(Circular) (Six-base) MAPSORT of: pFN21AE2797.seq Check: 5286 from: 1 to: 7078 pFN21AE2797 7078 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2727 4888 278 Size: 53 83 186 936 1191 2161 2468 Fragments arranged by size: 2468 2161 1191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3228 4421 1937 Size: 1291 1193 4594 Fragments arranged by size: 4594 1291 1193 Acc65I G'GTAC_C Cuts at: 4399 4399 Size: 7078 AclI AA'CG_TT Cuts at: 1563 5205 5578 1563 Size: 3642 373 3063 Fragments arranged by size: 3642 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2381 2607 3095 3788 3862 5141 6525 6793 Size: 749 226 488 693 74 1279 1384 268 Cuts at: 6793 1632 Size: 1917 Fragments arranged by size: 1917 1384 1279 749 693 488 268 226 74 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6881 AflIII A'CryG_T Cuts at: 1203 3321 5977 6954 1203 Size: 2118 2656 977 1327 Fragments arranged by size: 2656 2118 1327 977 AgeI A'CCGG_T Cuts at: 5909 5909 Size: 7078 AhdI GACnn_n'nnGTC Cuts at: 5807 5807 Size: 7078 AleI CACnn'nnGTG Cuts at: 1166 3185 1166 Size: 2019 5059 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7078 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3551 3583 3551 Size: 32 7046 AlwNI CAG_nnn'CTG Cuts at: 2845 4821 6393 2845 Size: 1976 1572 3530 Fragments arranged by size: 3530 1976 1572 ApaLI G'TGCA_C Cuts at: 2124 2316 5135 6291 2124 Size: 192 2819 1156 2911 Fragments arranged by size: 2911 2819 1156 192 ApoI r'AATT_y Cuts at: 1493 4358 4387 4613 4765 6874 1493 Size: 2865 29 226 152 2109 1697 Fragments arranged by size: 2865 2109 1697 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 5632 160 Size: 872 4600 1606 Fragments arranged by size: 4600 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7078 AvaI C'yCGr_G Cuts at: 1870 1943 2828 3393 4261 4403 1870 Size: 73 885 565 868 142 4545 Fragments arranged by size: 4545 885 868 565 142 73 AvrII C'CTAG_G Cuts at: 3457 3457 Size: 7078 BamHI G'GATC_C Cuts at: 2568 4408 4775 2568 Size: 1840 367 4871 Fragments arranged by size: 4871 1840 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2448 2717 4080 4399 Size: 325 217 627 242 419 269 1363 319 Cuts at: 4399 5854 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1363 627 419 325 319 269 242 217 BanII G_rGCy'C Cuts at: 1391 4397 1391 Size: 3006 4072 BbeI G_GCGC'C Cuts at: 2452 2721 4084 2452 Size: 269 1363 5446 Fragments arranged by size: 5446 1363 269 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2185 2516 3607 4238 4263 928 Size: 971 286 331 1091 631 25 3743 Fragments arranged by size: 3743 1091 971 631 331 286 25 BbvCI CC'TCA_GC Cuts at: 4136 4136 Size: 7078 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5269 5303 1903 Size: 34 3332 34 3678 Fragments arranged by size: 3678 3332 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3963 4970 6180 3963 Size: 1007 1210 4861 Fragments arranged by size: 4861 1210 1007 BclI T'GATC_A Cuts at: 1548 1548 Size: 7078 BfrBI ATG'CAT Cuts at: 4340 4340 Size: 7078 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2744 5689 136 Size: 107 122 71 631 605 1072 2945 1525 Fragments arranged by size: 2945 1525 1072 631 605 122 107 71 BglII A'GATC_T Cuts at: 7073 7073 Size: 7078 BlpI GC'TnA_GC Cuts at: 4495 4495 Size: 7078 Bme1580I G_kGCm'C Cuts at: 1790 2128 2320 5139 6295 1790 Size: 338 192 2819 1156 2573 Fragments arranged by size: 2819 2573 1156 338 192 BmgBI CAC'GTC Cuts at: 1318 4240 1318 Size: 2922 4156 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2055 2257 2307 Size: 416 53 83 399 24 638 202 50 Cuts at: 2307 3004 3151 4809 5767 442 Size: 697 147 1658 958 1753 Fragments arranged by size: 1753 1658 958 697 638 416 399 202 147 83 53 50 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7078 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4072 4072 Size: 7078 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 3023 3290 5738 1239 Size: 920 864 267 2448 2579 Fragments arranged by size: 2579 2448 920 864 267 Bpu10I CC'TnA_GC Cuts at: 4136 4136 Size: 7078 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2256 5203 6068 6366 2256 Size: 2947 865 298 2968 Fragments arranged by size: 2968 2947 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2282 2999 3523 5741 882 Size: 617 783 717 524 2218 2219 Fragments arranged by size: 2219 2218 783 717 617 524 BsaAI yAC'GTr Cuts at: 493 1204 4869 493 Size: 711 3665 2702 Fragments arranged by size: 3665 2702 711 BsaBI GATnn'nnATC Cuts at: 7072 7072 Size: 7078 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2449 2718 2724 2795 Size: 53 83 186 936 916 269 6 71 Cuts at: 2795 4081 4885 5267 5902 275 Size: 1286 804 382 635 1451 Fragments arranged by size: 1451 1286 936 916 804 635 382 269 186 83 71 53 6 BsaWI w'CCGG_w Cuts at: 3114 4149 5511 5909 6183 6330 6741 3114 Size: 1035 1362 398 274 147 411 3451 Fragments arranged by size: 3451 1362 1035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2170 2200 2240 2270 3848 Size: 30 165 30 767 30 40 30 1578 Cuts at: 3848 3878 1178 Size: 30 4378 Fragments arranged by size: 4378 1578 767 165 40 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2175 2259 2356 2417 2648 3413 3416 3998 Size: 989 84 97 61 231 765 3 582 Cuts at: 3998 4001 4153 1186 Size: 3 152 4111 Fragments arranged by size: 4111 989 765 582 231 152 97 84 61 3 3 BseYI C'CCAG_C Cuts at: 3678 6281 6777 3678 Size: 2603 496 3979 Fragments arranged by size: 3979 2603 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2858 3263 1163 Size: 573 1122 405 4978 Fragments arranged by size: 4978 1122 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4528 5289 6317 1540 Size: 275 186 2527 761 1028 2301 Fragments arranged by size: 2527 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2128 2320 2856 3477 5139 5224 6295 2128 Size: 192 536 621 1662 85 1071 2911 Fragments arranged by size: 2911 1662 1071 621 536 192 85 BsiWI C'GTAC_G Cuts at: 3147 3147 Size: 7078 BsmI GAATG_Cn' Cuts at: 4589 4682 4589 Size: 93 6985 BsmBI CGTCTCn'nnnn_ Cuts at: 2786 3382 4060 4246 2786 Size: 596 678 186 5618 Fragments arranged by size: 5618 678 596 186 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 2320 2856 3477 4397 5139 5224 Size: 399 338 192 536 621 920 742 85 Cuts at: 5224 6295 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 920 742 621 536 399 338 192 85 BspEI T'CCGG_A Cuts at: 3114 4149 6741 3114 Size: 1035 2592 3451 Fragments arranged by size: 3451 2592 1035 BspHI T'CATG_A Cuts at: 3735 4967 6697 3735 Size: 1232 1730 4116 Fragments arranged by size: 4116 1730 1232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2522 2598 4433 844 Size: 1678 76 1835 3489 Fragments arranged by size: 3489 1835 1678 76 BsrBI CCG'CTC Cuts at: 1394 1823 2677 4965 1394 Size: 429 854 2288 3507 Fragments arranged by size: 3507 2288 854 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3510 4023 5573 5747 65 Size: 1183 2262 513 1550 174 1396 Fragments arranged by size: 2262 1550 1396 1183 513 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2095 2714 3249 5722 5909 1700 Size: 110 285 619 535 2473 187 2869 Fragments arranged by size: 2869 2473 619 535 285 187 110 BsrGI T'GTAC_A Cuts at: 96 3726 96 Size: 3630 3448 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7078 BssSI C'ACGA_G Cuts at: 2855 2886 3656 5138 6150 2855 Size: 31 770 1482 1012 3783 Fragments arranged by size: 3783 1482 1012 770 31 BstAPI GCAn_nnn'nTGC Cuts at: 794 3259 3286 794 Size: 2465 27 4586 Fragments arranged by size: 4586 2465 27 BstBI TT'CG_AA Cuts at: 5882 5882 Size: 7078 BstEII G'GTnAC_C Cuts at: 1184 2914 1184 Size: 1730 5348 BstXI CCAn_nnnn'nTGG Cuts at: 2026 2206 3673 3919 2026 Size: 180 1467 246 5185 Fragments arranged by size: 5185 1467 246 180 BstYI r'GATC_y Cuts at: 1972 2568 3069 3538 4408 4775 5160 5177 6629 Size: 596 501 469 870 367 385 17 1452 Cuts at: 6629 6715 6727 7073 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 870 596 501 469 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2205 2919 3198 3666 3717 513 Size: 552 1140 714 279 468 51 3874 Fragments arranged by size: 3874 1140 714 552 468 279 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2650 2677 2692 487 Size: 683 56 1424 27 15 4873 Fragments arranged by size: 4873 1424 683 56 27 15 BtsI GCAGTG_nn' Cuts at: 2231 3894 4594 5388 5408 2231 Size: 1663 700 794 20 3901 Fragments arranged by size: 3901 1663 794 700 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7078 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7043 DraI TTT'AAA Cuts at: 4382 4382 Size: 7078 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6085 809 Size: 507 4769 1802 Fragments arranged by size: 4769 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2325 2922 3045 Size: 54 1425 50 275 18 495 597 123 Cuts at: 3045 3663 4173 4525 5414 7043 8 Size: 618 510 352 889 1629 43 Fragments arranged by size: 1629 1425 889 618 597 510 495 352 275 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4525 1537 Size: 275 2713 4090 Fragments arranged by size: 4090 2713 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2114 2328 2583 3905 5008 1353 Size: 731 30 214 255 1322 1103 3423 Fragments arranged by size: 3423 1322 1103 731 255 214 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2114 3080 5665 6039 6185 387 Size: 1064 138 525 966 2585 374 146 1280 Fragments arranged by size: 2585 1280 1064 966 525 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2381 2607 3023 3095 3290 3788 Size: 393 527 222 226 416 72 195 498 Cuts at: 3788 3862 5141 5738 6525 6793 1239 Size: 74 1279 597 787 268 1524 Fragments arranged by size: 1524 1279 787 597 527 498 416 393 268 226 222 195 74 72 EcoNI CCTnn'n_nnAGG Cuts at: 2522 2522 Size: 7078 EcoO109I rG'GnC_Cy Cuts at: 2745 4265 2745 Size: 1520 5558 EcoRI G'AATT_C Cuts at: 4358 4387 4765 4358 Size: 29 378 6671 Fragments arranged by size: 6671 378 29 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2389 2764 3614 2389 Size: 375 850 5853 Fragments arranged by size: 5853 850 375 FspI TGC'GCA Cuts at: 5584 5584 Size: 7078 HaeII r_GCGC'y Cuts at: 1678 2452 2660 2721 4084 6225 1678 Size: 774 208 61 1363 2141 2531 Fragments arranged by size: 2531 2141 1363 774 208 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2017 2049 2170 2202 2240 Size: 32 135 32 445 32 121 32 38 Cuts at: 2240 2272 2279 2311 2858 2890 3530 3562 3848 Size: 32 7 32 547 32 640 32 286 Cuts at: 3848 3880 5719 5751 5793 5825 1373 Size: 32 1839 32 42 32 2626 Fragments arranged by size: 2626 1839 640 547 445 286 135 121 42 38 32 32 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2728 4422 4668 669 Size: 988 281 790 1694 246 3079 Fragments arranged by size: 3079 1694 988 790 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7078 HpaI GTT'AAC Cuts at: 4668 4668 Size: 7078 KasI G'GCGC_C Cuts at: 2448 2717 4080 2448 Size: 269 1363 5446 Fragments arranged by size: 5446 1363 269 KpnI G_GTAC'C Cuts at: 4403 4403 Size: 7078 MfeI C'AATT_G Cuts at: 4677 4677 Size: 7078 MluI A'CGCG_T Cuts at: 6954 6954 Size: 7078 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2238 2523 4213 6192 6376 1305 Size: 933 285 1690 1979 184 2007 Fragments arranged by size: 2007 1979 1690 933 285 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 3665 10 Size: 54 1425 343 495 1338 3423 Fragments arranged by size: 3423 1425 1338 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3185 3497 3722 5036 5395 5554 518 Size: 648 2019 312 225 1314 359 159 2042 Fragments arranged by size: 2042 2019 1314 648 359 312 225 159 MspA1I CmG'CkG Cuts at: 1606 2323 2842 3037 3043 3277 4494 5171 6319 Size: 717 519 195 6 234 1217 677 1148 Cuts at: 6319 6564 1606 Size: 245 2120 Fragments arranged by size: 2120 1217 1148 717 677 519 245 234 195 6 NaeI GCC'GGC Cuts at: 2097 2716 2097 Size: 619 6459 NarI GG'CG_CC Cuts at: 2449 2718 4081 2449 Size: 269 1363 5446 Fragments arranged by size: 5446 1363 269 NcoI C'CATG_G Cuts at: 513 1065 2205 3666 3717 513 Size: 552 1140 1461 51 3874 Fragments arranged by size: 3874 1461 1140 552 51 NdeI CA'TA_TG Cuts at: 387 3294 387 Size: 2907 4171 NgoMIV G'CCGG_C Cuts at: 2095 2714 2095 Size: 619 6459 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7078 NotI GC'GGCC_GC Cuts at: 4525 4525 Size: 7078 NsiI A_TGCA'T Cuts at: 4342 4342 Size: 7078 NspI r_CATG'y Cuts at: 1132 1229 2817 2965 3325 3691 3727 4021 4436 Size: 97 1588 148 360 366 36 294 415 Cuts at: 4436 5981 1132 Size: 1545 2229 Fragments arranged by size: 2229 1588 1545 415 366 360 294 148 97 36 PasI CC'CwG_GG Cuts at: 1348 3673 1348 Size: 2325 4753 PciI A'CATG_T Cuts at: 3321 5977 3321 Size: 2656 4422 PflMI CCAn_nnn'nTGG Cuts at: 1108 3718 1108 Size: 2610 4468 PfoI T'CCnGG_A Cuts at: 2136 3125 4310 4362 2136 Size: 989 1185 52 4852 Fragments arranged by size: 4852 1185 989 52 PmeI GTTT'AAAC Cuts at: 4382 4382 Size: 7078 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5147 5179 6660 6692 5147 Size: 32 1481 32 5533 Fragments arranged by size: 5533 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3157 3374 4238 1613 Size: 1544 217 864 4453 Fragments arranged by size: 4453 1544 864 217 PsiI TTA'TAA Cuts at: 4648 4648 Size: 7078 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3677 3709 3677 Size: 32 7046 PstI C_TGCA'G Cuts at: 830 2433 3042 3453 3546 3863 4089 4430 830 Size: 1603 609 411 93 317 226 341 3478 Fragments arranged by size: 3478 1603 609 411 341 317 226 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7078 PvuII CAG'CTG Cuts at: 2323 2842 3043 2323 Size: 519 201 6358 Fragments arranged by size: 6358 519 201 SalI G'TCGA_C Cuts at: 1936 4420 1936 Size: 2484 4594 SapI GCTCTTCn'nnn_ Cuts at: 2328 2328 Size: 7078 SbfI CC_TGCA'GG Cuts at: 4430 4430 Size: 7078 ScaI AGT'ACT Cuts at: 5326 7038 5326 Size: 1712 5366 SfcI C'TryA_G Cuts at: 826 1045 2429 3038 3449 3542 3859 4085 4426 Size: 219 1384 609 411 93 317 226 341 Cuts at: 4426 5561 6242 6433 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1384 1135 681 609 411 341 317 226 219 191 93 SfoI GGC'GCC Cuts at: 2450 2719 4082 2450 Size: 269 1363 5446 Fragments arranged by size: 5446 1363 269 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7078 SgrAI Cr'CCGG_yG Cuts at: 1700 2714 1700 Size: 1014 6064 SmaI CCC'GGG Cuts at: 1872 3395 4263 4405 1872 Size: 1523 868 142 4545 Fragments arranged by size: 4545 1523 868 142 SmlI C'TyrA_G Cuts at: 820 1017 1943 2271 5182 6083 6345 820 Size: 197 926 328 2911 901 262 1553 Fragments arranged by size: 2911 1553 926 901 328 262 197 SnaBI TAC'GTA Cuts at: 493 4869 493 Size: 4376 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7078 SphI G_CATG'C Cuts at: 1132 2817 4436 1132 Size: 1685 1619 3774 Fragments arranged by size: 3774 1685 1619 SspI AAT'ATT Cuts at: 5 52 5002 5 Size: 47 4950 2081 Fragments arranged by size: 4950 2081 47 StuI AGG'CCT Cuts at: 1517 2528 2639 3918 1517 Size: 1011 111 1279 4677 Fragments arranged by size: 4677 1279 1011 111 StyI C'CwwG_G Cuts at: 513 1065 2019 2205 2328 2553 2739 3420 3457 Size: 552 954 186 123 225 186 681 37 Cuts at: 3457 3666 3717 3816 4059 4517 513 Size: 209 51 99 243 458 3074 Fragments arranged by size: 3074 954 681 552 458 243 225 209 186 186 123 99 51 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3911 5105 5122 5275 5460 5905 325 Size: 1255 2331 1194 17 153 185 445 1498 Fragments arranged by size: 2331 1498 1255 1194 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2625 3078 3621 Size: 274 80 33 51 1443 648 453 543 Cuts at: 3621 3726 5324 7036 96 Size: 105 1598 1712 138 Fragments arranged by size: 1712 1598 1443 648 543 453 274 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2118 2150 7038 7070 2118 Size: 32 4888 32 2126 Fragments arranged by size: 4888 2126 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7078 XbaI T'CTAG_A Cuts at: 4414 4414 Size: 7078 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2320 4102 2320 Size: 1782 5296 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7078 XmaI C'CCGG_G Cuts at: 1870 3393 4261 4403 1870 Size: 1523 868 142 4545 Fragments arranged by size: 4545 1523 868 142 XmnI GAAnn'nnTTC Cuts at: 5207 5207 Size: 7078 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2725 4886 276 Size: 53 83 186 936 1191 2161 2468 Fragments arranged by size: 2468 2161 1191 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI