################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AgeI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AvrII BamHI BbeI BbrPI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraIII EagI EclXI Eco52I EcoRI EcoRV EcoT22I FbaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 293 TCCGGA T^CCGGA AflIII 910 ACACGT A^C[GA][CT]GT AhdI 1494 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 443 CCTCAGG CC^T[ACGT]AGG Aor51HI 737 AGCGCT AGC^GCT Asp700I 1377 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1494 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 790 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 419 TGCGCA TGC^GCA BbvCI 709 GCTGAGG CCTCAGC(-5/-2) BcgI 858 GCAGGAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 998 GCCTCAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseAI 293 TCCGGA T^CCGGA BspEI 293 TCCGGA T^CCGGA BsrBI 1042 GAGCGG CCGCTC(-3/-3) BstEII 1326 GGTGACC G^GT[ACGT]ACC BstPI 1326 GGTGACC G^GT[ACGT]ACC BstYI 1013 AGATCC [GA]^GATC[CT] Bsu36I 443 CCTCAGG CC^T[ACGT]AGG Eam1105I 1494 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 189 GAAGAG CTCTTC(1/4) Eco47III 737 AGCGCT AGC^GCT Eco81I 443 CCTCAGG CC^T[ACGT]AGG EcoNI 1152 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1326 GGTGACC G^GT[ACGT]ACC EcoT14I 509 CCATGG C^C[AT][AT]GG FseI 1338 GGCCGGCC GGCCGG^CC FspI 419 TGCGCA TGC^GCA HaeII 737 AGCGCT [GA]GCGC^[CT] Ksp632I 189 GAAGAG CTCTTC(1/4) MflI 1013 AGATCC [GA]^GATC[CT] MroI 293 TCCGGA T^CCGGA NaeI 1339 GCCGGC GCC^GGC NcoI 509 CCATGG C^CATGG NgoMIV 1339 GCCGGC G^CCGGC NspI 1175 ACATGC [GA]CATG^[CT] PflFI 790 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1447 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 62 AGTACT AGT^ACT SexAI 791 ACCTGGT A^CC[AT]GGT SfaNI 819 GATGC GCATC(5/9) SnaBI 650 TACGTA TAC^GTA StyI 509 CCATGG C^C[AT][AT]GG Tth111I 790 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1389 CCACACAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1013 AGATCC [GA]^GATC[CT] XmnI 1377 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 438 CGCG CG^CG 787 CGCG AvaI 895 CCCGAG C^[CT]CG[GA]G 1057 CCCGAG BalI 162 TGGCCA TGG^CCA 1278 TGGCCA BanI 621 GGCACC G^G[CT][GA]CC 873 GGTGCC BsaAI 650 TACGTA [CT]AC^GT[GA] 911 CACGTA BsaWI 293 TCCGGA [AT]^CCGG[AT] 1203 ACCGGA BseRI 10 GAGGAG GAGGAG(10/8) 192 GAGGAG BsmAI 80 GAGAC GTCTC(1/5) 1492 GAGAC BsmBI 80 GAGACG CGTCTC(1/5) 1492 GAGACG BsoBI 895 CCCGAG C^[CT]CG[GA]G 1057 CCCGAG BsrFI 644 ACCGGC [GA]^CCGG[CT] 1339 GCCGGC BstUI 438 CGCG CG^CG 787 CGCG BstXI 599 CCATGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1127 CCACGTTTGTGG Cfr10I 644 ACCGGC [GA]^CCGG[CT] 1339 GCCGGC DrdI 85 GACCTCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 844 GACCATTACGTC DsaI 509 CCATGG C^C[GA][CT]GG 1334 CCGTGG HgaI 436 GACGC GACGC(5/10) 1293 GACGC HinfI 681 GACTC G^A[ACGT]TC 730 GAGTC MluNI 162 TGGCCA TGG^CCA 1278 TGGCCA MscI 162 TGGCCA TGG^CCA 1278 TGGCCA MvnI 438 CGCG CG^CG 787 CGCG PleI 681 GACTC GAGTC(4/5) 730 GAGTC PpuMI 131 AGGACCT [GA]G^G[AT]CC[CT] 371 AGGACCT PstI 340 CTGCAG CTGCA^G 1372 CTGCAG PvuII 52 CAGCTG CAG^CTG 1487 CAGCTG SfcI 340 CTGCAG C^T[GA][CT]AG 1372 CTGCAG StuI 200 AGGCCT AGG^CCT 1529 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TaqI TseI Tsp45I TspRI TthHB8I