(Linear) (Six-base) MAPSORT of: KIEE2579.seq Check: 7897 from: 1 to: 1665 KIEE2579.orf With 182 enzymes: SgfI * June 25, 2015 11:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1473 1665 Size: 1473 192 AatII G_ACGT'C Cuts at: 0 479 1665 Size: 479 1186 AccI GT'mk_AC Cuts at: 0 932 1665 Size: 932 733 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1168 1665 Size: 1168 497 AflIII A'CryG_T Cuts at: 0 291 852 1665 Size: 291 561 813 Fragments arranged by size: 813 561 291 AleI CACnn'nnGTG Cuts at: 0 292 1665 Size: 292 1373 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1527 1665 Size: 1527 138 AlwNI CAG_nnn'CTG Cuts at: 0 1488 1665 Size: 1488 177 ApoI r'AATT_y Cuts at: 0 743 1665 Size: 743 922 BamHI G'GATC_C Cuts at: 0 323 1665 Size: 323 1342 BanI G'GyrC_C Cuts at: 0 559 1665 Size: 559 1106 BanII G_rGCy'C Cuts at: 0 1040 1665 Size: 1040 625 BbsI GAAGACnn'nnnn_ Cuts at: 0 471 993 1665 Size: 471 522 672 Fragments arranged by size: 672 522 471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 288 322 1665 Size: 288 34 1343 Fragments arranged by size: 1343 288 34 BclI T'GATC_A Cuts at: 0 237 434 1665 Size: 237 197 1231 Fragments arranged by size: 1231 237 197 BglI GCCn_nnn'nGGC Cuts at: 0 537 641 1665 Size: 537 104 1024 Fragments arranged by size: 1024 537 104 BglII A'GATC_T Cuts at: 0 311 1665 Size: 311 1354 BlpI GC'TnA_GC Cuts at: 0 115 1665 Size: 115 1550 Bme1580I G_kGCm'C Cuts at: 0 562 589 1665 Size: 562 27 1076 Fragments arranged by size: 1076 562 27 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 1665 Size: 94 1571 Bpu10I CC'TnA_GC Cuts at: 0 886 1665 Size: 886 779 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 360 1665 Size: 360 1305 BsaI GGTCTCn'nnnn_ Cuts at: 0 1165 1665 Size: 1165 500 BsaAI yAC'GTr Cuts at: 0 294 855 1665 Size: 294 561 810 Fragments arranged by size: 810 561 294 BsaHI Gr'CG_yC Cuts at: 0 476 800 1196 1665 Size: 476 324 396 469 Fragments arranged by size: 476 469 396 324 BsaWI w'CCGG_w Cuts at: 0 192 1665 Size: 192 1473 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 171 201 1665 Size: 171 30 1464 Fragments arranged by size: 1464 171 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 847 1665 Size: 847 818 BseYI C'CCAG_C Cuts at: 0 1278 1287 1488 1585 1665 Size: 1278 9 201 97 80 Fragments arranged by size: 1278 201 97 80 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 748 1501 1665 Size: 748 753 164 Fragments arranged by size: 753 748 164 BsiEI CG_ry'CG Cuts at: 0 707 1665 Size: 707 958 BsiHKAI G_wGCw'C Cuts at: 0 1040 1665 Size: 1040 625 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1203 1665 Size: 1203 462 Bsp1286I G_dGCh'C Cuts at: 0 562 589 1040 1665 Size: 562 27 451 625 Fragments arranged by size: 625 562 451 27 BspHI T'CATG_A Cuts at: 0 341 1665 Size: 341 1324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 689 1473 1665 Size: 689 784 192 Fragments arranged by size: 784 689 192 BsrBI CCG'CTC Cuts at: 0 187 993 1665 Size: 187 806 672 Fragments arranged by size: 806 672 187 BsrFI r'CCGG_y Cuts at: 0 1210 1665 Size: 1210 455 BssSI C'ACGA_G Cuts at: 0 58 155 1665 Size: 58 97 1510 Fragments arranged by size: 1510 97 58 BstBI TT'CG_AA Cuts at: 0 138 1665 Size: 138 1527 BstEII G'GTnAC_C Cuts at: 0 198 1665 Size: 198 1467 BstXI CCAn_nnnn'nTGG Cuts at: 0 510 1665 Size: 510 1155 BstYI r'GATC_y Cuts at: 0 41 311 323 1665 Size: 41 270 12 1342 Fragments arranged by size: 1342 270 41 12 Bsu36I CC'TnA_GG Cuts at: 0 1074 1665 Size: 1074 591 BtgI C'CryG_G Cuts at: 0 102 578 707 716 1202 1540 1665 Size: 102 476 129 9 486 338 125 Fragments arranged by size: 486 476 338 129 125 102 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 927 1251 1338 1365 1665 Size: 927 324 87 27 300 Fragments arranged by size: 927 324 300 87 27 BtsI GCAGTG_nn' Cuts at: 0 532 1442 1665 Size: 532 910 223 Fragments arranged by size: 910 532 223 DraIII CAC_nnn'GTG Cuts at: 0 593 1665 Size: 593 1072 EaeI y'GGCC_r Cuts at: 0 704 1208 1665 Size: 704 504 457 Fragments arranged by size: 704 504 457 EagI C'GGCC_G Cuts at: 0 704 1665 Size: 704 961 EarI CTCTTCn'nnn_ Cuts at: 0 493 691 1000 1045 1261 1665 Size: 493 198 309 45 216 404 Fragments arranged by size: 493 404 309 216 198 45 EcoICRI GAG'CTC Cuts at: 0 1038 1665 Size: 1038 627 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 1168 1665 Size: 94 1074 497 Fragments arranged by size: 1074 497 94 EcoNI CCTnn'n_nnAGG Cuts at: 0 436 829 1665 Size: 436 393 836 Fragments arranged by size: 836 436 393 EcoO109I rG'GnC_Cy Cuts at: 0 429 1365 1507 1521 1665 Size: 429 936 142 14 144 Fragments arranged by size: 936 429 144 142 14 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1434 1665 Size: 1434 231 FspI TGC'GCA Cuts at: 0 298 1665 Size: 298 1367 FspAI rTGC'GCAy Cuts at: 0 298 1665 Size: 298 1367 HaeII r_GCGC'y Cuts at: 0 1066 1665 Size: 1066 599 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1364 1396 1665 Size: 1364 32 269 Fragments arranged by size: 1364 269 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 497 1665 Size: 497 1168 MslI CAynn'nnrTG Cuts at: 0 292 752 1665 Size: 292 460 913 Fragments arranged by size: 913 460 292 NaeI GCC'GGC Cuts at: 0 1212 1665 Size: 1212 453 NcoI C'CATG_G Cuts at: 0 102 1665 Size: 102 1563 NgoMIV G'CCGG_C Cuts at: 0 1210 1665 Size: 1210 455 PasI CC'CwG_GG Cuts at: 0 906 1665 Size: 906 759 PfoI T'CCnGG_A Cuts at: 0 386 509 671 1403 1665 Size: 386 123 162 732 262 Fragments arranged by size: 732 386 262 162 123 PmlI CAC'GTG Cuts at: 0 294 855 1665 Size: 294 561 810 Fragments arranged by size: 810 561 294 PpuMI rG'GwC_Cy Cuts at: 0 1521 1665 Size: 1521 144 PshAI GACnn'nnGTC Cuts at: 0 1237 1665 Size: 1237 428 PstI C_TGCA'G Cuts at: 0 470 1665 Size: 470 1195 SacI G_AGCT'C Cuts at: 0 1040 1665 Size: 1040 625 SanDI GG'GwC_CC Cuts at: 0 1521 1665 Size: 1521 144 SapI GCTCTTCn'nnn_ Cuts at: 0 691 1000 1045 1261 1665 Size: 691 309 45 216 404 Fragments arranged by size: 691 404 309 216 45 SexAI A'CCwGG_T Cuts at: 0 1297 1665 Size: 1297 368 SfcI C'TryA_G Cuts at: 0 466 1008 1665 Size: 466 542 657 Fragments arranged by size: 657 542 466 SmlI C'TyrA_G Cuts at: 0 375 1665 Size: 375 1290 StuI AGG'CCT Cuts at: 0 262 1665 Size: 262 1403 StyI C'CwwG_G Cuts at: 0 102 1337 1515 1614 1665 Size: 102 1235 178 99 51 Fragments arranged by size: 1235 178 102 99 51 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 171 203 446 478 1545 1577 1665 Size: 171 32 243 32 1067 32 88 Fragments arranged by size: 1067 243 171 88 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 802 1665 Size: 802 863 XmnI GAAnn'nnTTC Cuts at: 0 601 1417 1665 Size: 601 816 248 Fragments arranged by size: 816 601 248 ZraI GAC'GTC Cuts at: 0 477 1665 Size: 477 1188 Enzymes that do cut: AarI AatII AccI AcuI AflIII AleI AlfI AlwNI ApoI BamHI BanI BanII BbsI BcgI BclI BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I MmeI MslI NaeI NcoI NgoMIV PasI PfoI PmlI PpuMI PshAI PstI SacI SanDI SapI SexAI SfcI SmlI StuI StyI TstI Tth111I XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BciVI BfrBI BmgBI BmrI BmtI BplI BsaBI BsiWI BsmI BspEI BsrDI BsrGI BssHII BstAPI BstZ17I ClaI CspCI DraI DrdI EciI EcoRI EcoRV FseI HincII HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI XbaI XcmI XhoI XmaI