(Circular) (Six-base) MAPSORT of: pFN21AE5566.seq Check: 284 from: 1 to: 6022 pFN21AE5566 6022 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3832 278 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2881 3365 1937 Size: 944 484 4594 Fragments arranged by size: 4594 944 484 Acc65I G'GTAC_C Cuts at: 2229 3343 2229 Size: 1114 4908 AclI AA'CG_TT Cuts at: 1563 4149 4522 1563 Size: 2586 373 3063 Fragments arranged by size: 3063 2586 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2487 2726 4085 5469 5737 1632 Size: 855 239 1359 1384 268 1917 Fragments arranged by size: 1917 1384 1359 855 268 239 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5825 AflIII A'CryG_T Cuts at: 1203 4921 5898 1203 Size: 3718 977 1327 Fragments arranged by size: 3718 1327 977 AgeI A'CCGG_T Cuts at: 4853 4853 Size: 6022 AhdI GACnn_n'nnGTC Cuts at: 4751 4751 Size: 6022 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6022 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6022 AlwNI CAG_nnn'CTG Cuts at: 2346 3765 5337 2346 Size: 1419 1572 3031 Fragments arranged by size: 3031 1572 1419 ApaLI G'TGCA_C Cuts at: 4079 5235 4079 Size: 1156 4866 ApoI r'AATT_y Cuts at: 1493 2938 3331 3557 3709 5818 1493 Size: 1445 393 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 393 226 152 AseI AT'TA_AT Cuts at: 160 1032 4576 160 Size: 872 3544 1606 Fragments arranged by size: 3544 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 AvaI C'yCGr_G Cuts at: 1870 1943 3347 1870 Size: 73 1404 4545 Fragments arranged by size: 4545 1404 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2221 2254 2221 Size: 33 5989 BamHI G'GATC_C Cuts at: 2536 3352 3719 2536 Size: 816 367 4839 Fragments arranged by size: 4839 816 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2229 3343 4798 618 Size: 325 217 627 442 1114 1455 1842 Fragments arranged by size: 1842 1455 1114 627 442 325 217 BanII G_rGCy'C Cuts at: 1391 3341 1391 Size: 1950 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2193 928 Size: 971 294 4757 Fragments arranged by size: 4757 971 294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2057 2091 3237 3271 4213 4247 1903 Size: 34 120 34 1146 34 942 34 3678 Fragments arranged by size: 3678 1146 942 120 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3914 5124 3914 Size: 1210 4812 BclI T'GATC_A Cuts at: 1548 1548 Size: 6022 BfrBI ATG'CAT Cuts at: 2914 2914 Size: 6022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4633 136 Size: 107 122 71 631 605 2961 1525 Fragments arranged by size: 2961 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6017 6017 Size: 6022 BlpI GC'TnA_GC Cuts at: 3439 3439 Size: 6022 Bme1580I G_kGCm'C Cuts at: 1790 4083 5239 1790 Size: 2293 1156 2573 Fragments arranged by size: 2573 2293 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6022 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3753 4711 442 Size: 416 53 83 399 24 2336 958 1753 Fragments arranged by size: 2336 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4682 1239 Size: 3443 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2291 4147 5012 5310 2291 Size: 1856 865 298 3003 Fragments arranged by size: 3003 1856 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4685 882 Size: 617 3186 2219 Fragments arranged by size: 3186 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3813 493 Size: 711 2609 2702 Fragments arranged by size: 2702 2609 711 BsaBI GATnn'nnATC Cuts at: 6016 6016 Size: 6022 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3829 4211 4846 Size: 53 83 186 936 476 1820 382 635 Cuts at: 4846 275 Size: 1451 Fragments arranged by size: 1820 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2043 4455 4853 5127 5274 5685 2043 Size: 2412 398 274 147 411 2380 Fragments arranged by size: 2412 2380 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5797 Fragments arranged by size: 5797 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 