Restriction map for
pFN21AE5136
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AvrII
BamHI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr13I ClaI CpoI DraII
DraIII DrdI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HinfI HpaI HphI KpnI
Ksp632I MaeI MaeII MamI MboII
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tsp509I Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 30 GTCTAC GT^[AC][GT]AC
AcyI 45 GGCGCC G[GA]^CG[CT]C
AlwI 153 GATCC GGATC(4/5)
AlwNI 205 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 85 AGCGCT AGC^GCT
AspHI 258 GAGCTC G[AT]GC[AT]^C
AviII 225 TGCGCA TGC^GCA
BalI 62 TGGCCA TGG^CCA
BanII 258 GAGCTC G[GA]GC[CT]^C
BbeI 45 GGCGCC GGCGC^C
BbvI 73 GCAGC GCAGC(8/12)
BcgI 15 GCAAACTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 130 CCGGG CC^[GC]GG
BglI 64 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 45 GGCGCC G[GA]^CG[CT]C
BsiEI 127 CGGCCG CG[GA][CT]^CG
BsiHKAI 258 GAGCTC G[AT]GC[AT]^C
BspMI 245 ACCTGC ACCTGC(4/8)
BsrI 34 ACTGG ACTGG(1/-1)
BstNI 210 CCTGG CC^[AT]GG
BstYI 152 AGATCC [GA]^GATC[CT]
DpnII 153 GATC ^GATC
DraI 235 TTTAAA TTT^AAA
EagI 127 CGGCCG C^GGCCG
EclXI 127 CGGCCG C^GGCCG
Eco47III 85 AGCGCT AGC^GCT
Eco52I 127 CGGCCG C^GGCCG
EcoRII 210 CCTGG ^CC[AT]GG
EcoT14I 365 CCAAGG C^C[AT][AT]GG
FspI 225 TGCGCA TGC^GCA
HgaI 350 GCGTC GACGC(5/10)
Hin1I 45 GGCGCC G[GA]^CG[CT]C
KasI 45 GGCGCC G^GCGCC
KspI 393 CCGCGG CCGC^GG
MaeIII 352 GTCAC ^GT[ACGT]AC
MboI 153 GATC ^GATC
MflI 152 AGATCC [GA]^GATC[CT]
MluNI 62 TGGCCA TGG^CCA
MscI 62 TGGCCA TGG^CCA
MseI 236 TTAA T^TAA
MslI 354 CACTATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 210 CCTGG CC^[AT]GG
NarI 45 GGCGCC GG^CGCC
NciI 130 CCGGG CC^[GC]GG
NdeII 153 GATC ^GATC
NlaIII 360 CATG CATG^
PflMI 204 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 277 CTGCAG CTGCA^G
SacI 258 GAGCTC GAGCT^C
SacII 393 CCGCGG CCGC^GG
Sau3AI 153 GATC ^GATC
SfaNI 389 GCATC GCATC(5/9)
SfcI 277 CTGCAG C^T[GA][CT]AG
SfoI 45 GGCGCC GGC^GCC
SgrAI 96 CACCGGCG C[GA]^CCGG[CT]G
StuI 293 AGGCCT AGG^CCT
StyI 365 CCAAGG C^C[AT][AT]GG
Tru9I 236 TTAA T^TAA
TseI 73 GCAGC G^C[AT]GC
Tsp45I 352 GTCAC ^GT[GC]AC
TspRI 174 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 204 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 152 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AluI 259 AGCT AG^CT
377 AGCT
BanI 45 GGCGCC G^G[CT][GA]CC
133 GGCACC
BmyI 132 GGGCAC G[AGT]GC[ACT]^C
258 GAGCTC
BsiYI 91 CCGGCCACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
204 CCAGCGCCTGG
BslI 91 CCGGCCACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
204 CCAGCGCCTGG
Bsp1286I 132 GGGCAC G[AGT]GC[ACT]^C
258 GAGCTC
BsrFI 97 ACCGGC [GA]^CCGG[CT]
125 ACCGGC
BssKI 130 CCGGG ^CC[ACGT]GG
210 CCTGG
Cfr10I 97 ACCGGC [GA]^CCGG[CT]
125 ACCGGC
DdeI 11 CTAAG C^T[ACGT]AG
68 CTAAG
DsaI 137 CCGTGG C^C[GA][CT]GG
393 CCGCGG
FauI 77 CCCGC CCCGC(4/6)
383 CCCGC
MspA1I 263 CCGCTG C[AC]G^C[GT]G
393 CCGCGG
NlaIV 45 GGCGCC GG[ACGT]^[ACGT]CC
133 GGCACC
ScrFI 130 CCGGG CC^[ACGT]GG
210 CCTGG
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AccII AciI BsaJI BstF5I BstUI
Cac8I CfoI EaeI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MnlI MspI MvnI
MwoI