################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaI BsiEI BsiWI BsoBI BspDI BspHI BspMI BsrDI BssHII BstAPI BstBI BstXI CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 1408 GACGTC GACGT^C AccII 1215 CGCG CG^CG AccIII 875 TCCGGA T^CCGGA AcsI 552 GAATTC [GA]^AATT[CT] AflIII 110 ACATGT A^C[GA][CT]GT AlwNI 777 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 760 CCTGAGG CC^T[ACGT]AGG ApaI 224 GGGCCC GGGCC^C ApoI 552 GAATTC [GA]^AATT[CT] AspHI 424 GAGCTC G[AT]GC[AT]^C BamHI 878 GGATCC G^GATCC BanI 1312 GGCACC G^G[CT][GA]CC BbsI 1384 GTCTTC GAAGAC(2/6) BbvCI 721 CCTCAGC CCTCAGC(-5/-2) BclI 1181 TGATCA T^GATCA BglII 1451 AGATCT A^GATCT BpmI 45 CTCCAG CTGGAG(16/14) BpuAI 1384 GTCTTC GAAGAC(2/6) BsaBI 1176 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 875 TCCGGA [AT]^CCGG[AT] BseAI 875 TCCGGA T^CCGGA BseRI 719 CTCCTC GAGGAG(10/8) BsiHKAI 424 GAGCTC G[AT]GC[AT]^C BsmBI 1059 CGTCTC CGTCTC(1/5) BsmI 362 GAATGC GAATGC(1/-1) Bsp1407I 1133 TGTACA T^GTACA BspEI 875 TCCGGA T^CCGGA BspLU11I 110 ACATGT A^CATGT BsrGI 1133 TGTACA T^GTACA BssSI 421 CACGAG CACGAG(-5/-1) Bst1107I 1441 GTATAC GTA^TAC BstEII 492 GGTGACC G^GT[ACGT]ACC BstPI 492 GGTGACC G^GT[ACGT]ACC BstUI 1215 CGCG CG^CG BstZ17I 1441 GTATAC GTA^TAC Bsu36I 760 CCTGAGG CC^T[ACGT]AGG DraIII 207 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 760 CCTGAGG CC^T[ACGT]AGG EcoNI 41 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 492 GGTGACC G^GT[ACGT]ACC EcoRI 552 GAATTC G^AATTC FauI 528 GCGGG CCCGC(4/6) FbaI 1181 TGATCA T^GATCA HaeII 870 GGCGCT [GA]GCGC^[CT] HgaI 665 GCGTC GACGC(5/10) MaeII 1409 ACGT A^CGT MamI 1176 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 875 TCCGGA T^CCGGA MvnI 1215 CGCG CG^CG NaeI 595 GCCGGC GCC^GGC NgoMIV 595 GCCGGC G^CCGGC SacI 424 GAGCTC GAGCT^C SapI 841 GAAGAGC GCTCTTC(1/4) SmlI 805 CTTGAG C^T[CT][GA]AG SspBI 1133 TGTACA T^GTACA StuI 66 AGGCCT AGG^CCT XcmI 447 CCAGGTCTGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 1008 GTCTAC GT^[AC][GT]AC 1441 GTATAC AcyI 664 GGCGTC G[GA]^CG[CT]C 1408 GACGTC AfaI 432 GTAC GT^AC 1134 GTAC AlwI 879 GATCC GGATC(4/5) 878 GGATC BalI 815 TGGCCA TGG^CCA 1146 TGGCCA BcgI 840 CGAAGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 506 GCAACTGGTTCG BcnI 19 CCGGG CC^[GC]GG 36 CCGGG BsaHI 664 GGCGTC G[GA]^CG[CT]C 1408 GACGTC BsgI 148 CTGCAC GTGCAG(16/14) 216 GTGCAG BsrI 509 ACTGG ACTGG(1/-1) 947 ACTGG BstYI 878 GGATCC [GA]^GATC[CT] 1451 AGATCT EcoT14I 827 CCAAGG C^C[AT][AT]GG 989 CCAAGG Hin1I 664 GGCGTC G[GA]^CG[CT]C 1408 GACGTC MflI 878 GGATCC [GA]^GATC[CT] 1451 AGATCT MluNI 815 TGGCCA TGG^CCA 1146 TGGCCA MscI 815 TGGCCA TGG^CCA 1146 TGGCCA NciI 19 CCGGG CC^[GC]GG 36 CCGGG PflMI 473 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1367 CCACGGGCTGG PpuMI 764 AGGACCC [GA]G^G[AT]CC[CT] 1001 GGGACCT RsaI 432 GTAC GT^AC 1134 GTAC StyI 827 CCAAGG C^C[AT][AT]GG 989 CCAAGG Tsp45I 493 GTGAC ^GT[GC]AC 1294 GTCAC Tsp509I 93 AATT ^AATT 553 AATT Van91I 473 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1367 CCACGGGCTGG XhoII 878 GGATCC [GA]^GATC[CT] 1451 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BfaI BglI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrFI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PvuII Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I