(Linear) (Six-base) MAPSORT of: KIEE5449.seq Check: 9164 from: 1 to: 1005 KIEE5449.orf With 182 enzymes: SgfI * January 29, 2014 12:01 .. AccI GT'mk_AC Cuts at: 0 301 1005 Size: 301 704 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 968 1005 Size: 968 37 AfeI AGC'GCT Cuts at: 0 28 1005 Size: 28 977 AflII C'TTAA_G Cuts at: 0 30 1005 Size: 30 975 AlwNI CAG_nnn'CTG Cuts at: 0 946 1005 Size: 946 59 ApoI r'AATT_y Cuts at: 0 39 1005 Size: 39 966 AseI AT'TA_AT Cuts at: 0 770 1005 Size: 770 235 BamHI G'GATC_C Cuts at: 0 158 1005 Size: 158 847 BanI G'GyrC_C Cuts at: 0 681 928 1005 Size: 681 247 77 Fragments arranged by size: 681 247 77 BanII G_rGCy'C Cuts at: 0 10 156 1005 Size: 10 146 849 Fragments arranged by size: 849 146 10 BbsI GAAGACnn'nnnn_ Cuts at: 0 191 956 1005 Size: 191 765 49 Fragments arranged by size: 765 191 49 BbvCI CC'TCA_GC Cuts at: 0 939 1005 Size: 939 66 BclI T'GATC_A Cuts at: 0 363 1005 Size: 363 642 BglII A'GATC_T Cuts at: 0 579 1005 Size: 579 426 BlpI GC'TnA_GC Cuts at: 0 23 1005 Size: 23 982 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 313 1005 Size: 235 78 692 Fragments arranged by size: 692 235 78 Bpu10I CC'TnA_GC Cuts at: 0 939 1005 Size: 939 66 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 311 551 647 1005 Size: 311 240 96 358 Fragments arranged by size: 358 311 240 96 BseYI C'CCAG_C Cuts at: 0 3 136 1005 Size: 3 133 869 Fragments arranged by size: 869 133 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 779 1005 Size: 779 226 BsiHKAI G_wGCw'C Cuts at: 0 156 173 1005 Size: 156 17 832 Fragments arranged by size: 832 156 17 Bsp1286I G_dGCh'C Cuts at: 0 10 156 173 1005 Size: 10 146 17 832 Fragments arranged by size: 832 146 17 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 239 861 1005 Size: 239 622 144 Fragments arranged by size: 622 239 144 BssSI C'ACGA_G Cuts at: 0 193 1005 Size: 193 812 BstXI CCAn_nnnn'nTGG Cuts at: 0 480 1005 Size: 480 525 BstYI r'GATC_y Cuts at: 0 158 209 579 1005 Size: 158 51 370 426 Fragments arranged by size: 426 370 158 51 BtgI C'CryG_G Cuts at: 0 203 1005 Size: 203 802 BtsI GCAGTG_nn' Cuts at: 0 742 1005 Size: 742 263 DraI TTT'AAA Cuts at: 0 18 1005 Size: 18 987 DrdI GACnn_nn'nnGTC Cuts at: 0 544 874 1005 Size: 544 330 131 Fragments arranged by size: 544 330 131 EarI CTCTTCn'nnn_ Cuts at: 0 624 795 980 1005 Size: 624 171 185 25 Fragments arranged by size: 624 185 171 25 EcoICRI GAG'CTC Cuts at: 0 154 1005 Size: 154 851 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 313 968 1005 Size: 235 78 655 37 Fragments arranged by size: 655 235 78 37 EcoNI CCTnn'n_nnAGG Cuts at: 0 196 629 1005 Size: 196 433 376 Fragments arranged by size: 433 376 196 EcoO109I rG'GnC_Cy Cuts at: 0 622 1005 Size: 622 383 HaeII r_GCGC'y Cuts at: 0 30 1005 Size: 30 975 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 143 175 792 824 877 909 1005 Size: 143 32 617 32 53 32 96 Fragments arranged by size: 617 143 96 53 32 32 32 HindIII A'AGCT_T Cuts at: 0 838 1005 Size: 838 167 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 573 1005 Size: 573 432 MslI CAynn'nnrTG Cuts at: 0 697 703 709 1005 Size: 697 6 6 296 Fragments arranged by size: 697 296 6 6 NcoI C'CATG_G Cuts at: 0 203 1005 Size: 203 802 PflMI CCAn_nnn'nTGG Cuts at: 0 664 1005 Size: 664 341 PfoI T'CCnGG_A Cuts at: 0 179 212 810 973 1005 Size: 179 33 598 163 32 Fragments arranged by size: 598 179 163 33 32 PshAI GACnn'nnGTC Cuts at: 0 330 1005 Size: 330 675 PstI C_TGCA'G Cuts at: 0 858 1005 Size: 858 147 SacI G_AGCT'C Cuts at: 0 156 1005 Size: 156 849 SapI GCTCTTCn'nnn_ Cuts at: 0 980 1005 Size: 980 25 SfcI C'TryA_G Cuts at: 0 854 1005 Size: 854 151 SmlI C'TyrA_G Cuts at: 0 30 1005 Size: 30 975 StyI C'CwwG_G Cuts at: 0 203 1005 Size: 203 802 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 486 1005 Size: 486 519 TatI w'GTAC_w Cuts at: 0 221 1005 Size: 221 784 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 461 493 1005 Size: 461 32 512 Fragments arranged by size: 512 461 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 210 480 1005 Size: 210 270 525 Fragments arranged by size: 525 270 210 Enzymes that do cut: AccI AcuI AfeI AflII AlwNI ApoI AseI BamHI BanI BanII BbsI BbvCI BclI BglII BlpI BpmI Bpu10I BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BssSI BstXI BstYI BtgI BtsI DraI DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MmeI MslI NcoI PflMI PfoI PshAI PstI SacI SapI SfcI SmlI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BfrBI BglI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EaeI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI