(Circular) (Six-base) MAPSORT of: pFN21AE5762.seq Check: 3386 from: 1 to: 7234 pFN21AE5762 7234 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4218 4218 Size: 7234 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3894 5044 278 Size: 53 83 186 936 2358 1150 2468 Fragments arranged by size: 2468 2358 1150 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4577 1937 Size: 2640 4594 Acc65I G'GTAC_C Cuts at: 4555 4555 Size: 7234 AclI AA'CG_TT Cuts at: 1563 5361 5734 1563 Size: 3798 373 3063 Fragments arranged by size: 3798 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2303 2906 3581 4241 5297 6681 6949 1632 Size: 671 603 675 660 1056 1384 268 1917 Fragments arranged by size: 1917 1384 1056 675 671 660 603 268 AfeI AGC'GCT Cuts at: 3164 3164 Size: 7234 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7037 AflIII A'CryG_T Cuts at: 1203 2620 6133 7110 1203 Size: 1417 3513 977 1327 Fragments arranged by size: 3513 1417 1327 977 AgeI A'CCGG_T Cuts at: 6065 6065 Size: 7234 AhdI GACnn_n'nnGTC Cuts at: 5963 5963 Size: 7234 AleI CACnn'nnGTG Cuts at: 1166 2303 2663 2693 3339 1166 Size: 1137 360 30 646 5061 Fragments arranged by size: 5061 1137 646 360 30 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7234 AlwNI CAG_nnn'CTG Cuts at: 4977 6549 4977 Size: 1572 5662 ApaI G_GGCC'C Cuts at: 4066 4066 Size: 7234 ApaLI G'TGCA_C Cuts at: 2606 5291 6447 2606 Size: 2685 1156 3393 Fragments arranged by size: 3393 2685 1156 ApoI r'AATT_y Cuts at: 1493 2270 2492 4543 4769 4921 7030 1493 Size: 777 222 2051 226 152 2109 1697 Fragments arranged by size: 2109 2051 1697 777 226 222 152 AseI AT'TA_AT Cuts at: 160 1032 5788 160 Size: 872 4756 1606 Fragments arranged by size: 4756 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7234 AvaI C'yCGr_G Cuts at: 1870 1943 2372 3496 4214 4429 4527 4559 1870 Size: 73 429 1124 718 215 98 32 4545 Fragments arranged by size: 4545 1124 718 429 215 98 73 32 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3501 3534 3501 Size: 33 7201 BamHI G'GATC_C Cuts at: 2208 3060 3378 3714 4564 4931 2208 Size: 852 318 336 850 367 4511 Fragments arranged by size: 4511 852 850 367 336 318 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3201 3234 4016 4076 4088 Size: 325 217 627 1414 33 782 60 12 Cuts at: 4088 4555 6010 618 Size: 467 1455 1842 Fragments arranged by size: 1842 1455 1414 782 627 467 325 217 60 33 12 BanII G_rGCy'C Cuts at: 1391 3054 4066 4494 4553 1391 Size: 1663 1012 428 59 4072 Fragments arranged by size: 4072 1663 1012 428 59 BbeI G_GCGC'C Cuts at: 4092 4092 Size: 7234 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3122 928 Size: 971 1223 5040 Fragments arranged by size: 5040 1223 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5425 5459 1903 Size: 34 3488 34 3678 Fragments arranged by size: 3678 3488 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2856 5126 6336 2856 Size: 2270 1210 3754 Fragments arranged by size: 3754 2270 1210 BclI T'GATC_A Cuts at: 1548 3861 1548 Size: 2313 4921 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4112 5845 136 Size: 107 122 71 631 605 2440 1733 1525 Fragments arranged by size: 2440 1733 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4002 4476 7229 4002 Size: 474 2753 4007 Fragments arranged by size: 4007 2753 474 BlpI GC'TnA_GC Cuts at: 4651 4651 Size: 7234 Bme1580I G_kGCm'C Cuts at: 1790 2610 2700 3807 4066 5295 6451 1790 Size: 820 90 1107 259 1229 1156 2573 Fragments arranged by size: 2573 1229 1156 1107 820 259 90 BmgBI CAC'GTC Cuts at: 1318 2077 3073 3607 3754 1318 Size: 759 996 534 147 4798 Fragments arranged by size: 4798 996 759 534 147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2687 3097 3902 Size: 416 53 83 399 24 1270 410 805 Cuts at: 3902 4965 5923 442 Size: 1063 958 1753 Fragments arranged by size: 1753 1270 1063 958 805 416 410 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7234 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2848 2848 Size: 7234 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2818 2903 3227 3449 3674 3962 4426 5894 Size: 1579 85 324 222 225 288 464 1468 Cuts at: 5894 1239 Size: 2579 Fragments arranged by size: 2579 1579 1468 464 324 288 225 222 85 Bpu10I CC'TnA_GC Cuts at: 3048 3551 3048 Size: 503 6731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3620 5359 6224 6522 3620 Size: 1739 865 298 4332 Fragments arranged by size: 4332 1739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3777 5897 882 Size: 617 2278 2120 2219 Fragments arranged by size: 2278 2219 2120 617 BsaAI yAC'GTr Cuts at: 493 1204 3247 5025 493 Size: 711 2043 1778 2702 Fragments arranged by size: 2702 2043 1778 711 BsaBI GATnn'nnATC Cuts at: 7228 7228 Size: 7234 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3891 4089 5041 5423 Size: 53 83 186 936 2358 198 952 382 Cuts at: 5423 6058 275 Size: 635 1451 Fragments arranged by size: 2358 1451 952 936 635 382 198 186 83 53 BsaWI w'CCGG_w Cuts at: 2811 4173 4380 4449 5667 6065 6339 6486 6897 Size: 1362 207 69 1218 398 274 147 411 Cuts at: 6897 2811 Size: 3148 Fragments arranged by size: 3148 1362 1218 411 398 274 207 147 69 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3647 3671 3677 3701 1178 Size: 30 165 30 2244 24 6 24 4711 Fragments arranged by size: 4711 2244 165 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 2552 3317 3548 3676 4124 1186 Size: 1204 162 765 231 128 448 4296 Fragments arranged by size: 4296 1204 765 448 231 162 128 BseYI C'CCAG_C Cuts at: 6437 6933 6437 Size: 496 6738 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3734 1163 Size: 573 1998 4663 Fragments arranged by size: 4663 1998 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3984 4684 5445 6473 1540 Size: 275 186 1983 700 761 1028 2301 Fragments arranged by size: 2301 1983 1028 761 700 275 186 BsiHKAI G_wGCw'C Cuts at: 2610 2631 3054 5295 5380 6451 2610 Size: 21 423 2241 85 1071 3393 Fragments arranged by size: 3393 2241 1071 423 85 21 BsmI GAATG_Cn' Cuts at: 2329 4745 4838 2329 Size: 2416 93 4725 Fragments arranged by size: 4725 2416 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2981 2981 Size: 7234 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2610 2631 2700 3054 3807 4066 4494 Size: 399 820 21 69 354 753 259 428 Cuts at: 4494 4553 5295 5380 6451 1391 Size: 59 742 85 1071 2174 Fragments arranged by size: 2174 1071 820 753 742 428 399 354 259 85 69 59 21 BspEI T'CCGG_A Cuts at: 6897 6897 Size: 7234 BspHI T'CATG_A Cuts at: 3901 5123 6853 3901 Size: 1222 1730 4282 Fragments arranged by size: 4282 1730 1222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2521 4218 4509 4589 844 Size: 1677 1697 291 80 3489 Fragments arranged by size: 3489 1697 1677 291 80 BsrBI CCG'CTC Cuts at: 1394 1823 5121 1394 Size: 429 3298 3507 Fragments arranged by size: 3507 3298 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2143 5729 5903 65 Size: 1183 895 3586 174 1396 Fragments arranged by size: 3586 1396 1183 895 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2103 3474 5878 6065 1700 Size: 110 293 1371 2404 187 2869 Fragments arranged by size: 2869 2404 1371 293 187 110 BsrGI T'GTAC_A Cuts at: 96 3765 96 Size: 3669 3565 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7234 BssSI C'ACGA_G Cuts at: 2195 3719 5294 6306 2195 Size: 1524 1575 1012 3123 Fragments arranged by size: 3123 1575 1524 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2182 794 Size: 1388 5846 BstBI TT'CG_AA Cuts at: 6038 6038 Size: 7234 BstEII G'GTnAC_C Cuts at: 1184 3842 3874 1184 Size: 2658 32 4544 Fragments arranged by size: 4544 2658 32 BstYI r'GATC_y Cuts at: 1972 2208 3060 3378 3714 4002 4476 4506 4564 Size: 236 852 318 336 288 474 30 58 Cuts at: 4564 4931 5316 5333 6785 6871 6883 7229 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 852 474 385 367 346 336 318 288 236 86 58 30 17 12 Bsu36I CC'TnA_GG Cuts at: 3150 4027 3150 Size: 877 6357 BtgI C'CryG_G Cuts at: 513 1065 2004 3197 4080 4230 513 Size: 552 939 1193 883 150 3517 Fragments arranged by size: 3517 1193 939 883 552 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2346 3282 4405 487 Size: 683 56 1120 936 1123 3316 Fragments arranged by size: 3316 1123 1120 936 683 56 BtsI GCAGTG_nn' Cuts at: 4008 4252 4358 4750 5544 5564 4008 Size: 244 106 392 794 20 5678 Fragments arranged by size: 5678 794 392 244 106 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7234 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7199 DraI TTT'AAA Cuts at: 4538 4538 Size: 7234 DraIII CAC_nnn'GTG Cuts at: 2182 2197 3142 2182 Size: 15 945 6274 Fragments arranged by size: 6274 945 15 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6241 809 Size: 507 4925 1802 Fragments arranged by size: 4925 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2142 2673 Size: 54 1425 50 275 18 249 63 531 Cuts at: 2673 2907 3006 3249 3504 3609 3789 3852 3981 Size: 234 99 243 255 105 180 63 129 Cuts at: 3981 4104 4681 5570 7199 8 Size: 123 577 889 1629 43 Fragments arranged by size: 1629 1425 889 577 531 275 255 249 243 234 180 129 123 105 99 63 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3981 4681 1537 Size: 275 2169 700 4090 Fragments arranged by size: 4090 2169 700 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 3704 4136 5164 1353 Size: 734 1617 432 1028 3423 Fragments arranged by size: 3423 1617 1028 734 432 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2731 2921 5821 6195 6341 387 Size: 1064 138 1142 190 2900 374 146 1280 Fragments arranged by size: 2900 1280 1142 1064 374 190 146 138 EcoICRI GAG'CTC Cuts at: 3052 3052 Size: 7234 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 2818 2903 2906 3227 3449 3581 Size: 393 671 515 85 3 321 222 132 Cuts at: 3581 3674 3962 4241 4426 5297 5894 6681 6949 Size: 93 288 279 185 871 597 787 268 Cuts at: 6949 1239 Size: 1524 Fragments arranged by size: 1524 871 787 671 597 515 393 321 288 279 268 222 185 132 93 85 3 EcoNI CCTnn'n_nnAGG Cuts at: 3656 3656 Size: 7234 EcoO109I rG'GnC_Cy Cuts at: 2839 2965 3351 4062 4063 2839 Size: 126 386 711 1 6010 Fragments arranged by size: 6010 711 386 126 1 EcoRI G'AATT_C Cuts at: 2270 4543 4921 2270 Size: 2273 378 4583 Fragments arranged by size: 4583 2273 378 FspI TGC'GCA Cuts at: 3284 5740 3284 Size: 2456 4778 HaeII r_GCGC'y Cuts at: 1678 3166 3839 4092 6381 1678 Size: 1488 673 253 2289 2531 Fragments arranged by size: 2531 2289 1488 673 253 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2778 2810 5875 5907 5949 Size: 32 135 32 1206 32 3065 32 42 Cuts at: 5949 5981 1373 Size: 32 2626 Fragments arranged by size: 3065 2626 1206 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2722 4578 4824 669 Size: 988 281 784 1856 246 3079 Fragments arranged by size: 3079 1856 988 784 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7234 HpaI GTT'AAC Cuts at: 4824 4824 Size: 7234 KasI G'GCGC_C Cuts at: 4088 4088 Size: 7234 KpnI G_GTAC'C Cuts at: 4559 4559 Size: 7234 MfeI C'AATT_G Cuts at: 4833 4833 Size: 7234 MluI A'CGCG_T Cuts at: 2620 7110 2620 Size: 4490 2744 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4521 6348 6532 1305 Size: 3216 1827 184 2007 Fragments arranged by size: 3216 2007 1827 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 2675 3008 3611 3854 Size: 54 1425 343 312 531 333 603 243 Cuts at: 3854 10 Size: 3390 Fragments arranged by size: 3390 1425 603 531 343 333 312 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 2303 2663 2693 3339 4301 5192 5551 Size: 648 1137 360 30 646 962 891 359 Cuts at: 5551 5710 518 Size: 159 2042 Fragments arranged by size: 2042 1137 962 891 648 646 360 359 159 30 MspA1I CmG'CkG Cuts at: 1606 2017 2205 3016 3199 3232 3559 3577 3736 Size: 411 188 811 183 33 327 18 159 Cuts at: 3736 3986 4375 4650 5327 6475 6720 1606 Size: 250 389 275 677 1148 245 2120 Fragments arranged by size: 2120 1148 811 677 411 389 327 275 250 245 188 183 159 33 18 NarI GG'CG_CC Cuts at: 4089 4089 Size: 7234 NcoI C'CATG_G Cuts at: 513 1065 2004 4080 513 Size: 552 939 2076 3667 Fragments arranged by size: 3667 2076 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7234 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7234 NotI GC'GGCC_GC Cuts at: 4681 4681 Size: 7234 NspI r_CATG'y Cuts at: 1132 1229 3707 4300 4351 4592 6137 1132 Size: 97 2478 593 51 241 1545 2229 Fragments arranged by size: 2478 2229 1545 593 241 97 51 PasI CC'CwG_GG Cuts at: 1348 2571 1348 Size: 1223 6011 PciI A'CATG_T Cuts at: 6133 6133 Size: 7234 PflMI CCAn_nnn'nTGG Cuts at: 1108 2197 4395 1108 Size: 1089 2198 3947 Fragments arranged by size: 3947 2198 1089 PfoI T'CCnGG_A Cuts at: 2805 2919 3651 3939 2805 Size: 114 732 288 6100 Fragments arranged by size: 6100 732 288 114 PmeI GTTT'AAAC Cuts at: 4538 4538 Size: 7234 PmlI CAC'GTG Cuts at: 3247 3247 Size: 7234 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5303 5335 6816 6848 5303 Size: 32 1481 32 5689 Fragments arranged by size: 5689 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2839 2965 3351 2839 Size: 126 386 6722 Fragments arranged by size: 6722 386 126 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7234 PsiI TTA'TAA Cuts at: 4804 4804 Size: 7234 PspOMI G'GGCC_C Cuts at: 4062 4062 Size: 7234 PspXI vC'TCGA_Gb Cuts at: 2372 2372 Size: 7234 PstI C_TGCA'G Cuts at: 830 2295 2772 4015 4506 4586 830 Size: 1465 477 1243 491 80 3478 Fragments arranged by size: 3478 1465 1243 491 477 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7234 PvuII CAG'CTG Cuts at: 3016 3559 3736 4375 3016 Size: 543 177 639 5875 Fragments arranged by size: 5875 639 543 177 SacI G_AGCT'C Cuts at: 3054 3054 Size: 7234 SacII CC_GC'GG Cuts at: 3200 3200 Size: 7234 SalI G'TCGA_C Cuts at: 1936 4576 1936 Size: 2640 4594 SanDI GG'GwC_CC Cuts at: 2965 3351 2965 Size: 386 6848 SapI GCTCTTCn'nnn_ Cuts at: 2087 2087 Size: 7234 SbfI CC_TGCA'GG Cuts at: 4586 4586 Size: 7234 ScaI AGT'ACT Cuts at: 2897 4199 5482 7194 2897 Size: 1302 1283 1712 2937 Fragments arranged by size: 2937 1712 1302 1283 SfcI C'TryA_G Cuts at: 826 1045 2291 2768 4011 4502 4582 5717 6398 Size: 219 1246 477 1243 491 80 1135 681 Cuts at: 6398 6589 826 Size: 191 1471 Fragments arranged by size: 1471 1246 1243 1135 681 491 477 219 191 80 SfiI GGCCn_nnn'nGGCC Cuts at: 4112 4112 Size: 7234 SfoI GGC'GCC Cuts at: 4090 4090 Size: 7234 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7234 SgrAI Cr'CCGG_yG Cuts at: 1700 3474 1700 Size: 1774 5460 SmaI CCC'GGG Cuts at: 1872 3498 4529 4561 1872 Size: 1626 1031 32 4545 Fragments arranged by size: 4545 1626 1031 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 2372 3635 5338 6239 6501 820 Size: 197 926 429 1263 1703 901 262 1553 Fragments arranged by size: 1703 1553 1263 926 901 429 262 197 SnaBI TAC'GTA Cuts at: 493 5025 493 Size: 4532 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7234 SphI G_CATG'C Cuts at: 1132 4592 1132 Size: 3460 3774 SspI AAT'ATT Cuts at: 5 52 5158 5 Size: 47 5106 2081 Fragments arranged by size: 5106 2081 47 StuI AGG'CCT Cuts at: 1517 2412 3098 3155 4025 4166 1517 Size: 895 686 57 870 141 4585 Fragments arranged by size: 4585 895 870 686 141 57 StyI C'CwwG_G Cuts at: 513 1065 2004 2398 2454 2745 3156 3612 3792 Size: 552 939 394 56 291 411 456 180 Cuts at: 3792 3960 4080 4167 4263 4389 4673 513 Size: 168 120 87 96 126 284 3074 Fragments arranged by size: 3074 939 552 456 411 394 291 284 180 168 126 120 96 87 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3041 3394 3905 5261 5278 5431 5616 Size: 1255 1461 353 511 1356 17 153 185 Cuts at: 5616 6061 325 Size: 445 1498 Fragments arranged by size: 1498 1461 1356 1255 511 445 353 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2895 3663 3723 Size: 274 80 33 51 1443 918 768 60 Cuts at: 3723 3765 4197 5480 7192 96 Size: 42 432 1283 1712 138 Fragments arranged by size: 1712 1443 1283 918 768 432 274 138 80 60 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7194 7226 7194 Size: 32 7202 Tth111I GACn'n_nGTC Cuts at: 1316 3893 1316 Size: 2577 4657 XbaI T'CTAG_A Cuts at: 4570 4570 Size: 7234 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2347 3362 4078 4099 4244 2347 Size: 1015 716 21 145 5337 Fragments arranged by size: 5337 1015 716 145 21 XhoI C'TCGA_G Cuts at: 1943 2372 1943 Size: 429 6805 XmaI C'CCGG_G Cuts at: 1870 3496 4527 4559 1870 Size: 1626 1031 32 4545 Fragments arranged by size: 4545 1626 1031 32 XmnI GAAnn'nnTTC Cuts at: 2096 4130 5363 2096 Size: 2034 1233 3967 Fragments arranged by size: 3967 2034 1233 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3892 5042 276 Size: 53 83 186 936 2358 1150 2468 Fragments arranged by size: 2468 2358 1150 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbvCI BfrBI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PsrI RsrII SexAI SrfI SwaI