
5102 CCTCAGG
Aor51HI 1285 AGCGCT AGC^GCT
3409 AGCGCT
AspI 1651 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4450 GACGCCGTC
BamHI 257 GGATCC G^GATCC
561 GGATCC
BfaI 3488 CTAG C^TAG
4541 CTAG
BlpI 613 GCTCAGC GC^T[ACGT]AGC
3412 GCTGAGC
Bpu1102I 613 GCTCAGC GC^T[ACGT]AGC
3412 GCTGAGC
BsaAI 928 CACGTG [CT]AC^GT[GA]
1399 TACGTG
BsmBI 3829 GAGACG CGTCTC(1/5)
4513 GAGACG
BspDI 5434 ATCGAT AT^CGAT
5476 ATCGAT
Bsu36I 3816 CCTGAGG CC^T[ACGT]AGG
5102 CCTCAGG
CelII 613 GCTCAGC GC^T[ACGT]AGC
3412 GCTGAGC
ClaI 5434 ATCGAT AT^CGAT
5476 ATCGAT
Eco47III 1285 AGCGCT AGC^GCT
3409 AGCGCT
Eco81I 3816 CCTGAGG CC^T[ACGT]AGG
5102 CCTCAGG
EcoNI 345 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5457 CCTGCAGAAGG
EcoT22I 210 ATGCAT ATGCA^T
498 ATGCAT
HincII 2062 GTTAAC GT[CT]^[GA]AC
4621 GTTGAC
HindII 2062 GTTAAC GT[CT]^[GA]AC
4621 GTTGAC
HindIII 1801 AAGCTT A^AGCTT
4443 AAGCTT
KspI 1644 CCGCGG CCGC^GG
1704 CCGCGG
MaeI 3488 CTAG C^TAG
4541 CTAG
NsiI 210 ATGCAT ATGCA^T
498 ATGCAT
NspI 1485 GCATGT [GA]CATG^[CT]
3890 ACATGC
PflFI 1651 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4450 GACGCCGTC
SacI 662 GAGCTC GAGCT^C
2272 GAGCTC
SacII 1644 CCGCGG CCGC^GG
1704 CCGCGG
SapI 2584 GAAGAGC GCTCTTC(1/4)
1587 GCTCTTC
SbfI 914 CCTGCAGG CCTGCA^GG
3981 CCTGCAGG
SexAI 2132 ACCTGGT A^CC[AT]GGT
2806 ACCAGGT
Sse8387I 914 CCTGCAGG CCTGCA^GG
3981 CCTGCAGG
Tth111I 1651 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4450 GACGCCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BcnI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PaeR7I PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI