(Linear) (Six-base) MAPSORT of: KIEE2797.seq Check: 3481 from: 1 to: 2373 KIEE2797.orf With 182 enzymes: SgfI * August 16, 2021 14:16 .. AatII G_ACGT'C Cuts at: 0 722 2373 Size: 722 1651 AccI GT'mk_AC Cuts at: 0 1223 2373 Size: 1223 1150 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 602 1090 1783 1857 2373 Size: 376 226 488 693 74 516 Fragments arranged by size: 693 516 488 376 226 74 AflIII A'CryG_T Cuts at: 0 1316 2373 Size: 1316 1057 AleI CACnn'nnGTG Cuts at: 0 1180 2373 Size: 1180 1193 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1546 1578 2373 Size: 1546 32 795 Fragments arranged by size: 1546 795 32 AlwNI CAG_nnn'CTG Cuts at: 0 840 2373 Size: 840 1533 ApaLI G'TGCA_C Cuts at: 0 119 311 2373 Size: 119 192 2062 Fragments arranged by size: 2062 192 119 ApoI r'AATT_y Cuts at: 0 2353 2373 Size: 2353 20 AvaI C'yCGr_G Cuts at: 0 823 1388 2256 2373 Size: 823 565 868 117 Fragments arranged by size: 868 823 565 117 AvrII C'CTAG_G Cuts at: 0 1452 2373 Size: 1452 921 BamHI G'GATC_C Cuts at: 0 563 2373 Size: 563 1810 BanI G'GyrC_C Cuts at: 0 24 443 712 2075 2373 Size: 24 419 269 1363 298 Fragments arranged by size: 1363 419 298 269 24 BbeI G_GCGC'C Cuts at: 0 447 716 2079 2373 Size: 447 269 1363 294 Fragments arranged by size: 1363 447 294 269 BbsI GAAGACnn'nnnn_ Cuts at: 0 180 511 1602 2233 2258 2373 Size: 180 331 1091 631 25 115 Fragments arranged by size: 1091 631 331 180 115 25 BbvCI CC'TCA_GC Cuts at: 0 2131 2373 Size: 2131 242 BciVI GTATCCnnnnn_n' Cuts at: 0 1958 2373 Size: 1958 415 BfrBI ATG'CAT Cuts at: 0 2335 2373 Size: 2335 38 BglI GCCn_nnn'nGGC Cuts at: 0 739 2373 Size: 739 1634 Bme1580I G_kGCm'C Cuts at: 0 123 315 2373 Size: 123 192 2058 Fragments arranged by size: 2058 192 123 BmgBI CAC'GTC Cuts at: 0 2235 2373 Size: 2235 138 BmrI ACTGGGnnnn_n' Cuts at: 0 50 252 302 999 1146 2373 Size: 50 202 50 697 147 1227 Fragments arranged by size: 1227 697 202 147 50 50 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2067 2373 Size: 2067 306 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 1018 1285 2373 Size: 154 864 267 1088 Fragments arranged by size: 1088 864 267 154 Bpu10I CC'TnA_GC Cuts at: 0 2131 2373 Size: 2131 242 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 2373 Size: 251 2122 BsaI GGTCTCn'nnnn_ Cuts at: 0 277 994 1518 2373 Size: 277 717 524 855 Fragments arranged by size: 855 717 524 277 BsaHI Gr'CG_yC Cuts at: 0 444 713 719 790 2076 2373 Size: 444 269 6 71 1286 297 Fragments arranged by size: 1286 444 297 269 71 6 BsaWI w'CCGG_w Cuts at: 0 1109 2144 2373 Size: 1109 1035 229 Fragments arranged by size: 1109 1035 229 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 165 195 235 265 1843 1873 2373 Size: 165 30 40 30 1578 30 500 Fragments arranged by size: 1578 500 165 40 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 170 254 351 412 643 1408 1411 1993 Size: 170 84 97 61 231 765 3 582 Cuts at: 1993 1996 2148 2373 Size: 3 152 225 Fragments arranged by size: 765 582 231 225 170 152 97 84 61 3 3 BseYI C'CCAG_C Cuts at: 0 1673 2373 Size: 1673 700 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 853 1258 2373 Size: 853 405 1115 Fragments arranged by size: 1115 853 405 BsiHKAI G_wGCw'C Cuts at: 0 123 315 851 1472 2373 Size: 123 192 536 621 901 Fragments arranged by size: 901 621 536 192 123 BsiWI C'GTAC_G Cuts at: 0 1142 2373 Size: 1142 1231 BsmBI CGTCTCn'nnnn_ Cuts at: 0 781 1377 2055 2241 2373 Size: 781 596 678 186 132 Fragments arranged by size: 781 678 596 186 132 Bsp1286I G_dGCh'C Cuts at: 0 123 315 851 1472 2373 Size: 123 192 536 621 901 Fragments arranged by size: 901 621 536 192 123 BspEI T'CCGG_A Cuts at: 0 1109 2144 2373 Size: 1109 1035 229 Fragments arranged by size: 1109 1035 229 BspHI T'CATG_A Cuts at: 0 1730 2373 Size: 1730 643 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 517 593 2373 Size: 517 76 1780 Fragments arranged by size: 1780 517 76 BsrBI CCG'CTC Cuts at: 0 672 2373 Size: 672 1701 BsrDI GCAATG_nn' Cuts at: 0 1505 2018 2373 Size: 1505 513 355 Fragments arranged by size: 1505 513 355 BsrFI r'CCGG_y Cuts at: 0 90 709 1244 2373 Size: 90 619 535 1129 Fragments arranged by size: 1129 619 535 90 BsrGI T'GTAC_A Cuts at: 0 1721 2373 Size: 1721 652 BssSI C'ACGA_G Cuts at: 0 850 881 1651 2373 Size: 850 31 770 722 Fragments arranged by size: 850 770 722 31 BstAPI GCAn_nnn'nTGC Cuts at: 0 1254 1281 2373 Size: 1254 27 1092 Fragments arranged by size: 1254 1092 27 BstEII G'GTnAC_C Cuts at: 0 909 2373 Size: 909 1464 BstXI CCAn_nnnn'nTGG Cuts at: 0 21 201 1668 1914 2373 Size: 21 180 1467 246 459 Fragments arranged by size: 1467 459 246 180 21 BstYI r'GATC_y Cuts at: 0 563 1064 1533 2373 Size: 563 501 469 840 Fragments arranged by size: 840 563 501 469 BtgI C'CryG_G Cuts at: 0 200 914 1193 1661 1712 2373 Size: 200 714 279 468 51 661 Fragments arranged by size: 714 661 468 279 200 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 645 672 687 2373 Size: 645 27 15 1686 Fragments arranged by size: 1686 645 27 15 BtsI GCAGTG_nn' Cuts at: 0 226 1889 2373 Size: 226 1663 484 Fragments arranged by size: 1663 484 226 EaeI y'GGCC_r Cuts at: 0 320 917 1040 1658 2168 2373 Size: 320 597 123 618 510 205 Fragments arranged by size: 618 597 510 320 205 123 EarI CTCTTCn'nnn_ Cuts at: 0 79 109 323 578 1900 2373 Size: 79 30 214 255 1322 473 Fragments arranged by size: 1322 473 255 214 79 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 109 1075 2373 Size: 109 966 1298 Fragments arranged by size: 1298 966 109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 376 602 1018 1090 1285 1783 1857 Size: 154 222 226 416 72 195 498 74 Cuts at: 1857 2373 Size: 516 Fragments arranged by size: 516 498 416 226 222 195 154 74 72 EcoNI CCTnn'n_nnAGG Cuts at: 0 517 2373 Size: 517 1856 EcoO109I rG'GnC_Cy Cuts at: 0 740 2260 2373 Size: 740 1520 113 Fragments arranged by size: 1520 740 113 EcoRI G'AATT_C Cuts at: 0 2353 2373 Size: 2353 20 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 384 759 1609 2373 Size: 384 375 850 764 Fragments arranged by size: 850 764 384 375 HaeII r_GCGC'y Cuts at: 0 447 655 716 2079 2373 Size: 447 208 61 1363 294 Fragments arranged by size: 1363 447 294 208 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 12 44 165 197 235 267 274 306 Size: 12 32 121 32 38 32 7 32 Cuts at: 306 853 885 1525 1557 1843 1875 2373 Size: 547 32 640 32 286 32 498 Fragments arranged by size: 640 547 498 286 121 38 32 32 32 32 32 32 32 12 7 HincII GTy'rAC Cuts at: 0 723 2373 Size: 723 1650 KasI G'GCGC_C Cuts at: 0 443 712 2075 2373 Size: 443 269 1363 298 Fragments arranged by size: 1363 443 298 269 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 233 518 2208 2373 Size: 233 285 1690 165 Fragments arranged by size: 1690 285 233 165 MscI TGG'CCA Cuts at: 0 322 1660 2373 Size: 322 1338 713 Fragments arranged by size: 1338 713 322 MslI CAynn'nnrTG Cuts at: 0 1180 1492 1717 2373 Size: 1180 312 225 656 Fragments arranged by size: 1180 656 312 225 MspA1I CmG'CkG Cuts at: 0 318 837 1032 1038 1272 2373 Size: 318 519 195 6 234 1101 Fragments arranged by size: 1101 519 318 234 195 6 NaeI GCC'GGC Cuts at: 0 92 711 2373 Size: 92 619 1662 Fragments arranged by size: 1662 619 92 NarI GG'CG_CC Cuts at: 0 444 713 2076 2373 Size: 444 269 1363 297 Fragments arranged by size: 1363 444 297 269 NcoI C'CATG_G Cuts at: 0 200 1661 1712 2373 Size: 200 1461 51 661 Fragments arranged by size: 1461 661 200 51 NdeI CA'TA_TG Cuts at: 0 1289 2373 Size: 1289 1084 NgoMIV G'CCGG_C Cuts at: 0 90 709 2373 Size: 90 619 1664 Fragments arranged by size: 1664 619 90 NsiI A_TGCA'T Cuts at: 0 2337 2373 Size: 2337 36 NspI r_CATG'y Cuts at: 0 812 960 1320 1686 1722 2016 2373 Size: 812 148 360 366 36 294 357 Fragments arranged by size: 812 366 360 357 294 148 36 PasI CC'CwG_GG Cuts at: 0 1668 2373 Size: 1668 705 PciI A'CATG_T Cuts at: 0 1316 2373 Size: 1316 1057 PflMI CCAn_nnn'nTGG Cuts at: 0 1713 2373 Size: 1713 660 PfoI T'CCnGG_A Cuts at: 0 131 1120 2305 2357 2373 Size: 131 989 1185 52 16 Fragments arranged by size: 1185 989 131 52 16 PshAI GACnn'nnGTC Cuts at: 0 1152 1369 2233 2373 Size: 1152 217 864 140 Fragments arranged by size: 1152 864 217 140 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1672 1704 2373 Size: 1672 32 669 Fragments arranged by size: 1672 669 32 PstI C_TGCA'G Cuts at: 0 428 1037 1448 1541 1858 2084 2373 Size: 428 609 411 93 317 226 289 Fragments arranged by size: 609 428 411 317 289 226 93 PvuII CAG'CTG Cuts at: 0 318 837 1038 2373 Size: 318 519 201 1335 Fragments arranged by size: 1335 519 318 201 SapI GCTCTTCn'nnn_ Cuts at: 0 323 2373 Size: 323 2050 SfcI C'TryA_G Cuts at: 0 424 1033 1444 1537 1854 2080 2373 Size: 424 609 411 93 317 226 293 Fragments arranged by size: 609 424 411 317 293 226 93 SfoI GGC'GCC Cuts at: 0 445 714 2077 2373 Size: 445 269 1363 296 Fragments arranged by size: 1363 445 296 269 SgrAI Cr'CCGG_yG Cuts at: 0 709 2373 Size: 709 1664 SmaI CCC'GGG Cuts at: 0 1390 2258 2373 Size: 1390 868 115 Fragments arranged by size: 1390 868 115 SmlI C'TyrA_G Cuts at: 0 266 2373 Size: 266 2107 SphI G_CATG'C Cuts at: 0 812 2373 Size: 812 1561 StuI AGG'CCT Cuts at: 0 523 634 1913 2373 Size: 523 111 1279 460 Fragments arranged by size: 1279 523 460 111 StyI C'CwwG_G Cuts at: 0 14 200 323 548 734 1415 1452 1661 Size: 14 186 123 225 186 681 37 209 Cuts at: 1661 1712 1811 2054 2373 Size: 51 99 243 319 Fragments arranged by size: 681 319 243 225 209 186 186 123 99 51 37 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1906 2373 Size: 1906 467 TatI w'GTAC_w Cuts at: 0 620 1073 1616 1721 2373 Size: 620 453 543 105 652 Fragments arranged by size: 652 620 543 453 105 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 113 145 2373 Size: 113 32 2228 Fragments arranged by size: 2228 113 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 315 2097 2373 Size: 315 1782 276 Fragments arranged by size: 1782 315 276 XmaI C'CCGG_G Cuts at: 0 1388 2256 2373 Size: 1388 868 117 Fragments arranged by size: 1388 868 117 ZraI GAC'GTC Cuts at: 0 720 2373 Size: 720 1653 Enzymes that do cut: AatII AccI AcuI AflIII AleI AloI AlwNI ApaLI ApoI AvaI AvrII BamHI BanI BbeI BbsI BbvCI BciVI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI PshAI PsrI PstI PvuII SapI SfcI SfoI SgrAI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaI AscI AseI AsiSI BaeI BanII BcgI BclI BglII BlpI BmtI BsaAI BsaBI BsiEI BsmI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NheI NotI NruI PacI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI