Restriction map for
pFN21AE5566
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaI AviII AvrII BalI BanII
BbeI BbrPI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
CpoI DraIII DrdI DsaI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I FbaI FseI FspI
HaeII HindIII KasI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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Acc65I 224 GGTACC G^GTACC
AccI 875 GTAGAC GT^[AC][GT]AC
AciI 73 GCGG CCGC(-3/-1)
AcsI 933 GAATTT [GA]^AATT[CT]
AcyI 3 GGCGTC G[GA]^CG[CT]C
AlwNI 336 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 933 GAATTT [GA]^AATT[CT]
Asp718I 224 GGTACC G^GTACC
BamHI 531 GGATCC G^GATCC
BanI 224 GGTACC G^G[CT][GA]CC
BbsI 181 GAAGAC GAAGAC(2/6)
BbvI 84 GCTGC GCAGC(8/12)
BpuAI 181 GAAGAC GAAGAC(2/6)
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaWI 38 ACCGGA [AT]^CCGG[AT]
BseRI 64 GAGGAG GAGGAG(10/8)
BsmAI 338 GAGAC GTCTC(1/5)
BsmI 909 GCATTC GAATGC(1/-1)
Bsp1407I 754 TGTACA T^GTACA
BspDI 407 ATCGAT AT^CGAT
BspHI 1121 TCATGA T^CATGA
BsrGI 754 TGTACA T^GTACA
BsrI 872 CCAGT ACTGG(1/-1)
BstAPI 371 GCATGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 416 GGATG GGATG(2/0)
BstXI 214 CCAATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1299 GCGC GCG^C
ClaI 407 ATCGAT AT^CGAT
DdeI 1069 CTTAG C^T[ACGT]AG
DraI 307 TTTAAA TTT^AAA
EaeI 59 CGGCCG [CT]^GGCC[GA]
EagI 59 CGGCCG C^GGCCG
EclXI 59 CGGCCG C^GGCCG
Eco52I 59 CGGCCG C^GGCCG
EcoT22I 907 ATGCAT ATGCA^T
FauI 73 GCGGG CCCGC(4/6)
Fnu4HI 84 GCTGC GC^[ACGT]GC
FokI 416 GGATG GGATG(9/13)
HgaI 4 GCGTC GACGC(5/10)
HhaI 1299 GCGC GCG^C
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HinP1I 1299 GCGC G^CGC
HincII 748 GTTAAC GT[CT]^[GA]AC
HindII 748 GTTAAC GT[CT]^[GA]AC
HinfI 1075 GATTC G^A[ACGT]TC
HpaI 748 GTTAAC GTT^AAC
ItaI 84 GCTGC GC^[ACGT]GC
KpnI 224 GGTACC GGTAC^C
MaeII 592 ACGT A^CGT
MslI 289 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 907 ATGCAT ATGCA^T
PflMI 1079 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 150 CTGCAG CTGCA^G
RcaI 1121 TCATGA T^CATGA
SfaNI 1065 GATGC GCATC(5/9)
SmlI 265 CTTGAG C^T[CT][GA]AG
SphI 686 GCATGC GCATG^C
SspBI 754 TGTACA T^GTACA
SspI 612 AATATT AAT^ATT
TfiI 1075 GATTC G^A[AT]TC
TseI 84 GCTGC G^C[AT]GC
TspRI 1302 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 1079 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BcgI 63 CGAGGAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1243 GCAGACCTCTCG
BsaJI 62 CCGAGG C^C[ACGT][ACGT]GG
620 CCTGGG
BsgI 25 GTGCAG GTGCAG(16/14)
344 GTGCAG
BsiEI 36 CGACCG CG[GA][CT]^CG
59 CGGCCG
BsmFI 210 GGGAC GGGAC(10/14)
158 GTCCC
BssKI 467 CCTGG ^CC[ACGT]GG
620 CCTGG
BstNI 467 CCTGG CC^[AT]GG
620 CCTGG
BstYI 240 AGATCC [GA]^GATC[CT]
531 GGATCC
DraII 156 GGGTCCC [GA]G^G[ACGT]CC[CT]
477 GGGTCCT
EcoO109I 156 GGGTCCC [GA]G^G[ACGT]CC[CT]
477 GGGTCCT
EcoRII 467 CCTGG ^CC[AT]GG
620 CCTGG
HapII 39 CCGG C^CGG
996 CCGG
HpaII 39 CCGG C^CGG
996 CCGG
MflI 240 AGATCC [GA]^GATC[CT]
531 GGATCC
MspI 39 CCGG C^CGG
996 CCGG
MvaI 467 CCTGG CC^[AT]GG
620 CCTGG
PpuMI 156 GGGTCCC [GA]G^G[AT]CC[CT]
477 GGGTCCT
ScrFI 467 CCTGG CC^[ACGT]GG
620 CCTGG
SfcI 150 CTGCAG C^T[GA][CT]AG
785 CTACAG
XhoII 240 AGATCC [GA]^GATC[CT]
531 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI AlwI AvaII BfaI
BsiYI BslI Cac8I Cfr13I DpnII
EarI HaeIII HphI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MwoI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I TaqI Tru9I
Tsp45I Tsp509I TthHB8I