################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BseAI BsiHKAI BsiWI BsmFI BsmI BspDI BspEI BspLU11I BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI TfiI Tru9I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AcyI 710 GGCGCC G[GA]^CG[CT]C AflIII 746 ACGTGT A^C[GA][CT]GT AluI 496 AGCT AG^CT Aor51HI 605 AGCGCT AGC^GCT Asp700I 226 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 472 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 290 TGCGCA TGC^GCA BanI 710 GGCGCC G^G[CT][GA]CC BanII 723 GAGCCC G[GA]GC[CT]^C BbeI 710 GGCGCC GGCGC^C BbsI 282 GAAGAC GAAGAC(2/6) BlpI 33 GCTGAGC GC^T[ACGT]AGC BmyI 723 GAGCCC G[AGT]GC[ACT]^C Bpu1102I 33 GCTGAGC GC^T[ACGT]AGC BpuAI 282 GAAGAC GAAGAC(2/6) BsaHI 710 GGCGCC G[GA]^CG[CT]C BsaWI 240 ACCGGA [AT]^CCGG[AT] BsiEI 717 CGGCCG CG[GA][CT]^CG BsmBI 658 CGTCTC CGTCTC(1/5) Bsp1286I 723 GAGCCC G[AGT]GC[ACT]^C Bsp1407I 101 TGTACA T^GTACA BspHI 479 TCATGA T^CATGA BsrGI 101 TGTACA T^GTACA BsrI 95 ACTGG ACTGG(1/-1) BssSI 328 CTCGTG CACGAG(-5/-1) BstNI 66 CCTGG CC^[AT]GG CelII 33 GCTGAGC GC^T[ACGT]AGC DpnII 246 GATC ^GATC EagI 717 CGGCCG C^GGCCG EarI 322 CTCTTC CTCTTC(1/4) EclXI 717 CGGCCG C^GGCCG Eco47III 605 AGCGCT AGC^GCT Eco52I 717 CGGCCG C^GGCCG EcoRII 66 CCTGG ^CC[AT]GG FspI 290 TGCGCA TGC^GCA Hin1I 710 GGCGCC G[GA]^CG[CT]C KasI 710 GGCGCC G^GCGCC Ksp632I 322 CTCTTC CTCTTC(1/4) KspI 714 CCGCGG CCGC^GG MaeII 746 ACGT A^CGT MboI 246 GATC ^GATC MslI 753 CATCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 714 CCGCGG C[AC]G^C[GT]G MvaI 66 CCTGG CC^[AT]GG NarI 710 GGCGCC GG^CGCC NcoI 311 CCATGG C^CATGG NdeII 246 GATC ^GATC PflFI 472 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 479 TCATGA T^CATGA SacII 714 CCGCGG CCGC^GG Sau3AI 246 GATC ^GATC SfoI 710 GGCGCC GGC^GCC SspBI 101 TGTACA T^GTACA Tsp509I 250 AATT ^AATT Tth111I 472 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 226 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AlwNI 663 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG 688 CAGTTCCTG AvaI 681 CTCGGG C^[CT]CG[GA]G 700 CCCGAG AvaII 380 GGACC G^G[AT]CC 795 GGACC BcnI 639 CCCGG CC^[GC]GG 792 CCCGG BpmI 459 CTCCAG CTGGAG(16/14) 738 CTCCAG BseRI 616 GAGGAG GAGGAG(10/8) 619 GAGGAG BsgI 160 CTGCAC GTGCAG(16/14) 802 CTGCAC BsmAI 397 GTCTC GTCTC(1/5) 659 GTCTC BsoBI 681 CTCGGG C^[CT]CG[GA]G 700 CCCGAG BspMI 800 ACCTGC ACCTGC(4/8) 193 GCAGGT BsrFI 166 GCCGGC [GA]^CCGG[CT] 574 GCCGGC BstF5I 366 CATCC GGATG(2/0) 753 CATCC Cfr10I 166 GCCGGC [GA]^CCGG[CT] 574 GCCGGC DsaI 311 CCATGG C^C[GA][CT]GG 714 CCGCGG EcoT14I 311 CCATGG C^C[AT][AT]GG 431 CCTTGG FokI 366 CATCC GGATG(9/13) 753 CATCC HaeII 605 AGCGCT [GA]GCGC^[CT] 710 GGCGCC HgaI 762 GACGC GACGC(5/10) 657 GCGTC HinfI 3 GAGTC G^A[ACGT]TC 179 GAGTC HphI 255 GGTGA GGTGA(8/7) 599 TCACC MaeIII 5 GTCAC ^GT[ACGT]AC 256 GTGAC NaeI 166 GCCGGC GCC^GGC 574 GCCGGC NciI 639 CCCGG CC^[GC]GG 792 CCCGG NgoMIV 166 GCCGGC G^CCGGC 574 GCCGGC PleI 3 GAGTC GAGTC(4/5) 179 GAGTC PstI 9 CTGCAG CTGCA^G 694 CTGCAG SfcI 9 CTGCAG C^T[GA][CT]AG 694 CTGCAG SspI 227 AATATT AAT^ATT 274 AATATT StyI 311 CCATGG C^C[AT][AT]GG 431 CCTTGG Tsp45I 5 GTCAC ^GT[GC]AC 256 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AccII AciI AfaI BbvI BsaJI BsiYI BslI BssKI BstUI Cac8I CfoI Cfr13I DdeI EaeI FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvnI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI TseI TspRI