(Circular) (Six-base) MAPSORT of: pFN21AE3446.seq Check: 910 from: 1 to: 5866 pFN21AE3446 5866 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2373 3209 1937 Size: 436 836 4594 Fragments arranged by size: 4594 836 436 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2513 2527 3929 5313 5581 1632 Size: 881 14 1402 1384 268 1917 Fragments arranged by size: 1917 1402 1384 881 268 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 4765 5742 1203 Size: 3562 977 1327 Fragments arranged by size: 3562 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 2363 1166 Size: 1197 4669 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2548 1184 Size: 1364 4502 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2405 2437 2813 2845 2405 Size: 32 376 32 5426 Fragments arranged by size: 5426 376 32 32 AlwNI CAG_nnn'CTG Cuts at: 2219 2550 3609 5181 2219 Size: 331 1059 1572 2904 Fragments arranged by size: 2904 1572 1059 331 ApaI G_GGCC'C Cuts at: 3083 3083 Size: 5866 ApaLI G'TGCA_C Cuts at: 3923 5079 3923 Size: 1156 4710 ApoI r'AATT_y Cuts at: 1493 3175 3401 3553 5662 1493 Size: 1682 226 152 2109 1697 Fragments arranged by size: 2109 1697 1682 226 152 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 3191 1870 Size: 73 1248 4545 Fragments arranged by size: 4545 1248 73 BamHI G'GATC_C Cuts at: 2757 3196 3563 2757 Size: 439 367 5060 Fragments arranged by size: 5060 439 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2013 3187 4642 618 Size: 325 217 627 226 1174 1455 1842 Fragments arranged by size: 1842 1455 1174 627 325 226 217 BanII G_rGCy'C Cuts at: 1391 2997 3068 3083 3185 1391 Size: 1606 71 15 102 4072 Fragments arranged by size: 4072 1606 102 71 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4895 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2971 3005 4057 4091 1903 Size: 34 1034 34 1052 34 3678 Fragments arranged by size: 3678 1052 1034 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3758 4968 3758 Size: 1210 4656 BclI T'GATC_A Cuts at: 1548 2232 2928 1548 Size: 684 696 4486 Fragments arranged by size: 4486 696 684 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4477 136 Size: 107 122 71 631 605 2805 1525 Fragments arranged by size: 2805 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2418 5861 2418 Size: 3443 2423 BlpI GC'TnA_GC Cuts at: 2626 3283 2626 Size: 657 5209 Bme1580I G_kGCm'C Cuts at: 1790 2792 3083 3927 5083 1790 Size: 1002 291 844 1156 2573 Fragments arranged by size: 2573 1156 1002 844 291 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3597 4555 442 Size: 416 53 83 399 24 2180 958 1753 Fragments arranged by size: 2180 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2609 2662 2878 2927 3012 3158 4526 Size: 893 477 53 216 49 85 146 1368 Cuts at: 4526 1239 Size: 2579 Fragments arranged by size: 2579 1368 893 477 216 146 85 53 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2156 3991 4856 5154 2156 Size: 1835 865 298 2868 Fragments arranged by size: 2868 1835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4529 882 Size: 617 3030 2219 Fragments arranged by size: 3030 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 2762 5860 2762 Size: 3098 2768 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3673 4055 4690 275 Size: 53 83 186 936 2140 382 635 1451 Fragments arranged by size: 2140 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2562 3050 4299 4697 4971 5118 5529 2562 Size: 488 1249 398 274 147 411 2899 Fragments arranged by size: 2899 1249 488 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2405 2435 2876 2903 2906 Size: 30 165 30 1002 30 441 27 3 Cuts at: 2906 2933 3131 3161 1178 Size: 27 198 30 3883 Fragments arranged by size: 3883 1002 441 198 165 30 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5866 BseYI C'CCAG_C Cuts at: 2741 5069 5565 2741 Size: 2328 496 3042 Fragments arranged by size: 3042 2328 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2762 1163 Size: 573 1026 4267 Fragments arranged by size: 4267 1026 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2316 2997 3927 4012 5083 2316 Size: 681 930 85 1071 3099 Fragments arranged by size: 3099 1071 930 681 85 BsmI GAATG_Cn' Cuts at: 3377 3470 3377 Size: 93 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2316 2792 2997 3068 3083 3185 3927 Size: 399 526 476 205 71 15 102 742 Cuts at: 3927 4012 5083 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 476 399 205 102 85 71 15 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 3755 5485 3755 Size: 1730 4136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3221 844 Size: 2377 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2814 3753 1394 Size: 429 991 939 3507 Fragments arranged by size: 3507 991 939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2079 2143 4361 4535 65 Size: 1183 831 64 2218 174 1396 Fragments arranged by size: 2218 1396 1183 831 174 64 BsrFI r'CCGG_y Cuts at: 1700 1810 2098 2304 2667 4510 4697 1700 Size: 110 288 206 363 1843 187 2869 Fragments arranged by size: 2869 1843 363 288 206 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5866 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 2983 3926 4938 2983 Size: 943 1012 3911 Fragments arranged by size: 3911 1012 943 BstAPI GCAn_nnn'nTGC Cuts at: 794 2020 794 Size: 1226 4640 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5866 BstYI r'GATC_y Cuts at: 1972 2418 2757 3196 3563 3948 3965 5417 5503 Size: 446 339 439 367 385 17 1452 86 Cuts at: 5503 5515 5861 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 446 439 385 367 346 339 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2877 3059 513 Size: 552 1812 182 3320 Fragments arranged by size: 3320 1812 552 182 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 3382 4176 4196 3382 Size: 794 20 5052 Fragments arranged by size: 5052 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4873 809 Size: 507 3557 1802 Fragments arranged by size: 3557 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2712 3042 3313 Size: 54 1425 50 275 18 882 330 271 Cuts at: 3313 4202 5831 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 882 330 275 271 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2321 3796 1353 Size: 968 1475 3423 Fragments arranged by size: 3423 1475 968 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4453 4827 4973 387 Size: 1064 138 2864 374 146 1280 Fragments arranged by size: 2864 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2995 2995 Size: 5866 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2513 2527 2609 2662 2878 2927 Size: 393 500 381 14 82 53 216 49 Cuts at: 2927 3012 3158 3929 4526 5313 5581 1239 Size: 85 146 771 597 787 268 1524 Fragments arranged by size: 1524 787 771 597 500 393 381 268 216 146 85 82 53 49 14 EcoO109I rG'GnC_Cy Cuts at: 3004 3004 Size: 5866 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 FspI TGC'GCA Cuts at: 4372 4372 Size: 5866 HaeII r_GCGC'y Cuts at: 1678 3027 5013 1678 Size: 1349 1986 2531 Fragments arranged by size: 2531 1986 1349 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4507 4539 4581 4613 1373 Size: 32 135 32 2935 32 42 32 2626 Fragments arranged by size: 2935 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3210 3456 669 Size: 988 281 1272 246 3079 Fragments arranged by size: 3079 1272 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 2425 3465 2425 Size: 1040 4826 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4980 5164 1305 Size: 3675 184 2007 Fragments arranged by size: 3675 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2714 3044 10 Size: 54 1425 343 882 330 2832 Fragments arranged by size: 2832 1425 882 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 2705 2708 2855 3824 4183 4342 Size: 648 1197 342 3 147 969 359 159 Cuts at: 4342 518 Size: 2042 Fragments arranged by size: 2042 1197 969 648 359 342 159 147 3 MspA1I CmG'CkG Cuts at: 1606 2937 3282 3959 5107 5352 1606 Size: 1331 345 677 1148 245 2120 Fragments arranged by size: 2120 1331 1148 677 345 245 NaeI GCC'GGC Cuts at: 2306 2306 Size: 5866 NcoI C'CATG_G Cuts at: 513 1065 3059 513 Size: 552 1994 3320 Fragments arranged by size: 3320 1994 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NgoMIV G'CCGG_C Cuts at: 2304 2304 Size: 5866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 2675 2869 3224 4769 1132 Size: 97 1446 194 355 1545 2229 Fragments arranged by size: 2229 1545 1446 355 194 97 PasI CC'CwG_GG Cuts at: 1348 2605 1348 Size: 1257 4609 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5866 PfoI T'CCnGG_A Cuts at: 2177 2586 2177 Size: 409 5457 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2405 2437 3935 3967 5448 5480 2405 Size: 32 1498 32 1481 32 2791 Fragments arranged by size: 2791 1498 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3004 3004 Size: 5866 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PspOMI G'GGCC_C Cuts at: 3079 3079 Size: 5866 PstI C_TGCA'G Cuts at: 830 2025 3218 830 Size: 1195 1193 3478 Fragments arranged by size: 3478 1195 1193 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 SacI G_AGCT'C Cuts at: 2997 2997 Size: 5866 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SanDI GG'GwC_CC Cuts at: 3004 3004 Size: 5866 SapI GCTCTTCn'nnn_ Cuts at: 2321 2321 Size: 5866 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SfcI C'TryA_G Cuts at: 826 1045 2021 3214 4349 5030 5221 826 Size: 219 976 1193 1135 681 191 1471 Fragments arranged by size: 1471 1193 1135 976 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 2098 1700 Size: 398 5468 SmaI CCC'GGG Cuts at: 1872 3193 1872 Size: 1321 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2171 3970 4871 5133 820 Size: 197 926 228 1799 901 262 1553 Fragments arranged by size: 1799 1553 926 901 262 228 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 2675 2869 3224 1132 Size: 1543 194 355 3774 Fragments arranged by size: 3774 1543 355 194 SspI AAT'ATT Cuts at: 5 52 3790 5 Size: 47 3738 2081 Fragments arranged by size: 3738 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5866 StyI C'CwwG_G Cuts at: 513 1065 2085 3059 3305 513 Size: 552 1020 974 246 3074 Fragments arranged by size: 3074 1020 974 552 246 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3893 3910 4063 4248 4693 325 Size: 1255 2313 17 153 185 445 1498 Fragments arranged by size: 2313 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2883 4112 5824 Size: 274 80 33 51 1443 906 1229 1712 Cuts at: 5824 96 Size: 138 Fragments arranged by size: 1712 1443 1229 906 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2159 2191 5826 5858 2159 Size: 32 3635 32 2167 Fragments arranged by size: 3635 2167 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3114 1316 Size: 1798 4068 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5866 XmaI C'CCGG_G Cuts at: 1870 3191 1870 Size: 1321 4545 XmnI GAAnn'nnTTC Cuts at: 3995 3995 Size: 5866 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI