################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AocI ApaI AscI Asp718I AspEI AvrII BamHI BanII BbrPI BbvCI BcgI BfrI BglI BlnI BsaAI BsaWI BseAI BsiEI BsiWI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FseI HincII HindII HindIII HpaI KpnI KspI MaeII MfeI MluI MroI MspA1I MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AccII 48 CGCG CG^CG AcyI 1334 GGCGCC G[GA]^CG[CT]C Alw44I 1270 GTGCAC G^TGCAC AlwNI 362 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1049 AGCGCT AGC^GCT ApaLI 1270 GTGCAC G^TGCAC AseI 775 ATTAAT AT^TAAT AsnI 775 ATTAAT AT^TAAT AspI 631 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1772 TGCGCA TGC^GCA BbeI 1334 GGCGCC GGCGC^C BciVI 1299 GGATAC GTATCC(6/5) BclI 1179 TGATCA T^GATCA BfaI 1318 CTAG C^TAG BglII 1137 AGATCT A^GATCT BlpI 36 GCTGAGC GC^T[ACGT]AGC BpmI 182 CTGGAG CTGGAG(16/14) Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC BsaBI 1180 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1334 GGCGCC G[GA]^CG[CT]C BsgI 799 GTGCAG GTGCAG(16/14) BsmBI 1450 GAGACG CGTCTC(1/5) BsmFI 133 GGGAC GGGAC(10/14) Bsp1407I 967 TGTACA T^GTACA BspDI 562 ATCGAT AT^CGAT BspMI 801 GCAGGT ACCTGC(4/8) BsrBI 185 GAGCGG CCGCTC(-3/-3) BsrDI 1516 CATTGC GCAATG(2/0) BsrFI 1331 ACCGGC [GA]^CCGG[CT] BsrGI 967 TGTACA T^GTACA BstUI 48 CGCG CG^CG CelII 36 GCTGAGC GC^T[ACGT]AGC Cfr10I 1331 ACCGGC [GA]^CCGG[CT] ClaI 562 ATCGAT AT^CGAT DraI 1219 TTTAAA TTT^AAA DraII 587 AGGCCCC [GA]G^G[ACGT]CC[CT] EarI 319 GAAGAG CTCTTC(1/4) Eco47III 1049 AGCGCT AGC^GCT EcoO109I 587 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoT22I 686 ATGCAT ATGCA^T FauI 187 GCGGG CCCGC(4/6) FbaI 1179 TGATCA T^GATCA FspI 1772 TGCGCA TGC^GCA HgaI 332 GACGC GACGC(5/10) Hin1I 1334 GGCGCC G[GA]^CG[CT]C KasI 1334 GGCGCC G^GCGCC Ksp632I 319 GAAGAG CTCTTC(1/4) MaeI 1318 CTAG C^TAG MamI 1180 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 48 CGCG CG^CG NarI 1334 GGCGCC GG^CGCC NsiI 686 ATGCAT ATGCA^T PaeR7I 1784 CTCGAG C^TCGAG PflFI 631 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 775 ATTAAT AT^TAAT PstI 1418 CTGCAG CTGCA^G ScaI 857 AGTACT AGT^ACT SexAI 224 ACCTGGT A^CC[AT]GGT SfoI 1334 GGCGCC GGC^GCC SmlI 1784 CTCGAG C^T[CT][GA]AG SspBI 967 TGTACA T^GTACA SspI 268 AATATT AAT^ATT Tth111I 631 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1317 TCTAGA T^CTAGA XcmI 1353 CCACCCTCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1784 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Asp700I 1432 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1666 GAAATAGTTC BalI 1004 TGGCCA TGG^CCA 1463 TGGCCA BanI 1334 GGCGCC G^G[CT][GA]CC 1624 GGTGCC BsaI 1565 GAGACC GGTCTC(1/5) 280 GGTCTC BspHI 435 TCATGA T^CATGA 939 TCATGA BstAPI 340 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 792 GCAGTGGGTGC BstNI 225 CCTGG CC^[AT]GG 1648 CCTGG EaeI 1004 TGGCCA [CT]^GGCC[GA] 1463 TGGCCA EcoRII 225 CCTGG ^CC[AT]GG 1648 CCTGG HaeII 1049 AGCGCT [GA]GCGC^[CT] 1334 GGCGCC MaeIII 82 GTCAC ^GT[ACGT]AC 263 GTGAC MluNI 1004 TGGCCA TGG^CCA 1463 TGGCCA MscI 1004 TGGCCA TGG^CCA 1463 TGGCCA MslI 436 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 487 CATGTTTGTG MvaI 225 CCTGG CC^[AT]GG 1648 CCTGG RcaI 435 TCATGA T^CATGA 939 TCATGA SmaI 10 CCCGGG CCC^GGG 530 CCCGGG SphI 1413 GCATGC GCATG^C 1577 GCATGC TfiI 565 GATTC G^A[AT]TC 1443 GAATC Tsp45I 82 GTCAC ^GT[GC]AC 263 GTGAC XmaCI 10 CCCGGG C^CCGGG 530 CCCGGG XmaI 10 CCCGGG C^CCGGG 530 CCCGGG XmnI 1432 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1666 GAAATAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BbsI BbvI BcnI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboI MboII MflI MnlI MseI MspI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I Van91I XhoII