################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 124 CGCG CG^CG AspHI 299 GTGCTC G[AT]GC[AT]^C BamHI 236 GGATCC G^GATCC BanI 51 GGCACC G^G[CT][GA]CC BanII 136 GAGCCC G[GA]GC[CT]^C BbvCI 211 GCTGAGG CCTCAGC(-5/-2) BseRI 205 GAGGAG GAGGAG(10/8) BsgI 127 GTGCAG GTGCAG(16/14) BsiHKAI 299 GTGCTC G[AT]GC[AT]^C BsmFI 11 GTCCC GGGAC(10/14) BspHI 113 TCATGA T^CATGA BsrFI 227 GCCGGC [GA]^CCGG[CT] BstF5I 120 CATCC GGATG(2/0) BstUI 124 CGCG CG^CG BstXI 140 CCAAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 236 GGATCC [GA]^GATC[CT] Cfr10I 227 GCCGGC [GA]^CCGG[CT] DpnII 237 GATC ^GATC EcoT14I 47 CCAAGG C^C[AT][AT]GG FauI 317 CCCGC CCCGC(4/6) FokI 120 CATCC GGATG(9/13) HaeII 194 GGCGCT [GA]GCGC^[CT] HapII 228 CCGG C^CGG HpaII 228 CCGG C^CGG MboI 237 GATC ^GATC MboII 31 TCTTC GAAGA(8/7) MflI 236 GGATCC [GA]^GATC[CT] MslI 120 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 190 CAGCGG C[AC]G^C[GT]G MspI 228 CCGG C^CGG MvnI 124 CGCG CG^CG NaeI 227 GCCGGC GCC^GGC NdeII 237 GATC ^GATC NgoMIV 227 GCCGGC G^CCGGC NlaIII 114 CATG CATG^ PstI 245 CTGCAG CTGCA^G RcaI 113 TCATGA T^CATGA Sau3AI 237 GATC ^GATC SfcI 245 CTGCAG C^T[GA][CT]AG StyI 47 CCAAGG C^C[AT][AT]GG XhoII 236 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwI 237 GATCC GGATC(4/5) 236 GGATC AvaII 81 GGACC G^G[AT]CC 327 GGTCC BbvI 231 GCTGC GCAGC(8/12) 244 GCTGC BmyI 136 GAGCCC G[AGT]GC[ACT]^C 299 GTGCTC BsaI 69 GGTCTC GGTCTC(1/5) 150 GGTCTC BsaJI 47 CCAAGG C^C[ACGT][ACGT]GG 330 CCGAGG BsiYI 123 CCGCGTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 318 CCGCTTGGAGG BslI 123 CCGCGTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 318 CCGCTTGGAGG BsmAI 70 GTCTC GTCTC(1/5) 151 GTCTC Bsp1286I 136 GAGCCC G[AGT]GC[ACT]^C 299 GTGCTC BssKI 155 CCTGG ^CC[ACGT]GG 240 CCTGG BstNI 155 CCTGG CC^[AT]GG 240 CCTGG CfoI 180 GCGC GCG^C 195 GCGC DdeI 160 CTGAG C^T[ACGT]AG 212 CTGAG EcoRII 155 CCTGG ^CC[AT]GG 240 CCTGG HaeIII 100 GGCC GG^CC 315 GGCC HhaI 180 GCGC GCG^C 195 GCGC HinP1I 180 GCGC G^CGC 195 GCGC MvaI 155 CCTGG CC^[AT]GG 240 CCTGG ScrFI 155 CCTGG CC^[ACGT]GG 240 CCTGG SfaNI 259 GATGC GCATC(5/9) 119 GCATC TseI 231 GCTGC G^C[AT]GC 244 GCTGC TspRI 39 CACTG CA[GC]TG[ACGT][ACGT]^ 307 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AciI BsrI Cac8I Cfr13I Fnu4HI ItaI MnlI MwoI NlaIV Sau96I