(Linear) (Six-base) MAPSORT of: KIEE5762.seq Check: 9686 from: 1 to: 2529 KIEE5762.orf With 182 enzymes: SgfI * December 5, 2014 13:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2213 2529 Size: 2213 316 AatII G_ACGT'C Cuts at: 0 1889 2529 Size: 1889 640 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 901 1576 2236 2529 Size: 298 603 675 660 293 Fragments arranged by size: 675 660 603 298 293 AfeI AGC'GCT Cuts at: 0 1159 2529 Size: 1159 1370 AflIII A'CryG_T Cuts at: 0 615 2529 Size: 615 1914 AleI CACnn'nnGTG Cuts at: 0 298 658 688 1334 2529 Size: 298 360 30 646 1195 Fragments arranged by size: 1195 646 360 298 30 ApaI G_GGCC'C Cuts at: 0 2061 2529 Size: 2061 468 ApaLI G'TGCA_C Cuts at: 0 601 2529 Size: 601 1928 ApoI r'AATT_y Cuts at: 0 265 487 2529 Size: 265 222 2042 Fragments arranged by size: 2042 265 222 AvaI C'yCGr_G Cuts at: 0 367 1491 2209 2424 2522 2529 Size: 367 1124 718 215 98 7 Fragments arranged by size: 1124 718 367 215 98 7 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1496 1529 2529 Size: 1496 33 1000 Fragments arranged by size: 1496 1000 33 BamHI G'GATC_C Cuts at: 0 203 1055 1373 1709 2529 Size: 203 852 318 336 820 Fragments arranged by size: 852 820 336 318 203 BanI G'GyrC_C Cuts at: 0 1196 1229 2011 2071 2083 2529 Size: 1196 33 782 60 12 446 Fragments arranged by size: 1196 782 446 60 33 12 BanII G_rGCy'C Cuts at: 0 1049 2061 2489 2529 Size: 1049 1012 428 40 Fragments arranged by size: 1049 1012 428 40 BbeI G_GCGC'C Cuts at: 0 2087 2529 Size: 2087 442 BbsI GAAGACnn'nnnn_ Cuts at: 0 1117 2529 Size: 1117 1412 BciVI GTATCCnnnnn_n' Cuts at: 0 851 2529 Size: 851 1678 BclI T'GATC_A Cuts at: 0 1856 2529 Size: 1856 673 BglI GCCn_nnn'nGGC Cuts at: 0 2107 2529 Size: 2107 422 BglII A'GATC_T Cuts at: 0 1997 2471 2529 Size: 1997 474 58 Fragments arranged by size: 1997 474 58 Bme1580I G_kGCm'C Cuts at: 0 605 695 1802 2061 2529 Size: 605 90 1107 259 468 Fragments arranged by size: 1107 605 468 259 90 BmgBI CAC'GTC Cuts at: 0 72 1068 1602 1749 2529 Size: 72 996 534 147 780 Fragments arranged by size: 996 780 534 147 72 BmrI ACTGGGnnnn_n' Cuts at: 0 682 1092 1897 2529 Size: 682 410 805 632 Fragments arranged by size: 805 682 632 410 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 843 2529 Size: 843 1686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 813 898 1222 1444 1669 1957 2421 2529 Size: 813 85 324 222 225 288 464 108 Fragments arranged by size: 813 464 324 288 225 222 108 85 Bpu10I CC'TnA_GC Cuts at: 0 1043 1546 2529 Size: 1043 503 983 Fragments arranged by size: 1043 983 503 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1615 2529 Size: 1615 914 BsaI GGTCTCn'nnnn_ Cuts at: 0 1772 2529 Size: 1772 757 BsaAI yAC'GTr Cuts at: 0 1242 2529 Size: 1242 1287 BsaHI Gr'CG_yC Cuts at: 0 1886 2084 2529 Size: 1886 198 445 Fragments arranged by size: 1886 445 198 BsaWI w'CCGG_w Cuts at: 0 806 2168 2375 2444 2529 Size: 806 1362 207 69 85 Fragments arranged by size: 1362 806 207 85 69 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1642 1666 1672 1696 2529 Size: 1642 24 6 24 833 Fragments arranged by size: 1642 833 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 385 547 1312 1543 1671 2119 2529 Size: 385 162 765 231 128 448 410 Fragments arranged by size: 765 448 410 385 231 162 128 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1729 2529 Size: 1729 800 BsiEI CG_ry'CG Cuts at: 0 1979 2529 Size: 1979 550 BsiHKAI G_wGCw'C Cuts at: 0 605 626 1049 2529 Size: 605 21 423 1480 Fragments arranged by size: 1480 605 423 21 BsmI GAATG_Cn' Cuts at: 0 324 2529 Size: 324 2205 BsmBI CGTCTCn'nnnn_ Cuts at: 0 976 2529 Size: 976 1553 Bsp1286I G_dGCh'C Cuts at: 0 605 626 695 1049 1802 2061 2489 2529 Size: 605 21 69 354 753 259 428 40 Fragments arranged by size: 753 605 428 354 259 69 40 21 BspHI T'CATG_A Cuts at: 0 1896 2529 Size: 1896 633 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 516 2213 2504 2529 Size: 516 1697 291 25 Fragments arranged by size: 1697 516 291 25 BsrDI GCAATG_nn' Cuts at: 0 138 2529 Size: 138 2391 BsrFI r'CCGG_y Cuts at: 0 98 1469 2529 Size: 98 1371 1060 Fragments arranged by size: 1371 1060 98 BsrGI T'GTAC_A Cuts at: 0 1760 2529 Size: 1760 769 BssSI C'ACGA_G Cuts at: 0 190 1714 2529 Size: 190 1524 815 Fragments arranged by size: 1524 815 190 BstAPI GCAn_nnn'nTGC Cuts at: 0 177 2529 Size: 177 2352 BstEII G'GTnAC_C Cuts at: 0 1837 1869 2529 Size: 1837 32 660 Fragments arranged by size: 1837 660 32 BstYI r'GATC_y Cuts at: 0 203 1055 1373 1709 1997 2471 2501 2529 Size: 203 852 318 336 288 474 30 28 Fragments arranged by size: 852 474 336 318 288 203 30 28 Bsu36I CC'TnA_GG Cuts at: 0 1145 2022 2529 Size: 1145 877 507 Fragments arranged by size: 1145 877 507 BtgI C'CryG_G Cuts at: 0 1192 2075 2225 2529 Size: 1192 883 150 304 Fragments arranged by size: 1192 883 304 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 341 1277 2400 2529 Size: 341 936 1123 129 Fragments arranged by size: 1123 936 341 129 BtsI GCAGTG_nn' Cuts at: 0 2003 2247 2353 2529 Size: 2003 244 106 176 Fragments arranged by size: 2003 244 176 106 DraIII CAC_nnn'GTG Cuts at: 0 177 192 1137 2529 Size: 177 15 945 1392 Fragments arranged by size: 1392 945 177 15 EaeI y'GGCC_r Cuts at: 0 74 137 668 902 1001 1244 1499 1604 Size: 74 63 531 234 99 243 255 105 Cuts at: 1604 1784 1847 1976 2099 2529 Size: 180 63 129 123 430 Fragments arranged by size: 531 430 255 243 234 180 129 123 105 99 74 63 63 EagI C'GGCC_G Cuts at: 0 1976 2529 Size: 1976 553 EarI CTCTTCn'nnn_ Cuts at: 0 82 1699 2131 2529 Size: 82 1617 432 398 Fragments arranged by size: 1617 432 398 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 726 916 2529 Size: 726 190 1613 Fragments arranged by size: 1613 726 190 EcoICRI GAG'CTC Cuts at: 0 1047 2529 Size: 1047 1482 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 813 898 901 1222 1444 1576 1669 Size: 298 515 85 3 321 222 132 93 Cuts at: 1669 1957 2236 2421 2529 Size: 288 279 185 108 Fragments arranged by size: 515 321 298 288 279 222 185 132 108 93 85 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 1651 2529 Size: 1651 878 EcoO109I rG'GnC_Cy Cuts at: 0 834 960 1346 2057 2058 2529 Size: 834 126 386 711 1 471 Fragments arranged by size: 834 711 471 386 126 1 EcoRI G'AATT_C Cuts at: 0 265 2529 Size: 265 2264 FspI TGC'GCA Cuts at: 0 1279 2529 Size: 1279 1250 HaeII r_GCGC'y Cuts at: 0 1161 1834 2087 2529 Size: 1161 673 253 442 Fragments arranged by size: 1161 673 442 253 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 773 805 2529 Size: 773 32 1724 Fragments arranged by size: 1724 773 32 HincII GTy'rAC Cuts at: 0 717 2529 Size: 717 1812 KasI G'GCGC_C Cuts at: 0 2083 2529 Size: 2083 446 MluI A'CGCG_T Cuts at: 0 615 2529 Size: 615 1914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2516 2529 Size: 2516 13 MscI TGG'CCA Cuts at: 0 139 670 1003 1606 1849 2529 Size: 139 531 333 603 243 680 Fragments arranged by size: 680 603 531 333 243 139 MslI CAynn'nnrTG Cuts at: 0 298 658 688 1334 2296 2529 Size: 298 360 30 646 962 233 Fragments arranged by size: 962 646 360 298 233 30 MspA1I CmG'CkG Cuts at: 0 12 200 1011 1194 1227 1554 1572 1731 Size: 12 188 811 183 33 327 18 159 Cuts at: 1731 1981 2370 2529 Size: 250 389 159 Fragments arranged by size: 811 389 327 250 188 183 159 159 33 18 12 NarI GG'CG_CC Cuts at: 0 2084 2529 Size: 2084 445 NcoI C'CATG_G Cuts at: 0 2075 2529 Size: 2075 454 NspI r_CATG'y Cuts at: 0 1702 2295 2346 2529 Size: 1702 593 51 183 Fragments arranged by size: 1702 593 183 51 PasI CC'CwG_GG Cuts at: 0 566 2529 Size: 566 1963 PflMI CCAn_nnn'nTGG Cuts at: 0 192 2390 2529 Size: 192 2198 139 Fragments arranged by size: 2198 192 139 PfoI T'CCnGG_A Cuts at: 0 800 914 1646 1934 2529 Size: 800 114 732 288 595 Fragments arranged by size: 800 732 595 288 114 PmlI CAC'GTG Cuts at: 0 1242 2529 Size: 1242 1287 PpuMI rG'GwC_Cy Cuts at: 0 834 960 1346 2529 Size: 834 126 386 1183 Fragments arranged by size: 1183 834 386 126 PspOMI G'GGCC_C Cuts at: 0 2057 2529 Size: 2057 472 PspXI vC'TCGA_Gb Cuts at: 0 367 2529 Size: 367 2162 PstI C_TGCA'G Cuts at: 0 290 767 2010 2501 2529 Size: 290 477 1243 491 28 Fragments arranged by size: 1243 491 477 290 28 PvuII CAG'CTG Cuts at: 0 1011 1554 1731 2370 2529 Size: 1011 543 177 639 159 Fragments arranged by size: 1011 639 543 177 159 SacI G_AGCT'C Cuts at: 0 1049 2529 Size: 1049 1480 SacII CC_GC'GG Cuts at: 0 1195 2529 Size: 1195 1334 SanDI GG'GwC_CC Cuts at: 0 960 1346 2529 Size: 960 386 1183 Fragments arranged by size: 1183 960 386 SapI GCTCTTCn'nnn_ Cuts at: 0 82 2529 Size: 82 2447 ScaI AGT'ACT Cuts at: 0 892 2194 2529 Size: 892 1302 335 Fragments arranged by size: 1302 892 335 SfcI C'TryA_G Cuts at: 0 286 763 2006 2497 2529 Size: 286 477 1243 491 32 Fragments arranged by size: 1243 491 477 286 32 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2107 2529 Size: 2107 422 SfoI GGC'GCC Cuts at: 0 2085 2529 Size: 2085 444 SgrAI Cr'CCGG_yG Cuts at: 0 1469 2529 Size: 1469 1060 SmaI CCC'GGG Cuts at: 0 1493 2524 2529 Size: 1493 1031 5 Fragments arranged by size: 1493 1031 5 SmlI C'TyrA_G Cuts at: 0 367 1630 2529 Size: 367 1263 899 Fragments arranged by size: 1263 899 367 StuI AGG'CCT Cuts at: 0 407 1093 1150 2020 2161 2529 Size: 407 686 57 870 141 368 Fragments arranged by size: 870 686 407 368 141 57 StyI C'CwwG_G Cuts at: 0 393 449 740 1151 1607 1787 1955 2075 Size: 393 56 291 411 456 180 168 120 Cuts at: 2075 2162 2258 2384 2529 Size: 87 96 126 145 Fragments arranged by size: 456 411 393 291 180 168 145 126 120 96 87 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1036 1389 1900 2529 Size: 1036 353 511 629 Fragments arranged by size: 1036 629 511 353 TatI w'GTAC_w Cuts at: 0 890 1658 1718 1760 2192 2529 Size: 890 768 60 42 432 337 Fragments arranged by size: 890 768 432 337 60 42 Tth111I GACn'n_nGTC Cuts at: 0 1888 2529 Size: 1888 641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 342 1357 2073 2094 2239 2529 Size: 342 1015 716 21 145 290 Fragments arranged by size: 1015 716 342 290 145 21 XhoI C'TCGA_G Cuts at: 0 367 2529 Size: 367 2162 XmaI C'CCGG_G Cuts at: 0 1491 2522 2529 Size: 1491 1031 7 Fragments arranged by size: 1491 1031 7 XmnI GAAnn'nnTTC Cuts at: 0 91 2125 2529 Size: 91 2034 404 Fragments arranged by size: 2034 404 91 ZraI GAC'GTC Cuts at: 0 1887 2529 Size: 1887 642 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AleI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII KasI MluI MmeI MscI MslI MspA1I NarI NcoI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI SapI ScaI SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AgeI AhdI AlfI AloI AlwNI AscI AseI AsiSI AvrII BbvCI BcgI BfrBI BlpI BmtI BsaBI BseYI BsiWI BspEI BsrBI BssHII BstBI BstXI BstZ17I ClaI CspCI DraI DrdI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SalI SbfI SexAI SgfI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI