################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI AspEI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI Hin1I HindIII HinfI HpaI KasI KspI MamI MfeI MluI MroI MslI MspA1I MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI TfiI TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 614 GGTACC G^GTACC AcsI 438 AAATTT [GA]^AATT[CT] AlwI 452 GATCC GGATC(4/5) Aor51HI 589 AGCGCT AGC^GCT ApoI 438 AAATTT [GA]^AATT[CT] Asp700I 402 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 614 GGTACC G^GTACC AspHI 484 GAGCAC G[AT]GC[AT]^C AspI 627 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 201 GGACC G^G[AT]CC BbvI 511 GCTGC GCAGC(8/12) BfaI 523 CTAG C^TAG BmyI 484 GAGCAC G[AGT]GC[ACT]^C BpmI 371 CTCCAG CTGGAG(16/14) BsaJI 324 CCCCGG C^C[ACGT][ACGT]GG BsgI 356 GTGCAG GTGCAG(16/14) BsiHKAI 484 GAGCAC G[AT]GC[AT]^C BsmFI 200 GGGAC GGGAC(10/14) Bsp1286I 484 GAGCAC G[AGT]GC[ACT]^C BstNI 204 CCAGG CC^[AT]GG BstYI 451 AGATCC [GA]^GATC[CT] CfoI 590 GCGC GCG^C Cfr13I 201 GGACC G^G[ACGT]CC DdeI 32 CTAAG C^T[ACGT]AG DpnII 452 GATC ^GATC EarI 406 CTCTTC CTCTTC(1/4) Eco47III 589 AGCGCT AGC^GCT EcoRII 204 CCAGG ^CC[AT]GG HaeII 589 AGCGCT [GA]GCGC^[CT] HgaI 329 GACGC GACGC(5/10) HhaI 590 GCGC GCG^C HinP1I 590 GCGC G^CGC HphI 641 TCACC GGTGA(8/7) KpnI 614 GGTACC GGTAC^C Ksp632I 406 CTCTTC CTCTTC(1/4) MaeI 523 CTAG C^TAG MboI 452 GATC ^GATC MflI 451 AGATCC [GA]^GATC[CT] MvaI 204 CCAGG CC^[AT]GG NdeII 452 GATC ^GATC PflFI 627 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 122 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 405 GCTCTTC GCTCTTC(1/4) Sau3AI 452 GATC ^GATC Sau96I 201 GGACC G^G[ACGT]CC SfaNI 9 GCATC GCATC(5/9) SspI 173 AATATT AAT^ATT TseI 511 GCTGC G^C[AT]GC Tth111I 627 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 122 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 373 CCAGCCTTTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 451 AGATCC [GA]^GATC[CT] XmnI 402 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 367 CCGC CCGC(-3/-1) 229 GCGG AluI 404 AGCT AG^CT 521 AGCT BalI 98 TGGCCA TGG^CCA 414 TGGCCA BanI 313 GGCACC G^G[CT][GA]CC 614 GGTACC BcnI 325 CCCGG CC^[GC]GG 584 CCGGG BsmI 220 GAATGC GAATGC(1/-1) 663 GAATGC BspMI 3 GCAGGT ACCTGC(4/8) 358 GCAGGT BsrI 103 ACTGG ACTGG(1/-1) 570 CCAGT Cac8I 538 GCGAGC GC[ACGT]^[ACGT]GC 542 GCCTGC EaeI 98 TGGCCA [CT]^GGCC[GA] 414 TGGCCA Fnu4HI 366 GCCGC GC^[ACGT]GC 511 GCTGC HapII 326 CCGG C^CGG 584 CCGG HincII 277 GTCAAC GT[CT]^[GA]AC 624 GTTGAC HindII 277 GTCAAC GT[CT]^[GA]AC 624 GTTGAC HpaII 326 CCGG C^CGG 584 CCGG ItaI 366 GCCGC GC^[ACGT]GC 511 GCTGC MaeII 80 ACGT A^CGT 473 ACGT MluNI 98 TGGCCA TGG^CCA 414 TGGCCA MscI 98 TGGCCA TGG^CCA 414 TGGCCA MseI 305 TTAA T^TAA 551 TTAA MspI 326 CCGG C^CGG 584 CCGG NciI 325 CCCGG CC^[GC]GG 584 CCGGG Tru9I 305 TTAA T^TAA 551 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AfaI BsiYI BslI BssKI HaeIII MaeIII MboII MnlI NlaIV RsaI ScrFI Tsp45I Tsp509I TspRI