(Linear) (Six-base) MAPSORT of: KIEE5769.seq Check: 635 from: 1 to: 1761 KIEE5769.orf With 182 enzymes: SgfI * December 5, 2014 13:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1341 1761 Size: 1341 420 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 956 1593 1761 Size: 956 637 168 Fragments arranged by size: 956 637 168 AflIII A'CryG_T Cuts at: 0 950 1761 Size: 950 811 AlwNI CAG_nnn'CTG Cuts at: 0 384 729 777 1761 Size: 384 345 48 984 Fragments arranged by size: 984 384 345 48 ApaI G_GGCC'C Cuts at: 0 405 1761 Size: 405 1356 ApaLI G'TGCA_C Cuts at: 0 374 1761 Size: 374 1387 AvaI C'yCGr_G Cuts at: 0 5 326 354 421 793 1046 1307 1761 Size: 5 321 28 67 372 253 261 454 Fragments arranged by size: 454 372 321 261 253 67 28 5 BanI G'GyrC_C Cuts at: 0 336 417 816 865 1318 1416 1761 Size: 336 81 399 49 453 98 345 Fragments arranged by size: 453 399 345 336 98 81 49 BanII G_rGCy'C Cuts at: 0 196 405 431 1283 1365 1484 1604 1761 Size: 196 209 26 852 82 119 120 157 Fragments arranged by size: 852 209 196 157 120 119 82 26 BbsI GAAGACnn'nnnn_ Cuts at: 0 693 1761 Size: 693 1068 BbvCI CC'TCA_GC Cuts at: 0 538 1761 Size: 538 1223 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 219 253 1761 Size: 219 34 1508 Fragments arranged by size: 1508 219 34 BclI T'GATC_A Cuts at: 0 1430 1761 Size: 1430 331 BglI GCCn_nnn'nGGC Cuts at: 0 461 1761 Size: 461 1300 BlpI GC'TnA_GC Cuts at: 0 1117 1761 Size: 1117 644 Bme1580I G_kGCm'C Cuts at: 0 378 397 405 422 821 1421 1761 Size: 378 19 8 17 399 600 340 Fragments arranged by size: 600 399 378 340 19 17 8 BmgBI CAC'GTC Cuts at: 0 1163 1761 Size: 1163 598 BmrI ACTGGGnnnn_n' Cuts at: 0 399 400 1378 1761 Size: 399 1 978 383 Fragments arranged by size: 978 399 383 1 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 196 1761 Size: 196 1565 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 691 832 1018 1761 Size: 166 525 141 186 743 Fragments arranged by size: 743 525 186 166 141 Bpu10I CC'TnA_GC Cuts at: 0 538 1761 Size: 538 1223 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 961 1761 Size: 961 800 BsaI GGTCTCn'nnnn_ Cuts at: 0 667 1057 1761 Size: 667 390 704 Fragments arranged by size: 704 667 390 BsaHI Gr'CG_yC Cuts at: 0 583 1761 Size: 583 1178 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1226 1256 1337 1367 1761 Size: 1226 30 81 30 394 Fragments arranged by size: 1226 394 81 30 30 BseYI C'CCAG_C Cuts at: 0 39 717 1005 1056 1073 1179 1761 Size: 39 678 288 51 17 106 582 Fragments arranged by size: 678 582 288 106 51 39 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 213 535 638 1642 1761 Size: 213 322 103 1004 119 Fragments arranged by size: 1004 322 213 119 103 BsiHKAI G_wGCw'C Cuts at: 0 378 431 1283 1365 1484 1761 Size: 378 53 852 82 119 277 Fragments arranged by size: 852 378 277 119 82 53 BsmI GAATG_Cn' Cuts at: 0 1044 1761 Size: 1044 717 BsmBI CGTCTCn'nnnn_ Cuts at: 0 325 964 1728 1761 Size: 325 639 764 33 Fragments arranged by size: 764 639 325 33 Bsp1286I G_dGCh'C Cuts at: 0 196 378 397 405 422 431 821 1283 Size: 196 182 19 8 17 9 390 462 Cuts at: 1283 1365 1421 1484 1604 1761 Size: 82 56 63 120 157 Fragments arranged by size: 462 390 196 182 157 120 82 63 56 19 17 9 8 BspHI T'CATG_A Cuts at: 0 1511 1761 Size: 1511 250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1061 1341 1761 Size: 1061 280 420 Fragments arranged by size: 1061 420 280 BsrBI CCG'CTC Cuts at: 0 1546 1761 Size: 1546 215 BsrDI GCAATG_nn' Cuts at: 0 740 1550 1761 Size: 740 810 211 Fragments arranged by size: 810 740 211 BsrFI r'CCGG_y Cuts at: 0 339 1761 Size: 339 1422 BstAPI GCAn_nnn'nTGC Cuts at: 0 865 1761 Size: 865 896 BstXI CCAn_nnnn'nTGG Cuts at: 0 647 669 1642 1761 Size: 647 22 973 119 Fragments arranged by size: 973 647 119 22 Bsu36I CC'TnA_GG Cuts at: 0 625 632 1732 1761 Size: 625 7 1100 29 Fragments arranged by size: 1100 625 29 7 BtgI C'CryG_G Cuts at: 0 20 200 432 459 646 677 1149 1199 Size: 20 180 232 27 187 31 472 50 Cuts at: 1199 1252 1412 1641 1761 Size: 53 160 229 120 Fragments arranged by size: 472 232 229 187 180 160 120 53 50 31 27 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 198 483 1252 1314 1761 Size: 198 285 769 62 447 Fragments arranged by size: 769 447 285 198 62 BtsI GCAGTG_nn' Cuts at: 0 1343 1761 Size: 1343 418 ClaI AT'CG_AT Cuts at: 0 1094 1761 Size: 1094 667 DrdI GACnn_nn'nnGTC Cuts at: 0 151 1161 1761 Size: 151 1010 600 Fragments arranged by size: 1010 600 151 EaeI y'GGCC_r Cuts at: 0 341 462 659 680 779 926 1098 1133 Size: 341 121 197 21 99 147 172 35 Cuts at: 1133 1547 1644 1761 Size: 414 97 117 Fragments arranged by size: 414 341 197 172 147 121 117 99 97 35 21 EarI CTCTTCn'nnn_ Cuts at: 0 607 1732 1761 Size: 607 1125 29 Fragments arranged by size: 1125 607 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 281 1072 1761 Size: 281 791 689 Fragments arranged by size: 791 689 281 EcoICRI GAG'CTC Cuts at: 0 429 1281 1363 1482 1761 Size: 429 852 82 119 279 Fragments arranged by size: 852 429 279 119 82 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 691 832 956 1018 1593 1761 Size: 166 525 141 124 62 575 168 Fragments arranged by size: 575 525 168 166 141 124 62 EcoO109I rG'GnC_Cy Cuts at: 0 99 401 636 774 1011 1050 1664 1706 Size: 99 302 235 138 237 39 614 42 Cuts at: 1706 1761 Size: 55 Fragments arranged by size: 614 302 237 235 138 99 55 42 39 HaeII r_GCGC'y Cuts at: 0 58 1199 1761 Size: 58 1141 562 Fragments arranged by size: 1141 562 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 161 193 260 292 1761 Size: 161 32 67 32 1469 Fragments arranged by size: 1469 161 67 32 32 HincII GTy'rAC Cuts at: 0 243 587 1575 1761 Size: 243 344 988 186 Fragments arranged by size: 988 344 243 186 MscI TGG'CCA Cuts at: 0 661 682 781 928 1646 1761 Size: 661 21 99 147 718 115 Fragments arranged by size: 718 661 147 115 99 21 MslI CAynn'nnrTG Cuts at: 0 645 1761 Size: 645 1116 MspA1I CmG'CkG Cuts at: 0 483 762 1761 Size: 483 279 999 Fragments arranged by size: 999 483 279 NaeI GCC'GGC Cuts at: 0 341 1761 Size: 341 1420 NcoI C'CATG_G Cuts at: 0 459 646 1412 1761 Size: 459 187 766 349 Fragments arranged by size: 766 459 349 187 NgoMIV G'CCGG_C Cuts at: 0 339 1761 Size: 339 1422 NspI r_CATG'y Cuts at: 0 523 1573 1761 Size: 523 1050 188 Fragments arranged by size: 1050 523 188 PasI CC'CwG_GG Cuts at: 0 473 822 1443 1761 Size: 473 349 621 318 Fragments arranged by size: 621 473 349 318 PflMI CCAn_nnn'nTGG Cuts at: 0 729 1761 Size: 729 1032 PfoI T'CCnGG_A Cuts at: 0 1283 1365 1761 Size: 1283 82 396 Fragments arranged by size: 1283 396 82 PpuMI rG'GwC_Cy Cuts at: 0 636 774 1011 1050 1761 Size: 636 138 237 39 711 Fragments arranged by size: 711 636 237 138 39 PspOMI G'GGCC_C Cuts at: 0 401 1761 Size: 401 1360 PstI C_TGCA'G Cuts at: 0 773 861 1338 1761 Size: 773 88 477 423 Fragments arranged by size: 773 477 423 88 PvuII CAG'CTG Cuts at: 0 483 762 1761 Size: 483 279 999 Fragments arranged by size: 999 483 279 SacI G_AGCT'C Cuts at: 0 431 1283 1365 1484 1761 Size: 431 852 82 119 277 Fragments arranged by size: 852 431 277 119 82 SanDI GG'GwC_CC Cuts at: 0 1011 1761 Size: 1011 750 SapI GCTCTTCn'nnn_ Cuts at: 0 607 1761 Size: 607 1154 SbfI CC_TGCA'GG Cuts at: 0 861 1761 Size: 861 900 SfcI C'TryA_G Cuts at: 0 769 857 1125 1334 1761 Size: 769 88 268 209 427 Fragments arranged by size: 769 427 268 209 88 SmaI CCC'GGG Cuts at: 0 7 328 423 1048 1309 1761 Size: 7 321 95 625 261 452 Fragments arranged by size: 625 452 321 261 95 7 SmlI C'TyrA_G Cuts at: 0 976 1761 Size: 976 785 StuI AGG'CCT Cuts at: 0 118 630 933 1141 1761 Size: 118 512 303 208 620 Fragments arranged by size: 620 512 303 208 118 StyI C'CwwG_G Cuts at: 0 149 459 646 746 986 1412 1761 Size: 149 310 187 100 240 426 349 Fragments arranged by size: 426 349 310 240 187 149 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1345 1761 Size: 1345 416 TatI w'GTAC_w Cuts at: 0 839 1761 Size: 839 922 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1226 1258 1337 1369 1761 Size: 1226 32 79 32 392 Fragments arranged by size: 1226 392 79 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 103 219 457 690 1093 1761 Size: 103 116 238 233 403 668 Fragments arranged by size: 668 403 238 233 116 103 XmaI C'CCGG_G Cuts at: 0 5 326 421 1046 1307 1761 Size: 5 321 95 625 261 454 Fragments arranged by size: 625 454 321 261 95 5 Enzymes that do cut: AarI AcuI AflIII AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI SbfI SfcI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BglII BmtI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BspEI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I CspCI DraI DraIII EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI