################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BcgI BciVI BglI BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FokI FseI FspI HgaI Hin1I HincII HindII HpaI HphI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 300 GTCTAC GT^[AC][GT]AC AcsI 39 GAATTT [GA]^AATT[CT] AflII 30 CTTAAG C^TTAAG AlwNI 941 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 26 AGCGCT AGC^GCT ApoI 39 GAATTT [GA]^AATT[CT] AseI 769 ATTAAT AT^TAAT AsnI 769 ATTAAT AT^TAAT BamHI 158 GGATCC G^GATCC BbvCI 938 CCTCAGC CCTCAGC(-5/-2) BclI 363 TGATCA T^GATCA BfaI 274 CTAG C^TAG BfrI 30 CTTAAG C^TTAAG BglII 579 AGATCT A^GATCT BlpI 22 GCTGAGC GC^T[ACGT]AGC Bpu1102I 22 GCTGAGC GC^T[ACGT]AGC BsgI 794 CTGCAC GTGCAG(16/14) BsmAI 574 GAGAC GTCTC(1/5) BsmFI 753 GGGAC GGGAC(10/14) BssSI 193 CTCGTG CACGAG(-5/-1) BstXI 473 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 22 GCTGAGC GC^T[ACGT]AGC CfoI 27 GCGC GCG^C DraI 16 TTTAAA TTT^AAA DraII 621 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 203 CCATGG C^C[GA][CT]GG Eco47III 26 AGCGCT AGC^GCT EcoO109I 621 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 203 CCATGG C^C[AT][AT]GG FbaI 363 TGATCA T^GATCA HaeII 26 AGCGCT [GA]GCGC^[CT] HhaI 27 GCGC GCG^C HinP1I 27 GCGC G^CGC HindIII 838 AAGCTT A^AGCTT MaeI 274 CTAG C^TAG MaeII 521 ACGT A^CGT MaeIII 227 GTGAC ^GT[ACGT]AC NcoI 203 CCATGG C^CATGG PflMI 658 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 326 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 769 ATTAAT AT^TAAT PstI 854 CTGCAG CTGCA^G SacI 152 GAGCTC GAGCT^C SapI 985 GAAGAGC GCTCTTC(1/4) SfcI 854 CTGCAG C^T[GA][CT]AG SmlI 30 CTTAAG C^T[CT][GA]AG StyI 203 CCATGG C^C[AT][AT]GG TaqI 324 TCGA T^CGA Tsp45I 227 GTGAC ^GT[GC]AC TspRI 743 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 324 TCGA T^CGA Van91I 658 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 222 GTAC GT^AC 523 GTAC AspHI 152 GAGCTC G[AT]GC[AT]^C 169 GAGCAC BanI 681 GGTGCC G^G[CT][GA]CC 928 GGTGCC BanII 6 GGGCTC G[GA]GC[CT]^C 152 GAGCTC BbsI 184 GAAGAC GAAGAC(2/6) 949 GAAGAC BcnI 180 CCGGG CC^[GC]GG 974 CCCGG BpmI 328 CTCCAG CTGGAG(16/14) 214 CTGGAG BpuAI 184 GAAGAC GAAGAC(2/6) 949 GAAGAC BsiHKAI 152 GAGCTC G[AT]GC[AT]^C 169 GAGCAC BspMI 230 ACCTGC ACCTGC(4/8) 852 ACCTGC DrdI 538 GACTCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 868 GACAACTTTGTC EcoNI 192 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 625 CCTGGAAGAGG HapII 180 CCGG C^CGG 975 CCGG HpaII 180 CCGG C^CGG 975 CCGG MspI 180 CCGG C^CGG 975 CCGG MwoI 97 GCCCAGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 891 GCCATCTGTGC NciI 180 CCGGG CC^[GC]GG 974 CCCGG PleI 326 GACTC GAGTC(4/5) 538 GACTC RsaI 222 GTAC GT^AC 523 GTAC SfaNI 919 GCATC GCATC(5/9) 943 GCATC XcmI 203 CCATGGAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 473 CCAAAATCCTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AluI AlwI AvaII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI HaeIII HinfI ItaI Ksp632I MboI MboII MflI MnlI MseI MslI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp509I XhoII