Restriction map for pFN21AE6479
 (Linear) (Six-base) MAPSORT of: KIEE6479.seq  Check: 8088  from: 1  to: 1425



KIEE6479.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

Acc65I G'GTAC_C

Cuts at:      0     929    1425 
   Size:        929     496
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1070    1149    1425 
   Size:       1070      79     276

  Fragments arranged by size: 

                1070     276      79
 
AleI CACnn'nnGTG

Cuts at:      0     352    1425 
   Size:        352    1073
 
AlwNI CAG_nnn'CTG

Cuts at:      0     508    1425 
   Size:        508     917
 
ApaI G_GGCC'C

Cuts at:      0     406    1425 
   Size:        406    1019
 
BanI G'GyrC_C

Cuts at:      0     293     929     961    1425 
   Size:        293     636      32     464

  Fragments arranged by size: 

                 636     464     293      32
 
BanII G_rGCy'C

Cuts at:      0     172     259     344     406     703     745    1332    1425 
   Size:        172      87      85      62     297      42     587      93

  Fragments arranged by size: 

                 587     297     172      93      87      85      62      42
            
 
BbeI G_GCGC'C

Cuts at:      0     965    1425 
   Size:        965     460
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     280    1210    1425 
   Size:        280     930     215

  Fragments arranged by size: 

                 930     280     215
 
BglI GCCn_nnn'nGGC

Cuts at:      0     488     632    1425 
   Size:        488     144     793

  Fragments arranged by size: 

                 793     488     144
 
Bme1580I G_kGCm'C

Cuts at:      0     296     406    1425 
   Size:        296     110    1019

  Fragments arranged by size: 

                1019     296     110
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     522    1425 
   Size:        522     903
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340    1425 
   Size:        340    1085
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1161    1413    1425 
   Size:       1161     252      12

  Fragments arranged by size: 

                1161     252      12
 
BsaBI GATnn'nnATC

Cuts at:      0      38    1425 
   Size:         38    1387
 
BsaHI Gr'CG_yC

Cuts at:      0     962    1425 
   Size:        962     463
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     188     218    1425 
   Size:        188      30    1207

  Fragments arranged by size: 

                1207     188      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     511     829     868    1425 
   Size:        511     318      39     557

  Fragments arranged by size: 

                 557     511     318      39
 
BseYI C'CCAG_C

Cuts at:      0     252     329     594    1186    1412    1425 
   Size:        252      77     265     592     226      13

  Fragments arranged by size: 

                 592     265     252     226      77      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     684     874    1425 
   Size:        325     359     190     551

  Fragments arranged by size: 

                 551     359     325     190
 
BsiHKAI G_wGCw'C

Cuts at:      0     821    1425 
   Size:        821     604
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     356     941    1425 
   Size:        356     585     484

  Fragments arranged by size: 

                 585     484     356
 
Bsp1286I G_dGCh'C

Cuts at:      0     172     259     296     344     406     703     745     821 
   Size:        172      87      37      48      62     297      42      76

Cuts at:    821    1332    1425 
   Size:        511      93

  Fragments arranged by size: 

                 511     297     172      93      87      76      62      48
                  42      37
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1056    1425 
   Size:       1056     369
 
BsrBI CCG'CTC

Cuts at:      0     228    1425 
   Size:        228    1197
 
BsrFI r'CCGG_y

Cuts at:      0     412    1425 
   Size:        412    1013
 
BsrGI T'GTAC_A

Cuts at:      0    1127    1425 
   Size:       1127     298
 
BssSI C'ACGA_G

Cuts at:      0    1030    1425 
   Size:       1030     395
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     870    1005    1386    1425 
   Size:        870     135     381      39

  Fragments arranged by size: 

                 870     381     135      39
 
BstYI r'GATC_y

Cuts at:      0     221     881    1425 
   Size:        221     660     544

  Fragments arranged by size: 

                 660     544     221
 
Bsu36I CC'TnA_GG

Cuts at:      0     628    1425 
   Size:        628     797
 
BtgI C'CryG_G

Cuts at:      0     794     948    1137    1280    1425 
   Size:        794     154     189     143     145

  Fragments arranged by size: 

                 794     189     154     145     143
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      56      91    1425 
   Size:         56      35    1334

  Fragments arranged by size: 

                1334      56      35
 
EaeI y'GGCC_r

Cuts at:      0     100     410     797    1388    1425 
   Size:        100     310     387     591      37

  Fragments arranged by size: 

                 591     387     310     100      37
 
EarI CTCTTCn'nnn_

Cuts at:      0     103     806    1188    1425 
   Size:        103     703     382     237

  Fragments arranged by size: 

                 703     382     237     103
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340    1070    1149    1425 
   Size:        340     730      79     276

  Fragments arranged by size: 

                 730     340     276      79
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1402    1425 
   Size:       1402      23
 
EcoO109I rG'GnC_Cy

Cuts at:      0     402     513    1425 
   Size:        402     111     912

  Fragments arranged by size: 

                 912     402     111
 
EcoRV GAT'ATC

Cuts at:      0     189    1425 
   Size:        189    1236
 
HaeII r_GCGC'y

Cuts at:      0     965    1425 
   Size:        965     460
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     467     499    1425 
   Size:        104      32     331      32     926

  Fragments arranged by size: 

                 926     331     104      32      32
 
HincII GTy'rAC

Cuts at:      0     468    1425 
   Size:        468     957
 
KasI G'GCGC_C

Cuts at:      0     961    1425 
   Size:        961     464
 
KpnI G_GTAC'C

Cuts at:      0     933    1425 
   Size:        933     492
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     284    1184    1425 
   Size:        284     900     241

  Fragments arranged by size: 

                 900     284     241
 
MslI CAynn'nnrTG

Cuts at:      0     299     352     655     913    1425 
   Size:        299      53     303     258     512

  Fragments arranged by size: 

                 512     303     299     258      53
 
MspA1I CmG'CkG

Cuts at:      0    1318    1416    1425 
   Size:       1318      98       9

  Fragments arranged by size: 

                1318      98       9
 
NaeI GCC'GGC

Cuts at:      0     414    1425 
   Size:        414    1011
 
NarI GG'CG_CC

Cuts at:      0     962    1425 
   Size:        962     463
 
NcoI C'CATG_G

Cuts at:      0     948    1425 
   Size:        948     477
 
NgoMIV G'CCGG_C

Cuts at:      0     412    1425 
   Size:        412    1013
 
PasI CC'CwG_GG

Cuts at:      0    1151    1425 
   Size:       1151     274
 
PshAI GACnn'nnGTC

Cuts at:      0    1166    1425 
   Size:       1166     259
 
PspOMI G'GGCC_C

Cuts at:      0     402    1425 
   Size:        402    1023
 
PstI C_TGCA'G

Cuts at:      0     150     315    1425 
   Size:        150     165    1110

  Fragments arranged by size: 

                1110     165     150
 
PvuII CAG'CTG

Cuts at:      0    1416    1425 
   Size:       1416       9
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1188    1425 
   Size:       1188     237
 
SfcI C'TryA_G

Cuts at:      0     146     311    1425 
   Size:        146     165    1114

  Fragments arranged by size: 

                1114     165     146
 
SfoI GGC'GCC

Cuts at:      0     963    1425 
   Size:        963     462
 
StyI C'CwwG_G

Cuts at:      0     863     948    1425 
   Size:        863      85     477

  Fragments arranged by size: 

                 863     477      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      63    1425 
   Size:         63    1362
 
TatI w'GTAC_w

Cuts at:      0      26    1127    1425 
   Size:         26    1101     298

  Fragments arranged by size: 

                1101     298      26
 
Tth111I GACn'n_nGTC

Cuts at:      0     568    1425 
   Size:        568     857
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI    AlwNI     ApaI     BanI    BanII     BbeI
     BbsI     BglI Bme1580I     BplI     BpmI     BsaI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrFI    BsrGI    BssSI    BstXI    BstYI   Bsu36I     BtgI
    CspCI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII
    Hin4I   HincII     KasI     KpnI     MmeI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     PasI    PshAI   PspOMI     PstI    PvuII
     SapI     SfcI     SfoI     StyI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI   Bpu10I    BpuEI
    BsaAI    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrDI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI     FalI
     FseI     FspI    FspAI  HindIII     HpaI     MfeI     MluI     MscI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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