################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI AlwNI AscI AseI AsnI Asp718I AspEI AvrII BbvCI BcgI BfrI BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstBI BstXI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I EcoRV EcoT22I FseI HindIII HpaI KpnI MamI MfeI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 1885 GACGTC GACGT^C AflIII 615 ACGCGT A^C[GA][CT]GT Alw44I 601 GTGCAC G^TGCAC Aor51HI 1157 AGCGCT AGC^GCT ApaI 2057 GGGCCC GGGCC^C ApaLI 601 GTGCAC G^TGCAC AspI 1885 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1277 TGCGCA TGC^GCA BbeI 2083 GGCGCC GGCGC^C BbrPI 1240 CACGTG CAC^GTG BbsI 1110 GAAGAC GAAGAC(2/6) BciVI 840 GTATCC GTATCC(6/5) BclI 1856 TGATCA T^GATCA BfaI 64 CTAG C^TAG BglI 2101 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1110 GAAGAC GAAGAC(2/6) BsaAI 1240 CACGTG [CT]AC^GT[GA] BsaI 1778 GAGACC GGTCTC(1/5) BsgI 1744 CTGCAC GTGCAG(16/14) BsiEI 1976 CGGCCG CG[GA][CT]^CG BsmBI 982 GAGACG CGTCTC(1/5) BsmI 318 GAATGC GAATGC(1/-1) Bsp1407I 1760 TGTACA T^GTACA BspHI 1896 TCATGA T^CATGA BsrDI 131 GCAATG GCAATG(2/0) BsrGI 1760 TGTACA T^GTACA BstAPI 171 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EagI 1976 CGGCCG C^GGCCG EclXI 1976 CGGCCG C^GGCCG Eco47III 1157 AGCGCT AGC^GCT Eco52I 1976 CGGCCG C^GGCCG EcoNI 1647 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 265 GAATTC G^AATTC FbaI 1856 TGATCA T^GATCA FspI 1277 TGCGCA TGC^GCA HgaI 614 GACGC GACGC(5/10) HincII 715 GTCAAC GT[CT]^[GA]AC HindII 715 GTCAAC GT[CT]^[GA]AC KasI 2083 GGCGCC G^GCGCC KspI 1192 CCGCGG CCGC^GG MaeI 64 CTAG C^TAG MluI 615 ACGCGT A^CGCGT NarI 2083 GGCGCC GG^CGCC NcoI 2075 CCATGG C^CATGG PaeR7I 367 CTCGAG C^TCGAG PflFI 1885 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 436 GACTC GAGTC(4/5) PmaCI 1240 CACGTG CAC^GTG PmlI 1240 CACGTG CAC^GTG RcaI 1896 TCATGA T^CATGA SacI 1045 GAGCTC GAGCT^C SacII 1192 CCGCGG CCGC^GG SapI 87 GAAGAGC GCTCTTC(1/4) SfiI 2100 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2083 GGCGCC GGC^GCC SgrAI 1468 CGCCGGTG C[GA]^CCGG[CT]G SspBI 1760 TGTACA T^GTACA Tth111I 1885 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 367 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 265 GAATTC [GA]^AATT[CT] 487 GAATTT AcyI 1885 GACGTC G[GA]^CG[CT]C 2083 GGCGCC AocI 1144 CCTGAGG CC^T[ACGT]AGG 2021 CCTCAGG ApoI 265 GAATTC [GA]^AATT[CT] 487 GAATTT Asp700I 87 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2121 GAACCTCTTC BglII 1997 AGATCT A^GATCT 2471 AGATCT BsaHI 1885 GACGTC G[GA]^CG[CT]C 2083 GGCGCC BsrFI 98 ACCGGC [GA]^CCGG[CT] 1469 GCCGGT BssSI 1714 CACGAG CACGAG(-5/-1) 190 CTCGTG BstEII 1837 GGTTACC G^GT[ACGT]ACC 1869 GGTCACC BstPI 1837 GGTTACC G^GT[ACGT]ACC 1869 GGTCACC Bsu36I 1144 CCTGAGG CC^T[ACGT]AGG 2021 CCTCAGG Cfr10I 98 ACCGGC [GA]^CCGG[CT] 1469 GCCGGT Eco81I 1144 CCTGAGG CC^T[ACGT]AGG 2021 CCTCAGG EcoO65I 1837 GGTTACC G^GT[ACGT]ACC 1869 GGTCACC Hin1I 1885 GACGTC G[GA]^CG[CT]C 2083 GGCGCC MseI 500 TTAA T^TAA 1406 TTAA PflMI 186 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2384 CCAAGGAGTGG ScaI 890 AGTACT AGT^ACT 2192 AGTACT SmaI 1491 CCCGGG CCC^GGG 2522 CCCGGG SmlI 367 CTCGAG C^T[CT][GA]AG 1630 CTCAAG TfiI 476 GAATC G^A[AT]TC 1769 GAATC Tru9I 500 TTAA T^TAA 1406 TTAA Van91I 186 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2384 CCAAGGAGTGG XmaCI 1491 CCCGGG C^CCGGG 2522 CCCGGG XmaI 1491 CCCGGG C^CCGGG 2522 CCCGGG XmnI 87 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2121 GAACCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BalI BamHI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII