(Linear) (Six-base) MAPSORT of: KIEE5545.seq Check: 9617 from: 1 to: 1164 KIEE5545.orf With 182 enzymes: SgfI * January 29, 2014 12:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 407 1164 Size: 407 757 AflIII A'CryG_T Cuts at: 0 326 1164 Size: 326 838 AlwNI CAG_nnn'CTG Cuts at: 0 10 597 621 999 1164 Size: 10 587 24 378 165 Fragments arranged by size: 587 378 165 24 10 ApaI G_GGCC'C Cuts at: 0 58 1164 Size: 58 1106 AvaI C'yCGr_G Cuts at: 0 44 130 406 547 905 984 1132 1164 Size: 44 86 276 141 358 79 148 32 Fragments arranged by size: 358 276 148 141 86 79 44 32 BanI G'GyrC_C Cuts at: 0 1041 1164 Size: 1041 123 BanII G_rGCy'C Cuts at: 0 58 130 164 635 1139 1164 Size: 58 72 34 471 504 25 Fragments arranged by size: 504 471 72 58 34 25 BbsI GAAGACnn'nnnn_ Cuts at: 0 660 1164 Size: 660 504 BglI GCCn_nnn'nGGC Cuts at: 0 187 1105 1164 Size: 187 918 59 Fragments arranged by size: 918 187 59 BglII A'GATC_T Cuts at: 0 320 1164 Size: 320 844 BlpI GC'TnA_GC Cuts at: 0 565 781 1164 Size: 565 216 383 Fragments arranged by size: 565 383 216 Bme1580I G_kGCm'C Cuts at: 0 58 895 1046 1164 Size: 58 837 151 118 Fragments arranged by size: 837 151 118 58 BmrI ACTGGGnnnn_n' Cuts at: 0 233 292 1164 Size: 233 59 872 Fragments arranged by size: 872 233 59 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 192 1134 1164 Size: 192 942 30 Fragments arranged by size: 942 192 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 619 1105 1164 Size: 49 570 486 59 Fragments arranged by size: 570 486 59 49 Bpu10I CC'TnA_GC Cuts at: 0 757 1164 Size: 757 407 BsaI GGTCTCn'nnnn_ Cuts at: 0 565 1164 Size: 565 599 BsaAI yAC'GTr Cuts at: 0 561 1164 Size: 561 603 BsaHI Gr'CG_yC Cuts at: 0 254 1164 Size: 254 910 BsaWI w'CCGG_w Cuts at: 0 422 1164 Size: 422 742 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 403 433 1121 1151 1164 Size: 403 30 688 30 13 Fragments arranged by size: 688 403 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 424 427 565 748 832 1164 Size: 424 3 138 183 84 332 Fragments arranged by size: 424 332 183 138 84 3 BseYI C'CCAG_C Cuts at: 0 357 584 915 1164 Size: 357 227 331 249 Fragments arranged by size: 357 331 249 227 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 1164 Size: 385 779 BsiHKAI G_wGCw'C Cuts at: 0 1139 1164 Size: 1139 25 Bsp1286I G_dGCh'C Cuts at: 0 58 130 164 635 895 1046 1139 1164 Size: 58 72 34 471 260 151 93 25 Fragments arranged by size: 471 260 151 93 72 58 34 25 BspHI T'CATG_A Cuts at: 0 314 1164 Size: 314 850 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 407 1164 Size: 407 757 BsrBI CCG'CTC Cuts at: 0 219 777 912 1164 Size: 219 558 135 252 Fragments arranged by size: 558 252 219 135 BsrDI GCAATG_nn' Cuts at: 0 371 1164 Size: 371 793 BssHII G'CGCG_C Cuts at: 0 639 1164 Size: 639 525 BssSI C'ACGA_G Cuts at: 0 484 1065 1164 Size: 484 581 99 Fragments arranged by size: 581 484 99 BstAPI GCAn_nnn'nTGC Cuts at: 0 10 467 1164 Size: 10 457 697 Fragments arranged by size: 697 457 10 BstBI TT'CG_AA Cuts at: 0 698 1164 Size: 698 466 BstXI CCAn_nnnn'nTGG Cuts at: 0 384 1164 Size: 384 780 BstYI r'GATC_y Cuts at: 0 320 1164 Size: 320 844 Bsu36I CC'TnA_GG Cuts at: 0 235 1164 Size: 235 929 BtgI C'CryG_G Cuts at: 0 1100 1164 Size: 1100 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 47 1164 Size: 47 1117 EaeI y'GGCC_r Cuts at: 0 302 1164 Size: 302 862 EcoICRI GAG'CTC Cuts at: 0 1137 1164 Size: 1137 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 619 1105 1164 Size: 49 570 486 59 Fragments arranged by size: 570 486 59 49 EcoNI CCTnn'n_nnAGG Cuts at: 0 238 886 1164 Size: 238 648 278 Fragments arranged by size: 648 278 238 EcoO109I rG'GnC_Cy Cuts at: 0 54 55 135 479 543 945 1164 Size: 54 1 80 344 64 402 219 Fragments arranged by size: 402 344 219 80 64 54 1 FspI TGC'GCA Cuts at: 0 835 1164 Size: 835 329 HaeII r_GCGC'y Cuts at: 0 663 1164 Size: 663 501 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1126 1158 1164 Size: 1126 32 6 Fragments arranged by size: 1126 32 6 MslI CAynn'nnrTG Cuts at: 0 1078 1164 Size: 1078 86 MspA1I CmG'CkG Cuts at: 0 10 122 249 618 825 1102 1164 Size: 10 112 127 369 207 277 62 Fragments arranged by size: 369 277 207 127 112 62 10 PasI CC'CwG_GG Cuts at: 0 139 1164 Size: 139 1025 PflMI CCAn_nnn'nTGG Cuts at: 0 597 1164 Size: 597 567 PfoI T'CCnGG_A Cuts at: 0 938 1164 Size: 938 226 PpuMI rG'GwC_Cy Cuts at: 0 479 543 1164 Size: 479 64 621 Fragments arranged by size: 621 479 64 PspOMI G'GGCC_C Cuts at: 0 54 1164 Size: 54 1110 PstI C_TGCA'G Cuts at: 0 440 890 953 1164 Size: 440 450 63 211 Fragments arranged by size: 450 440 211 63 PvuII CAG'CTG Cuts at: 0 618 1164 Size: 618 546 SacI G_AGCT'C Cuts at: 0 1139 1164 Size: 1139 25 SacII CC_GC'GG Cuts at: 0 1103 1164 Size: 1103 61 SanDI GG'GwC_CC Cuts at: 0 479 1164 Size: 479 685 SbfI CC_TGCA'GG Cuts at: 0 890 1164 Size: 890 274 SexAI A'CCwGG_T Cuts at: 0 83 1164 Size: 83 1081 SfcI C'TryA_G Cuts at: 0 436 886 949 1164 Size: 436 450 63 215 Fragments arranged by size: 450 436 215 63 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1105 1164 Size: 1105 59 SmaI CCC'GGG Cuts at: 0 46 1164 Size: 46 1118 StyI C'CwwG_G Cuts at: 0 578 797 1164 Size: 578 219 367 Fragments arranged by size: 578 367 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 349 1164 Size: 349 815 TatI w'GTAC_w Cuts at: 0 203 839 1164 Size: 203 636 325 Fragments arranged by size: 636 325 203 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 297 1164 Size: 297 867 XmaI C'CCGG_G Cuts at: 0 44 1164 Size: 44 1120 Enzymes that do cut: AarI AflIII AlwNI ApaI AvaI BanI BanII BbsI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI EaeI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MslI MspA1I PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SbfI SexAI SfcI SfiI SmaI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI BpuEI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI ScaI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI