(Circular) (Six-base) MAPSORT of: pFN21AE5769.seq Check: 6943 from: 1 to: 6466 pFN21AE5769 6466 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3346 3346 Size: 6466 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4276 278 Size: 53 83 186 936 2740 2468 Fragments arranged by size: 2740 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3809 1937 Size: 1872 4594 Acc65I G'GTAC_C Cuts at: 3787 3787 Size: 6466 AclI AA'CG_TT Cuts at: 1563 4593 4966 1563 Size: 3030 373 3063 Fragments arranged by size: 3063 3030 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2961 3598 4529 5913 6181 1632 Size: 1329 637 931 1384 268 1917 Fragments arranged by size: 1917 1384 1329 931 637 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6269 AflIII A'CryG_T Cuts at: 1203 2955 5365 6342 1203 Size: 1752 2410 977 1327 Fragments arranged by size: 2410 1752 1327 977 AgeI A'CCGG_T Cuts at: 5297 5297 Size: 6466 AhdI GACnn_n'nnGTC Cuts at: 5195 5195 Size: 6466 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6466 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6466 AlwNI CAG_nnn'CTG Cuts at: 2389 2734 2782 4209 5781 2389 Size: 345 48 1427 1572 3074 Fragments arranged by size: 3074 1572 1427 345 48 ApaI G_GGCC'C Cuts at: 2410 2410 Size: 6466 ApaLI G'TGCA_C Cuts at: 2379 4523 5679 2379 Size: 2144 1156 3166 Fragments arranged by size: 3166 2144 1156 ApoI r'AATT_y Cuts at: 1493 3775 4001 4153 6262 1493 Size: 2282 226 152 2109 1697 Fragments arranged by size: 2282 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5020 160 Size: 872 3988 1606 Fragments arranged by size: 3988 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2331 2359 2426 2798 3051 3312 Size: 73 67 321 28 67 372 253 261 Cuts at: 3312 3791 1870 Size: 479 4545 Fragments arranged by size: 4545 479 372 321 261 253 73 67 67 28 BamHI G'GATC_C Cuts at: 3796 4163 3796 Size: 367 6099 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2341 2422 2821 2870 3323 Size: 325 217 627 554 81 399 49 453 Cuts at: 3323 3421 3787 5242 618 Size: 98 366 1455 1842 Fragments arranged by size: 1842 1455 627 554 453 399 366 325 217 98 81 49 BanII G_rGCy'C Cuts at: 1391 2201 2410 2436 3288 3370 3489 3609 3785 Size: 810 209 26 852 82 119 120 176 Cuts at: 3785 1391 Size: 4072 Fragments arranged by size: 4072 852 810 209 176 120 119 82 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2698 928 Size: 971 799 4696 Fragments arranged by size: 4696 971 799 BbvCI CC'TCA_GC Cuts at: 2543 2543 Size: 6466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2224 2258 4657 4691 1903 Size: 34 287 34 2399 34 3678 Fragments arranged by size: 3678 2399 287 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4358 5568 4358 Size: 1210 5256 BclI T'GATC_A Cuts at: 1548 3435 1548 Size: 1887 4579 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2466 5077 136 Size: 107 122 71 631 605 794 2611 1525 Fragments arranged by size: 2611 1525 794 631 605 122 107 71 BglII A'GATC_T Cuts at: 6461 6461 Size: 6466 BlpI GC'TnA_GC Cuts at: 3122 3883 3122 Size: 761 5705 Bme1580I G_kGCm'C Cuts at: 1790 2383 2402 2410 2427 2826 3426 4527 5683 Size: 593 19 8 17 399 600 1101 1156 Cuts at: 5683 1790 Size: 2573 Fragments arranged by size: 2573 1156 1101 600 593 399 19 17 8 BmgBI CAC'GTC Cuts at: 1318 3168 1318 Size: 1850 4616 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2404 2405 3383 Size: 416 53 83 399 24 987 1 978 Cuts at: 3383 4197 5155 442 Size: 814 958 1753 Fragments arranged by size: 1753 987 978 958 814 416 399 83 53 24 1 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6466 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2201 2201 Size: 6466 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2696 2837 3023 5126 1239 Size: 932 525 141 186 2103 2579 Fragments arranged by size: 2579 2103 932 525 186 141 Bpu10I CC'TnA_GC Cuts at: 2543 2543 Size: 6466 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2966 4591 5456 5754 2966 Size: 1625 865 298 3678 Fragments arranged by size: 3678 1625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2672 3062 5129 882 Size: 617 1173 390 2067 2219 Fragments arranged by size: 2219 2067 1173 617 390 BsaAI yAC'GTr Cuts at: 493 1204 4257 493 Size: 711 3053 2702 Fragments arranged by size: 3053 2702 711 BsaBI GATnn'nnATC Cuts at: 6460 6460 Size: 6466 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2588 4273 4655 5290 Size: 53 83 186 936 1055 1685 382 635 Cuts at: 5290 275 Size: 1451 Fragments arranged by size: 1685 1451 1055 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4899 5297 5571 5718 6129 4899 Size: 398 274 147 411 5236 Fragments arranged by size: 5236 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3231 3261 3342 3372 1178 Size: 30 165 30 1828 30 81 30 4272 Fragments arranged by size: 4272 1828 165 81 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6466 BseYI C'CCAG_C Cuts at: 2044 2722 3010 3061 3078 3184 5669 6165 2044 Size: 678 288 51 17 106 2485 496 2345 Fragments arranged by size: 2485 2345 678 496 288 106 51 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2218 2540 2643 3647 1163 Size: 573 482 322 103 1004 3982 Fragments arranged by size: 3982 1004 573 482 322 103 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3916 4677 5705 1540 Size: 275 186 1915 761 1028 2301 Fragments arranged by size: 2301 1915 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2383 2436 3288 3370 3489 4527 4612 5683 2383 Size: 53 852 82 119 1038 85 1071 3166 Fragments arranged by size: 3166 1071 1038 852 119 85 82 53 BsmI GAATG_Cn' Cuts at: 3049 3977 4070 3049 Size: 928 93 5445 Fragments arranged by size: 5445 928 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2330 2969 3733 2330 Size: 639 764 5063 Fragments arranged by size: 5063 764 639 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 2383 2402 2410 2427 2436 2826 Size: 399 411 182 19 8 17 9 390 Cuts at: 2826 3288 3370 3426 3489 3609 3785 4527 4612 Size: 462 82 56 63 120 176 742 85 Cuts at: 4612 5683 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 462 411 399 390 182 176 120 85 82 63 56 19 17 9 8 BspEI T'CCGG_A Cuts at: 6129 6129 Size: 6466 BspHI T'CATG_A Cuts at: 3516 4355 6085 3516 Size: 839 1730 3897 Fragments arranged by size: 3897 1730 839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3066 3346 3821 844 Size: 2222 280 475 3489 Fragments arranged by size: 3489 2222 475 280 BsrBI CCG'CTC Cuts at: 1394 1823 3551 4353 1394 Size: 429 1728 802 3507 Fragments arranged by size: 3507 1728 802 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2745 3555 4961 5135 65 Size: 1183 1497 810 1406 174 1396 Fragments arranged by size: 1497 1406 1396 1183 810 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2344 5110 5297 1700 Size: 110 534 2766 187 2869 Fragments arranged by size: 2869 2766 534 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6466 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6466 BssSI C'ACGA_G Cuts at: 4526 5538 4526 Size: 1012 5454 BstAPI GCAn_nnn'nTGC Cuts at: 794 2870 794 Size: 2076 4390 BstBI TT'CG_AA Cuts at: 5270 5270 Size: 6466 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6466 BstXI CCAn_nnnn'nTGG Cuts at: 2652 2674 3647 2652 Size: 22 973 5471 Fragments arranged by size: 5471 973 22 BstYI r'GATC_y Cuts at: 1972 3796 4163 4548 4565 6017 6103 6115 6461 Size: 1824 367 385 17 1452 86 12 346 Cuts at: 6461 1972 Size: 1977 Fragments arranged by size: 1977 1824 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2630 2637 3737 2630 Size: 7 1100 5359 Fragments arranged by size: 5359 1100 7 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 2205 2437 2464 2651 2682 Size: 552 939 21 180 232 27 187 31 Cuts at: 2682 3154 3204 3257 3417 3646 513 Size: 472 50 53 160 229 3333 Fragments arranged by size: 3333 939 552 472 232 229 187 180 160 53 50 31 27 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2203 2488 3257 3319 487 Size: 683 56 977 285 769 62 3634 Fragments arranged by size: 3634 977 769 683 285 62 56 BtsI GCAGTG_nn' Cuts at: 3348 3982 4776 4796 3348 Size: 634 794 20 5018 Fragments arranged by size: 5018 794 634 20 ClaI AT'CG_AT Cuts at: 1554 3099 1554 Size: 1545 4921 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6431 DraI TTT'AAA Cuts at: 3770 3770 Size: 6466 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2156 3166 5473 809 Size: 507 840 1010 2307 1802 Fragments arranged by size: 2307 1802 1010 840 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2346 2467 2664 Size: 54 1425 50 275 18 516 121 197 Cuts at: 2664 2685 2784 2931 3103 3138 3552 3649 3913 Size: 21 99 147 172 35 414 97 264 Cuts at: 3913 4802 6431 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 516 414 275 264 197 172 147 121 99 97 54 50 43 35 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3913 1537 Size: 275 2101 4090 Fragments arranged by size: 4090 2101 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2612 3737 4396 1353 Size: 1259 1125 659 3423 Fragments arranged by size: 3423 1259 1125 659 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2286 3077 5053 5427 5573 387 Size: 1064 138 697 791 1976 374 146 1280 Fragments arranged by size: 1976 1280 1064 791 697 374 146 138 EcoICRI GAG'CTC Cuts at: 2434 3286 3368 3487 2434 Size: 852 82 119 5413 Fragments arranged by size: 5413 852 119 82 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2696 2837 2961 3023 3598 4529 Size: 393 539 525 141 124 62 575 931 Cuts at: 4529 5126 5913 6181 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 931 787 597 575 539 525 393 268 141 124 62 EcoO109I rG'GnC_Cy Cuts at: 2104 2406 2641 2779 3016 3055 3669 3711 2104 Size: 302 235 138 237 39 614 42 4859 Fragments arranged by size: 4859 614 302 237 235 138 42 39 EcoRI G'AATT_C Cuts at: 3775 4153 3775 Size: 378 6088 FspI TGC'GCA Cuts at: 4972 4972 Size: 6466 HaeII r_GCGC'y Cuts at: 1678 2063 3204 5613 1678 Size: 385 1141 2409 2531 Fragments arranged by size: 2531 2409 1141 385 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2166 2198 2265 2297 5107 Size: 32 135 32 594 32 67 32 2810 Cuts at: 5107 5139 5181 5213 1373 Size: 32 42 32 2626 Fragments arranged by size: 2810 2626 594 135 67 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2248 2592 3580 3810 4056 669 Size: 988 281 310 344 988 230 246 3079 Fragments arranged by size: 3079 988 988 344 310 281 246 230 HindIII A'AGCT_T Cuts at: 748 748 Size: 6466 HpaI GTT'AAC Cuts at: 4056 4056 Size: 6466 KpnI G_GTAC'C Cuts at: 3791 3791 Size: 6466 MfeI C'AATT_G Cuts at: 4065 4065 Size: 6466 MluI A'CGCG_T Cuts at: 6342 6342 Size: 6466 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5580 5764 1305 Size: 4275 184 2007 Fragments arranged by size: 4275 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2666 2687 2786 2933 3651 Size: 54 1425 343 834 21 99 147 718 Cuts at: 3651 10 Size: 2825 Fragments arranged by size: 2825 1425 834 718 343 147 99 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2650 4424 4783 4942 518 Size: 648 1484 1774 359 159 2042 Fragments arranged by size: 2042 1774 1484 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2488 2767 3882 4559 5707 5952 1606 Size: 882 279 1115 677 1148 245 2120 Fragments arranged by size: 2120 1148 1115 882 677 279 245 NaeI GCC'GGC Cuts at: 2346 2346 Size: 6466 NcoI C'CATG_G Cuts at: 513 1065 2004 2464 2651 3417 513 Size: 552 939 460 187 766 3562 Fragments arranged by size: 3562 939 766 552 460 187 NdeI CA'TA_TG Cuts at: 387 387 Size: 6466 NgoMIV G'CCGG_C Cuts at: 2344 2344 Size: 6466 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6466 NotI GC'GGCC_GC Cuts at: 3913 3913 Size: 6466 NspI r_CATG'y Cuts at: 1132 1229 2528 3578 3824 5369 1132 Size: 97 1299 1050 246 1545 2229 Fragments arranged by size: 2229 1545 1299 1050 246 97 PasI CC'CwG_GG Cuts at: 1348 2478 2827 3448 1348 Size: 1130 349 621 4366 Fragments arranged by size: 4366 1130 621 349 PciI A'CATG_T Cuts at: 5365 5365 Size: 6466 PflMI CCAn_nnn'nTGG Cuts at: 1108 2734 1108 Size: 1626 4840 PfoI T'CCnGG_A Cuts at: 3288 3370 3288 Size: 82 6384 PmeI GTTT'AAAC Cuts at: 3770 3770 Size: 6466 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4535 4567 6048 6080 4535 Size: 32 1481 32 4921 Fragments arranged by size: 4921 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2641 2779 3016 3055 2641 Size: 138 237 39 6052 Fragments arranged by size: 6052 237 138 39 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6466 PsiI TTA'TAA Cuts at: 4036 4036 Size: 6466 PspOMI G'GGCC_C Cuts at: 2406 2406 Size: 6466 PstI C_TGCA'G Cuts at: 830 2778 2866 3343 3818 830 Size: 1948 88 477 475 3478 Fragments arranged by size: 3478 1948 477 475 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6466 PvuII CAG'CTG Cuts at: 2488 2767 2488 Size: 279 6187 SacI G_AGCT'C Cuts at: 2436 3288 3370 3489 2436 Size: 852 82 119 5413 Fragments arranged by size: 5413 852 119 82 SalI G'TCGA_C Cuts at: 1936 3808 1936 Size: 1872 4594 SanDI GG'GwC_CC Cuts at: 3016 3016 Size: 6466 SapI GCTCTTCn'nnn_ Cuts at: 2612 2612 Size: 6466 SbfI CC_TGCA'GG Cuts at: 2866 3818 2866 Size: 952 5514 ScaI AGT'ACT Cuts at: 4714 6426 4714 Size: 1712 4754 SfcI C'TryA_G Cuts at: 826 1045 2774 2862 3130 3339 3814 4949 5630 Size: 219 1729 88 268 209 475 1135 681 Cuts at: 5630 5821 826 Size: 191 1471 Fragments arranged by size: 1729 1471 1135 681 475 268 219 209 191 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6466 SmaI CCC'GGG Cuts at: 1872 2012 2333 2428 3053 3314 3793 1872 Size: 140 321 95 625 261 479 4545 Fragments arranged by size: 4545 625 479 321 261 140 95 SmlI C'TyrA_G Cuts at: 820 1017 1943 2981 4570 5471 5733 820 Size: 197 926 1038 1589 901 262 1553 Fragments arranged by size: 1589 1553 1038 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4257 493 Size: 3764 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6466 SphI G_CATG'C Cuts at: 1132 3824 1132 Size: 2692 3774 SspI AAT'ATT Cuts at: 5 52 4390 5 Size: 47 4338 2081 Fragments arranged by size: 4338 2081 47 StuI AGG'CCT Cuts at: 1517 2123 2635 2938 3146 1517 Size: 606 512 303 208 4837 Fragments arranged by size: 4837 606 512 303 208 StyI C'CwwG_G Cuts at: 513 1065 2004 2154 2464 2651 2751 2991 3417 Size: 552 939 150 310 187 100 240 426 Cuts at: 3417 3905 513 Size: 488 3074 Fragments arranged by size: 3074 939 552 488 426 310 240 187 150 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3350 4493 4510 4663 4848 5293 325 Size: 1255 1770 1143 17 153 185 445 1498 Fragments arranged by size: 1770 1498 1255 1143 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2844 4712 6424 Size: 274 80 33 51 1443 867 1868 1712 Cuts at: 6424 96 Size: 138 Fragments arranged by size: 1868 1712 1443 867 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3231 3263 3342 3374 6426 6458 3231 Size: 32 79 32 3052 32 3239 Fragments arranged by size: 3239 3052 79 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6466 XbaI T'CTAG_A Cuts at: 3802 3802 Size: 6466 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2108 2224 2462 2695 3098 2108 Size: 116 238 233 403 5476 Fragments arranged by size: 5476 403 238 233 116 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6466 XmaI C'CCGG_G Cuts at: 1870 2010 2331 2426 3051 3312 3791 1870 Size: 140 321 95 625 261 479 4545 Fragments arranged by size: 4545 625 479 321 261 140 95 XmnI GAAnn'nnTTC Cuts at: 4595 4595 Size: 6466 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4274 276 Size: 53 83 186 936 2740 2468 Fragments arranged by size: 2740 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI