(Circular) (Six-base) MAPSORT of: pFN21AE5449.seq Check: 6464 from: 1 to: 5710 pFN21AE5449 5710 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2306 3053 1937 Size: 369 747 4594 Fragments arranged by size: 4594 747 369 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2973 3773 5157 5425 1632 Size: 1341 800 1384 268 1917 Fragments arranged by size: 1917 1384 1341 800 268 AfeI AGC'GCT Cuts at: 2033 2033 Size: 5710 AflII C'TTAA_G Cuts at: 820 1017 2035 820 Size: 197 1018 4495 Fragments arranged by size: 4495 1018 197 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 2951 3453 5025 2951 Size: 502 1572 3636 Fragments arranged by size: 3636 1572 502 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 2044 3019 3245 3397 5506 1493 Size: 551 975 226 152 2109 1697 Fragments arranged by size: 2109 1697 975 551 226 152 AseI AT'TA_AT Cuts at: 160 1032 2775 4264 160 Size: 872 1743 1489 1606 Fragments arranged by size: 1743 1606 1489 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 3035 1870 Size: 73 1092 4545 Fragments arranged by size: 4545 1092 73 BamHI G'GATC_C Cuts at: 2163 3040 3407 2163 Size: 877 367 4466 Fragments arranged by size: 4466 877 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2686 2933 3031 4486 618 Size: 325 217 627 899 247 98 1455 1842 Fragments arranged by size: 1842 1455 899 627 325 247 217 98 BanII G_rGCy'C Cuts at: 1391 2015 2161 3029 1391 Size: 624 146 868 4072 Fragments arranged by size: 4072 868 624 146 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2196 2961 928 Size: 971 297 765 3677 Fragments arranged by size: 3677 971 765 297 BbvCI CC'TCA_GC Cuts at: 2944 2944 Size: 5710 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3901 3935 1903 Size: 34 50 34 1880 34 3678 Fragments arranged by size: 3678 1880 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 2368 1548 Size: 820 4890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4321 136 Size: 107 122 71 631 605 2649 1525 Fragments arranged by size: 2649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2584 5705 2584 Size: 3121 2589 BlpI GC'TnA_GC Cuts at: 2028 3127 2028 Size: 1099 4611 Bme1580I G_kGCm'C Cuts at: 1790 3771 4927 1790 Size: 1981 1156 2573 Fragments arranged by size: 2573 1981 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 2318 4370 1239 Size: 1001 78 2052 2579 Fragments arranged by size: 2579 2052 1001 78 Bpu10I CC'TnA_GC Cuts at: 2944 2944 Size: 5710 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4373 882 Size: 617 2874 2219 Fragments arranged by size: 2874 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3517 3899 4534 275 Size: 53 83 186 936 1984 382 635 1451 Fragments arranged by size: 1984 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2316 2556 2652 1186 Size: 1130 240 96 4244 Fragments arranged by size: 4244 1130 240 96 BseYI C'CCAG_C Cuts at: 2008 2141 4913 5409 2008 Size: 133 2772 496 2309 Fragments arranged by size: 2772 2309 496 133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2784 1163 Size: 573 1048 4089 Fragments arranged by size: 4089 1048 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2161 2178 3771 3856 4927 2161 Size: 17 1593 85 1071 2944 Fragments arranged by size: 2944 1593 1071 85 17 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2161 2178 3029 3771 3856 4927 Size: 399 225 146 17 851 742 85 1071 Cuts at: 4927 1391 Size: 2174 Fragments arranged by size: 2174 1071 851 742 399 225 146 85 17 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 2866 3065 844 Size: 1400 622 199 3489 Fragments arranged by size: 3489 1400 622 199 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4205 4379 65 Size: 1183 2957 174 1396 Fragments arranged by size: 2957 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4354 4541 1700 Size: 110 2544 187 2869 Fragments arranged by size: 2869 2544 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 2198 3770 4782 2198 Size: 1572 1012 3126 Fragments arranged by size: 3126 1572 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstXI CCAn_nnnn'nTGG Cuts at: 2485 2485 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2163 2214 2584 3040 3407 3792 3809 5261 Size: 191 51 370 456 367 385 17 1452 Cuts at: 5261 5347 5359 5705 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 456 385 370 367 346 191 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2208 513 Size: 552 1143 4015 Fragments arranged by size: 4015 1143 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 2747 3226 4020 4040 2747 Size: 479 794 20 4417 Fragments arranged by size: 4417 794 479 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 2023 3014 2023 Size: 991 4719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2549 2879 4717 809 Size: 507 1233 330 1838 1802 Fragments arranged by size: 1838 1802 1233 507 330 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3157 4046 5675 Size: 54 1425 50 275 18 1327 889 1629 Cuts at: 5675 8 Size: 43 Fragments arranged by size: 1629 1425 1327 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2629 2800 2985 3640 1353 Size: 1276 171 185 655 3423 Fragments arranged by size: 3423 1276 655 185 171 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2159 2159 Size: 5710 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2318 2973 3773 4370 5157 5425 Size: 393 608 78 655 800 597 787 268 Cuts at: 5425 1239 Size: 1524 Fragments arranged by size: 1524 800 787 655 608 597 393 268 78 EcoNI CCTnn'n_nnAGG Cuts at: 2201 2634 2201 Size: 433 5277 EcoO109I rG'GnC_Cy Cuts at: 2627 2627 Size: 5710 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2035 4857 1678 Size: 357 2822 2531 Fragments arranged by size: 2822 2531 357 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2148 2180 2797 2829 2882 Size: 32 135 32 576 32 617 32 53 Cuts at: 2882 2914 4351 4383 4425 4457 1373 Size: 32 1437 32 42 32 2626 Fragments arranged by size: 2626 1437 617 576 135 53 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 2843 748 Size: 2095 3615 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2578 4824 5008 1305 Size: 1273 2246 184 2007 Fragments arranged by size: 2246 2007 1273 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2702 2708 2714 3668 4027 4186 518 Size: 648 1536 6 6 954 359 159 2042 Fragments arranged by size: 2042 1536 954 648 359 159 6 6 MspA1I CmG'CkG Cuts at: 1606 3126 3803 4951 5196 1606 Size: 1520 677 1148 245 2120 Fragments arranged by size: 2120 1520 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2208 513 Size: 552 1143 4015 Fragments arranged by size: 4015 1143 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 3068 4613 1132 Size: 97 1839 1545 2229 Fragments arranged by size: 2229 1839 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 2669 1108 Size: 1561 4149 PfoI T'CCnGG_A Cuts at: 2184 2217 2815 2978 2184 Size: 33 598 163 4916 Fragments arranged by size: 4916 598 163 33 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2335 1613 Size: 722 4988 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PstI C_TGCA'G Cuts at: 830 2863 3062 830 Size: 2033 199 3478 Fragments arranged by size: 3478 2033 199 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 SacI G_AGCT'C Cuts at: 2161 2161 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SapI GCTCTTCn'nnn_ Cuts at: 2985 2985 Size: 5710 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2859 3058 4193 4874 5065 826 Size: 219 1814 199 1135 681 191 1471 Fragments arranged by size: 1814 1471 1135 681 219 199 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2035 3814 4715 4977 820 Size: 197 926 92 1779 901 262 1553 Fragments arranged by size: 1779 1553 926 901 262 197 92 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2208 3149 513 Size: 552 1143 941 3074 Fragments arranged by size: 3074 1143 941 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2491 3737 3754 3907 4092 4537 325 Size: 1255 911 1246 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1246 911 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 3956 5668 Size: 274 80 33 51 1443 249 1730 1712 Cuts at: 5668 96 Size: 138 Fragments arranged by size: 1730 1712 1443 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2466 2498 5670 5702 2466 Size: 32 3172 32 2474 Fragments arranged by size: 3172 2474 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2215 2485 2215 Size: 270 5440 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI