################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstNI BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MunI MvaI MwoI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI SbfI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 34 CGCG CG^CG AfaI 192 GTAC GT^AC Aor51HI 114 AGCGCT AGC^GCT AvaII 13 GGTCC G^G[AT]CC BbvI 142 GCAGC GCAGC(8/12) BcgI 63 GCACTCCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 117 GCTTAGC GC^T[ACGT]AGC Bpu1102I 117 GCTTAGC GC^T[ACGT]AGC BsmAI 305 GAGAC GTCTC(1/5) BsrI 194 ACTGG ACTGG(1/-1) BstBI 256 TTCGAA TT^CGAA BstUI 34 CGCG CG^CG Cac8I 74 GCCTGC GC[ACGT]^[ACGT]GC CelII 117 GCTTAGC GC^T[ACGT]AGC CfoI 115 GCGC GCG^C DsaI 33 CCGCGG C^C[GA][CT]GG EarI 302 GAAGAG CTCTTC(1/4) Eco47III 114 AGCGCT AGC^GCT FauI 35 GCGGG CCCGC(4/6) HaeII 114 AGCGCT [GA]GCGC^[CT] HhaI 115 GCGC GCG^C HinP1I 115 GCGC G^CGC HinfI 259 GAATC G^A[ACGT]TC HphI 82 GGTGA GGTGA(8/7) Ksp632I 302 GAAGAG CTCTTC(1/4) KspI 33 CCGCGG CCGC^GG MboII 302 GAAGA GAAGA(8/7) MspA1I 33 CCGCGG C[AC]G^C[GT]G MvnI 34 CGCG CG^CG NspV 256 TTCGAA TT^CGAA PpuMI 12 AGGTCCC [GA]G^G[AT]CC[CT] RsaI 192 GTAC GT^AC SacII 33 CCGCGG CCGC^GG ScaI 191 AGTACT AGT^ACT SfaNI 223 GATGC GCATC(5/9) SfcI 110 CTACAG C^T[GA][CT]AG SfuI 256 TTCGAA TT^CGAA SspI 287 AATATT AAT^ATT TaqI 257 TCGA T^CGA TfiI 259 GAATC G^A[AT]TC TseI 142 GCAGC G^C[AT]GC TspRI 277 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 257 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 33 CCGC CCGC(-3/-1) 35 GCGG AcsI 316 AAATTC [GA]^AATT[CT] 328 AAATTT AluI 121 AGCT AG^CT 250 AGCT ApaI 3 GGGCCC GGGCC^C 179 GGGCCC ApoI 316 AAATTC [GA]^AATT[CT] 328 AAATTT BanII 3 GGGCCC G[GA]GC[CT]^C 179 GGGCCC BmyI 3 GGGCCC G[AGT]GC[ACT]^C 179 GGGCCC BsiYI 28 CCGAGCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 272 CCCAACAGTGG BslI 28 CCGAGCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 272 CCCAACAGTGG Bsp1286I 3 GGGCCC G[AGT]GC[ACT]^C 179 GGGCCC BstF5I 186 GGATG GGATG(2/0) 222 GGATG DdeI 118 CTTAG C^T[ACGT]AG 216 CTTAG DraII 12 AGGTCCC [GA]G^G[ACGT]CC[CT] 178 GGGGCCC EcoO109I 12 AGGTCCC [GA]G^G[ACGT]CC[CT] 178 GGGGCCC Fnu4HI 32 GCCGC GC^[ACGT]GC 142 GCAGC FokI 186 GGATG GGATG(9/13) 222 GGATG HapII 7 CCGG C^CGG 183 CCGG HpaII 7 CCGG C^CGG 183 CCGG ItaI 32 GCCGC GC^[ACGT]GC 142 GCAGC MseI 244 TTAA T^TAA 313 TTAA MspI 7 CCGG C^CGG 183 CCGG SmaI 6 CCCGGG CCC^GGG 182 CCCGGG Tru9I 244 TTAA T^TAA 313 TTAA XmaCI 6 CCCGGG C^CCGGG 182 CCCGGG XmaI 6 CCCGGG C^CCGGG 182 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AvaI BcnI BsaJI BsmFI BsoBI BssKI Cfr13I HaeIII MnlI NciI NlaIV Sau96I ScrFI Tsp509I