################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## Acc65I AccI AccII AccIII AciI AclI AcsI AfaI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstYI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 709 GACGTC GACGT^C AcyI 709 GACGTC G[GA]^CG[CT]C AflIII 544 ACATGT A^C[GA][CT]GT AhdI 716 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 742 GGATC GGATC(4/5) AspEI 716 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BclI 242 TGATCA T^GATCA BfaI 914 CTAG C^TAG BlpI 64 GCTGAGC GC^T[ACGT]AGC Bpu1102I 64 GCTGAGC GC^T[ACGT]AGC BsaHI 709 GACGTC G[GA]^CG[CT]C BsmAI 539 GAGAC GTCTC(1/5) BsmI 562 GCATTC GAATGC(1/-1) BspLU11I 544 ACATGT A^CATGT BsrFI 763 ACCGGC [GA]^CCGG[CT] BsrI 16 CCAGT ACTGG(1/-1) BstAPI 780 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 816 GGTGACC G^GT[ACGT]ACC BstPI 816 GGTGACC G^GT[ACGT]ACC CelII 64 GCTGAGC GC^T[ACGT]AGC Cfr10I 763 ACCGGC [GA]^CCGG[CT] DsaI 518 CCATGG C^C[GA][CT]GG Eam1105I 716 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 816 GGTGACC G^GT[ACGT]ACC FbaI 242 TGATCA T^GATCA Hin1I 709 GACGTC G[GA]^CG[CT]C MaeI 914 CTAG C^TAG MboII 323 TCTTC GAAGA(8/7) MseI 115 TTAA T^TAA NcoI 518 CCATGG C^CATGG PflMI 513 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfaNI 79 GATGC GCATC(5/9) SmaI 173 CCCGGG CCC^GGG SphI 216 GCATGC GCATG^C Tru9I 115 TTAA T^TAA Van91I 513 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 518 CCATGGCTAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 173 CCCGGG C^CCGGG XmaI 173 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 694 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG 790 CAGGAGCTG AvaI 173 CCCGGG C^[CT]CG[GA]G 619 CCCGAG BalI 103 TGGCCA TGG^CCA 638 TGGCCA BanII 67 GAGCCC G[GA]GC[CT]^C 684 GGGCTC BsoBI 173 CCCGGG C^[CT]CG[GA]G 619 CCCGAG BspMI 408 ACCTGC ACCTGC(4/8) 820 ACCTGC BstXI 338 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 761 CCACCGGCTTGG DpnII 243 GATC ^GATC 743 GATC HphI 816 GGTGA GGTGA(8/7) 758 TCACC MboI 243 GATC ^GATC 743 GATC MluNI 103 TGGCCA TGG^CCA 638 TGGCCA MscI 103 TGGCCA TGG^CCA 638 TGGCCA MslI 217 CATGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 234 CACCTATGTG MspA1I 30 CAGCTG C[AC]G^C[GT]G 634 CAGCTG NdeII 243 GATC ^GATC 743 GATC PleI 10 GACTC GAGTC(4/5) 89 GACTC PvuII 30 CAGCTG CAG^CTG 634 CAGCTG Sau3AI 243 GATC ^GATC 743 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AluI AvaII BanI BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeII MaeIII MnlI MspI MvaI MwoI NciI NlaIII NlaIV NspI PpuMI PstI Sau96I ScrFI SfcI StyI TfiI TseI Tsp45I TspRI