1186 Size: 898 5124 BseYI C'CCAG_C Cuts at: 2965 3299 5225 5721 2965 Size: 334 1926 496 3266 Fragments arranged by size: 3266 1926 496 334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 2370 1163 Size: 573 315 319 4815 Fragments arranged by size: 4815 573 319 315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2044 2067 3472 4233 5261 1540 Size: 275 186 43 23 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 43 23 BsiHKAI G_wGCw'C Cuts at: 4083 4168 5239 4083 Size: 85 1071 4866 Fragments arranged by size: 4866 1071 85 BsmI GAATG_Cn' Cuts at: 2914 3533 3626 2914 Size: 619 93 5310 Fragments arranged by size: 5310 619 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3341 4083 4168 5239 1391 Size: 399 1551 742 85 1071 2174 Fragments arranged by size: 2174 1551 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5685 5685 Size: 6022 BspHI T'CATG_A Cuts at: 3126 3911 5641 3126 Size: 785 1730 3507 Fragments arranged by size: 3507 1730 785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3377 844 Size: 2533 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3909 1394 Size: 429 2086 3507 Fragments arranged by size: 3507 2086 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4517 4691 65 Size: 1183 3269 174 1396 Fragments arranged by size: 3269 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4666 4853 1700 Size: 110 2856 187 2869 Fragments arranged by size: 2869 2856 187 110 BsrGI T'GTAC_A Cuts at: 96 2759 96 Size: 2663 3359 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6022 BssSI C'ACGA_G Cuts at: 4082 5094 4082 Size: 1012 5010 BstAPI GCAn_nnn'nTGC Cuts at: 794 2382 794 Size: 1588 4434 BstBI TT'CG_AA Cuts at: 4826 4826 Size: 6022 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6022 BstXI CCAn_nnnn'nTGG Cuts at: 2226 2226 Size: 6022 BstYI r'GATC_y Cuts at: 1972 2245 2536 3352 3719 4104 4121 5573 5659 Size: 273 291 816 367 385 17 1452 86 Cuts at: 5659 5671 6017 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 816 385 367 346 291 273 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4531 Fragments arranged by size: 4531 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5283 Fragments arranged by size: 5283 683 56 BtsI GCAGTG_nn' Cuts at: 3313 3538 4332 4352 3313 Size: 225 794 20 4983 Fragments arranged by size: 4983 794 225 20 ClaI AT'CG_AT Cuts at: 1554 2413 1554 Size: 859 5163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5987 DraI TTT'AAA Cuts at: 2314 3326 2314 Size: 1012 5010 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5029 809 Size: 507 3713 1802 Fragments arranged by size: 3713 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 3469 4358 Size: 54 1425 50 275 18 234 1405 889 Cuts at: 4358 5987 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1405 889 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2064 3469 1537 Size: 275 252 1405 4090 Fragments arranged by size: 4090 1405 275 252 EarI CTCTTCn'nnn_ Cuts at: 1353 2526 2666 2745 3055 3952 1353 Size: 1173 140 79 310 897 3423 Fragments arranged by size: 3423 1173 897 310 140 79 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4609 4983 5129 387 Size: 1064 138 3020 374 146 1280 Fragments arranged by size: 3020 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2487 2726 4085 4682 5469 5737 1239 Size: 393 855 239 1359 597 787 268 1524 Fragments arranged by size: 1524 1359 855 787 597 393 268 239 EcoO109I rG'GnC_Cy Cuts at: 2162 2483 2162 Size: 321 5701 EcoRI G'AATT_C Cuts at: 3331 3709 3331 Size: 378 5644 FspI TGC'GCA Cuts at: 4528 4528 Size: 6022 HaeII r_GCGC'y Cuts at: 1678 5169 1678 Size: 3491 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2995 3027 3097 3129 4663 Size: 32 135 32 1423 32 70 32 1534 Cuts at: 4663 4695 4737 4769 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1534 1423 135 70 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2755 3366 3612 669 Size: 988 281 817 611 246 3079 Fragments arranged by size: 3079 988 817 611 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6022 HpaI GTT'AAC Cuts at: 2755 3612 2755 Size: 857 5165 KpnI G_GTAC'C Cuts at: 2233 3347 2233 Size: 1114 4908 MfeI C'AATT_G Cuts at: 3621 3621 Size: 6022 MluI A'CGCG_T Cuts at: 5898 5898 Size: 6022 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1992 2384 5136 5320 1305 Size: 687 392 2752 184 2007 Fragments arranged by size: 2752 2007 687 392 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4200 Fragments arranged by size: 4200 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2298 3980 4339 4498 518 Size: 648 1132 1682 359 159 2042 Fragments arranged by size: 2042 1682 1132 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3438 4115 5263 5508 1606 Size: 1832 677 1148 245 2120 Fragments arranged by size: 2120 1832 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4531 Fragments arranged by size: 4531 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6022 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6022 NotI GC'GGCC_GC Cuts at: 3469 3469 Size: 6022 NsiI A_TGCA'T Cuts at: 2916 2916 Size: 6022 NspI r_CATG'y Cuts at: 1132 1229 2194 2207 2695 3380 4925 1132 Size: 97 965 13 488 685 1545 2229 Fragments arranged by size: 2229 1545 965 685 488 97 13 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6022 PciI A'CATG_T Cuts at: 4921 4921 Size: 6022 PflMI CCAn_nnn'nTGG Cuts at: 1108 3090 1108 Size: 1982 4040 PmeI GTTT'AAAC Cuts at: 3326 3326 Size: 6022 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4091 4123 5604 5636 4091 Size: 32 1481 32 4477 Fragments arranged by size: 4477 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2162 2483 2162 Size: 321 5701 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6022 PsiI TTA'TAA Cuts at: 2580 3592 2580 Size: 1012 5010 PstI C_TGCA'G Cuts at: 830 2159 3374 830 Size: 1329 1215 3478 Fragments arranged by size: 3478 1329 1215 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6022 SalI G'TCGA_C Cuts at: 1936 3364 1936 Size: 1428 4594 SanDI GG'GwC_CC Cuts at: 2162 2162 Size: 6022 SbfI CC_TGCA'GG Cuts at: 3374 3374 Size: 6022 ScaI AGT'ACT Cuts at: 4270 5982 4270 Size: 1712 4310 SfcI C'TryA_G Cuts at: 826 1045 2155 2790 3370 4505 5186 5377 826 Size: 219 1110 635 580 1135 681 191 1471 Fragments arranged by size: 1471 1135 1110 681 635 580 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6022 SmaI CCC'GGG Cuts at: 1872 3349 1872 Size: 1477 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2270 4126 5027 5289 820 Size: 197 926 327 1856 901 262 1553 Fragments arranged by size: 1856 1553 926 901 327 262 197 SnaBI TAC'GTA Cuts at: 493 3813 493 Size: 3320 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6022 SphI G_CATG'C Cuts at: 1132 2695 3380 1132 Size: 1563 685 3774 Fragments arranged by size: 3774 1563 685 SspI AAT'ATT Cuts at: 5 52 2619 3946 5 Size: 47 2567 1327 2081 Fragments arranged by size: 2567 2081 1327 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6022 StyI C'CwwG_G Cuts at: 513 1065 2004 3461 513 Size: 552 939 1457 3074 Fragments arranged by size: 3074 1457 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4049 4066 4219 4404 4849 325 Size: 1255 2469 17 153 185 445 1498 Fragments arranged by size: 2469 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2759 4268 5980 Size: 274 80 33 51 1443 782 1509 1712 Cuts at: 5980 96 Size: 138 Fragments arranged by size: 1712 1509 1443 782 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5982 6014 5982 Size: 32 5990 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6022 XbaI T'CTAG_A Cuts at: 3358 3358 Size: 6022 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6022 XmaI C'CCGG_G Cuts at: 1870 3347 1870 Size: 1477 4545 XmnI GAAnn'nnTTC Cuts at: 4151 4151 Size: 6022 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3830 276 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI