(Circular) (Six-base) MAPSORT of: pFN21AE1399.seq Check: 9835 from: 1 to: 10252 pFN21AE1399 10252 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2961 4093 6599 2961 Size: 1132 2506 6614 Fragments arranged by size: 6614 2506 1132 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6828 8062 278 Size: 53 83 186 936 5292 1234 2468 Fragments arranged by size: 5292 2468 1234 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4752 5004 7595 1937 Size: 2815 252 2591 4594 Fragments arranged by size: 4594 2815 2591 252 Acc65I G'GTAC_C Cuts at: 7573 7573 Size: 10252 AclI AA'CG_TT Cuts at: 1563 6870 8379 8752 1563 Size: 5307 1509 373 3063 Fragments arranged by size: 5307 3063 1509 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2516 2765 4640 4843 4914 8315 9699 9967 Size: 884 249 1875 203 71 3401 1384 268 Cuts at: 9967 1632 Size: 1917 Fragments arranged by size: 3401 1917 1875 1384 884 268 249 203 71 AfeI AGC'GCT Cuts at: 3292 5416 3292 Size: 2124 8128 AflII C'TTAA_G Cuts at: 820 1017 4145 820 Size: 197 3128 6927 Fragments arranged by size: 6927 3128 197 AflIII A'CryG_T Cuts at: 1203 5916 9151 10128 1203 Size: 4713 3235 977 1327 Fragments arranged by size: 4713 3235 1327 977 AgeI A'CCGG_T Cuts at: 9083 9083 Size: 10252 AhdI GACnn_n'nnGTC Cuts at: 8981 8981 Size: 10252 AleI CACnn'nnGTG Cuts at: 1166 2174 3651 6110 1166 Size: 1008 1477 2459 5308 Fragments arranged by size: 5308 2459 1477 1008 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10252 AlwNI CAG_nnn'CTG Cuts at: 2944 3352 3861 3997 4603 6293 7543 7995 9567 Size: 408 509 136 606 1690 1250 452 1572 Cuts at: 9567 2944 Size: 3629 Fragments arranged by size: 3629 1690 1572 1250 606 509 452 408 136 ApaI G_GGCC'C Cuts at: 3417 3558 5550 3417 Size: 141 1992 8119 Fragments arranged by size: 8119 1992 141 ApaLI G'TGCA_C Cuts at: 5951 8309 9465 5951 Size: 2358 1156 6738 Fragments arranged by size: 6738 2358 1156 ApoI r'AATT_y Cuts at: 1493 3959 4783 5524 6251 7561 7787 7939 10048 Size: 2466 824 741 727 1310 226 152 2109 Cuts at: 10048 1493 Size: 1697 Fragments arranged by size: 2466 2109 1697 1310 824 741 727 226 152 AseI AT'TA_AT Cuts at: 160 1032 8806 160 Size: 872 7774 1606 Fragments arranged by size: 7774 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10252 AvaI C'yCGr_G Cuts at: 1870 1943 2721 2992 3246 3374 3410 3550 3584 Size: 73 778 271 254 128 36 140 34 Cuts at: 3584 4319 4524 5072 5384 5549 5597 5811 6098 Size: 735 205 548 312 165 48 214 287 Cuts at: 6098 6515 7497 7577 1870 Size: 417 982 80 4545 Fragments arranged by size: 4545 982 778 735 548 417 312 287 271 254 214 205 165 140 128 80 73 48 36 34 BamHI G'GATC_C Cuts at: 2262 2566 7582 7949 2262 Size: 304 5016 367 4565 Fragments arranged by size: 5016 4565 367 304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 3515 4222 5483 6046 Size: 325 217 627 354 1374 707 1261 563 Cuts at: 6046 7052 7573 9028 618 Size: 1006 521 1455 1842 Fragments arranged by size: 1842 1455 1374 1261 1006 707 627 563 521 354 325 217 BanII G_rGCy'C Cuts at: 1391 2671 3417 3558 3833 4281 4433 4499 5463 Size: 1280 746 141 275 448 152 66 964 Cuts at: 5463 5550 6105 6143 7571 1391 Size: 87 555 38 1428 4072 Fragments arranged by size: 4072 1428 1280 964 746 555 448 275 152 141 87 66 38 BbeI G_GCGC'C Cuts at: 5487 5487 Size: 10252 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2492 2753 3395 3884 4902 5084 6555 Size: 971 593 261 642 489 1018 182 1471 Cuts at: 6555 928 Size: 4625 Fragments arranged by size: 4625 1471 1018 971 642 593 489 261 182 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5149 5182 5183 5216 5477 Size: 34 50 34 3128 33 1 33 261 Cuts at: 5477 5511 6109 6143 8443 8477 1903 Size: 34 598 34 2300 34 3678 Fragments arranged by size: 3678 3128 2300 598 261 50 34 34 34 34 34 33 33 1 BciVI GTATCCnnnnn_n' Cuts at: 8144 9354 8144 Size: 1210 9042 BclI T'GATC_A Cuts at: 1548 1548 Size: 10252 BfrBI ATG'CAT Cuts at: 2217 2505 2217 Size: 288 9964 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3487 3589 5026 Size: 107 122 71 631 605 1815 102 1437 Cuts at: 5026 5723 8863 136 Size: 697 3140 1525 Fragments arranged by size: 3140 1815 1525 1437 697 631 605 122 107 102 71 BglII A'GATC_T Cuts at: 6084 10247 6084 Size: 4163 6089 BlpI GC'TnA_GC Cuts at: 2619 5418 7669 2619 Size: 2799 2251 5202 Fragments arranged by size: 5202 2799 2251 Bme1580I G_kGCm'C Cuts at: 1790 2934 2983 3183 3411 3417 3558 3585 4225 Size: 1144 49 200 228 6 141 27 640 Cuts at: 4225 4689 5550 5571 5955 6117 6772 7126 7509 Size: 464 861 21 384 162 655 354 383 Cuts at: 7509 8313 9469 1790 Size: 804 1156 2573 Fragments arranged by size: 2573 1156 1144 861 804 655 640 464 384 383 354 228 200 162 141 49 27 21 6 BmgBI CAC'GTC Cuts at: 1318 5082 5532 5919 7121 1318 Size: 3764 450 387 1202 4449 Fragments arranged by size: 4449 3764 1202 450 387 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3310 6632 7983 Size: 416 53 83 399 24 1893 3322 1351 Cuts at: 7983 8941 442 Size: 958 1753 Fragments arranged by size: 3322 1893 1753 1351 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10252 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2432 2966 4019 4292 6346 6779 8912 1239 Size: 1193 534 1053 273 2054 433 2133 2579 Fragments arranged by size: 2579 2133 2054 1193 1053 534 433 273 Bpu10I CC'TnA_GC Cuts at: 2611 6648 2611 Size: 4037 6215 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 8377 9242 9540 8377 Size: 865 298 9089 Fragments arranged by size: 9089 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2090 2582 2591 5846 5862 7144 7157 Size: 617 591 492 9 3255 16 1282 13 Cuts at: 7157 8915 882 Size: 1758 2219 Fragments arranged by size: 3255 2219 1758 1282 617 591 492 16 13 9 BsaAI yAC'GTr Cuts at: 493 1204 2935 3406 8043 493 Size: 711 1731 471 4637 2702 Fragments arranged by size: 4637 2702 1731 711 471 BsaBI GATnn'nnATC Cuts at: 5906 10246 5906 Size: 4340 5912 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4478 5484 5964 6456 Size: 53 83 186 936 2945 1006 480 492 Cuts at: 6456 6825 8059 8441 9076 275 Size: 369 1234 382 635 1451 Fragments arranged by size: 2945 1451 1234 1006 936 635 492 480 382 369 186 83 53 BsaWI w'CCGG_w Cuts at: 2591 4299 5871 8685 9083 9357 9504 9915 2591 Size: 1708 1572 2814 398 274 147 411 2928 Fragments arranged by size: 2928 2814 1708 1572 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3740 3770 4865 4895 5831 Size: 30 165 30 2337 30 1095 30 936 Cuts at: 5831 5861 6567 6597 7183 7213 1178 Size: 30 706 30 586 30 4217 Fragments arranged by size: 4217 2337 1095 936 706 586 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2372 3761 3887 4289 4337 4599 5402 Size: 982 204 1389 126 402 48 262 803 Cuts at: 5402 6171 6231 6494 1186 Size: 769 60 263 4944 Fragments arranged by size: 4944 1389 982 803 769 402 263 262 204 126 60 48 BseYI C'CCAG_C Cuts at: 3345 3365 3445 3982 4406 5510 5560 5639 5768 Size: 20 80 537 424 1104 50 79 129 Cuts at: 5768 5922 6771 7059 7273 9455 9951 3345 Size: 154 849 288 214 2182 496 3646 Fragments arranged by size: 3646 2182 1104 849 537 496 424 288 214 154 129 80 79 50 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2843 2957 6577 1163 Size: 573 1107 114 3620 4838 Fragments arranged by size: 4838 3620 1107 573 114 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5269 7702 8463 9491 1540 Size: 275 186 3268 2433 761 1028 2301 Fragments arranged by size: 3268 2433 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2671 2952 4281 5087 5500 5955 7004 7025 8313 Size: 281 1329 806 413 455 1049 21 1288 Cuts at: 8313 8398 9469 2671 Size: 85 1071 3454 Fragments arranged by size: 3454 1329 1288 1071 1049 806 455 413 281 85 21 BsmI GAATG_Cn' Cuts at: 2217 2992 4918 7763 7856 2217 Size: 775 1926 2845 93 4613 Fragments arranged by size: 4613 2845 1926 775 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5828 6512 5828 Size: 684 9568 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2671 2934 2952 2983 3183 3411 3417 Size: 399 881 263 18 31 200 228 6 Cuts at: 3417 3558 3585 3833 4225 4281 4433 4499 4689 Size: 141 27 248 392 56 152 66 190 Cuts at: 4689 5087 5463 5500 5550 5571 5955 6105 6117 Size: 398 376 37 50 21 384 150 12 Cuts at: 6117 6143 6772 7004 7025 7126 7509 7571 8313 Size: 26 629 232 21 101 383 62 742 Cuts at: 8313 8398 9469 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 881 742 629 399 398 392 384 383 376 263 248 232 228 200 190 152 150 141 101 85 66 62 56 50 37 31 27 26 21 21 18 12 6 BspEI T'CCGG_A Cuts at: 4299 9915 4299 Size: 5616 4636 BspHI T'CATG_A Cuts at: 6996 8141 9871 6996 Size: 1145 1730 7377 Fragments arranged by size: 7377 1730 1145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2208 2913 2961 4093 6599 7470 7607 844 Size: 1364 705 48 1132 2506 871 137 3489 Fragments arranged by size: 3489 2506 1364 1132 871 705 137 48 BsrBI CCG'CTC Cuts at: 1394 1823 2362 2618 4594 6746 7211 8139 1394 Size: 429 539 256 1976 2152 465 928 3507 Fragments arranged by size: 3507 2152 1976 928 539 465 429 256 BsrDI GCAATG_nn' Cuts at: 65 1248 2335 6233 6688 8747 8921 65 Size: 1183 1087 3898 455 2059 174 1396 Fragments arranged by size: 3898 2059 1396 1183 1087 455 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2320 3213 3423 3973 4413 4916 5591 Size: 110 510 893 