Restriction map for
pFN21AE5769
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BciVI BfrI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BseRI BsiEI BsiWI Bsp1407I
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 582 GGCGTC G[GA]^CG[CT]C
AflIII 950 ACGTGT A^C[GA][CT]GT
Alw44I 374 GTGCAC G^TGCAC
AlwI 410 GGATC GGATC(4/5)
ApaI 401 GGGCCC GGGCC^C
ApaLI 374 GTGCAC G^TGCAC
BbsI 700 GTCTTC GAAGAC(2/6)
BbvCI 537 CCTCAGC CCTCAGC(-5/-2)
BcgI 230 GCACCAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1430 TGATCA T^GATCA
BglI 455 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1116 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1116 GCTCAGC GC^T[ACGT]AGC
BpuAI 700 GTCTTC GAAGAC(2/6)
BsaHI 582 GGCGTC G[GA]^CG[CT]C
BsmI 1038 GAATGC GAATGC(1/-1)
BspDI 1093 ATCGAT AT^CGAT
BspHI 1511 TCATGA T^CATGA
BsrBI 1544 GAGCGG CCGCTC(-3/-3)
BsrFI 339 GCCGGC [GA]^CCGG[CT]
BstAPI 859 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1116 GCTCAGC GC^T[ACGT]AGC
Cfr10I 339 GCCGGC [GA]^CCGG[CT]
ClaI 1093 ATCGAT AT^CGAT
FbaI 1430 TGATCA T^GATCA
Hin1I 582 GGCGTC G[GA]^CG[CT]C
MaeIII 295 GTGAC ^GT[ACGT]AC
MslI 641 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 339 GCCGGC GCC^GGC
NgoMIV 339 GCCGGC G^CCGGC
PflMI 723 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1511 TCATGA T^CATGA
SapI 600 GCTCTTC GCTCTTC(1/4)
SbfI 856 CCTGCAGG CCTGCA^GG
SmlI 976 CTCAAG C^T[CT][GA]AG
Sse8387I 856 CCTGCAGG CCTGCA^GG
Tsp45I 295 GTGAC ^GT[GC]AC
Van91I 723 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 216 CGCG CG^CG
501 CGCG
BfaI 31 CTAG C^TAG
1456 CTAG
BsaI 673 GAGACC GGTCTC(1/5)
1063 GAGACC
BspMI 1052 ACCTGC ACCTGC(4/8)
1332 ACCTGC
BsrDI 741 CATTGC GCAATG(2/0)
1551 CATTGC
BstUI 216 CGCG CG^CG
501 CGCG
DrdI 145 GACTCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1155 GACAGGCACGTC
EarI 601 CTCTTC CTCTTC(1/4)
1737 GAAGAG
FauI 1466 GCGGG CCCGC(4/6)
1475 GCGGG
HaeII 54 GGCGCT [GA]GCGC^[CT]
1195 AGCGCC
Ksp632I 601 CTCTTC CTCTTC(1/4)
1737 GAAGAG
MaeI 31 CTAG C^TAG
1456 CTAG
MspA1I 481 CAGCTG C[AC]G^C[GT]G
760 CAGCTG
MvnI 216 CGCG CG^CG
501 CGCG
NspI 519 GCATGT [GA]CATG^[CT]
1569 GCATGT
PvuII 481 CAGCTG CAG^CTG
760 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AfaI AluI AlwNI AocI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeII MboI MboII MluNI
MnlI MscI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TseI
TspRI TthHB8I XcmI XmaCI XmaI