(Linear) (Six-base) MAPSORT of: KIEE6479.seq Check: 8088 from: 1 to: 1425 KIEE6479.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. Acc65I G'GTAC_C Cuts at: 0 929 1425 Size: 929 496 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1070 1149 1425 Size: 1070 79 276 Fragments arranged by size: 1070 276 79 AleI CACnn'nnGTG Cuts at: 0 352 1425 Size: 352 1073 AlwNI CAG_nnn'CTG Cuts at: 0 508 1425 Size: 508 917 ApaI G_GGCC'C Cuts at: 0 406 1425 Size: 406 1019 BanI G'GyrC_C Cuts at: 0 293 929 961 1425 Size: 293 636 32 464 Fragments arranged by size: 636 464 293 32 BanII G_rGCy'C Cuts at: 0 172 259 344 406 703 745 1332 1425 Size: 172 87 85 62 297 42 587 93 Fragments arranged by size: 587 297 172 93 87 85 62 42 BbeI G_GCGC'C Cuts at: 0 965 1425 Size: 965 460 BbsI GAAGACnn'nnnn_ Cuts at: 0 280 1210 1425 Size: 280 930 215 Fragments arranged by size: 930 280 215 BglI GCCn_nnn'nGGC Cuts at: 0 488 632 1425 Size: 488 144 793 Fragments arranged by size: 793 488 144 Bme1580I G_kGCm'C Cuts at: 0 296 406 1425 Size: 296 110 1019 Fragments arranged by size: 1019 296 110 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 522 1425 Size: 522 903 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 1425 Size: 340 1085 BsaI GGTCTCn'nnnn_ Cuts at: 0 1161 1413 1425 Size: 1161 252 12 Fragments arranged by size: 1161 252 12 BsaBI GATnn'nnATC Cuts at: 0 38 1425 Size: 38 1387 BsaHI Gr'CG_yC Cuts at: 0 962 1425 Size: 962 463 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 188 218 1425 Size: 188 30 1207 Fragments arranged by size: 1207 188 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 511 829 868 1425 Size: 511 318 39 557 Fragments arranged by size: 557 511 318 39 BseYI C'CCAG_C Cuts at: 0 252 329 594 1186 1412 1425 Size: 252 77 265 592 226 13 Fragments arranged by size: 592 265 252 226 77 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 684 874 1425 Size: 325 359 190 551 Fragments arranged by size: 551 359 325 190 BsiHKAI G_wGCw'C Cuts at: 0 821 1425 Size: 821 604 BsmBI CGTCTCn'nnnn_ Cuts at: 0 356 941 1425 Size: 356 585 484 Fragments arranged by size: 585 484 356 Bsp1286I G_dGCh'C Cuts at: 0 172 259 296 344 406 703 745 821 Size: 172 87 37 48 62 297 42 76 Cuts at: 821 1332 1425 Size: 511 93 Fragments arranged by size: 511 297 172 93 87 76 62 48 42 37 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1056 1425 Size: 1056 369 BsrBI CCG'CTC Cuts at: 0 228 1425 Size: 228 1197 BsrFI r'CCGG_y Cuts at: 0 412 1425 Size: 412 1013 BsrGI T'GTAC_A Cuts at: 0 1127 1425 Size: 1127 298 BssSI C'ACGA_G Cuts at: 0 1030 1425 Size: 1030 395 BstXI CCAn_nnnn'nTGG Cuts at: 0 870 1005 1386 1425 Size: 870 135 381 39 Fragments arranged by size: 870 381 135 39 BstYI r'GATC_y Cuts at: 0 221 881 1425 Size: 221 660 544 Fragments arranged by size: 660 544 221 Bsu36I CC'TnA_GG Cuts at: 0 628 1425 Size: 628 797 BtgI C'CryG_G Cuts at: 0 794 948 1137 1280 1425 Size: 794 154 189 143 145 Fragments arranged by size: 794 189 154 145 143 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 56 91 1425 Size: 56 35 1334 Fragments arranged by size: 1334 56 35 EaeI y'GGCC_r Cuts at: 0 100 410 797 1388 1425 Size: 100 310 387 591 37 Fragments arranged by size: 591 387 310 100 37 EarI CTCTTCn'nnn_ Cuts at: 0 103 806 1188 1425 Size: 103 703 382 237 Fragments arranged by size: 703 382 237 103 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 1070 1149 1425 Size: 340 730 79 276 Fragments arranged by size: 730 340 276 79 EcoNI CCTnn'n_nnAGG Cuts at: 0 1402 1425 Size: 1402 23 EcoO109I rG'GnC_Cy Cuts at: 0 402 513 1425 Size: 402 111 912 Fragments arranged by size: 912 402 111 EcoRV GAT'ATC Cuts at: 0 189 1425 Size: 189 1236 HaeII r_GCGC'y Cuts at: 0 965 1425 Size: 965 460 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 104 136 467 499 1425 Size: 104 32 331 32 926 Fragments arranged by size: 926 331 104 32 32 HincII GTy'rAC Cuts at: 0 468 1425 Size: 468 957 KasI G'GCGC_C Cuts at: 0 961 1425 Size: 961 464 KpnI G_GTAC'C Cuts at: 0 933 1425 Size: 933 492 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 284 1184 1425 Size: 284 900 241 Fragments arranged by size: 900 284 241 MslI CAynn'nnrTG Cuts at: 0 299 352 655 913 1425 Size: 299 53 303 258 512 Fragments arranged by size: 512 303 299 258 53 MspA1I CmG'CkG Cuts at: 0 1318 1416 1425 Size: 1318 98 9 Fragments arranged by size: 1318 98 9 NaeI GCC'GGC Cuts at: 0 414 1425 Size: 414 1011 NarI GG'CG_CC Cuts at: 0 962 1425 Size: 962 463 NcoI C'CATG_G Cuts at: 0 948 1425 Size: 948 477 NgoMIV G'CCGG_C Cuts at: 0 412 1425 Size: 412 1013 PasI CC'CwG_GG Cuts at: 0 1151 1425 Size: 1151 274 PshAI GACnn'nnGTC Cuts at: 0 1166 1425 Size: 1166 259 PspOMI G'GGCC_C Cuts at: 0 402 1425 Size: 402 1023 PstI C_TGCA'G Cuts at: 0 150 315 1425 Size: 150 165 1110 Fragments arranged by size: 1110 165 150 PvuII CAG'CTG Cuts at: 0 1416 1425 Size: 1416 9 SapI GCTCTTCn'nnn_ Cuts at: 0 1188 1425 Size: 1188 237 SfcI C'TryA_G Cuts at: 0 146 311 1425 Size: 146 165 1114 Fragments arranged by size: 1114 165 146 SfoI GGC'GCC Cuts at: 0 963 1425 Size: 963 462 StyI C'CwwG_G Cuts at: 0 863 948 1425 Size: 863 85 477 Fragments arranged by size: 863 477 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 63 1425 Size: 63 1362 TatI w'GTAC_w Cuts at: 0 26 1127 1425 Size: 26 1101 298 Fragments arranged by size: 1101 298 26 Tth111I GACn'n_nGTC Cuts at: 0 568 1425 Size: 568 857 Enzymes that do cut: Acc65I AcuI AleI AlwNI ApaI BanI BanII BbeI BbsI BglI Bme1580I BplI BpmI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI CspCI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI KpnI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PshAI PspOMI PstI PvuII SapI SfcI SfoI StyI TaqII TatI Tth111I Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrDI BssHII BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoRI FalI FseI FspI FspAI HindIII HpaI MfeI MluI MscI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI