Restriction map for
pFN21AE2560
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BanII BbsI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI Bsp1286I Bsp1407I
BspDI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I EcoT22I
FauI FbaI FseI FspI HgaI
HincII HindII HpaI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MscI MslI MspA1I MunI MvaI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfaNI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccI 598 GTAGAC GT^[AC][GT]AC
AccII 53 CGCG CG^CG
AccIII 60 TCCGGA T^CCGGA
AciI 52 CCGC CCGC(-3/-1)
AclI 263 AACGTT AA^CGTT
AcsI 276 AAATTT [GA]^AATT[CT]
AcyI 48 GGCGCC G[GA]^CG[CT]C
ApoI 276 AAATTT [GA]^AATT[CT]
BanI 48 GGCGCC G^G[CT][GA]CC
BbeI 48 GGCGCC GGCGC^C
BbrPI 352 CACGTG CAC^GTG
BbvI 153 GCAGC GCAGC(8/12)
BglII 174 AGATCT A^GATCT
BsaAI 352 CACGTG [CT]AC^GT[GA]
BsaHI 48 GGCGCC G[GA]^CG[CT]C
BsaJI 69 CCCGGG C^C[ACGT][ACGT]GG
BsaWI 60 TCCGGA [AT]^CCGG[AT]
BseAI 60 TCCGGA T^CCGGA
BsmI 156 GCATTC GAATGC(1/-1)
BspEI 60 TCCGGA T^CCGGA
BspLU11I 192 ACATGT A^CATGT
BsrI 346 ACTGG ACTGG(1/-1)
BstUI 53 CGCG CG^CG
BstYI 174 AGATCT [GA]^GATC[CT]
Cac8I 45 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 49 GCGC GCG^C
DdeI 117 CTCAG C^T[ACGT]AG
DpnII 175 GATC ^GATC
EarI 574 GAAGAG CTCTTC(1/4)
EcoRV 511 GATATC GAT^ATC
HaeII 48 GGCGCC [GA]GCGC^[CT]
HhaI 49 GCGC GCG^C
Hin1I 48 GGCGCC G[GA]^CG[CT]C
HinP1I 49 GCGC G^CGC
HindIII 100 AAGCTT A^AGCTT
HphI 361 GGTGA GGTGA(8/7)
KasI 48 GGCGCC G^GCGCC
Ksp632I 574 GAAGAG CTCTTC(1/4)
MboI 175 GATC ^GATC
MflI 174 AGATCT [GA]^GATC[CT]
MroI 60 TCCGGA T^CCGGA
MvnI 53 CGCG CG^CG
MwoI 45 GCTGGCGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 48 GGCGCC GG^CGCC
NdeII 175 GATC ^GATC
NlaIII 193 CATG CATG^
NspI 192 ACATGT [GA]CATG^[CT]
PaeR7I 94 CTCGAG C^TCGAG
PmaCI 352 CACGTG CAC^GTG
PmlI 352 CACGTG CAC^GTG
Psp1406I 263 AACGTT AA^CGTT
Sau3AI 175 GATC ^GATC
ScaI 256 AGTACT AGT^ACT
SfcI 178 CTACAG C^T[GA][CT]AG
SfoI 48 GGCGCC GGC^GCC
SmaI 69 CCCGGG CCC^GGG
SmlI 94 CTCGAG C^T[CT][GA]AG
TaqI 95 TCGA T^CGA
TseI 153 GCAGC G^C[AT]GC
TthHB8I 95 TCGA T^CGA
XhoI 94 CTCGAG C^TCGAG
XhoII 174 AGATCT [GA]^GATC[CT]
XmaCI 69 CCCGGG C^CCGGG
XmaI 69 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 257 GTAC GT^AC
318 GTAC
AflIII 192 ACATGT A^C[GA][CT]GT
351 ACACGT
AluI 101 AGCT AG^CT
219 AGCT
AvaI 69 CCCGGG C^[CT]CG[GA]G
94 CTCGAG
BcnI 69 CCCGG CC^[GC]GG
70 CCGGG
BfaI 374 CTAG C^TAG
382 CTAG
BsiYI 70 CCGGGCTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
610 CCACCTGAAGG
BslI 70 CCGGGCTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
610 CCACCTGAAGG
BsoBI 69 CCCGGG C^[CT]CG[GA]G
94 CTCGAG
BssKI 69 CCCGG ^CC[ACGT]GG
70 CCGGG
BstF5I 63 GGATG GGATG(2/0)
297 GGATG
Cfr13I 79 GGCCC G^G[ACGT]CC
431 GGGCC
DraI 235 TTTAAA TTT^AAA
558 TTTAAA
Fnu4HI 51 GCCGC GC^[ACGT]GC
153 GCAGC
FokI 63 GGATG GGATG(9/13)
297 GGATG
HaeIII 79 GGCC GG^CC
432 GGCC
HapII 61 CCGG C^CGG
70 CCGG
HpaII 61 CCGG C^CGG
70 CCGG
ItaI 51 GCCGC GC^[ACGT]GC
153 GCAGC
MaeI 374 CTAG C^TAG
382 CTAG
MnlI 58 CCTC CCTC(7/6)
3 GAGG
MspI 61 CCGG C^CGG
70 CCGG
NciI 69 CCCGG CC^[GC]GG
70 CCGGG
PleI 92 GACTC GAGTC(4/5)
370 GACTC
RsaI 257 GTAC GT^AC
318 GTAC
Sau96I 79 GGCCC G^G[ACGT]CC
431 GGGCC
ScrFI 69 CCCGG CC^[ACGT]GG
70 CCGGG
TfiI 106 GATTC G^A[AT]TC
416 GAATC
Tsp509I 239 AATT ^AATT
277 AATT
TspRI 321 CACTG CA[GC]TG[ACGT][ACGT]^
345 CACTG
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
BsmAI HinfI MaeII MboII MseI
NlaIV Tru9I