################################## # NO CUT ENZYMES ( 212 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I CpoI DdeI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AciI 64 GCGG CCGC(-3/-1) AcsI 201 GAATTT [GA]^AATT[CT] AfaI 211 GTAC GT^AC AlwI 90 GGATC GGATC(4/5) ApoI 201 GAATTT [GA]^AATT[CT] BanI 66 GGTGCC G^G[CT][GA]CC BbvI 132 GCAGC GCAGC(8/12) BglI 100 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsmAI 240 GTCTC GTCTC(1/5) BspDI 126 ATCGAT AT^CGAT BssKI 148 CCTGG ^CC[ACGT]GG BstNI 148 CCTGG CC^[AT]GG ClaI 126 ATCGAT AT^CGAT DsaI 216 CCGTGG C^C[GA][CT]GG EcoRII 148 CCTGG ^CC[AT]GG Fnu4HI 132 GCAGC GC^[ACGT]GC HaeIII 39 GGCC GG^CC HinfI 144 GAATC G^A[ACGT]TC ItaI 132 GCAGC GC^[ACGT]GC MboII 261 GAAGA GAAGA(8/7) MnlI 198 GAGG CCTC(7/6) MseI 269 TTAA T^TAA MvaI 148 CCTGG CC^[AT]GG MwoI 100 GCCTGTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC RsaI 211 GTAC GT^AC ScrFI 148 CCTGG CC^[ACGT]GG StuI 38 AGGCCT AGG^CCT TaqI 127 TCGA T^CGA TfiI 144 GAATC G^A[AT]TC Tru9I 269 TTAA T^TAA TseI 132 GCAGC G^C[AT]GC Tsp509I 202 AATT ^AATT TthHB8I 127 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BsaJI 148 CCTGGG C^C[ACGT][ACGT]GG 216 CCGTGG BsrI 188 ACTGG ACTGG(1/-1) 159 CCAGT DpnII 91 GATC ^GATC 264 GATC HphI 21 GGTGA GGTGA(8/7) 220 GGTGA MboI 91 GATC ^GATC 264 GATC MslI 11 CACAAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 214 CACCGTGGTG NdeII 91 GATC ^GATC 264 GATC NlaIV 66 GGTGCC GG[ACGT]^[ACGT]CC 155 GGAGCC Sau3AI 91 GATC ^GATC 264 GATC SfaNI 129 GATGC GCATC(5/9) 124 GCATC TspRI 77 CAGTG CA[GC]TG[ACGT][ACGT]^ 187 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MaeIII Tsp45I