################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AvrII BalI BbeI BbvCI BciVI BfrI BlnI BsaBI BseAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI NarI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflMI PinAI PmeI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AatII 475 GACGTC GACGT^C AccI 931 GTCTAC GT^[AC][GT]AC AcsI 743 AAATTC [GA]^AATT[CT] AfaI 624 GTAC GT^AC AlwNI 1483 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1073 CCTCAGG CC^T[ACGT]AGG ApoI 743 AAATTC [GA]^AATT[CT] AspHI 1036 GAGCTC G[AT]GC[AT]^C AspI 799 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 296 TGCGCA TGC^GCA BamHI 323 GGATCC G^GATCC BanI 559 GGCACC G^G[CT][GA]CC BanII 1036 GAGCTC G[GA]GC[CT]^C BcgI 299 GCACCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1580 CTAG C^TAG BglII 311 AGATCT A^GATCT BlpI 114 GCTCAGC GC^T[ACGT]AGC BpmI 109 CTCCAG CTGGAG(16/14) Bpu1102I 114 GCTCAGC GC^T[ACGT]AGC BsaI 1171 GAGACC GGTCTC(1/5) BsaWI 192 ACCGGA [AT]^CCGG[AT] BseRI 832 GAGGAG GAGGAG(10/8) BsiEI 704 CGGCCG CG[GA][CT]^CG BsiHKAI 1036 GAGCTC G[AT]GC[AT]^C BsmBI 1197 CGTCTC CGTCTC(1/5) BspHI 341 TCATGA T^CATGA BsrFI 1210 GCCGGC [GA]^CCGG[CT] BstBI 137 TTCGAA TT^CGAA BstEII 198 GGTCACC G^GT[ACGT]ACC BstPI 198 GGTCACC G^GT[ACGT]ACC BstXI 503 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1073 CCTCAGG CC^T[ACGT]AGG CelII 114 GCTCAGC GC^T[ACGT]AGC Cfr10I 1210 GCCGGC [GA]^CCGG[CT] DraIII 588 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 704 CGGCCG C^GGCCG EclXI 704 CGGCCG C^GGCCG Eco52I 704 CGGCCG C^GGCCG Eco81I 1073 CCTCAGG CC^T[ACGT]AGG EcoO65I 198 GGTCACC G^GT[ACGT]ACC FspI 296 TGCGCA TGC^GCA HaeII 1062 GGCGCT [GA]GCGC^[CT] MaeI 1580 CTAG C^TAG MseI 221 TTAA T^TAA NaeI 1210 GCCGGC GCC^GGC NcoI 102 CCATGG C^CATGG NgoMIV 1210 GCCGGC G^CCGGC NspV 137 TTCGAA TT^CGAA PflFI 799 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 784 GAGTC GAGTC(4/5) PpuMI 1520 GGGACCC [GA]G^G[AT]CC[CT] PshAI 1233 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 466 CTGCAG CTGCA^G RcaI 341 TCATGA T^CATGA RsaI 624 GTAC GT^AC SacI 1036 GAGCTC GAGCT^C SexAI 1297 ACCTGGT A^CC[AT]GGT SfuI 137 TTCGAA TT^CGAA SmlI 375 CTCAAG C^T[CT][GA]AG StuI 260 AGGCCT AGG^CCT TfiI 1576 GATTC G^A[AT]TC Tru9I 221 TTAA T^TAA Tsp509I 744 AATT ^AATT Tth111I 799 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 254 CGCG CG^CG 1273 CGCG AflIII 291 ACACGT A^C[GA][CT]GT 852 ACACGT Asp700I 597 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1413 GAAGCACTTC BbrPI 292 CACGTG CAC^GTG 853 CACGTG BbsI 478 GTCTTC GAAGAC(2/6) 1000 GTCTTC BclI 237 TGATCA T^GATCA 434 TGATCA BcnI 387 CCCGG CC^[GC]GG 978 CCGGG BglI 531 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 635 GCCTGGTGGGC BpuAI 478 GTCTTC GAAGAC(2/6) 1000 GTCTTC BsaAI 292 CACGTG [CT]AC^GT[GA] 853 CACGTG BsgI 763 CTGCAC GTGCAG(16/14) 1480 GTGCAG BspMI 680 ACCTGC ACCTGC(4/8) 1482 GCAGGT BsrBI 185 CCGCTC CCGCTC(-3/-3) 991 CCGCTC BssSI 155 CACGAG CACGAG(-5/-1) 58 CTCGTG BstUI 254 CGCG CG^CG 1273 CGCG EaeI 704 CGGCCG [CT]^GGCC[GA] 1208 TGGCCG EcoNI 432 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 825 CCTCTTTGAGG FbaI 237 TGATCA T^GATCA 434 TGATCA HinfI 784 GAGTC G^A[ACGT]TC 1576 GATTC MslI 288 CACACACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 748 CACATCAATG MvnI 254 CGCG CG^CG 1273 CGCG NciI 387 CCCGG CC^[GC]GG 978 CCGGG PmaCI 292 CACGTG CAC^GTG 853 CACGTG PmlI 292 CACGTG CAC^GTG 853 CACGTG SfcI 466 CTGCAG C^T[GA][CT]AG 1008 CTACAG XmnI 597 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1413 GAAGCACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcyI AluI AlwI AvaII BbvI BmyI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV SapI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII