(Circular) (Six-base) MAPSORT of: pFN21AE6579.seq Check: 8834 from: 1 to: 5137 pFN21AE6579 5137 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2947 278 Size: 53 83 186 936 1411 2468 Fragments arranged by size: 2468 1411 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2480 1937 Size: 543 4594 Acc65I G'GTAC_C Cuts at: 2458 2458 Size: 5137 AclI AA'CG_TT Cuts at: 1563 3264 3637 1563 Size: 1701 373 3063 Fragments arranged by size: 3063 1701 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3200 4584 4852 1632 Size: 1568 1384 268 1917 Fragments arranged by size: 1917 1568 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4940 AflIII A'CryG_T Cuts at: 1203 4036 5013 1203 Size: 2833 977 1327 Fragments arranged by size: 2833 1327 977 AgeI A'CCGG_T Cuts at: 3968 3968 Size: 5137 AhdI GACnn_n'nnGTC Cuts at: 3866 3866 Size: 5137 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5137 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5137 AlwNI CAG_nnn'CTG Cuts at: 2357 2880 4452 2357 Size: 523 1572 3042 Fragments arranged by size: 3042 1572 523 ApaI G_GGCC'C Cuts at: 2328 2328 Size: 5137 ApaLI G'TGCA_C Cuts at: 3194 4350 3194 Size: 1156 3981 ApoI r'AATT_y Cuts at: 1493 2446 2672 2824 4933 1493 Size: 953 226 152 2109 1697 Fragments arranged by size: 2109 1697 953 226 152 AseI AT'TA_AT Cuts at: 160 1032 3691 160 Size: 872 2659 1606 Fragments arranged by size: 2659 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5137 AvaI C'yCGr_G Cuts at: 1870 1943 2462 1870 Size: 73 519 4545 Fragments arranged by size: 4545 519 73 AvrII C'CTAG_G Cuts at: 2300 2300 Size: 5137 BamHI G'GATC_C Cuts at: 2029 2467 2834 2029 Size: 438 367 4332 Fragments arranged by size: 4332 438 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2223 2418 2458 3913 618 Size: 325 217 627 436 195 40 1455 1842 Fragments arranged by size: 1842 1455 627 436 325 217 195 40 BanII G_rGCy'C Cuts at: 1391 2289 2328 2456 1391 Size: 898 39 128 4072 Fragments arranged by size: 4072 898 128 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3328 3362 1903 Size: 34 1391 34 3678 Fragments arranged by size: 3678 1391 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3029 4239 3029 Size: 1210 3927 BclI T'GATC_A Cuts at: 1548 1548 Size: 5137 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3748 136 Size: 107 122 71 631 605 2076 1525 Fragments arranged by size: 2076 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2381 5132 2381 Size: 2751 2386 BlpI GC'TnA_GC Cuts at: 2554 2554 Size: 5137 Bme1580I G_kGCm'C Cuts at: 1790 2328 3198 4354 1790 Size: 538 870 1156 2573 Fragments arranged by size: 2573 1156 870 538 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5137 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2868 3826 442 Size: 416 53 83 399 24 1451 958 1753 Fragments arranged by size: 1753 1451 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5137 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2379 3797 1239 Size: 1140 1418 2579 Fragments arranged by size: 2579 1418 1140 Bpu10I CC'TnA_GC Cuts at: 2288 2288 Size: 5137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3262 4127 4425 3262 Size: 865 298 3974 Fragments arranged by size: 3974 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3800 882 Size: 617 2301 2219 Fragments arranged by size: 2301 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2928 493 Size: 711 1724 2702 Fragments arranged by size: 2702 1724 711 BsaBI GATnn'nnATC Cuts at: 5131 5131 Size: 5137 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2944 3326 3961 275 Size: 53 83 186 936 1411 382 635 1451 Fragments arranged by size: 1451 1411 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2071 3570 3968 4242 4389 4800 2071 Size: 1499 398 274 147 411 2408 Fragments arranged by size: 2408 1499 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4912 Fragments arranged by size: 4912 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 1186 Size: 841 4296 BseYI C'CCAG_C Cuts at: 2350 4340 4836 2350 Size: 1990 496 2651 Fragments arranged by size: 2651 1990 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 1163 Size: 573 441 4123 Fragments arranged by size: 4123 573 441 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2587 3348 4376 1540 Size: 275 186 586 761 1028 2301 Fragments arranged by size: 2301 1028 761 586 275 186 BsiHKAI G_wGCw'C Cuts at: 2289 3198 3283 4354 2289 Size: 909 85 1071 3072 Fragments arranged by size: 3072 1071 909 85 BsmI GAATG_Cn' Cuts at: 2648 2741 2648 Size: 93 5044 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2289 2328 2456 3198 3283 4354 1391 Size: 399 499 39 128 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 499 399 128 85 39 BspEI T'CCGG_A Cuts at: 4800 4800 Size: 5137 BspHI T'CATG_A Cuts at: 3026 4756 3026 Size: 1730 3407 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2492 844 Size: 1648 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2170 3024 1394 Size: 429 347 854 3507 Fragments arranged by size: 3507 854 429 347 BsrDI GCAATG_nn' Cuts at: 65 1248 3632 3806 65 Size: 1183 2384 174 1396 Fragments arranged by size: 2384 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3781 3968 1700 Size: 110 1971 187 2869 Fragments arranged by size: 2869 1971 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5137 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5137 BssSI C'ACGA_G Cuts at: 3197 4209 3197 Size: 1012 4125 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5137 BstBI TT'CG_AA Cuts at: 3941 3941 Size: 5137 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5137 BstXI CCAn_nnnn'nTGG Cuts at: 2151 2151 Size: 5137 BstYI r'GATC_y Cuts at: 1972 2029 2140 2381 2467 2834 3219 3236 4688 Size: 57 111 241 86 367 385 17 1452 Cuts at: 4688 4774 4786 5132 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 241 111 86 86 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2121 2422 513 Size: 552 1056 301 3228 Fragments arranged by size: 3228 1056 552 301 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4398 Fragments arranged by size: 4398 683 56 BtsI GCAGTG_nn' Cuts at: 2653 3447 3467 2653 Size: 794 20 4323 Fragments arranged by size: 4323 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5137 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5102 DraI TTT'AAA Cuts at: 2441 2441 Size: 5137 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4144 809 Size: 507 2828 1802 Fragments arranged by size: 2828 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2584 3473 5102 Size: 54 1425 50 275 18 754 889 1629 Cuts at: 5102 8 Size: 43 Fragments arranged by size: 1629 1425 889 754 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2584 1537 Size: 275 772 4090 Fragments arranged by size: 4090 772 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2277 3067 1353 Size: 924 790 3423 Fragments arranged by size: 3423 924 790 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3724 4098 4244 387 Size: 1064 138 2135 374 146 1280 Fragments arranged by size: 2135 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2287 2287 Size: 5137 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 3200 3797 4584 4852 1239 Size: 393 747 821 597 787 268 1524 Fragments arranged by size: 1524 821 787 747 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2204 2318 2325 2204 Size: 114 7 5016 Fragments arranged by size: 5016 114 7 EcoRI G'AATT_C Cuts at: 2446 2824 2446 Size: 378 4759 FspI TGC'GCA Cuts at: 3643 3643 Size: 5137 HaeII r_GCGC'y Cuts at: 1678 4284 1678 Size: 2606 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3778 3810 3852 3884 1373 Size: 32 135 32 2206 32 42 32 2626 Fragments arranged by size: 2626 2206 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2481 2727 669 Size: 988 281 543 246 3079 Fragments arranged by size: 3079 988 543 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5137 HpaI GTT'AAC Cuts at: 2727 2727 Size: 5137 KpnI G_GTAC'C Cuts at: 2462 2462 Size: 5137 MfeI C'AATT_G Cuts at: 2736 2736 Size: 5137 MluI A'CGCG_T Cuts at: 5013 5013 Size: 5137 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4251 4435 1305 Size: 2946 184 2007 Fragments arranged by size: 2946 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3315 Fragments arranged by size: 3315 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3095 3454 3613 518 Size: 648 1929 359 159 2042 Fragments arranged by size: 2042 1929 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2553 3230 4378 4623 1606 Size: 947 677 1148 245 2120 Fragments arranged by size: 2120 1148 947 677 245 NcoI C'CATG_G Cuts at: 513 1065 2422 513 Size: 552 1357 3228 Fragments arranged by size: 3228 1357 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5137 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5137 NotI GC'GGCC_GC Cuts at: 2584 2584 Size: 5137 NspI r_CATG'y Cuts at: 1132 1229 2495 4040 1132 Size: 97 1266 1545 2229 Fragments arranged by size: 2229 1545 1266 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5137 PciI A'CATG_T Cuts at: 4036 4036 Size: 5137 PflMI CCAn_nnn'nTGG Cuts at: 1108 2357 1108 Size: 1249 3888 PfoI T'CCnGG_A Cuts at: 2356 2356 Size: 5137 PmeI GTTT'AAAC Cuts at: 2441 2441 Size: 5137 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3206 3238 4719 4751 3206 Size: 32 1481 32 3592 Fragments arranged by size: 3592 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5137 PsiI TTA'TAA Cuts at: 2707 2707 Size: 5137 PspOMI G'GGCC_C Cuts at: 2324 2324 Size: 5137 PstI C_TGCA'G Cuts at: 830 2489 830 Size: 1659 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5137 SacI G_AGCT'C Cuts at: 2289 2289 Size: 5137 SalI G'TCGA_C Cuts at: 1936 2479 1936 Size: 543 4594 SapI GCTCTTCn'nnn_ Cuts at: 2277 2277 Size: 5137 SbfI CC_TGCA'GG Cuts at: 2489 2489 Size: 5137 ScaI AGT'ACT Cuts at: 3385 5097 3385 Size: 1712 3425 SexAI A'CCwGG_T Cuts at: 2433 2433 Size: 5137 SfcI C'TryA_G Cuts at: 826 1045 2485 3620 4301 4492 826 Size: 219 1440 1135 681 191 1471 Fragments arranged by size: 1471 1440 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5137 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5137 SmaI CCC'GGG Cuts at: 1872 2464 1872 Size: 592 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3241 4142 4404 820 Size: 197 926 1298 901 262 1553 Fragments arranged by size: 1553 1298 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2928 493 Size: 2435 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5137 SphI G_CATG'C Cuts at: 1132 2495 1132 Size: 1363 3774 SspI AAT'ATT Cuts at: 5 52 2176 3061 5 Size: 47 2124 885 2081 Fragments arranged by size: 2124 2081 885 47 StuI AGG'CCT Cuts at: 1517 2293 1517 Size: 776 4361 StyI C'CwwG_G Cuts at: 513 1065 2144 2277 2300 2422 2576 513 Size: 552 1079 133 23 122 154 3074 Fragments arranged by size: 3074 1079 552 154 133 122 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2143 3164 3181 3334 3519 3964 325 Size: 1255 563 1021 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1021 563 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3383 5095 96 Size: 274 80 33 51 1443 1406 1712 138 Fragments arranged by size: 1712 1443 1406 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2014 2046 5097 5129 2014 Size: 32 3051 32 2022 Fragments arranged by size: 3051 2022 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5137 XbaI T'CTAG_A Cuts at: 2473 2473 Size: 5137 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5137 XmaI C'CCGG_G Cuts at: 1870 2462 1870 Size: 592 4545 XmnI GAAnn'nnTTC Cuts at: 3266 3266 Size: 5137 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2945 276 Size: 53 83 186 936 1411 2468 Fragments arranged by size: 2468 1411 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI