(Linear) (Six-base) MAPSORT of: KIEE9690.seq Check: 6334 from: 1 to: 2955 KIEE9690.orf With 182 enzymes: SgfI * December 5, 2014 13:30 .. AatII G_ACGT'C Cuts at: 0 2844 2955 Size: 2844 111 AccI GT'mk_AC Cuts at: 0 1923 2955 Size: 1923 1032 Acc65I G'GTAC_C Cuts at: 0 1666 2955 Size: 1666 1289 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 446 833 1262 1304 2955 Size: 446 387 429 42 1651 Fragments arranged by size: 1651 446 429 387 42 AfeI AGC'GCT Cuts at: 0 1224 2955 Size: 1224 1731 AleI CACnn'nnGTG Cuts at: 0 200 2955 Size: 200 2755 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1035 1067 2955 Size: 1035 32 1888 Fragments arranged by size: 1888 1035 32 AlwNI CAG_nnn'CTG Cuts at: 0 571 2229 2955 Size: 571 1658 726 Fragments arranged by size: 1658 726 571 ApaLI G'TGCA_C Cuts at: 0 533 2955 Size: 533 2422 ApoI r'AATT_y Cuts at: 0 187 2955 Size: 187 2768 AseI AT'TA_AT Cuts at: 0 167 697 950 2955 Size: 167 530 253 2005 Fragments arranged by size: 2005 530 253 167 AvaI C'yCGr_G Cuts at: 0 1787 2955 Size: 1787 1168 AvrII C'CTAG_G Cuts at: 0 363 2955 Size: 363 2592 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1166 1199 1698 1731 2955 Size: 1166 33 499 33 1224 Fragments arranged by size: 1224 1166 499 33 33 BamHI G'GATC_C Cuts at: 0 104 2955 Size: 104 2851 BanI G'GyrC_C Cuts at: 0 1666 2112 2955 Size: 1666 446 843 Fragments arranged by size: 1666 843 446 BanII G_rGCy'C Cuts at: 0 32 125 2535 2955 Size: 32 93 2410 420 Fragments arranged by size: 2410 420 93 32 BbsI GAAGACnn'nnnn_ Cuts at: 0 2786 2836 2955 Size: 2786 50 119 Fragments arranged by size: 2786 119 50 BbvCI CC'TCA_GC Cuts at: 0 781 2159 2955 Size: 781 1378 796 Fragments arranged by size: 1378 796 781 BfrBI ATG'CAT Cuts at: 0 1486 2955 Size: 1486 1469 BglI GCCn_nnn'nGGC Cuts at: 0 1125 2955 Size: 1125 1830 BglII A'GATC_T Cuts at: 0 1033 2955 Size: 1033 1922 Bme1580I G_kGCm'C Cuts at: 0 537 2301 2911 2955 Size: 537 1764 610 44 Fragments arranged by size: 1764 610 537 44 BmrI ACTGGGnnnn_n' Cuts at: 0 1573 2434 2955 Size: 1573 861 521 Fragments arranged by size: 1573 861 521 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1281 2955 Size: 1281 1674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1014 2201 2387 2459 2955 Size: 1014 1187 186 72 496 Fragments arranged by size: 1187 1014 496 186 72 Bpu10I CC'TnA_GC Cuts at: 0 781 1702 2159 2955 Size: 781 921 457 796 Fragments arranged by size: 921 796 781 457 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1143 1356 2018 2955 Size: 1143 213 662 937 Fragments arranged by size: 1143 937 662 213 BsaI GGTCTCn'nnnn_ Cuts at: 0 545 1013 2955 Size: 545 468 1942 Fragments arranged by size: 1942 545 468 BsaHI Gr'CG_yC Cuts at: 0 2841 2955 Size: 2841 114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1035 1065 1395 1425 2010 2040 2199 2229 Size: 1035 30 330 30 585 30 159 30 Cuts at: 2229 2955 Size: 726 Fragments arranged by size: 1035 726 585 330 159 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2003 2286 2495 2955 Size: 2003 283 209 460 Fragments arranged by size: 2003 460 283 209 BseYI C'CCAG_C Cuts at: 0 1445 1569 1883 1889 2936 2955 Size: 1445 124 314 6 1047 19 Fragments arranged by size: 1445 1047 314 124 19 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 2132 2728 2955 Size: 334 1798 596 227 Fragments arranged by size: 1798 596 334 227 BsiHKAI G_wGCw'C Cuts at: 0 32 537 2535 2955 Size: 32 505 1998 420 Fragments arranged by size: 1998 505 420 32 BsmI GAATG_Cn' Cuts at: 0 516 1137 1992 2955 Size: 516 621 855 963 Fragments arranged by size: 963 855 621 516 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2832 2955 Size: 2832 123 Bsp1286I G_dGCh'C Cuts at: 0 32 125 537 2301 2535 2911 2955 Size: 32 93 412 1764 234 376 44 Fragments arranged by size: 1764 412 376 234 93 44 32 BspHI T'CATG_A Cuts at: 0 1677 2238 2955 Size: 1677 561 717 Fragments arranged by size: 1677 717 561 BsrDI GCAATG_nn' Cuts at: 0 1424 1687 2470 2676 2955 Size: 1424 263 783 206 279 Fragments arranged by size: 1424 783 279 263 206 BsrGI T'GTAC_A Cuts at: 0 2061 2710 2955 Size: 2061 649 245 Fragments arranged by size: 2061 649 245 BstEII G'GTnAC_C Cuts at: 0 238 1427 1525 2955 Size: 238 1189 98 1430 Fragments arranged by size: 1430 1189 238 98 BstXI CCAn_nnnn'nTGG Cuts at: 0 298 2955 Size: 298 2657 BstYI r'GATC_y Cuts at: 0 104 1033 2955 Size: 104 929 1922 Fragments arranged by size: 1922 929 104 Bsu36I CC'TnA_GG Cuts at: 0 876 2955 Size: 876 2079 BtgI C'CryG_G Cuts at: 0 1084 2955 Size: 1084 1871 BtsI GCAGTG_nn' Cuts at: 0 792 2745 2955 Size: 792 1953 210 Fragments arranged by size: 1953 792 210 DraIII CAC_nnn'GTG Cuts at: 0 376 1459 1645 2467 2871 2955 Size: 376 1083 186 822 404 84 Fragments arranged by size: 1083 822 404 376 186 84 EaeI y'GGCC_r Cuts at: 0 1072 1803 2955 Size: 1072 731 1152 Fragments arranged by size: 1152 1072 731 EarI CTCTTCn'nnn_ Cuts at: 0 49 801 2540 2955 Size: 49 752 1739 415 Fragments arranged by size: 1739 752 415 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2018 2955 Size: 2018 937 EcoICRI GAG'CTC Cuts at: 0 30 2533 2955 Size: 30 2503 422 Fragments arranged by size: 2503 422 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 446 833 1014 1262 1304 2201 2387 2459 Size: 446 387 181 248 42 897 186 72 Cuts at: 2459 2955 Size: 496 Fragments arranged by size: 897 496 446 387 248 186 181 72 42 EcoNI CCTnn'n_nnAGG Cuts at: 0 666 2955 Size: 666 2289 EcoO109I rG'GnC_Cy Cuts at: 0 2617 2955 Size: 2617 338 EcoRI G'AATT_C Cuts at: 0 187 2955 Size: 187 2768 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 124 1448 1853 2955 Size: 124 1324 405 1102 Fragments arranged by size: 1324 1102 405 124 FspI TGC'GCA Cuts at: 0 1877 2955 Size: 1877 1078 HaeII r_GCGC'y Cuts at: 0 1226 2955 Size: 1226 1729 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 553 585 1781 1813 2010 2042 2217 2249 Size: 553 32 1196 32 197 32 175 32 Cuts at: 2249 2271 2303 2590 2622 2955 Size: 22 32 287 32 333 Fragments arranged by size: 1196 553 333 287 197 175 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 0 1834 1924 2880 2955 Size: 1834 90 956 75 Fragments arranged by size: 1834 956 90 75 HindIII A'AGCT_T Cuts at: 0 911 1976 2955 Size: 911 1065 979 Fragments arranged by size: 1065 979 911 KpnI G_GTAC'C Cuts at: 0 1670 2955 Size: 1670 1285 MfeI C'AATT_G Cuts at: 0 2699 2955 Size: 2699 256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 972 2314 2955 Size: 972 1342 641 Fragments arranged by size: 1342 972 641 MscI TGG'CCA Cuts at: 0 1805 2955 Size: 1805 1150 MslI CAynn'nnrTG Cuts at: 0 200 1730 1997 2237 2363 2955 Size: 200 1530 267 240 126 592 Fragments arranged by size: 1530 592 267 240 200 126 MspA1I CmG'CkG Cuts at: 0 868 1282 1573 1816 2146 2940 2955 Size: 868 414 291 243 330 794 15 Fragments arranged by size: 868 794 414 330 291 243 15 NdeI CA'TA_TG Cuts at: 0 2914 2955 Size: 2914 41 NsiI A_TGCA'T Cuts at: 0 1488 2955 Size: 1488 1467 NspI r_CATG'y Cuts at: 0 1935 2068 2955 Size: 1935 133 887 Fragments arranged by size: 1935 887 133 PasI CC'CwG_GG Cuts at: 0 2691 2955 Size: 2691 264 PflMI CCAn_nnn'nTGG Cuts at: 0 2344 2955 Size: 2344 611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1035 1067 2955 Size: 1035 32 1888 Fragments arranged by size: 1888 1035 32 PpuMI rG'GwC_Cy Cuts at: 0 2617 2955 Size: 2617 338 PstI C_TGCA'G Cuts at: 0 153 2451 2955 Size: 153 2298 504 Fragments arranged by size: 2298 504 153 PvuII CAG'CTG Cuts at: 0 868 1282 1573 2146 2940 2955 Size: 868 414 291 573 794 15 Fragments arranged by size: 868 794 573 414 291 15 SacI G_AGCT'C Cuts at: 0 32 2535 2955 Size: 32 2503 420 Fragments arranged by size: 2503 420 32 SalI G'TCGA_C Cuts at: 0 1922 2955 Size: 1922 1033 SapI GCTCTTCn'nnn_ Cuts at: 0 2540 2955 Size: 2540 415 SbfI CC_TGCA'GG Cuts at: 0 153 2955 Size: 153 2802 SexAI A'CCwGG_T Cuts at: 0 2463 2955 Size: 2463 492 SfcI C'TryA_G Cuts at: 0 149 1178 1358 2447 2955 Size: 149 1029 180 1089 508 Fragments arranged by size: 1089 1029 508 180 149 SmlI C'TyrA_G Cuts at: 0 1122 1371 2033 2955 Size: 1122 249 662 922 Fragments arranged by size: 1122 922 662 249 SspI AAT'ATT Cuts at: 0 344 2955 Size: 344 2611 StuI AGG'CCT Cuts at: 0 1264 2955 Size: 1264 1691 StyI C'CwwG_G Cuts at: 0 363 1020 1423 2955 Size: 363 657 403 1532 Fragments arranged by size: 1532 657 403 363 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 995 1583 2351 2955 Size: 995 588 768 604 Fragments arranged by size: 995 768 604 588 TatI w'GTAC_w Cuts at: 0 1632 2061 2140 2710 2797 2955 Size: 1632 429 79 570 87 158 Fragments arranged by size: 1632 570 429 158 87 79 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 360 392 2199 2231 2955 Size: 360 32 1807 32 724 Fragments arranged by size: 1807 724 360 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2379 2955 Size: 2379 576 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2919 2955 Size: 2919 36 XmnI GAAnn'nnTTC Cuts at: 0 187 2645 2955 Size: 187 2458 310 Fragments arranged by size: 2458 310 187 ZraI GAC'GTC Cuts at: 0 2842 2955 Size: 2842 113 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AleI AloI AlwNI ApaLI ApoI AseI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BfrBI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BsrGI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NdeI NsiI NspI PasI PflMI PpiI PpuMI PstI PvuII SacI SalI SapI SbfI SexAI SfcI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI ZraI Enzymes that do not cut: AarI AclI AflII AflIII AgeI AhdI AlfI ApaI AscI AsiSI BbeI BcgI BciVI BclI BlpI BmgBI BmtI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoRV FseI FspAI HpaI KasI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PciI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XbaI XhoI XmaI