################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BalI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BfrI BglI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI ItaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI MwoI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI Tru9I TseI Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AciI 163 CCGC CCGC(-3/-1) AlwNI 347 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 319 GGGCCC GGGCC^C AspHI 280 GAGCTC G[AT]GC[AT]^C AvrII 295 CCTAGG C^CTAGG BamHI 24 GGATCC G^GATCC BglII 376 AGATCT A^GATCT BlnI 295 CCTAGG C^CTAGG BpmI 389 CTCCAG CTGGAG(16/14) BsaWI 66 TCCGGT [AT]^CCGG[AT] BseRI 31 CTCCTC GAGGAG(10/8) BsgI 151 GTGCAG GTGCAG(16/14) BsiHKAI 280 GAGCTC G[AT]GC[AT]^C BsmAI 229 GAGAC GTCTC(1/5) BsmFI 343 GTCCC GGGAC(10/14) BsrBI 163 CCGCTC CCGCTC(-3/-3) BstXI 139 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 113 GCGC GCG^C EarI 277 GAAGAG CTCTTC(1/4) HhaI 113 GCGC GCG^C HinP1I 113 GCGC G^CGC Ksp632I 277 GAAGAG CTCTTC(1/4) MaeIII 103 GTTAC ^GT[ACGT]AC NcoI 417 CCATGG C^CATGG NlaIII 418 CATG CATG^ PflMI 346 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 280 GAGCTC GAGCT^C SapI 277 GAAGAGC GCTCTTC(1/4) SspI 169 AATATT AAT^ATT StuI 286 AGGCCT AGG^CCT TspRI 99 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 346 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AfaI 70 GTAC GT^AC 426 GTAC AvaII 120 GGACC G^G[AT]CC 342 GGTCC BanI 218 GGCACC G^G[CT][GA]CC 413 GGTGCC BanII 280 GAGCTC G[GA]GC[CT]^C 319 GGGCCC BcnI 8 CCCGG CC^[GC]GG 36 CCGGG BmyI 280 GAGCTC G[AGT]GC[ACT]^C 319 GGGCCC Bsp1286I 280 GAGCTC G[AGT]GC[ACT]^C 319 GGGCCC DsaI 116 CCGTGG C^C[GA][CT]GG 417 CCATGG HinfI 63 GATTC G^A[ACGT]TC 190 GATTC MboII 277 GAAGA GAAGA(8/7) 374 GAAGA NciI 8 CCCGG CC^[GC]GG 36 CCGGG RsaI 70 GTAC GT^AC 426 GTAC TfiI 63 GATTC G^A[AT]TC 190 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AluI AlwI BfaI BsaJI BsiYI BslI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I HaeIII HapII HpaII MaeI MboI MflI MnlI MspI MvaI NdeII NlaIV Sau3AI Sau96I ScrFI StyI XhoII