(Circular) (Six-base) MAPSORT of: pFN21AE3874.seq Check: 799 from: 1 to: 6802 pFN21AE3874 6802 bp With 182 enzymes: SgfI * August 4, 2021 21:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2119 2119 Size: 6802 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4612 278 Size: 53 83 186 936 3076 2468 Fragments arranged by size: 3076 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3903 4145 1937 Size: 1966 242 4594 Fragments arranged by size: 4594 1966 242 Acc65I G'GTAC_C Cuts at: 4123 4123 Size: 6802 AclI AA'CG_TT Cuts at: 1563 4929 5302 1563 Size: 3366 373 3063 Fragments arranged by size: 3366 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2781 3207 4865 6249 6517 1632 Size: 1149 426 1658 1384 268 1917 Fragments arranged by size: 1917 1658 1384 1149 426 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6605 AflIII A'CryG_T Cuts at: 1203 5701 6678 1203 Size: 4498 977 1327 Fragments arranged by size: 4498 1327 977 AgeI A'CCGG_T Cuts at: 3612 5633 3612 Size: 2021 4781 AhdI GACnn_n'nnGTC Cuts at: 5531 5531 Size: 6802 AleI CACnn'nnGTG Cuts at: 1166 2523 1166 Size: 1357 5445 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2690 3233 1184 Size: 1506 543 4753 Fragments arranged by size: 4753 1506 543 AlwNI CAG_nnn'CTG Cuts at: 2804 4545 6117 2804 Size: 1741 1572 3489 Fragments arranged by size: 3489 1741 1572 ApaI G_GGCC'C Cuts at: 2142 2697 2142 Size: 555 6247 ApaLI G'TGCA_C Cuts at: 4859 6015 4859 Size: 1156 5646 ApoI r'AATT_y Cuts at: 1493 2314 2780 3537 3892 4111 4337 4489 6598 Size: 821 466 757 355 219 226 152 2109 Cuts at: 6598 1493 Size: 1697 Fragments arranged by size: 2109 1697 821 757 466 355 226 219 152 AseI AT'TA_AT Cuts at: 160 1032 5356 160 Size: 872 4324 1606 Fragments arranged by size: 4324 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6802 AvaI C'yCGr_G Cuts at: 1870 1943 2208 2214 2444 2538 3411 4127 1870 Size: 73 265 6 230 94 873 716 4545 Fragments arranged by size: 4545 873 716 265 230 94 73 6 AvrII C'CTAG_G Cuts at: 3383 3383 Size: 6802 BamHI G'GATC_C Cuts at: 4132 4499 4132 Size: 367 6435 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3113 4123 5578 618 Size: 325 217 627 1326 1010 1455 1842 Fragments arranged by size: 1842 1455 1326 1010 627 325 217 BanII G_rGCy'C Cuts at: 1391 2142 2215 2697 2704 3971 4121 1391 Size: 751 73 482 7 1267 150 4072 Fragments arranged by size: 4072 1267 751 482 150 73 7 BbeI G_GCGC'C Cuts at: 3117 3117 Size: 6802 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3802 4054 928 Size: 971 1903 252 3676 Fragments arranged by size: 3676 1903 971 252 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4993 5027 1903 Size: 34 3056 34 3678 Fragments arranged by size: 3678 3056 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4694 5904 4694 Size: 1210 5592 BclI T'GATC_A Cuts at: 1548 3520 1548 Size: 1972 4830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5413 136 Size: 107 122 71 631 605 3741 1525 Fragments arranged by size: 3741 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6797 6797 Size: 6802 BlpI GC'TnA_GC Cuts at: 4219 4219 Size: 6802 Bme1580I G_kGCm'C Cuts at: 1790 2142 2697 3212 4863 6019 1790 Size: 352 555 515 1651 1156 2573 Fragments arranged by size: 2573 1651 1156 555 515 352 BmgBI CAC'GTC Cuts at: 1318 2635 1318 Size: 1317 5485 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4533 5491 442 Size: 416 53 83 399 24 3116 958 1753 Fragments arranged by size: 3116 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6802 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2359 2359 Size: 6802 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2879 3938 5462 1239 Size: 830 810 1059 1524 2579 Fragments arranged by size: 2579 1524 1059 830 810 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4927 5792 6090 4927 Size: 865 298 5639 Fragments arranged by size: 5639 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2156 2236 5465 882 Size: 617 657 80 3229 2219 Fragments arranged by size: 3229 2219 657 617 80 BsaAI yAC'GTr Cuts at: 493 1204 2898 4593 493 Size: 711 1694 1695 2702 Fragments arranged by size: 2702 1695 1694 711 BsaBI GATnn'nnATC Cuts at: 6796 6796 Size: 6802 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3114 3766 4609 4991 Size: 53 83 186 936 1581 652 843 382 Cuts at: 4991 5626 275 Size: 635 1451 Fragments arranged by size: 1581 1451 936 843 652 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2568 3612 5235 5633 5907 6054 6465 2568 Size: 1044 1623 398 274 147 411 2905 Fragments arranged by size: 2905 1623 1044 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6577 Fragments arranged by size: 6577 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 2408 1186 Size: 1219 3 5580 Fragments arranged by size: 5580 1219 3 BseYI C'CCAG_C Cuts at: 2281 6005 6501 2281 Size: 3724 496 2582 Fragments arranged by size: 3724 2582 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2803 4097 1163 Size: 573 1067 1294 3868 Fragments arranged by size: 3868 1294 1067 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2146 2192 3669 4252 5013 6041 Size: 275 186 145 46 1477 583 761 1028 Cuts at: 6041 1540 Size: 2301 Fragments arranged by size: 2301 1477 1028 761 583 275 186 145 46 BsiHKAI G_wGCw'C Cuts at: 2415 2619 2704 3657 4863 4948 6019 2415 Size: 204 85 953 1206 85 1071 3198 Fragments arranged by size: 3198 1206 1071 953 204 85 85 BsmI GAATG_Cn' Cuts at: 4313 4406 4313 Size: 93 6709 BsmBI CGTCTCn'nnnn_ Cuts at: 3656 3656 Size: 6802 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2215 2415 2619 2697 2704 3212 Size: 399 352 73 200 204 78 7 508 Cuts at: 3212 3657 3971 4121 4863 4948 6019 1391 Size: 445 314 150 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 508 445 399 352 314 204 200 150 85 78 73 7 BspEI T'CCGG_A Cuts at: 6465 6465 Size: 6802 BspHI T'CATG_A Cuts at: 2332 2607 3523 4691 6421 2332 Size: 275 916 1168 1730 2713 Fragments arranged by size: 2713 1730 1168 916 275 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2119 2265 3157 3412 4157 844 Size: 1275 146 892 255 745 3489 Fragments arranged by size: 3489 1275 892 745 255 146 BsrBI CCG'CTC Cuts at: 1394 1823 2311 3159 4689 1394 Size: 429 488 848 1530 3507 Fragments arranged by size: 3507 1530 848 488 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5297 5471 65 Size: 1183 4049 174 1396 Fragments arranged by size: 4049 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2165 2286 3612 5446 5633 1700 Size: 110 355 121 1326 1834 187 2869 Fragments arranged by size: 2869 1834 1326 355 187 121 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6802 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6802 BssSI C'ACGA_G Cuts at: 4862 5874 4862 Size: 1012 5790 BstAPI GCAn_nnn'nTGC Cuts at: 794 2235 3446 794 Size: 1441 1211 4150 Fragments arranged by size: 4150 1441 1211 BstBI TT'CG_AA Cuts at: 5606 5606 Size: 6802 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6802 BstXI CCAn_nnnn'nTGG Cuts at: 3428 3428 Size: 6802 BstYI r'GATC_y Cuts at: 1972 3332 4132 4499 4884 4901 6353 6439 6451 Size: 1360 800 367 385 17 1452 86 12 Cuts at: 6451 6797 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1360 800 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2932 2932 Size: 6802 BtgI C'CryG_G Cuts at: 513 1065 2004 2053 513 Size: 552 939 49 5262 Fragments arranged by size: 5262 939 552 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6063 Fragments arranged by size: 6063 683 56 BtsI GCAGTG_nn' Cuts at: 2415 4318 5112 5132 2415 Size: 1903 794 20 4085 Fragments arranged by size: 4085 1903 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6802 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6767 DraI TTT'AAA Cuts at: 4106 4106 Size: 6802 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5809 809 Size: 507 4493 1802 Fragments arranged by size: 4493 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2199 2601 Size: 54 1425 50 275 18 303 66 402 Cuts at: 2601 3309 4249 5138 6767 8 Size: 708 940 889 1629 43 Fragments arranged by size: 1629 1425 940 889 708 402 303 275 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4249 1537 Size: 275 2437 4090 Fragments arranged by size: 4090 2437 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2343 3077 3166 3867 3913 4082 4732 1353 Size: 990 734 89 701 46 169 650 3423 Fragments arranged by size: 3423 990 734 701 650 169 89 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5389 5763 5909 387 Size: 1064 138 3800 374 146 1280 Fragments arranged by size: 3800 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2702 2702 Size: 6802 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2781 2879 3207 3938 4865 5462 Size: 393 437 712 98 328 731 927 597 Cuts at: 5462 6249 6517 1239 Size: 787 268 1524 Fragments arranged by size: 1524 927 787 731 712 597 437 393 328 268 98 EcoNI CCTnn'n_nnAGG Cuts at: 2931 3940 2931 Size: 1009 5793 EcoO109I rG'GnC_Cy Cuts at: 2693 2814 3016 2693 Size: 121 202 6479 Fragments arranged by size: 6479 202 121 EcoRI G'AATT_C Cuts at: 4111 4489 4111 Size: 378 6424 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2448 2801 2448 Size: 353 6449 FspI TGC'GCA Cuts at: 5308 5308 Size: 6802 HaeII r_GCGC'y Cuts at: 1678 3117 5949 1678 Size: 1439 2832 2531 Fragments arranged by size: 2832 2531 1439 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2202 2234 2505 2537 2740 Size: 32 135 32 630 32 271 32 203 Cuts at: 2740 2772 2899 2931 3467 3499 3512 3544 5443 Size: 32 127 32 536 32 13 32 1899 Cuts at: 5443 5475 5517 5549 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1899 630 536 271 203 135 127 42 32 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 3472 3904 4146 4392 669 Size: 988 281 1534 432 242 246 3079 Fragments arranged by size: 3079 1534 988 432 281 246 242 HindIII A'AGCT_T Cuts at: 748 748 Size: 6802 HpaI GTT'AAC Cuts at: 3472 4392 3472 Size: 920 5882 KasI G'GCGC_C Cuts at: 3113 3113 Size: 6802 KpnI G_GTAC'C Cuts at: 4127 4127 Size: 6802 MfeI C'AATT_G Cuts at: 4401 4401 Size: 6802 MluI A'CGCG_T Cuts at: 6678 6678 Size: 6802 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 3787 5916 6100 1305 Size: 834 1648 2129 184 2007 Fragments arranged by size: 2129 2007 1648 834 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2603 3311 10 Size: 54 1425 343 369 402 708 3501 Fragments arranged by size: 3501 1425 708 402 369 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2523 4760 5119 5278 518 Size: 648 1357 2237 359 159 2042 Fragments arranged by size: 2237 2042 1357 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3360 4218 4895 6043 6288 1606 Size: 1754 858 677 1148 245 2120 Fragments arranged by size: 2120 1754 1148 858 677 245 NarI GG'CG_CC Cuts at: 3114 3114 Size: 6802 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5311 Fragments arranged by size: 5311 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6802 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6802 NotI GC'GGCC_GC Cuts at: 4249 4249 Size: 6802 NspI r_CATG'y Cuts at: 1132 1229 2677 4160 5705 1132 Size: 97 1448 1483 1545 2229 Fragments arranged by size: 2229 1545 1483 1448 97 PasI CC'CwG_GG Cuts at: 1348 2690 1348 Size: 1342 5460 PciI A'CATG_T Cuts at: 5701 5701 Size: 6802 PflMI CCAn_nnn'nTGG Cuts at: 1108 3822 1108 Size: 2714 4088 PfoI T'CCnGG_A Cuts at: 2085 2649 3095 2085 Size: 564 446 5792 Fragments arranged by size: 5792 564 446 PmeI GTTT'AAAC Cuts at: 4106 4106 Size: 6802 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4871 4903 6384 6416 4871 Size: 32 1481 32 5257 Fragments arranged by size: 5257 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2814 3016 2814 Size: 202 6600 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6802 PsiI TTA'TAA Cuts at: 2463 4372 2463 Size: 1909 4893 PspOMI G'GGCC_C Cuts at: 2138 2693 2138 Size: 555 6247 PspXI vC'TCGA_Gb Cuts at: 2208 2208 Size: 6802 PstI C_TGCA'G Cuts at: 830 2130 3587 4154 830 Size: 1300 1457 567 3478 Fragments arranged by size: 3478 1457 1300 567 PvuI CG_AT'CG Cuts at: 2001 2192 3669 2001 Size: 191 1477 5134 Fragments arranged by size: 5134 1477 191 SacI G_AGCT'C Cuts at: 2704 2704 Size: 6802 SalI G'TCGA_C Cuts at: 1936 3902 4144 1936 Size: 1966 242 4594 Fragments arranged by size: 4594 1966 242 SapI GCTCTTCn'nnn_ Cuts at: 3077 3166 3077 Size: 89 6713 SbfI CC_TGCA'GG Cuts at: 4154 4154 Size: 6802 ScaI AGT'ACT Cuts at: 2234 5050 6762 2234 Size: 2816 1712 2274 Fragments arranged by size: 2816 2274 1712 SfcI C'TryA_G Cuts at: 826 1045 2126 3232 3583 3708 4150 5285 5966 Size: 219 1081 1106 351 125 442 1135 681 Cuts at: 5966 6157 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1106 1081 681 442 351 219 191 125 SfoI GGC'GCC Cuts at: 3115 3115 Size: 6802 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6802 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6802 SmaI CCC'GGG Cuts at: 1872 2216 4129 1872 Size: 344 1913 4545 Fragments arranged by size: 4545 1913 344 SmlI C'TyrA_G Cuts at: 820 1017 1943 2208 4906 5807 6069 820 Size: 197 926 265 2698 901 262 1553 Fragments arranged by size: 2698 1553 926 901 265 262 197 SnaBI TAC'GTA Cuts at: 493 4593 493 Size: 4100 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6802 SphI G_CATG'C Cuts at: 1132 4160 1132 Size: 3028 3774 SspI AAT'ATT Cuts at: 5 52 2327 4726 5 Size: 47 2275 2399 2081 Fragments arranged by size: 2399 2275 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6802 StyI C'CwwG_G Cuts at: 513 1065 2004 2943 3109 3383 3994 4241 513 Size: 552 939 939 166 274 611 247 3074 Fragments arranged by size: 3074 939 939 611 552 274 247 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4829 4846 4999 5184 5629 325 Size: 1255 3249 17 153 185 445 1498 Fragments arranged by size: 3249 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2232 5048 6760 Size: 274 80 33 51 1443 255 2816 1712 Cuts at: 6760 96 Size: 138 Fragments arranged by size: 2816 1712 1443 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2812 2837 2844 2869 6762 6794 2812 Size: 25 7 25 3893 32 2820 Fragments arranged by size: 3893 2820 32 25 25 7 Tth111I GACn'n_nGTC Cuts at: 1316 2636 1316 Size: 1320 5482 XbaI T'CTAG_A Cuts at: 4138 4138 Size: 6802 XhoI C'TCGA_G Cuts at: 1943 2208 1943 Size: 265 6537 XmaI C'CCGG_G Cuts at: 1870 2214 4127 1870 Size: 344 1913 4545 Fragments arranged by size: 4545 1913 344 XmnI GAAnn'nnTTC Cuts at: 4931 4931 Size: 6802 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4610 276 Size: 53 83 186 936 3076 2468 Fragments arranged by size: 3076 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI