(Linear) (Six-base) MAPSORT of: KIEE6786.seq Check: 774 from: 1 to: 1887 KIEE6786.orf With 182 enzymes: SgfI * March 16, 2015 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1365 1887 Size: 1365 522 AccI GT'mk_AC Cuts at: 0 460 1887 Size: 460 1427 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 913 1887 Size: 913 974 AflIII A'CryG_T Cuts at: 0 1227 1887 Size: 1227 660 AhdI GACnn_n'nnGTC Cuts at: 0 456 1887 Size: 456 1431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 83 385 767 1702 1887 Size: 83 302 382 935 185 Fragments arranged by size: 935 382 302 185 83 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1537 1569 1887 Size: 1537 32 318 Fragments arranged by size: 1537 318 32 AlwNI CAG_nnn'CTG Cuts at: 0 372 499 951 1102 1653 1887 Size: 372 127 452 151 551 234 Fragments arranged by size: 551 452 372 234 151 127 ApaI G_GGCC'C Cuts at: 0 1060 1887 Size: 1060 827 ApaLI G'TGCA_C Cuts at: 0 1245 1735 1887 Size: 1245 490 152 Fragments arranged by size: 1245 490 152 ApoI r'AATT_y Cuts at: 0 1777 1887 Size: 1777 110 AvaI C'yCGr_G Cuts at: 0 575 993 1006 1060 1213 1669 1887 Size: 575 418 13 54 153 456 218 Fragments arranged by size: 575 456 418 218 153 54 13 BanI G'GyrC_C Cuts at: 0 1105 1576 1887 Size: 1105 471 311 Fragments arranged by size: 1105 471 311 BanII G_rGCy'C Cuts at: 0 1028 1060 1075 1145 1887 Size: 1028 32 15 70 742 Fragments arranged by size: 1028 742 70 32 15 BbeI G_GCGC'C Cuts at: 0 1109 1887 Size: 1109 778 BbsI GAAGACnn'nnnn_ Cuts at: 0 416 1650 1887 Size: 416 1234 237 Fragments arranged by size: 1234 416 237 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 556 590 1429 1463 1887 Size: 556 34 839 34 424 Fragments arranged by size: 839 556 424 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1574 1887 Size: 1574 313 BlpI GC'TnA_GC Cuts at: 0 1159 1887 Size: 1159 728 Bme1580I G_kGCm'C Cuts at: 0 650 1060 1249 1579 1739 1887 Size: 650 410 189 330 160 148 Fragments arranged by size: 650 410 330 189 160 148 BmgBI CAC'GTC Cuts at: 0 165 458 1887 Size: 165 293 1429 Fragments arranged by size: 1429 293 165 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 151 832 928 1129 1248 1840 1887 Size: 103 48 681 96 201 119 592 47 Fragments arranged by size: 681 592 201 119 103 96 48 47 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 1887 Size: 70 1817 BsaI GGTCTCn'nnnn_ Cuts at: 0 1266 1887 Size: 1266 621 BsaBI GATnn'nnATC Cuts at: 0 592 1887 Size: 592 1295 BsaHI Gr'CG_yC Cuts at: 0 1106 1887 Size: 1106 781 BsaWI w'CCGG_w Cuts at: 0 1871 1887 Size: 1871 16 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 872 902 1813 1843 1850 1861 1880 1887 Size: 872 30 911 30 7 11 19 7 Fragments arranged by size: 911 872 30 30 19 11 7 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 370 648 651 796 1887 Size: 370 278 3 145 1091 Fragments arranged by size: 1091 370 278 145 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 727 937 1399 1887 Size: 145 582 210 462 488 Fragments arranged by size: 582 488 462 210 145 BsiEI CG_ry'CG Cuts at: 0 1127 1887 Size: 1127 760 BsiHKAI G_wGCw'C Cuts at: 0 137 1000 1249 1739 1887 Size: 137 863 249 490 148 Fragments arranged by size: 863 490 249 148 137 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1853 1887 Size: 1853 34 Bsp1286I G_dGCh'C Cuts at: 0 137 650 1000 1028 1060 1075 1145 1249 Size: 137 513 350 28 32 15 70 104 Cuts at: 1249 1579 1739 1887 Size: 330 160 148 Fragments arranged by size: 513 350 330 160 148 137 104 70 32 28 15 BspHI T'CATG_A Cuts at: 0 1323 1631 1887 Size: 1323 308 256 Fragments arranged by size: 1323 308 256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1365 1887 Size: 1365 522 BsrBI CCG'CTC Cuts at: 0 491 942 1724 1887 Size: 491 451 782 163 Fragments arranged by size: 782 491 451 163 BstXI CCAn_nnnn'nTGG Cuts at: 0 321 1887 Size: 321 1566 BstYI r'GATC_y Cuts at: 0 1532 1887 Size: 1532 355 BtgI C'CryG_G Cuts at: 0 1109 1887 Size: 1109 778 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1184 1887 Size: 1184 703 DraIII CAC_nnn'GTG Cuts at: 0 803 1887 Size: 803 1084 EaeI y'GGCC_r Cuts at: 0 143 474 639 1112 1124 1181 1887 Size: 143 331 165 473 12 57 706 Fragments arranged by size: 706 473 331 165 143 57 12 EagI C'GGCC_G Cuts at: 0 1124 1887 Size: 1124 763 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 727 1545 1887 Size: 727 818 342 Fragments arranged by size: 818 727 342 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 151 832 913 928 1129 1248 1840 Size: 103 48 681 81 15 201 119 592 Cuts at: 1840 1887 Size: 47 Fragments arranged by size: 681 592 201 119 103 81 48 47 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 1198 1887 Size: 1198 689 EcoO109I rG'GnC_Cy Cuts at: 0 1032 1057 1206 1718 1887 Size: 1032 25 149 512 169 Fragments arranged by size: 1032 512 169 149 25 EcoRI G'AATT_C Cuts at: 0 1777 1887 Size: 1777 110 EcoRV GAT'ATC Cuts at: 0 1432 1887 Size: 1432 455 FspI TGC'GCA Cuts at: 0 934 1887 Size: 934 953 HaeII r_GCGC'y Cuts at: 0 1109 1887 Size: 1109 778 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 442 474 872 904 954 986 1524 1556 Size: 442 32 398 32 50 32 538 32 Cuts at: 1556 1861 1887 Size: 305 26 Fragments arranged by size: 538 442 398 305 50 32 32 32 32 26 HincII GTy'rAC Cuts at: 0 248 461 1869 1887 Size: 248 213 1408 18 Fragments arranged by size: 1408 248 213 18 HpaI GTT'AAC Cuts at: 0 248 1887 Size: 248 1639 KasI G'GCGC_C Cuts at: 0 1105 1887 Size: 1105 782 MluI A'CGCG_T Cuts at: 0 1227 1887 Size: 1227 660 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 629 1490 1622 1887 Size: 629 861 132 265 Fragments arranged by size: 861 629 265 132 MscI TGG'CCA Cuts at: 0 476 641 1887 Size: 476 165 1246 Fragments arranged by size: 1246 476 165 MslI CAynn'nnrTG Cuts at: 0 749 1630 1759 1887 Size: 749 881 129 128 Fragments arranged by size: 881 749 129 128 MspA1I CmG'CkG Cuts at: 0 369 810 1136 1158 1166 1887 Size: 369 441 326 22 8 721 Fragments arranged by size: 721 441 369 326 22 8 NarI GG'CG_CC Cuts at: 0 1106 1887 Size: 1106 781 NruI TCG'CGA Cuts at: 0 8 1887 Size: 8 1879 NspI r_CATG'y Cuts at: 0 1689 1758 1887 Size: 1689 69 129 Fragments arranged by size: 1689 129 69 PasI CC'CwG_GG Cuts at: 0 1854 1887 Size: 1854 33 PfoI T'CCnGG_A Cuts at: 0 944 1887 Size: 944 943 PpuMI rG'GwC_Cy Cuts at: 0 1206 1718 1887 Size: 1206 512 169 Fragments arranged by size: 1206 512 169 PshAI GACnn'nnGTC Cuts at: 0 1313 1887 Size: 1313 574 PspOMI G'GGCC_C Cuts at: 0 1056 1887 Size: 1056 831 PvuII CAG'CTG Cuts at: 0 369 810 1158 1166 1887 Size: 369 441 348 8 721 Fragments arranged by size: 721 441 369 348 8 SalI G'TCGA_C Cuts at: 0 459 1887 Size: 459 1428 SexAI A'CCwGG_T Cuts at: 0 11 1887 Size: 11 1876 SfcI C'TryA_G Cuts at: 0 984 1887 Size: 984 903 SfoI GGC'GCC Cuts at: 0 1107 1887 Size: 1107 780 SmaI CCC'GGG Cuts at: 0 1215 1671 1887 Size: 1215 456 216 Fragments arranged by size: 1215 456 216 SmlI C'TyrA_G Cuts at: 0 85 1887 Size: 85 1802 SphI G_CATG'C Cuts at: 0 1689 1887 Size: 1689 198 StuI AGG'CCT Cuts at: 0 982 1887 Size: 982 905 StyI C'CwwG_G Cuts at: 0 221 1887 Size: 221 1666 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 551 1382 1714 1810 1858 1887 Size: 551 831 332 96 48 29 Fragments arranged by size: 831 551 332 96 48 29 TatI w'GTAC_w Cuts at: 0 706 1887 Size: 706 1181 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1813 1845 1887 Size: 1813 32 42 Fragments arranged by size: 1813 42 32 Tth111I GACn'n_nGTC Cuts at: 0 13 1887 Size: 13 1874 XbaI T'CTAG_A Cuts at: 0 1416 1887 Size: 1416 471 XmaI C'CCGG_G Cuts at: 0 1213 1669 1887 Size: 1213 456 218 Fragments arranged by size: 1213 456 218 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BstXI BstYI BtgI BtgZI DraIII EaeI EagI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HpaI KasI MluI MmeI MscI MslI MspA1I NarI NruI NspI PasI PfoI PpuMI PshAI PspOMI PvuII SalI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AleI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BmrI BmtI BplI Bpu10I BsaAI BseYI BsiWI BsmI BspEI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EarI EcoICRI FalI FseI FspAI HindIII KpnI MfeI NaeI NcoI NdeI NgoMIV NheI NotI NsiI PacI PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XcmI XhoI XmnI ZraI