################################## # NO CUT ENZYMES ( 44 enzymes) # ################################## AclI AgeI Aor51HI AscI AviII BbeI BsiWI BssHII Bst1107I BstBI BstZ17I CpoI DrdI EagI EclXI Eco47III Eco52I FseI FspI HpaI KasI KspI MluI NarI NdeI NotI NruI NspV PacI PinAI PmeI Psp1406I PvuI RsrII SacII SbfI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AatII 2977 GACGTC GACGT^C Acc65I 1747 GGTACC G^GTACC AflII 6438 CTTAAG C^TTAAG AseI 5125 ATTAAT AT^TAAT AsnI 5125 ATTAAT AT^TAAT Asp718I 1747 GGTACC G^GTACC BciVI 3877 GGATAC GTATCC(6/5) BfrI 6438 CTTAAG C^TTAAG BglII 5769 AGATCT A^GATCT EcoT22I 2890 ATGCAT ATGCA^T KpnI 1747 GGTACC GGTAC^C MfeI 3934 CAATTG C^AATTG MunI 3934 CAATTG C^AATTG NheI 6426 GCTAGC G^CTAGC NsiI 2890 ATGCAT ATGCA^T PaeR7I 496 CTCGAG C^TCGAG PshAI 1179 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5125 ATTAAT AT^TAAT SalI 6016 GTCGAC G^TCGAC ScaI 5149 AGTACT AGT^ACT SfiI 7497 GGCCGTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 6659 CCCGGG CCC^GGG XhoI 496 CTCGAG C^TCGAG XmaCI 6659 CCCGGG C^CCGGG XmaI 6659 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 9180 CGCG CG^CG 9201 CGCG AhdI 406 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 8833 GACAGCGAGTC AspEI 406 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 8833 GACAGCGAGTC AvrII 3873 CCTAGG C^CTAGG 6444 CCTAGG BlnI 3873 CCTAGG C^CTAGG 6444 CCTAGG BsiEI 9137 CGGTCG CG[GA][CT]^CG 9315 CGACCG BspDI 2263 ATCGAT AT^CGAT 2545 ATCGAT BstEII 7253 GGTCACC G^GT[ACGT]ACC 9408 GGTCACC BstPI 7253 GGTCACC G^GT[ACGT]ACC 9408 GGTCACC BstUI 9180 CGCG CG^CG 9201 CGCG ClaI 2263 ATCGAT AT^CGAT 2545 ATCGAT DraI 2694 TTTAAA TTT^AAA 4290 TTTAAA Eam1105I 406 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 8833 GACAGCGAGTC EcoO65I 7253 GGTCACC G^GT[ACGT]ACC 9408 GGTCACC EcoRI 4541 GAATTC G^AATTC 5018 GAATTC EcoRV 1576 GATATC GAT^ATC 5010 GATATC MvnI 9180 CGCG CG^CG 9201 CGCG NaeI 7146 GCCGGC GCC^GGC 7549 GCCGGC NgoMIV 7146 GCCGGC G^CCGGC 7549 GCCGGC SspI 848 AATATT AAT^ATT 7693 AATATT XbaI 3622 TCTAGA T^CTAGA 8943 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 168 enzymes) # ######################################### AccI AccIII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI Asp700I AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI