################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 225 GTCTAC GT^[AC][GT]AC AciI 498 GCGG CCGC(-3/-1) AfaI 950 GTAC GT^AC AflIII 816 ACATGT A^C[GA][CT]GT AgeI 920 ACCGGT A^CCGGT AlwI 666 GATCC GGATC(4/5) BanII 963 GGGCTC G[GA]GC[CT]^C BbsI 888 GAAGAC GAAGAC(2/6) BbvCI 717 CCTCAGC CCTCAGC(-5/-2) BbvI 501 GCTGC GCAGC(8/12) BcgI 457 CGAAGCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 250 CTAG C^TAG BpuAI 888 GAAGAC GAAGAC(2/6) BsaAI 902 TACGTG [CT]AC^GT[GA] BsaWI 920 ACCGGT [AT]^CCGG[AT] BspLU11I 816 ACATGT A^CATGT BsrDI 279 CATTGC GCAATG(2/0) BsrFI 920 ACCGGT [GA]^CCGG[CT] BsrI 478 CCAGT ACTGG(1/-1) Cfr10I 920 ACCGGT [GA]^CCGG[CT] DsaI 356 CCATGG C^C[GA][CT]GG HapII 921 CCGG C^CGG HincII 586 GTTAAC GT[CT]^[GA]AC HindII 586 GTTAAC GT[CT]^[GA]AC HindIII 459 AAGCTT A^AGCTT HpaI 586 GTTAAC GTT^AAC HpaII 921 CCGG C^CGG MaeI 250 CTAG C^TAG MseI 587 TTAA T^TAA MspI 921 CCGG C^CGG NcoI 356 CCATGG C^CATGG NdeI 319 CATATG CA^TATG NlaIV 730 GGCTCC GG[ACGT]^[ACGT]CC NspI 816 ACATGT [GA]CATG^[CT] PflMI 254 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 920 ACCGGT A^CCGGT RsaI 950 GTAC GT^AC SspI 805 AATATT AAT^ATT Tru9I 587 TTAA T^TAA TseI 501 GCTGC G^C[AT]GC Van91I 254 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 836 CCAATCTTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AluI 342 AGCT AG^CT 460 AGCT BalI 377 TGGCCA TGG^CCA 833 TGGCCA BstXI 614 CCATTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 789 CCATCATTTTGG Cac8I 201 GCTGGC GC[ACGT]^[ACGT]GC 831 GCTGGC Cfr13I 246 GGCCC G^G[ACGT]CC 714 GGCCC DpnII 367 GATC ^GATC 666 GATC DraII 245 AGGCCCT [GA]G^G[ACGT]CC[CT] 713 AGGCCCT EcoO109I 245 AGGCCCT [GA]G^G[ACGT]CC[CT] 713 AGGCCCT Fnu4HI 498 GCGGC GC^[ACGT]GC 501 GCTGC HinfI 3 GAGTC G^A[ACGT]TC 337 GAGTC HphI 299 TCACC GGTGA(8/7) 452 TCACC ItaI 498 GCGGC GC^[ACGT]GC 501 GCTGC MaeII 162 ACGT A^CGT 903 ACGT MboI 367 GATC ^GATC 666 GATC MluNI 377 TGGCCA TGG^CCA 833 TGGCCA MscI 377 TGGCCA TGG^CCA 833 TGGCCA NdeII 367 GATC ^GATC 666 GATC PleI 3 GAGTC GAGTC(4/5) 337 GAGTC PstI 32 CTGCAG CTGCA^G 116 CTGCAG Sau3AI 367 GATC ^GATC 666 GATC Sau96I 246 GGCCC G^G[ACGT]CC 714 GGCCC SfcI 32 CTGCAG C^T[GA][CT]AG 116 CTGCAG Tsp45I 5 GTCAC ^GT[GC]AC 298 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI DdeI EaeI EcoRII EcoT14I FokI HaeIII MaeIII MboII MnlI MslI MvaI MwoI NlaIII ScrFI SfaNI StyI TspRI