(Linear) (Six-base) MAPSORT of: KIEE6758.seq Check: 7738 from: 1 to: 1749 KIEE6758.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AccI GT'mk_AC Cuts at: 0 520 1256 1749 Size: 520 736 493 Fragments arranged by size: 736 520 493 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 449 1012 1092 1283 1687 1749 Size: 97 352 563 80 191 404 62 Fragments arranged by size: 563 404 352 191 97 80 62 AflIII A'CryG_T Cuts at: 0 301 1749 Size: 301 1448 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1108 1140 1749 Size: 1108 32 609 Fragments arranged by size: 1108 609 32 AlwNI CAG_nnn'CTG Cuts at: 0 1503 1566 1749 Size: 1503 63 183 Fragments arranged by size: 1503 183 63 ApoI r'AATT_y Cuts at: 0 274 564 1611 1749 Size: 274 290 1047 138 Fragments arranged by size: 1047 290 274 138 AvrII C'CTAG_G Cuts at: 0 807 1749 Size: 807 942 BamHI G'GATC_C Cuts at: 0 768 1749 Size: 768 981 BanI G'GyrC_C Cuts at: 0 1374 1749 Size: 1374 375 BanII G_rGCy'C Cuts at: 0 334 1749 Size: 334 1415 BbeI G_GCGC'C Cuts at: 0 1378 1749 Size: 1378 371 BbsI GAAGACnn'nnnn_ Cuts at: 0 687 1749 Size: 687 1062 BbvCI CC'TCA_GC Cuts at: 0 1370 1749 Size: 1370 379 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1452 1486 1749 Size: 1452 34 263 Fragments arranged by size: 1452 263 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1257 1749 Size: 1257 492 BlpI GC'TnA_GC Cuts at: 0 247 1749 Size: 247 1502 BmrI ACTGGGnnnn_n' Cuts at: 0 346 924 1440 1749 Size: 346 578 516 309 Fragments arranged by size: 578 516 346 309 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 1135 1441 1457 1503 1749 Size: 155 980 306 16 46 246 Fragments arranged by size: 980 306 246 155 46 16 Bpu10I CC'TnA_GC Cuts at: 0 1370 1749 Size: 1370 379 BsaHI Gr'CG_yC Cuts at: 0 1375 1749 Size: 1375 374 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 331 361 1108 1138 1749 Size: 331 30 747 30 611 Fragments arranged by size: 747 611 331 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 67 117 471 894 937 1500 1749 Size: 67 50 354 423 43 563 249 Fragments arranged by size: 563 423 354 249 67 50 43 BseYI C'CCAG_C Cuts at: 0 889 1749 Size: 889 860 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 482 1128 1336 1553 1749 Size: 482 646 208 217 196 Fragments arranged by size: 646 482 217 208 196 BsiHKAI G_wGCw'C Cuts at: 0 779 816 1155 1749 Size: 779 37 339 594 Fragments arranged by size: 779 594 339 37 BsmI GAATG_Cn' Cuts at: 0 702 745 1351 1548 1749 Size: 702 43 606 197 201 Fragments arranged by size: 702 606 201 197 43 Bsp1286I G_dGCh'C Cuts at: 0 334 779 816 1155 1749 Size: 334 445 37 339 594 Fragments arranged by size: 594 445 339 334 37 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 532 1749 Size: 532 1217 BsrFI r'CCGG_y Cuts at: 0 638 1749 Size: 638 1111 BstAPI GCAn_nnn'nTGC Cuts at: 0 858 1749 Size: 858 891 BstEII G'GTnAC_C Cuts at: 0 708 1749 Size: 708 1041 BstYI r'GATC_y Cuts at: 0 422 489 768 1278 1749 Size: 422 67 279 510 471 Fragments arranged by size: 510 471 422 279 67 Bsu36I CC'TnA_GG Cuts at: 0 335 1749 Size: 335 1414 BtgI C'CryG_G Cuts at: 0 824 1241 1638 1749 Size: 824 417 397 111 Fragments arranged by size: 824 417 397 111 BtsI GCAGTG_nn' Cuts at: 0 258 814 873 1591 1749 Size: 258 556 59 718 158 Fragments arranged by size: 718 556 258 158 59 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1346 1381 1749 Size: 1346 35 368 Fragments arranged by size: 1346 368 35 EaeI y'GGCC_r Cuts at: 0 531 1334 1526 1749 Size: 531 803 192 223 Fragments arranged by size: 803 531 223 192 EarI CTCTTCn'nnn_ Cuts at: 0 116 615 1164 1259 1749 Size: 116 499 549 95 490 Fragments arranged by size: 549 499 490 116 95 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 155 449 1012 1092 1135 1283 1441 Size: 97 58 294 563 80 43 148 158 Cuts at: 1441 1457 1503 1687 1749 Size: 16 46 184 62 Fragments arranged by size: 563 294 184 158 148 97 80 62 58 46 43 16 EcoNI CCTnn'n_nnAGG Cuts at: 0 1570 1749 Size: 1570 179 EcoO109I rG'GnC_Cy Cuts at: 0 477 1749 Size: 477 1272 FspI TGC'GCA Cuts at: 0 1350 1749 Size: 1350 399 FspAI rTGC'GCAy Cuts at: 0 1350 1749 Size: 1350 399 HaeII r_GCGC'y Cuts at: 0 1378 1749 Size: 1378 371 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 31 63 331 363 474 506 1077 1109 Size: 31 32 268 32 111 32 571 32 Cuts at: 1109 1749 Size: 640 Fragments arranged by size: 640 571 268 111 32 32 32 32 31 HincII GTy'rAC Cuts at: 0 141 1749 Size: 141 1608 KasI G'GCGC_C Cuts at: 0 1374 1749 Size: 1374 375 MscI TGG'CCA Cuts at: 0 533 1528 1749 Size: 533 995 221 Fragments arranged by size: 995 533 221 MslI CAynn'nnrTG Cuts at: 0 633 760 1630 1749 Size: 633 127 870 119 Fragments arranged by size: 870 633 127 119 MspA1I CmG'CkG Cuts at: 0 836 1503 1749 Size: 836 667 246 Fragments arranged by size: 836 667 246 NaeI GCC'GGC Cuts at: 0 640 1749 Size: 640 1109 NarI GG'CG_CC Cuts at: 0 1375 1749 Size: 1375 374 NcoI C'CATG_G Cuts at: 0 824 1241 1749 Size: 824 417 508 Fragments arranged by size: 824 508 417 NgoMIV G'CCGG_C Cuts at: 0 638 1749 Size: 638 1111 NspI r_CATG'y Cuts at: 0 305 1349 1749 Size: 305 1044 400 Fragments arranged by size: 1044 400 305 PciI A'CATG_T Cuts at: 0 301 1749 Size: 301 1448 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1108 1140 1749 Size: 1108 32 609 Fragments arranged by size: 1108 609 32 PpuMI rG'GwC_Cy Cuts at: 0 477 1749 Size: 477 1272 PstI C_TGCA'G Cuts at: 0 821 1286 1749 Size: 821 465 463 Fragments arranged by size: 821 465 463 PvuII CAG'CTG Cuts at: 0 1503 1749 Size: 1503 246 ScaI AGT'ACT Cuts at: 0 935 1749 Size: 935 814 SfcI C'TryA_G Cuts at: 0 817 1282 1749 Size: 817 465 467 Fragments arranged by size: 817 467 465 SfoI GGC'GCC Cuts at: 0 1376 1749 Size: 1376 373 SpeI A'CTAG_T Cuts at: 0 1476 1749 Size: 1476 273 StyI C'CwwG_G Cuts at: 0 807 824 1241 1749 Size: 807 17 417 508 Fragments arranged by size: 807 508 417 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 906 1749 Size: 906 843 TatI w'GTAC_w Cuts at: 0 933 1749 Size: 933 816 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 182 214 1749 Size: 182 32 1535 Fragments arranged by size: 1535 182 32 XmnI GAAnn'nnTTC Cuts at: 0 278 1025 1105 1749 Size: 278 747 80 644 Fragments arranged by size: 747 644 278 80 Enzymes that do cut: AccI AcuI AflIII AloI AlwNI ApoI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BlpI BmrI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BstAPI BstEII BstYI Bsu36I BtgI BtsI CspCI EaeI EarI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PpiI PpuMI PstI PvuII ScaI SfcI SfoI SpeI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI BaeI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI