################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BmyI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HapII Hin1I HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AfaI 162 GTAC GT^AC AflIII 386 ACATGT A^C[GA][CT]GT AlwNI 214 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 100 CCTGAGG CC^T[ACGT]AGG Aor51HI 6 AGCGCT AGC^GCT Asp700I 197 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BfaI 119 CTAG C^TAG BglII 363 AGATCT A^GATCT BseRI 103 GAGGAG GAGGAG(10/8) BsgI 42 CTGCAC GTGCAG(16/14) BspLU11I 386 ACATGT A^CATGT BsrDI 378 CATTGC GCAATG(2/0) BssSI 110 CTCGTG CACGAG(-5/-1) BstYI 363 AGATCT [GA]^GATC[CT] Bsu36I 100 CCTGAGG CC^T[ACGT]AGG Cfr13I 455 GGCCC G^G[ACGT]CC DpnII 364 GATC ^GATC Eco47III 6 AGCGCT AGC^GCT Eco81I 100 CCTGAGG CC^T[ACGT]AGG HgaI 122 GCGTC GACGC(5/10) HincII 313 GTCAAC GT[CT]^[GA]AC HindII 313 GTCAAC GT[CT]^[GA]AC MaeI 119 CTAG C^TAG MaeIII 25 GTCAC ^GT[ACGT]AC MboI 364 GATC ^GATC MflI 363 AGATCT [GA]^GATC[CT] MslI 153 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 364 GATC ^GATC PaeR7I 69 CTCGAG C^TCGAG PleI 106 GAGTC GAGTC(4/5) PstI 206 CTGCAG CTGCA^G RsaI 162 GTAC GT^AC Sau3AI 364 GATC ^GATC Sau96I 455 GGCCC G^G[ACGT]CC SfcI 206 CTGCAG C^T[GA][CT]AG TaqI 70 TCGA T^CGA TfiI 197 GAATC G^A[AT]TC Tsp45I 25 GTCAC ^GT[GC]AC TthHB8I 70 TCGA T^CGA XhoI 69 CTCGAG C^TCGAG XhoII 363 AGATCT [GA]^GATC[CT] XmnI 197 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 155 CCGC CCGC(-3/-1) 251 GCGG AvaI 69 CTCGAG C^[CT]CG[GA]G 263 CCCGAG BlpI 211 GCTCAGC GC^T[ACGT]AGC 369 GCTGAGC Bpu1102I 211 GCTCAGC GC^T[ACGT]AGC 369 GCTGAGC BsiYI 244 CCAACAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 457 CCCATCACAGG BslI 244 CCAACAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 457 CCCATCACAGG BsoBI 69 CTCGAG C^[CT]CG[GA]G 263 CCCGAG BssKI 144 CCTGG ^CC[ACGT]GG 438 CCTGG BstNI 144 CCTGG CC^[AT]GG 438 CCTGG CelII 211 GCTCAGC GC^T[ACGT]AGC 369 GCTGAGC CfoI 7 GCGC GCG^C 141 GCGC EcoRII 144 CCTGG ^CC[AT]GG 438 CCTGG HaeII 6 AGCGCT [GA]GCGC^[CT] 140 AGCGCC HaeIII 39 GGCC GG^CC 455 GGCC HhaI 7 GCGC GCG^C 141 GCGC HinP1I 7 GCGC G^CGC 141 GCGC HinfI 106 GAGTC G^A[ACGT]TC 197 GAATC MboII 361 GAAGA GAAGA(8/7) 56 TCTTC MvaI 144 CCTGG CC^[AT]GG 438 CCTGG NspI 386 ACATGT [GA]CATG^[CT] 407 ACATGC ScrFI 144 CCTGG CC^[ACGT]GG 438 CCTGG SmlI 69 CTCGAG C^T[CT][GA]AG 184 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI BbvI BsmAI BstF5I Cac8I DdeI Fnu4HI FokI ItaI MnlI MwoI NlaIII SfaNI TseI