Restriction map for
pFN21AB5943
(Linear) (Six-base) MAPSORT of: KIBB5943.seq Check: 9458 from: 1 to: 1524
KIBB5943.orf
With 182 enzymes: SgfI *
September 25, 2012 15:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 799 1524
Size: 725 74 725
Fragments arranged by size:
725 725 74
AgeI A'CCGG_T
Cuts at: 0 345 1524
Size: 345 1179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 74 1524
Size: 74 1450
AlwNI CAG_nnn'CTG
Cuts at: 0 586 1524
Size: 586 938
ApoI r'AATT_y
Cuts at: 0 91 638 850 1472 1524
Size: 91 547 212 622 52
Fragments arranged by size:
622 547 212 91 52
AvaI C'yCGr_G
Cuts at: 0 237 1524
Size: 237 1287
BamHI G'GATC_C
Cuts at: 0 3 1524
Size: 3 1521
BanI G'GyrC_C
Cuts at: 0 58 1524
Size: 58 1466
BanII G_rGCy'C
Cuts at: 0 31 200 302 1524
Size: 31 169 102 1222
Fragments arranged by size:
1222 169 102 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 102 427 560 787 896 1524
Size: 102 325 133 227 109 628
Fragments arranged by size:
628 325 227 133 109 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1306 1340 1524
Size: 1306 34 184
Fragments arranged by size:
1306 184 34
BfrBI ATG'CAT
Cuts at: 0 329 1524
Size: 329 1195
BglI GCCn_nnn'nGGC
Cuts at: 0 67 1524
Size: 67 1457
BglII A'GATC_T
Cuts at: 0 446 1524
Size: 446 1078
Bme1580I G_kGCm'C
Cuts at: 0 1312 1524
Size: 1312 212
BmrI ACTGGGnnnn_n'
Cuts at: 0 447 1524
Size: 447 1077
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 169 252 1524
Size: 169 83 1272
Fragments arranged by size:
1272 169 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1524
Size: 274 1250
Bpu10I CC'TnA_GC
Cuts at: 0 1159 1524
Size: 1159 365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 65 1524
Size: 45 20 1459
Fragments arranged by size:
1459 45 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 36 1361 1524
Size: 36 1325 163
Fragments arranged by size:
1325 163 36
BsaBI GATnn'nnATC
Cuts at: 0 738 1524
Size: 738 786
BsaWI w'CCGG_w
Cuts at: 0 345 969 1001 1524
Size: 345 624 32 523
Fragments arranged by size:
624 523 345 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 284 314 1524
Size: 284 30 1210
Fragments arranged by size:
1210 284 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 63 68 292 691 1524
Size: 63 5 224 399 833
Fragments arranged by size:
833 399 224 63 5
BsiHKAI G_wGCw'C
Cuts at: 0 200 302 1524
Size: 200 102 1222
Fragments arranged by size:
1222 200 102
BsmI GAATG_Cn'
Cuts at: 0 331 866 1524
Size: 331 535 658
Fragments arranged by size:
658 535 331
Bsp1286I G_dGCh'C
Cuts at: 0 31 200 302 1312 1524
Size: 31 169 102 1010 212
Fragments arranged by size:
1010 212 169 102 31
BspEI T'CCGG_A
Cuts at: 0 969 1001 1524
Size: 969 32 523
Fragments arranged by size:
969 523 32
BsrBI CCG'CTC
Cuts at: 0 1395 1524
Size: 1395 129
BsrFI r'CCGG_y
Cuts at: 0 345 1524
Size: 345 1179
BsrGI T'GTAC_A
Cuts at: 0 438 1524
Size: 438 1086
BssSI C'ACGA_G
Cuts at: 0 11 1524
Size: 11 1513
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1293 1524
Size: 1293 231
BstYI r'GATC_y
Cuts at: 0 3 446 1524
Size: 3 443 1078
Fragments arranged by size:
1078 443 3
BtgI C'CryG_G
Cuts at: 0 1507 1524
Size: 1507 17
DraI TTT'AAA
Cuts at: 0 482 1524
Size: 482 1042
EarI CTCTTCn'nnn_
Cuts at: 0 34 378 1064 1524
Size: 34 344 686 460
Fragments arranged by size:
686 460 344 34
EcoICRI GAG'CTC
Cuts at: 0 198 300 1524
Size: 198 102 1224
Fragments arranged by size:
1224 198 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 725 799 1524
Size: 274 451 74 725
Fragments arranged by size:
725 451 274 74
EcoO109I rG'GnC_Cy
Cuts at: 0 412 1524
Size: 412 1112
EcoRI G'AATT_C
Cuts at: 0 638 1524
Size: 638 886
EcoRV GAT'ATC
Cuts at: 0 736 1524
Size: 736 788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 494 1093 1524
Size: 494 599 431
Fragments arranged by size:
599 494 431
FspI TGC'GCA
Cuts at: 0 1481 1524
Size: 1481 43
HaeII r_GCGC'y
Cuts at: 0 1380 1524
Size: 1380 144
HincII GTy'rAC
Cuts at: 0 1145 1524
Size: 1145 379
MfeI C'AATT_G
Cuts at: 0 431 1524
Size: 431 1093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 1524
Size: 815 709
MslI CAynn'nnrTG
Cuts at: 0 619 1291 1524
Size: 619 672 233
Fragments arranged by size:
672 619 233
MspA1I CmG'CkG
Cuts at: 0 902 1259 1524
Size: 902 357 265
Fragments arranged by size:
902 357 265
NsiI A_TGCA'T
Cuts at: 0 331 1524
Size: 331 1193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 781 813 1524
Size: 781 32 711
Fragments arranged by size:
781 711 32
PvuII CAG'CTG
Cuts at: 0 902 1259 1524
Size: 902 357 265
Fragments arranged by size:
902 357 265
SacI G_AGCT'C
Cuts at: 0 200 302 1524
Size: 200 102 1222
Fragments arranged by size:
1222 200 102
SapI GCTCTTCn'nnn_
Cuts at: 0 1064 1524
Size: 1064 460
SmlI C'TyrA_G
Cuts at: 0 24 80 1524
Size: 24 56 1444
Fragments arranged by size:
1444 56 24
StyI C'CwwG_G
Cuts at: 0 656 1524
Size: 656 868
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 229 1524
Size: 229 1295
TatI w'GTAC_w
Cuts at: 0 276 438 1524
Size: 276 162 1086
Fragments arranged by size:
1086 276 162
Tth111I GACn'n_nGTC
Cuts at: 0 895 1524
Size: 895 629
XmnI GAAnn'nnTTC
Cuts at: 0 178 638 796 1524
Size: 178 460 158 728
Fragments arranged by size:
728 460 178 158
Enzymes that do cut:
AcuI AgeI AlfI AlwNI ApoI AvaI BamHI BanI
BanII BbsI BcgI BfrBI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrFI BsrGI
BssSI BstXI BstYI BtgI DraI EarI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII HincII MfeI
MmeI MslI MspA1I NsiI PpiI PvuII SacI SapI
SmlI StyI TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BciVI BclI BlpI BmgBI
BmtI BsaAI BsaHI BseYI BsgI BsiEI BsiWI BsmBI
BspHI BspMI BsrDI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EagI EciI EcoNI FseI FspAI Hin4I HindIII HpaI
KasI KpnI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI XbaI XcmI XhoI XmaI ZraI