################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AvaI AvaII AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HgaI HindIII HpaI KspI MfeI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TspRI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 929 GGTACC G^GTACC AccII 1392 CGCG CG^CG AcyI 961 GGCGCC G[GA]^CG[CT]C AlwNI 503 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 627 CCTCAGG CC^T[ACGT]AGG ApaI 402 GGGCCC GGGCC^C Asp718I 929 GGTACC G^GTACC AspHI 817 GAGCAC G[AT]GC[AT]^C AspI 565 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 961 GGCGCC GGCGC^C BfaI 517 CTAG C^TAG BpmI 319 CTGGAG CTGGAG(16/14) BsaBI 34 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 961 GGCGCC G[GA]^CG[CT]C BsiHKAI 817 GAGCAC G[AT]GC[AT]^C Bsp1407I 1127 TGTACA T^GTACA BspMI 1065 GCAGGT ACCTGC(4/8) BsrBI 226 CCGCTC CCGCTC(-3/-3) BsrFI 412 GCCGGC [GA]^CCGG[CT] BsrGI 1127 TGTACA T^GTACA BssSI 1030 CTCGTG CACGAG(-5/-1) BstUI 1392 CGCG CG^CG Bsu36I 627 CCTCAGG CC^T[ACGT]AGG Cfr10I 412 GCCGGC [GA]^CCGG[CT] Eco81I 627 CCTCAGG CC^T[ACGT]AGG EcoNI 1398 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 187 GATATC GAT^ATC FauI 225 CCCGC CCCGC(4/6) HaeII 961 GGCGCC [GA]GCGC^[CT] Hin1I 961 GGCGCC G[GA]^CG[CT]C HincII 466 GTCAAC GT[CT]^[GA]AC HindII 466 GTCAAC GT[CT]^[GA]AC KasI 961 GGCGCC G^GCGCC KpnI 929 GGTACC GGTAC^C MaeI 517 CTAG C^TAG MamI 34 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 1392 CGCG CG^CG NaeI 412 GCCGGC GCC^GGC NarI 961 GGCGCC GG^CGCC NcoI 948 CCATGG C^CATGG NgoMIV 412 GCCGGC G^CCGGC PflFI 565 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1162 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1414 CAGCTG CAG^CTG SapI 1193 GAAGAGC GCTCTTC(1/4) SfoI 961 GGCGCC GGC^GCC SspBI 1127 TGTACA T^GTACA Tsp509I 586 AATT ^AATT Tth111I 565 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BbsI 273 GAAGAC GAAGAC(2/6) 1203 GAAGAC BglI 482 GCCTCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 626 GCCTCAGGGGC BpuAI 273 GAAGAC GAAGAC(2/6) 1203 GAAGAC BsaI 1155 GGTCTC GGTCTC(1/5) 1407 GGTCTC BsmBI 935 CGTCTC CGTCTC(1/5) 362 GAGACG BsmFI 332 GGGAC GGGAC(10/14) 942 GTCCC BsrI 180 ACTGG ACTGG(1/-1) 590 ACTGG BstYI 221 AGATCC [GA]^GATC[CT] 881 AGATCC CfoI 877 GCGC GCG^C 962 GCGC DraII 401 AGGGCCC [GA]G^G[ACGT]CC[CT] 512 AGGGCCT EcoO109I 401 AGGGCCC [GA]G^G[ACGT]CC[CT] 512 AGGGCCT EcoT14I 863 CCAAGG C^C[AT][AT]GG 948 CCATGG HhaI 877 GCGC GCG^C 962 GCGC HinP1I 877 GCGC G^CGC 962 GCGC MaeII 470 ACGT A^CGT 1292 ACGT MflI 221 AGATCC [GA]^GATC[CT] 881 AGATCC MspA1I 1316 CAGCGG C[AC]G^C[GT]G 1414 CAGCTG PleI 1044 GACTC GAGTC(4/5) 499 GAGTC PstI 146 CTGCAG CTGCA^G 311 CTGCAG SfcI 146 CTGCAG C^T[GA][CT]AG 311 CTGCAG StyI 863 CCAAGG C^C[AT][AT]GG 948 CCATGG XhoII 221 AGATCC [GA]^GATC[CT] 881 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AfaI AluI AlwI BanI BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I TthHB8I