(Circular) (Six-base) MAPSORT of: pFN21AE9690.seq Check: 6118 from: 1 to: 7660 pFN21AE9690 7660 bp With 182 enzymes: SgfI * December 8, 2014 13:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4849 5470 278 Size: 53 83 186 936 3313 621 2468 Fragments arranged by size: 3313 2468 936 621 186 83 53 AccI GT'mk_AC Cuts at: 1937 3928 5003 1937 Size: 1991 1075 4594 Fragments arranged by size: 4594 1991 1075 Acc65I G'GTAC_C Cuts at: 3671 4981 3671 Size: 1310 6350 AclI AA'CG_TT Cuts at: 1563 5787 6160 1563 Size: 4224 373 3063 Fragments arranged by size: 4224 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2451 2838 3267 3309 5723 7107 7375 1632 Size: 819 387 429 42 2414 1384 268 1917 Fragments arranged by size: 2414 1917 1384 819 429 387 268 42 AfeI AGC'GCT Cuts at: 3229 3229 Size: 7660 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7463 AflIII A'CryG_T Cuts at: 1203 6559 7536 1203 Size: 5356 977 1327 Fragments arranged by size: 5356 1327 977 AgeI A'CCGG_T Cuts at: 6491 6491 Size: 7660 AhdI GACnn_n'nnGTC Cuts at: 6389 6389 Size: 7660 AleI CACnn'nnGTG Cuts at: 1166 2205 1166 Size: 1039 6621 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7660 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3040 3072 3040 Size: 32 7628 AlwNI CAG_nnn'CTG Cuts at: 2576 4234 5403 6975 2576 Size: 1658 1169 1572 3261 Fragments arranged by size: 3261 1658 1572 1169 ApaLI G'TGCA_C Cuts at: 2538 5717 6873 2538 Size: 3179 1156 3325 Fragments arranged by size: 3325 3179 1156 ApoI r'AATT_y Cuts at: 1493 2192 4969 5195 5347 7456 1493 Size: 699 2777 226 152 2109 1697 Fragments arranged by size: 2777 2109 1697 699 226 152 AseI AT'TA_AT Cuts at: 160 1032 2172 2702 2955 6214 160 Size: 872 1140 530 253 3259 1606 Fragments arranged by size: 3259 1606 1140 872 530 253 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7660 AvaI C'yCGr_G Cuts at: 1870 1943 3792 4985 1870 Size: 73 1849 1193 4545 Fragments arranged by size: 4545 1849 1193 73 AvrII C'CTAG_G Cuts at: 2368 2368 Size: 7660 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3171 3204 3703 3736 3171 Size: 33 499 33 7095 Fragments arranged by size: 7095 499 33 33 BamHI G'GATC_C Cuts at: 2109 4990 5357 2109 Size: 2881 367 4412 Fragments arranged by size: 4412 2881 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3671 4117 4981 6436 618 Size: 325 217 627 1884 446 864 1455 1842 Fragments arranged by size: 1884 1842 1455 864 627 446 325 217 BanII G_rGCy'C Cuts at: 1391 2037 2130 4540 4979 1391 Size: 646 93 2410 439 4072 Fragments arranged by size: 4072 2410 646 439 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4791 4841 928 Size: 971 2892 50 3747 Fragments arranged by size: 3747 2892 971 50 BbvCI CC'TCA_GC Cuts at: 2786 4164 2786 Size: 1378 6282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5851 5885 1903 Size: 34 3914 34 3678 Fragments arranged by size: 3914 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5552 6762 5552 Size: 1210 6450 BclI T'GATC_A Cuts at: 1548 1548 Size: 7660 BfrBI ATG'CAT Cuts at: 3491 3491 Size: 7660 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3130 6271 136 Size: 107 122 71 631 605 1458 3141 1525 Fragments arranged by size: 3141 1525 1458 631 605 122 107 71 BglII A'GATC_T Cuts at: 3038 7655 3038 Size: 4617 3043 BlpI GC'TnA_GC Cuts at: 5077 5077 Size: 7660 Bme1580I G_kGCm'C Cuts at: 1790 2542 4306 4916 5721 6877 1790 Size: 752 1764 610 805 1156 2573 Fragments arranged by size: 2573 1764 1156 805 752 610 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7660 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3578 4439 5391 Size: 416 53 83 399 24 2161 861 952 Cuts at: 5391 6349 442 Size: 958 1753 Fragments arranged by size: 2161 1753 958 952 861 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7660 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3286 3286 Size: 7660 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3019 4206 4392 4464 6320 1239 Size: 1780 1187 186 72 1856 2579 Fragments arranged by size: 2579 1856 1780 1187 186 72 Bpu10I CC'TnA_GC Cuts at: 2786 3707 4164 2786 Size: 921 457 6282 Fragments arranged by size: 6282 921 457 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3148 3361 4023 5785 6650 6948 3148 Size: 213 662 1762 865 298 3860 Fragments arranged by size: 3860 1762 865 662 298 213 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2550 3018 6323 882 Size: 617 1051 468 3305 2219 Fragments arranged by size: 3305 2219 1051 617 468 BsaAI yAC'GTr Cuts at: 493 1204 5451 493 Size: 711 4247 2702 Fragments arranged by size: 4247 2702 711 BsaBI GATnn'nnATC Cuts at: 7654 7654 Size: 7660 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4846 5467 5849 6484 Size: 53 83 186 936 3313 621 382 635 Cuts at: 6484 275 Size: 1451 Fragments arranged by size: 3313 1451 936 635 621 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6093 6491 6765 6912 7323 6093 Size: 398 274 147 411 6430 Fragments arranged by size: 6430 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3040 3070 3400 3430 4015 Size: 30 165 30 1637 30 330 30 585 Cuts at: 4015 4045 4204 4234 1178 Size: 30 159 30 4604 Fragments arranged by size: 4604 1637 585 330 165 159 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4008 4291 4500 1186 Size: 2822 283 209 4346 Fragments arranged by size: 4346 2822 283 209 BseYI C'CCAG_C Cuts at: 3450 3574 3888 3894 4941 6863 7359 3450 Size: 124 314 6 1047 1922 496 3751 Fragments arranged by size: 3751 1922 1047 496 314 124 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 4137 4733 1163 Size: 573 603 1798 596 4090 Fragments arranged by size: 4090 1798 603 596 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5110 5871 6899 1540 Size: 275 186 3109 761 1028 2301 Fragments arranged by size: 3109 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2037 2542 4540 5721 5806 6877 2037 Size: 505 1998 1181 85 1071 2820 Fragments arranged by size: 2820 1998 1181 1071 505 85 BsmI GAATG_Cn' Cuts at: 2521 3142 3997 5171 5264 2521 Size: 621 855 1174 93 4917 Fragments arranged by size: 4917 1174 855 621 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4837 4837 Size: 7660 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2130 2542 4306 4540 4916 4979 Size: 399 247 93 412 1764 234 376 63 Cuts at: 4979 5721 5806 6877 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1764 1071 742 412 399 376 247 234 93 85 63 BspEI T'CCGG_A Cuts at: 7323 7323 Size: 7660 BspHI T'CATG_A Cuts at: 3682 4243 5549 7279 3682 Size: 561 1306 1730 4063 Fragments arranged by size: 4063 1730 1306 561 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5015 844 Size: 4171 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5547 1394 Size: 429 3724 3507 Fragments arranged by size: 3724 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3429 3692 4475 4681 6155 6329 65 Size: 1183 2181 263 783 206 1474 174 1396 Fragments arranged by size: 2181 1474 1396 1183 783 263 206 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6304 6491 1700 Size: 110 4494 187 2869 Fragments arranged by size: 4494 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4066 4715 96 Size: 3970 649 3041 Fragments arranged by size: 3970 3041 649 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7660 BssSI C'ACGA_G Cuts at: 5720 6732 5720 Size: 1012 6648 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7660 BstBI TT'CG_AA Cuts at: 6464 6464 Size: 7660 BstEII G'GTnAC_C Cuts at: 1184 2243 3432 3530 1184 Size: 1059 1189 98 5314 Fragments arranged by size: 5314 1189 1059 98 BstXI CCAn_nnnn'nTGG Cuts at: 2303 2303 Size: 7660 BstYI r'GATC_y Cuts at: 1972 2109 3038 4990 5357 5742 5759 7211 7297 Size: 137 929 1952 367 385 17 1452 86 Cuts at: 7297 7309 7655 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1952 1452 929 385 367 346 137 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2881 2881 Size: 7660 BtgI C'CryG_G Cuts at: 513 1065 3089 513 Size: 552 2024 5084 Fragments arranged by size: 5084 2024 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6921 Fragments arranged by size: 6921 683 56 BtsI GCAGTG_nn' Cuts at: 2797 4750 5176 5970 5990 2797 Size: 1953 426 794 20 4467 Fragments arranged by size: 4467 1953 794 426 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7660 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7625 DraI TTT'AAA Cuts at: 4964 4964 Size: 7660 DraIII CAC_nnn'GTG Cuts at: 2381 3464 3650 4472 4876 2381 Size: 1083 186 822 404 5165 Fragments arranged by size: 5165 1083 822 404 186 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6667 809 Size: 507 5351 1802 Fragments arranged by size: 5351 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3077 3808 5107 Size: 54 1425 50 275 18 1247 731 1299 Cuts at: 5107 5996 7625 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1299 1247 889 731 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5107 1537 Size: 275 3295 4090 Fragments arranged by size: 4090 3295 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2806 4545 5590 1353 Size: 701 752 1739 1045 3423 Fragments arranged by size: 3423 1739 1045 752 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4023 6247 6621 6767 387 Size: 1064 138 2434 2224 374 146 1280 Fragments arranged by size: 2434 2224 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2035 4538 2035 Size: 2503 5157 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2451 2838 3019 3267 3309 4206 4392 Size: 393 819 387 181 248 42 897 186 Cuts at: 4392 4464 5723 6320 7107 7375 1239 Size: 72 1259 597 787 268 1524 Fragments arranged by size: 1524 1259 897 819 787 597 393 387 268 248 186 181 72 42 EcoNI CCTnn'n_nnAGG Cuts at: 2671 2671 Size: 7660 EcoO109I rG'GnC_Cy Cuts at: 4622 4622 Size: 7660 EcoRI G'AATT_C Cuts at: 2192 4969 5347 2192 Size: 2777 378 4505 Fragments arranged by size: 4505 2777 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2129 3453 3858 2129 Size: 1324 405 5931 Fragments arranged by size: 5931 1324 405 FspI TGC'GCA Cuts at: 3882 6166 3882 Size: 2284 5376 HaeII r_GCGC'y Cuts at: 1678 3231 6807 1678 Size: 1553 3576 2531 Fragments arranged by size: 3576 2531 1553 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2558 2590 3786 3818 4015 Size: 32 135 32 986 32 1196 32 197 Cuts at: 4015 4047 4222 4254 4276 4308 4595 4627 6301 Size: 32 175 32 22 32 287 32 1674 Cuts at: 6301 6333 6375 6407 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1674 1196 986 287 197 175 135 42 32 32 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3839 3929 4885 5004 5250 669 Size: 988 281 1901 90 956 119 246 3079 Fragments arranged by size: 3079 1901 988 956 281 246 119 90 HindIII A'AGCT_T Cuts at: 748 2916 3981 748 Size: 2168 1065 4427 Fragments arranged by size: 4427 2168 1065 HpaI GTT'AAC Cuts at: 5250 5250 Size: 7660 KpnI G_GTAC'C Cuts at: 3675 4985 3675 Size: 1310 6350 MfeI C'AATT_G Cuts at: 4704 5259 4704 Size: 555 7105 MluI A'CGCG_T Cuts at: 7536 7536 Size: 7660 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2977 4319 6774 6958 1305 Size: 1672 1342 2455 184 2007 Fragments arranged by size: 2455 2007 1672 1342 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3810 10 Size: 54 1425 343 1978 3860 Fragments arranged by size: 3860 1978 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2205 3735 4002 4242 4368 5618 5977 Size: 648 1039 1530 267 240 126 1250 359 Cuts at: 5977 6136 518 Size: 159 2042 Fragments arranged by size: 2042 1530 1250 1039 648 359 267 240 159 126 MspA1I CmG'CkG Cuts at: 1606 2873 3287 3578 3821 4151 4945 5076 5753 Size: 1267 414 291 243 330 794 131 677 Cuts at: 5753 6901 7146 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1267 1148 794 677 414 330 291 245 243 131 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7108 NdeI CA'TA_TG Cuts at: 387 4919 387 Size: 4532 3128 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7660 NotI GC'GGCC_GC Cuts at: 5107 5107 Size: 7660 NsiI A_TGCA'T Cuts at: 3493 3493 Size: 7660 NspI r_CATG'y Cuts at: 1132 1229 3940 4073 5018 6563 1132 Size: 97 2711 133 945 1545 2229 Fragments arranged by size: 2711 2229 1545 945 133 97 PasI CC'CwG_GG Cuts at: 1348 4696 1348 Size: 3348 4312 PciI A'CATG_T Cuts at: 6559 6559 Size: 7660 PflMI CCAn_nnn'nTGG Cuts at: 1108 4349 1108 Size: 3241 4419 PmeI GTTT'AAAC Cuts at: 4964 4964 Size: 7660 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3040 3072 5729 5761 7242 7274 3040 Size: 32 2657 32 1481 32 3426 Fragments arranged by size: 3426 2657 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4622 4622 Size: 7660 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7660 PsiI TTA'TAA Cuts at: 5230 5230 Size: 7660 PstI C_TGCA'G Cuts at: 830 2158 4456 5012 830 Size: 1328 2298 556 3478 Fragments arranged by size: 3478 2298 1328 556 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7660 PvuII CAG'CTG Cuts at: 2873 3287 3578 4151 4945 2873 Size: 414 291 573 794 5588 Fragments arranged by size: 5588 794 573 414 291 SacI G_AGCT'C Cuts at: 2037 4540 2037 Size: 2503 5157 SalI G'TCGA_C Cuts at: 1936 3927 5002 1936 Size: 1991 1075 4594 Fragments arranged by size: 4594 1991 1075 SapI GCTCTTCn'nnn_ Cuts at: 4545 4545 Size: 7660 SbfI CC_TGCA'GG Cuts at: 2158 5012 2158 Size: 2854 4806 ScaI AGT'ACT Cuts at: 5908 7620 5908 Size: 1712 5948 SexAI A'CCwGG_T Cuts at: 4468 4468 Size: 7660 SfcI C'TryA_G Cuts at: 826 1045 2154 3183 3363 4452 5008 6143 6824 Size: 219 1109 1029 180 1089 556 1135 681 Cuts at: 6824 7015 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1109 1089 1029 681 556 219 191 180 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7660 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7660 SmaI CCC'GGG Cuts at: 1872 4987 1872 Size: 3115 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3127 3376 4038 5764 6665 6927 Size: 197 926 1184 249 662 1726 901 262 Cuts at: 6927 820 Size: 1553 Fragments arranged by size: 1726 1553 1184 926 901 662 262 249 197 SnaBI TAC'GTA Cuts at: 493 5451 493 Size: 4958 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7660 SphI G_CATG'C Cuts at: 1132 5018 1132 Size: 3886 3774 SspI AAT'ATT Cuts at: 5 52 2349 5584 5 Size: 47 2297 3235 2081 Fragments arranged by size: 3235 2297 2081 47 StuI AGG'CCT Cuts at: 1517 3269 1517 Size: 1752 5908 StyI C'CwwG_G Cuts at: 513 1065 2368 3025 3428 5099 513 Size: 552 1303 657 403 1671 3074 Fragments arranged by size: 3074 1671 1303 657 552 403 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3000 3588 4356 5687 5704 5857 6042 Size: 1255 1420 588 768 1331 17 153 185 Cuts at: 6042 6487 325 Size: 445 1498 Fragments arranged by size: 1498 1420 1331 1255 768 588 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3637 4066 4145 Size: 274 80 33 51 1443 1660 429 79 Cuts at: 4145 4715 4802 5906 7618 96 Size: 570 87 1104 1712 138 Fragments arranged by size: 1712 1660 1443 1104 570 429 274 138 87 80 79 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2365 2397 4204 4236 7620 7652 2365 Size: 32 1807 32 3384 32 2373 Fragments arranged by size: 3384 2373 1807 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4384 1316 Size: 3068 4592 XbaI T'CTAG_A Cuts at: 4996 4996 Size: 7660 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4924 4924 Size: 7660 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7660 XmaI C'CCGG_G Cuts at: 1870 4985 1870 Size: 3115 4545 XmnI GAAnn'nnTTC Cuts at: 2192 4650 5789 2192 Size: 2458 1139 4063 Fragments arranged by size: 4063 2458 1139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4847 5468 276 Size: 53 83 186 936 3313 621 2468 Fragments arranged by size: 3313 2468 936 621 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BbeI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI