(Circular) (Six-base) MAPSORT of: pFN21AE6176.seq Check: 5125 from: 1 to: 5035 pFN21AE6176 5035 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2845 278 Size: 53 83 186 936 1309 2468 Fragments arranged by size: 2468 1309 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2378 1937 Size: 441 4594 Acc65I G'GTAC_C Cuts at: 2356 2356 Size: 5035 AclI AA'CG_TT Cuts at: 1563 3162 3535 1563 Size: 1599 373 3063 Fragments arranged by size: 3063 1599 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3098 4482 4750 1632 Size: 1466 1384 268 1917 Fragments arranged by size: 1917 1466 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4838 AflIII A'CryG_T Cuts at: 1203 3934 4911 1203 Size: 2731 977 1327 Fragments arranged by size: 2731 1327 977 AgeI A'CCGG_T Cuts at: 3866 3866 Size: 5035 AhdI GACnn_n'nnGTC Cuts at: 3764 3764 Size: 5035 AleI CACnn'nnGTG Cuts at: 1166 2020 2223 1166 Size: 854 203 3978 Fragments arranged by size: 3978 854 203 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5035 AlwNI CAG_nnn'CTG Cuts at: 2778 4350 2778 Size: 1572 3463 ApaLI G'TGCA_C Cuts at: 3092 4248 3092 Size: 1156 3879 ApoI r'AATT_y Cuts at: 1493 2206 2344 2570 2722 4831 1493 Size: 713 138 226 152 2109 1697 Fragments arranged by size: 2109 1697 713 226 152 138 AseI AT'TA_AT Cuts at: 160 1032 3589 160 Size: 872 2557 1606 Fragments arranged by size: 2557 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5035 AvaI C'yCGr_G Cuts at: 1870 1943 2360 1870 Size: 73 417 4545 Fragments arranged by size: 4545 417 73 BamHI G'GATC_C Cuts at: 2365 2732 2365 Size: 367 4668 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2071 2356 3811 618 Size: 325 217 627 284 285 1455 1842 Fragments arranged by size: 1842 1455 627 325 285 284 217 BanII G_rGCy'C Cuts at: 1391 2354 1391 Size: 963 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4064 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3226 3260 1903 Size: 34 1289 34 3678 Fragments arranged by size: 3678 1289 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2927 4137 2927 Size: 1210 3825 BclI T'GATC_A Cuts at: 1548 1548 Size: 5035 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 3646 136 Size: 107 122 71 631 605 439 1535 1525 Fragments arranged by size: 1535 1525 631 605 439 122 107 71 BglII A'GATC_T Cuts at: 5030 5030 Size: 5035 BlpI GC'TnA_GC Cuts at: 2452 2452 Size: 5035 Bme1580I G_kGCm'C Cuts at: 1790 3096 4252 1790 Size: 1306 1156 2573 Fragments arranged by size: 2573 1306 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5035 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2766 3724 442 Size: 416 53 83 399 24 1349 958 1753 Fragments arranged by size: 1753 1349 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5035 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3695 1239 Size: 2456 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3160 4025 4323 3160 Size: 865 298 3872 Fragments arranged by size: 3872 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3698 882 Size: 617 2199 2219 Fragments arranged by size: 2219 2199 617 BsaAI yAC'GTr Cuts at: 493 1204 2826 493 Size: 711 1622 2702 Fragments arranged by size: 2702 1622 711 BsaBI GATnn'nnATC Cuts at: 5029 5029 Size: 5035 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2842 3224 3859 275 Size: 53 83 186 936 1309 382 635 1451 Fragments arranged by size: 1451 1309 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3468 3866 4140 4287 4698 3468 Size: 398 274 147 411 3805 Fragments arranged by size: 3805 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2289 2319 1178 Size: 30 165 30 886 30 3894 Fragments arranged by size: 3894 886 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5035 BseYI C'CCAG_C Cuts at: 4238 4734 4238 Size: 496 4539 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2485 3246 4274 1540 Size: 275 186 484 761 1028 2301 Fragments arranged by size: 2301 1028 761 484 275 186 BsiHKAI G_wGCw'C Cuts at: 3096 3181 4252 3096 Size: 85 1071 3879 Fragments arranged by size: 3879 1071 85 BsmI GAATG_Cn' Cuts at: 2546 2639 2546 Size: 93 4942 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2354 3096 3181 4252 1391 Size: 399 564 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 564 399 85 BspEI T'CCGG_A Cuts at: 4698 4698 Size: 5035 BspHI T'CATG_A Cuts at: 2924 4654 2924 Size: 1730 3305 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2390 844 Size: 1546 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2922 1394 Size: 429 1099 3507 Fragments arranged by size: 3507 1099 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3530 3704 65 Size: 1183 2282 174 1396 Fragments arranged by size: 2282 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3679 3866 1700 Size: 110 1869 187 2869 Fragments arranged by size: 2869 1869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5035 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5035 BssSI C'ACGA_G Cuts at: 3095 4107 3095 Size: 1012 4023 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5035 BstBI TT'CG_AA Cuts at: 3839 3839 Size: 5035 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5035 BstYI r'GATC_y Cuts at: 1972 2365 2732 3117 3134 4586 4672 4684 5030 Size: 393 367 385 17 1452 86 12 346 Cuts at: 5030 1972 Size: 1977 Fragments arranged by size: 1977 1452 393 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2221 513 Size: 552 939 217 3327 Fragments arranged by size: 3327 939 552 217 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4296 Fragments arranged by size: 4296 683 56 BtsI GCAGTG_nn' Cuts at: 2088 2551 3345 3365 2088 Size: 463 794 20 3758 Fragments arranged by size: 3758 794 463 20 ClaI AT'CG_AT Cuts at: 1554 2132 1554 Size: 578 4457 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5000 DraI TTT'AAA Cuts at: 2339 2339 Size: 5035 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4042 809 Size: 507 2726 1802 Fragments arranged by size: 2726 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2482 3371 5000 Size: 54 1425 50 275 18 652 889 1629 Cuts at: 5000 8 Size: 43 Fragments arranged by size: 1629 1425 889 652 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2482 1537 Size: 275 670 4090 Fragments arranged by size: 4090 670 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2965 1353 Size: 1612 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3622 3996 4142 387 Size: 1064 138 2033 374 146 1280 Fragments arranged by size: 2033 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3098 3695 4482 4750 1239 Size: 393 1466 597 787 268 1524 Fragments arranged by size: 1524 1466 787 597 393 268 EcoRI G'AATT_C Cuts at: 2344 2722 2344 Size: 378 4657 FspI TGC'GCA Cuts at: 3541 3541 Size: 5035 HaeII r_GCGC'y Cuts at: 1678 4182 1678 Size: 2504 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2251 2289 2321 3676 Size: 32 135 32 647 32 38 32 1355 Cuts at: 3676 3708 3750 3782 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1355 647 135 42 38 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2379 2625 669 Size: 988 281 441 246 3079 Fragments arranged by size: 3079 988 441 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5035 HpaI GTT'AAC Cuts at: 2625 2625 Size: 5035 KpnI G_GTAC'C Cuts at: 2360 2360 Size: 5035 MfeI C'AATT_G Cuts at: 2634 2634 Size: 5035 MluI A'CGCG_T Cuts at: 4911 4911 Size: 5035 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4149 4333 1305 Size: 2844 184 2007 Fragments arranged by size: 2844 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3213 Fragments arranged by size: 3213 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2020 2223 2993 3352 3511 518 Size: 648 854 203 770 359 159 2042 Fragments arranged by size: 2042 854 770 648 359 203 159 MspA1I CmG'CkG Cuts at: 1606 2451 3128 4276 4521 1606 Size: 845 677 1148 245 2120 Fragments arranged by size: 2120 1148 845 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3544 Fragments arranged by size: 3544 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5035 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5035 NotI GC'GGCC_GC Cuts at: 2482 2482 Size: 5035 NspI r_CATG'y Cuts at: 1132 1229 2393 3938 1132 Size: 97 1164 1545 2229 Fragments arranged by size: 2229 1545 1164 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5035 PciI A'CATG_T Cuts at: 3934 3934 Size: 5035 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5035 PmeI GTTT'AAAC Cuts at: 2339 2339 Size: 5035 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3104 3136 4617 4649 3104 Size: 32 1481 32 3490 Fragments arranged by size: 3490 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5035 PsiI TTA'TAA Cuts at: 2605 2605 Size: 5035 PstI C_TGCA'G Cuts at: 830 2387 830 Size: 1557 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5035 SalI G'TCGA_C Cuts at: 1936 2377 1936 Size: 441 4594 SbfI CC_TGCA'GG Cuts at: 2387 2387 Size: 5035 ScaI AGT'ACT Cuts at: 3283 4995 3283 Size: 1712 3323 SfcI C'TryA_G Cuts at: 826 1045 2383 3518 4199 4390 826 Size: 219 1338 1135 681 191 1471 Fragments arranged by size: 1471 1338 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5035 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5035 SmaI CCC'GGG Cuts at: 1872 2362 1872 Size: 490 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3139 4040 4302 820 Size: 197 926 1196 901 262 1553 Fragments arranged by size: 1553 1196 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2826 493 Size: 2333 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5035 SphI G_CATG'C Cuts at: 1132 2393 1132 Size: 1261 3774 SspI AAT'ATT Cuts at: 5 52 2959 5 Size: 47 2907 2081 Fragments arranged by size: 2907 2081 47 StuI AGG'CCT Cuts at: 1517 2045 1517 Size: 528 4507 StyI C'CwwG_G Cuts at: 513 1065 2004 2474 513 Size: 552 939 470 3074 Fragments arranged by size: 3074 939 552 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2014 3062 3079 3232 3417 3862 325 Size: 1255 434 1048 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1048 445 434 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2215 3281 4993 Size: 274 80 33 51 1443 238 1066 1712 Cuts at: 4993 96 Size: 138 Fragments arranged by size: 1712 1443 1066 274 238 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4995 5027 4995 Size: 32 5003 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5035 XbaI T'CTAG_A Cuts at: 2371 2371 Size: 5035 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5035 XmaI C'CCGG_G Cuts at: 1870 2360 1870 Size: 490 4545 XmnI GAAnn'nnTTC Cuts at: 3164 3164 Size: 5035 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2843 276 Size: 53 83 186 936 1309 2468 Fragments arranged by size: 2468 1309 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI