################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MaeII MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 368 GTCGAC GT^[AC][GT]AC AciI 283 GCGG CCGC(-3/-1) AcsI 157 GAATTT [GA]^AATT[CT] ApoI 157 GAATTT [GA]^AATT[CT] AseI 315 ATTAAT AT^TAAT AsnI 315 ATTAAT AT^TAAT AspHI 443 GAGCAC G[AT]GC[AT]^C BamHI 63 GGATCC G^GATCC BbsI 378 GAAGAC GAAGAC(2/6) BglII 456 AGATCT A^GATCT BmyI 443 GAGCAC G[AGT]GC[ACT]^C BpmI 373 CTCCAG CTGGAG(16/14) BpuAI 378 GAAGAC GAAGAC(2/6) BsaBI 205 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 443 GAGCAC G[AT]GC[AT]^C Bsp1286I 443 GAGCAC G[AGT]GC[ACT]^C BssKI 72 CCAGG ^CC[ACGT]GG BstNI 72 CCAGG CC^[AT]GG BstXI 375 CCAGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 18 GCTTGC GC[ACGT]^[ACGT]GC EcoRII 72 CCAGG ^CC[AT]GG HaeIII 257 GGCC GG^CC HincII 368 GTCGAC GT[CT]^[GA]AC HindII 368 GTCGAC GT[CT]^[GA]AC HindIII 118 AAGCTT A^AGCTT HphI 403 TCACC GGTGA(8/7) MamI 205 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 316 TTAA T^TAA MvaI 72 CCAGG CC^[AT]GG MwoI 111 GCGAGAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 9 CATG CATG^ NlaIV 63 GGATCC GG[ACGT]^[ACGT]CC PshBI 315 ATTAAT AT^TAAT SalI 368 GTCGAC G^TCGAC ScrFI 72 CCAGG CC^[ACGT]GG SmlI 464 CTCAAG C^T[CT][GA]AG TaqI 369 TCGA T^CGA Tru9I 316 TTAA T^TAA Tsp45I 12 GTCAC ^GT[GC]AC TspRI 132 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 369 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AluI 17 AGCT AG^CT 119 AGCT AlwI 64 GATCC GGATC(4/5) 63 GGATC BsrI 224 ACTGG ACTGG(1/-1) 382 ACTGG BstF5I 147 GGATG GGATG(2/0) 438 GGATG BstYI 63 GGATCC [GA]^GATC[CT] 456 AGATCT DdeI 121 CTTAG C^T[ACGT]AG 164 CTAAG DpnII 64 GATC ^GATC 457 GATC FokI 147 GGATG GGATG(9/13) 438 GGATG MaeIII 12 GTCAC ^GT[ACGT]AC 394 GTAAC MboI 64 GATC ^GATC 457 GATC MboII 310 GAAGA GAAGA(8/7) 378 GAAGA MflI 63 GGATCC [GA]^GATC[CT] 456 AGATCT NdeII 64 GATC ^GATC 457 GATC PleI 371 GACTC GAGTC(4/5) 462 GACTC Sau3AI 64 GATC ^GATC 457 GATC TfiI 138 GATTC G^A[AT]TC 228 GAATC XhoII 63 GGATCC [GA]^GATC[CT] 456 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### HinfI MnlI Tsp509I