210 550 440 503 675 Cuts at: 5591 5780 7080 8896 9083 1700 Size: 189 1300 1816 187 2869 Fragments arranged by size: 2869 1816 1300 893 675 550 510 503 440 210 189 187 110 BsrGI T'GTAC_A Cuts at: 96 2270 96 Size: 2174 8078 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10252 BssSI C'ACGA_G Cuts at: 2903 4280 6704 8312 9324 2903 Size: 1377 2424 1608 1012 3831 Fragments arranged by size: 3831 2424 1608 1377 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3361 4416 4684 794 Size: 2567 1055 268 6362 Fragments arranged by size: 6362 2567 1055 268 BstBI TT'CG_AA Cuts at: 9056 9056 Size: 10252 BstEII G'GTnAC_C Cuts at: 1184 4159 5794 7030 1184 Size: 2975 1635 1236 4406 Fragments arranged by size: 4406 2975 1635 1236 BstXI CCAn_nnnn'nTGG Cuts at: 2551 3235 4171 5323 5974 2551 Size: 684 936 1152 651 6829 Fragments arranged by size: 6829 1152 936 684 651 BstYI r'GATC_y Cuts at: 1972 2262 2566 2907 3636 6084 7255 7582 7949 Size: 290 304 341 729 2448 1171 327 367 Cuts at: 7949 8334 8351 9803 9889 9901 10247 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2448 1977 1452 1171 729 385 367 346 341 327 304 290 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5822 7108 5822 Size: 1286 8966 BtgI C'CryG_G Cuts at: 513 1065 2064 2388 3315 3649 3709 3825 4005 Size: 552 999 324 927 334 60 116 180 Cuts at: 4005 4824 5958 6144 6198 513 Size: 819 1134 186 54 4567 Fragments arranged by size: 4567 1134 999 927 819 552 334 324 186 180 116 60 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2507 3417 5233 5895 487 Size: 683 56 1281 910 1816 662 4844 Fragments arranged by size: 4844 1816 1281 910 683 662 56 BtsI GCAGTG_nn' Cuts at: 2121 5571 6772 7768 8562 8582 2121 Size: 3450 1201 996 794 20 3791 Fragments arranged by size: 3791 3450 1201 996 794 20 ClaI AT'CG_AT Cuts at: 1554 7440 7482 1554 Size: 5886 42 4324 Fragments arranged by size: 5886 4324 42 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2644 2679 7116 7151 529 Size: 35 2080 35 4437 35 3630 Fragments arranged by size: 4437 3630 2080 35 35 35 DraI TTT'AAA Cuts at: 7556 7556 Size: 10252 DraIII CAC_nnn'GTG Cuts at: 2244 2877 5959 2244 Size: 633 3082 6537 Fragments arranged by size: 6537 3082 633 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3305 9259 809 Size: 507 1989 5954 1802 Fragments arranged by size: 5954 1989 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2469 3114 Size: 54 1425 50 275 18 492 147 645 Cuts at: 3114 3237 3318 3712 4093 4827 6783 7377 7699 Size: 123 81 394 381 734 1956 594 322 Cuts at: 7699 8588 10217 8 Size: 889 1629 43 Fragments arranged by size: 1956 1629 1425 889 734 645 594 492 394 381 322 275 147 123 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7699 1537 Size: 275 5887 4090 Fragments arranged by size: 5887 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2088 3599 4584 5988 6144 8182 1353 Size: 735 1511 985 1404 156 2038 3423 Fragments arranged by size: 3423 2038 1511 1404 985 735 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3081 3749 5044 6955 8839 9213 Size: 1064 138 1492 668 1295 1911 1884 374 Cuts at: 9213 9359 387 Size: 146 1280 Fragments arranged by size: 1911 1884 1492 1295 1280 1064 668 374 146 138 EcoICRI GAG'CTC Cuts at: 2669 4279 2669 Size: 1610 8642 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2432 2516 2765 2966 4019 4292 4640 Size: 393 800 84 249 201 1053 273 348 Cuts at: 4640 4843 4914 6346 6779 8315 8912 9699 9967 Size: 203 71 1432 433 1536 597 787 268 Cuts at: 9967 1239 Size: 1524 Fragments arranged by size: 1536 1524 1432 1053 800 787 597 433 393 348 273 268 249 203 201 84 71 EcoNI CCTnn'n_nnAGG Cuts at: 2354 7466 2354 Size: 5112 5140 EcoO109I rG'GnC_Cy Cuts at: 3193 3250 3414 3554 5179 5546 5702 5716 5816 Size: 57 164 140 1625 367 156 14 100 Cuts at: 5816 6804 7104 7474 3193 Size: 988 300 370 5971 Fragments arranged by size: 5971 1625 988 370 367 300 164 156 140 100 57 14 EcoRI G'AATT_C Cuts at: 3959 4783 5524 7561 7939 3959 Size: 824 741 2037 378 6272 Fragments arranged by size: 6272 2037 824 741 378 EcoRV GAT'ATC Cuts at: 6271 6271 Size: 10252 FspI TGC'GCA Cuts at: 8758 8758 Size: 10252 HaeII r_GCGC'y Cuts at: 1678 3294 3708 5418 5487 9399 1678 Size: 1616 414 1710 69 3912 2531 Fragments arranged by size: 3912 2531 1710 1616 414 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3518 3550 4153 4185 4277 Size: 32 135 32 1946 32 603 32 92 Cuts at: 4277 4309 4427 4459 4865 4897 5411 5443 5627 Size: 32 118 32 406 32 514 32 184 Cuts at: 5627 5659 8893 8925 8967 8999 1373 Size: 32 3234 32 42 32 2626 Fragments arranged by size: 3234 2626 1946 603 514 406 184 135 118 92 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4069 6628 7596 7842 669 Size: 988 281 2131 2559 968 246 3079 Fragments arranged by size: 3079 2559 2131 988 968 281 246 HindIII A'AGCT_T Cuts at: 748 3806 6448 748 Size: 3058 2642 4552 Fragments arranged by size: 4552 3058 2642 HpaI GTT'AAC Cuts at: 4069 7842 4069 Size: 3773 6479 KasI G'GCGC_C Cuts at: 5483 5483 Size: 10252 KpnI G_GTAC'C Cuts at: 7577 7577 Size: 10252 MfeI C'AATT_G Cuts at: 7851 7851 Size: 10252 MluI A'CGCG_T Cuts at: 10128 10128 Size: 10252 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2052 2079 2806 3231 3288 4842 4889 5079 Size: 747 27 727 425 57 1554 47 190 Cuts at: 5079 9366 9550 1305 Size: 4287 184 2007 Fragments arranged by size: 4287 2007 1554 747 727 425 190 184 57 47 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 2471 3116 4829 6785 10 Size: 54 1425 343 639 645 1713 1956 3477 Fragments arranged by size: 3477 1956 1713 1425 645 639 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 2546 3651 4370 4835 5108 6110 Size: 648 1008 372 1105 719 465 273 1002 Cuts at: 6110 8210 8569 8728 518 Size: 2100 359 159 2042 Fragments arranged by size: 2100 2042 1105 1008 1002 719 648 465 372 359 273 159 MspA1I CmG'CkG Cuts at: 1606 2260 2623 2806 3039 3349 3651 3711 4402 Size: 654 363 183 233 310 302 60 691 Cuts at: 4402 5136 5200 5425 5521 5583 5650 6092 7277 Size: 734 64 225 96 62 67 442 1185 Cuts at: 7277 7540 7668 8345 9493 9738 1606 Size: 263 128 677 1148 245 2120 Fragments arranged by size: 2120 1185 1148 734 691 677 654 442 363 310 302 263 245 233 225 183 128 96 67 64 62 60 NaeI GCC'GGC Cuts at: 2322 3215 4918 5593 5782 7082 2322 Size: 893 1703 675 189 1300 5492 Fragments arranged by size: 5492 1703 1300 893 675 189 NarI GG'CG_CC Cuts at: 5484 5484 Size: 10252 NcoI C'CATG_G Cuts at: 513 1065 6144 513 Size: 552 5079 4621 Fragments arranged by size: 5079 4621 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10252 NgoMIV G'CCGG_C Cuts at: 2320 3213 4916 5591 5780 7080 2320 Size: 893 1703 675 189 1300 5492 Fragments arranged by size: 5492 1703 1300 893 675 189 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10252 NotI GC'GGCC_GC Cuts at: 7699 7699 Size: 10252 NsiI A_TGCA'T Cuts at: 2219 2507 2219 Size: 288 9964 NspI r_CATG'y Cuts at: 1132 1229 3494 5899 7610 9155 1132 Size: 97 2265 2405 1711 1545 2229 Fragments arranged by size: 2405 2265 2229 1711 1545 97 PasI CC'CwG_GG Cuts at: 1348 3765 5454 5542 1348 Size: 2417 1689 88 6058 Fragments arranged by size: 6058 2417 1689 88 PciI A'CATG_T Cuts at: 9151 9151 Size: 10252 PflMI CCAn_nnn'nTGG Cuts at: 1108 2244 5476 6816 1108 Size: 1136 3232 1340 4544 Fragments arranged by size: 4544 3232 1340 1136 PfoI T'CCnGG_A Cuts at: 2349 2349 Size: 10252 PmeI GTTT'AAAC Cuts at: 7556 7556 Size: 10252 PmlI CAC'GTG Cuts at: 2935 2935 Size: 10252 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8321 8353 9834 9866 8321 Size: 32 1481 32 8707 Fragments arranged by size: 8707 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3250 5702 5816 6804 7104 7474 3250 Size: 2452 114 988 300 370 6028 Fragments arranged by size: 6028 2452 988 370 300 114 PshAI GACnn'nnGTC Cuts at: 1613 3884 1613 Size: 2271 7981 PsiI TTA'TAA Cuts at: 2781 7822 2781 Size: 5041 5211 PspOMI G'GGCC_C Cuts at: 3413 3554 5546 3413 Size: 141 1992 8119 Fragments arranged by size: 8119 1992 141 PspXI vC'TCGA_Gb Cuts at: 4524 7497 4524 Size: 2973 7279 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4433 4465 4433 Size: 32 10220 PstI C_TGCA'G Cuts at: 830 2205 2924 3357 3894 5991 6385 7077 7467 Size: 1375 719 433 537 2097 394 692 390 Cuts at: 7467 7604 830 Size: 137 3478 Fragments arranged by size: 3478 2097 1375 719 692 537 433 394 390 137 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10252 PvuII CAG'CTG Cuts at: 2623 3039 3349 5200 5425 7540 2623 Size: 416 310 1851 225 2115 5335 Fragments arranged by size: 5335 2115 1851 416 310 225 RsrII CG'GwC_CG Cuts at: 4297 4297 Size: 10252 SacI G_AGCT'C Cuts at: 2671 4281 2671 Size: 1610 8642 SacII CC_GC'GG Cuts at: 3652 3712 3652 Size: 60 10192 SalI G'TCGA_C Cuts at: 1936 7594 1936 Size: 5658 4594 SanDI GG'GwC_CC Cuts at: 5702 5816 5702 Size: 114 10138 SapI GCTCTTCn'nnn_ Cuts at: 3599 4584 3599 Size: 985 9267 SbfI CC_TGCA'GG Cuts at: 2924 5991 7604 2924 Size: 3067 1613 5572 Fragments arranged by size: 5572 3067 1613 ScaI AGT'ACT Cuts at: 8500 10212 8500 Size: 1712 8540 SexAI A'CCwGG_T Cuts at: 4137 4811 4137 Size: 674 9578 SfcI C'TryA_G Cuts at: 826 1045 2201 2920 3353 3571 3890 3989 4903 Size: 219 1156 719 433 218 319 99 914 Cuts at: 4903 5987 6381 6896 7073 7463 7600 8735 9416 Size: 1084 394 515 177 390 137 1135 681 Cuts at: 9416 9607 826 Size: 191 1471 Fragments arranged by size: 1471 1156 1135 1084 914 719 681 515 433 394 390 319 219 218 191 177 137 99 SfiI GGCCn_nnn'nGGCC Cuts at: 5723 5723 Size: 10252 SfoI GGC'GCC Cuts at: 5485 5485 Size: 10252 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10252 SgrAI Cr'CCGG_yG Cuts at: 1700 3213 1700 Size: 1513 8739 SmaI CCC'GGG Cuts at: 1872 3248 3412 3552 3586 5599 5813 7579 1872 Size: 1376 164 140 34 2013 214 1766 4545 Fragments arranged by size: 4545 2013 1766 1376 214 164 140 34 SmlI C'TyrA_G Cuts at: 820 1017 1943 4145 4524 6515 7497 8356 9257 Size: 197 926 2202 379 1991 982 859 901 Cuts at: 9257 9519 820 Size: 262 1553 Fragments arranged by size: 2202 1991 1553 982 926 901 859 379 262 197 SnaBI TAC'GTA Cuts at: 493 8043 493 Size: 7550 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10252 SphI G_CATG'C Cuts at: 1132 7610 1132 Size: 6478 3774 SrfI GCCC'GGGC Cuts at: 3412 3412 Size: 10252 SspI AAT'ATT Cuts at: 5 52 8176 5 Size: 47 8124 2081 Fragments arranged by size: 8124 2081 47 StuI AGG'CCT Cuts at: 1517 2562 2918 7421 1517 Size: 1045 356 4503 4348 Fragments arranged by size: 4503 4348 1045 356 StyI C'CwwG_G Cuts at: 513 1065 4459 4794 4884 5721 6144 6786 6800 Size: 552 3394 335 90 837 423 642 14 Cuts at: 6800 7691 513 Size: 891 3074 Fragments arranged by size: 3394 3074 891 837 642 552 423 335 90 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2381 2571 3964 4285 5468 5628 5691 Size: 1255 801 190 1393 321 1183 160 63 Cuts at: 5691 5870 7394 8279 8296 8449 8634 9079 325 Size: 179 1524 885 17 153 185 445 1498 Fragments arranged by size: 1524 1498 1393 1255 1183 885 801 445 321 190 185 179 160 153 63 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2270 4854 8498 Size: 274 80 33 51 1443 293 2584 3644 Cuts at: 8498 10210 96 Size: 1712 138 Fragments arranged by size: 3644 2584 1712 1443 293 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3740 3772 3963 3995 4455 4487 6567 6599 7183 Size: 32 191 32 460 32 2080 32 584 Cuts at: 7183 7215 10212 10244 3740 Size: 32 2997 32 3748 Fragments arranged by size: 3748 2997 2080 584 460 191 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3659 6458 1316 Size: 2343 2799 5110 Fragments arranged by size: 5110 2799 2343 XbaI T'CTAG_A Cuts at: 7588 7588 Size: 10252 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5477 5647 6133 7247 5477 Size: 170 486 1114 8482 Fragments arranged by size: 8482 1114 486 170 XhoI C'TCGA_G Cuts at: 1943 4524 6515 7497 1943 Size: 2581 1991 982 4698 Fragments arranged by size: 4698 2581 1991 982 XmaI C'CCGG_G Cuts at: 1870 3246 3410 3550 3584 5597 5811 7577 1870 Size: 1376 164 140 34 2013 214 1766 4545 Fragments arranged by size: 4545 2013 1766 1376 214 164 140 34 XmnI GAAnn'nnTTC Cuts at: 3809 8381 3809 Size: 4572 5680 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6826 8060 276 Size: 53 83 186 936 5292 1234 2468 Fragments arranged by size: 5292 2468 1234 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BplI BsiWI BstZ17I FalI FseI FspAI NruI PacI SwaI