(Circular) (Six-base) MAPSORT of: pFN21AE5333.seq Check: 3489 from: 1 to: 5530 pFN21AE5333 5530 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2814 2814 Size: 5530 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3340 278 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2873 1937 Size: 936 4594 Acc65I G'GTAC_C Cuts at: 2851 2851 Size: 5530 AclI AA'CG_TT Cuts at: 1563 3657 4030 1563 Size: 2094 373 3063 Fragments arranged by size: 3063 2094 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2306 3593 4977 5245 1632 Size: 674 1287 1384 268 1917 Fragments arranged by size: 1917 1384 1287 674 268 AfeI AGC'GCT Cuts at: 2612 2612 Size: 5530 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5333 AflIII A'CryG_T Cuts at: 1203 2751 4429 5406 1203 Size: 1548 1678 977 1327 Fragments arranged by size: 1678 1548 1327 977 AgeI A'CCGG_T Cuts at: 4361 4361 Size: 5530 AhdI GACnn_n'nnGTC Cuts at: 4259 4259 Size: 5530 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5530 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5530 AlwNI CAG_nnn'CTG Cuts at: 2673 2698 3273 4845 2673 Size: 25 575 1572 3358 Fragments arranged by size: 3358 1572 575 25 ApaLI G'TGCA_C Cuts at: 3587 4743 3587 Size: 1156 4374 ApoI r'AATT_y Cuts at: 1493 2839 3065 3217 5326 1493 Size: 1346 226 152 2109 1697 Fragments arranged by size: 2109 1697 1346 226 152 AseI AT'TA_AT Cuts at: 160 1032 4084 160 Size: 872 3052 1606 Fragments arranged by size: 3052 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 AvaI C'yCGr_G Cuts at: 1870 1943 2686 2705 2855 1870 Size: 73 743 19 150 4545 Fragments arranged by size: 4545 743 150 73 19 BamHI G'GATC_C Cuts at: 2860 3227 2860 Size: 367 5163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2715 2851 4306 618 Size: 325 217 627 928 136 1455 1842 Fragments arranged by size: 1842 1455 928 627 325 217 136 BanII G_rGCy'C Cuts at: 1391 2732 2849 1391 Size: 1341 117 4072 Fragments arranged by size: 4072 1341 117 BbeI G_GCGC'C Cuts at: 2719 2719 Size: 5530 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2294 928 Size: 971 395 4164 Fragments arranged by size: 4164 971 395 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3721 3755 1903 Size: 34 1784 34 3678 Fragments arranged by size: 3678 1784 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3422 4632 3422 Size: 1210 4320 BclI T'GATC_A Cuts at: 1548 1548 Size: 5530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4141 136 Size: 107 122 71 631 605 2469 1525 Fragments arranged by size: 2469 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5525 5525 Size: 5530 BlpI GC'TnA_GC Cuts at: 2039 2947 2039 Size: 908 4622 Bme1580I G_kGCm'C Cuts at: 1790 3591 4747 1790 Size: 1801 1156 2573 Fragments arranged by size: 2573 1801 1156 BmgBI CAC'GTC Cuts at: 1318 2752 1318 Size: 1434 4096 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3261 4219 442 Size: 416 53 83 399 24 1844 958 1753 Fragments arranged by size: 1844 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2449 2728 4190 1239 Size: 1210 279 1462 2579 Fragments arranged by size: 2579 1462 1210 279 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3655 4520 4818 3655 Size: 865 298 4367 Fragments arranged by size: 4367 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4193 882 Size: 617 2694 2219 Fragments arranged by size: 2694 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3321 493 Size: 711 2117 2702 Fragments arranged by size: 2702 2117 711 BsaBI GATnn'nnATC Cuts at: 5524 5524 Size: 5530 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2716 3337 3719 4354 Size: 53 83 186 936 1183 621 382 635 Cuts at: 4354 275 Size: 1451 Fragments arranged by size: 1451 1183 936 635 621 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2245 3963 4361 4635 4782 5193 2245 Size: 1718 398 274 147 411 2582 Fragments arranged by size: 2582 1718 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2615 2645 1178 Size: 30 165 30 1212 30 4063 Fragments arranged by size: 4063 1212 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2636 2639 1186 Size: 1450 3 4077 Fragments arranged by size: 4077 1450 3 BseYI C'CCAG_C Cuts at: 2495 4733 5229 2495 Size: 2238 496 2796 Fragments arranged by size: 2796 2238 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2792 1163 Size: 573 414 642 3901 Fragments arranged by size: 3901 642 573 414 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2725 2980 3741 4769 1540 Size: 275 186 724 255 761 1028 2301 Fragments arranged by size: 2301 1028 761 724 275 255 186 BsiHKAI G_wGCw'C Cuts at: 3591 3676 4747 3591 Size: 85 1071 4374 Fragments arranged by size: 4374 1071 85 BsmI GAATG_Cn' Cuts at: 3041 3134 3041 Size: 93 5437 BsmBI CGTCTCn'nnnn_ Cuts at: 2669 2669 Size: 5530 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2732 2849 3591 3676 4747 1391 Size: 399 942 117 742 85 1071 2174 Fragments arranged by size: 2174 1071 942 742 399 117 85 BspEI T'CCGG_A Cuts at: 5193 5193 Size: 5530 BspHI T'CATG_A Cuts at: 2484 3419 5149 2484 Size: 935 1730 2865 Fragments arranged by size: 2865 1730 935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2189 2814 2885 844 Size: 1345 625 71 3489 Fragments arranged by size: 3489 1345 625 71 BsrBI CCG'CTC Cuts at: 1394 1823 3417 1394 Size: 429 1594 3507 Fragments arranged by size: 3507 1594 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4025 4199 65 Size: 1183 2777 174 1396 Fragments arranged by size: 2777 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2171 2579 4174 4361 1700 Size: 110 361 408 1595 187 2869 Fragments arranged by size: 2869 1595 408 361 187 110 BsrGI T'GTAC_A Cuts at: 96 2106 96 Size: 2010 3520 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5530 BssSI C'ACGA_G Cuts at: 2333 3590 4602 2333 Size: 1257 1012 3261 Fragments arranged by size: 3261 1257 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5530 BstBI TT'CG_AA Cuts at: 4334 4334 Size: 5530 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5530 BstYI r'GATC_y Cuts at: 1972 2860 3227 3612 3629 5081 5167 5179 5525 Size: 888 367 385 17 1452 86 12 346 Cuts at: 5525 1972 Size: 1977 Fragments arranged by size: 1977 1452 888 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2316 2719 513 Size: 552 1251 403 3324 Fragments arranged by size: 3324 1251 552 403 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2065 487 Size: 683 56 839 3952 Fragments arranged by size: 3952 839 683 56 BtsI GCAGTG_nn' Cuts at: 2011 3046 3840 3860 2011 Size: 1035 794 20 3681 Fragments arranged by size: 3681 1035 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5495 DraI TTT'AAA Cuts at: 2834 2834 Size: 5530 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4537 809 Size: 507 3221 1802 Fragments arranged by size: 3221 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 2365 2722 Size: 54 1425 50 275 18 471 64 357 Cuts at: 2722 2977 3866 5495 8 Size: 255 889 1629 43 Fragments arranged by size: 1629 1425 889 471 357 275 255 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2722 2977 1537 Size: 275 910 255 4090 Fragments arranged by size: 4090 910 275 255 EarI CTCTTCn'nnn_ Cuts at: 1353 2333 3460 1353 Size: 980 1127 3423 Fragments arranged by size: 3423 1127 980 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2363 4117 4491 4637 387 Size: 1064 138 774 1754 374 146 1280 Fragments arranged by size: 1754 1280 1064 774 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2306 2449 2728 3593 4190 4977 5245 Size: 393 674 143 279 865 597 787 268 Cuts at: 5245 1239 Size: 1524 Fragments arranged by size: 1524 865 787 674 597 393 279 268 143 EcoRI G'AATT_C Cuts at: 2839 3217 2839 Size: 378 5152 FspI TGC'GCA Cuts at: 2297 4036 2297 Size: 1739 3791 FspAI rTGC'GCAy Cuts at: 2297 2297 Size: 5530 HaeII r_GCGC'y Cuts at: 1678 2614 2719 4677 1678 Size: 936 105 1958 2531 Fragments arranged by size: 2531 1958 936 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2620 2652 4171 4203 4245 Size: 32 135 32 1048 32 1519 32 42 Cuts at: 4245 4277 1373 Size: 32 2626 Fragments arranged by size: 2626 1519 1048 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2874 3120 669 Size: 988 281 936 246 3079 Fragments arranged by size: 3079 988 936 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5530 HpaI GTT'AAC Cuts at: 3120 3120 Size: 5530 KasI G'GCGC_C Cuts at: 2715 2715 Size: 5530 KpnI G_GTAC'C Cuts at: 2855 2855 Size: 5530 MfeI C'AATT_G Cuts at: 3129 3129 Size: 5530 MluI A'CGCG_T Cuts at: 5406 5406 Size: 5530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2703 2760 4644 4828 1305 Size: 1398 57 1884 184 2007 Fragments arranged by size: 2007 1884 1398 184 57 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3708 Fragments arranged by size: 3708 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2762 3488 3847 4006 518 Size: 648 1596 726 359 159 2042 Fragments arranged by size: 2042 1596 726 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2721 2946 3623 4771 5016 1606 Size: 1115 225 677 1148 245 2120 Fragments arranged by size: 2120 1148 1115 677 245 225 NaeI GCC'GGC Cuts at: 2173 2581 2173 Size: 408 5122 NarI GG'CG_CC Cuts at: 2716 2716 Size: 5530 NcoI C'CATG_G Cuts at: 513 1065 2316 513 Size: 552 1251 3727 Fragments arranged by size: 3727 1251 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5530 NgoMIV G'CCGG_C Cuts at: 2171 2579 2171 Size: 408 5122 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5530 NotI GC'GGCC_GC Cuts at: 2977 2977 Size: 5530 NspI r_CATG'y Cuts at: 1132 1229 2888 4433 1132 Size: 97 1659 1545 2229 Fragments arranged by size: 2229 1659 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5530 PciI A'CATG_T Cuts at: 4429 4429 Size: 5530 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5530 PmeI GTTT'AAAC Cuts at: 2834 2834 Size: 5530 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3599 3631 5112 5144 3599 Size: 32 1481 32 3985 Fragments arranged by size: 3985 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5530 PsiI TTA'TAA Cuts at: 3100 3100 Size: 5530 PstI C_TGCA'G Cuts at: 830 2018 2703 2882 830 Size: 1188 685 179 3478 Fragments arranged by size: 3478 1188 685 179 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5530 SacII CC_GC'GG Cuts at: 2722 2722 Size: 5530 SalI G'TCGA_C Cuts at: 1936 2872 1936 Size: 936 4594 SbfI CC_TGCA'GG Cuts at: 2882 2882 Size: 5530 ScaI AGT'ACT Cuts at: 3778 5490 3778 Size: 1712 3818 SfcI C'TryA_G Cuts at: 826 1045 2014 2699 2878 4013 4694 4885 826 Size: 219 969 685 179 1135 681 191 1471 Fragments arranged by size: 1471 1135 969 685 681 219 191 179 SfoI GGC'GCC Cuts at: 2717 2717 Size: 5530 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5530 SmaI CCC'GGG Cuts at: 1872 2857 1872 Size: 985 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3634 4535 4797 820 Size: 197 926 1691 901 262 1553 Fragments arranged by size: 1691 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3321 493 Size: 2828 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5530 SphI G_CATG'C Cuts at: 1132 2888 1132 Size: 1756 3774 SspI AAT'ATT Cuts at: 5 52 2234 2281 3454 5 Size: 47 2182 47 1173 2081 Fragments arranged by size: 2182 2081 1173 47 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5530 StyI C'CwwG_G Cuts at: 513 1065 2316 2436 2969 513 Size: 552 1251 120 533 3074 Fragments arranged by size: 3074 1251 552 533 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3557 3574 3727 3912 4357 325 Size: 1255 1977 17 153 185 445 1498 Fragments arranged by size: 1977 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 3776 5488 Size: 274 80 33 51 1443 129 1670 1712 Cuts at: 5488 96 Size: 138 Fragments arranged by size: 1712 1670 1443 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5490 5522 5490 Size: 32 5498 Tth111I GACn'n_nGTC Cuts at: 1316 2480 1316 Size: 1164 4366 XbaI T'CTAG_A Cuts at: 2866 2866 Size: 5530 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5530 XmaI C'CCGG_G Cuts at: 1870 2855 1870 Size: 985 4545 XmnI GAAnn'nnTTC Cuts at: 2235 3659 2235 Size: 1424 4106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3338 276 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI