(Circular) (Six-base) MAPSORT of: pFN21AE5350.seq Check: 9352 from: 1 to: 5623 pFN21AE5350 5623 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2718 3433 278 Size: 53 83 186 936 1182 715 2468 Fragments arranged by size: 2468 1182 936 715 186 83 53 AccI GT'mk_AC Cuts at: 1937 2966 1937 Size: 1029 4594 Acc65I G'GTAC_C Cuts at: 2944 2944 Size: 5623 AclI AA'CG_TT Cuts at: 1563 3750 4123 1563 Size: 2187 373 3063 Fragments arranged by size: 3063 2187 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3686 5070 5338 1632 Size: 2054 1384 268 1917 Fragments arranged by size: 2054 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5426 AflIII A'CryG_T Cuts at: 1203 2549 4522 5499 1203 Size: 1346 1973 977 1327 Fragments arranged by size: 1973 1346 1327 977 AgeI A'CCGG_T Cuts at: 4454 4454 Size: 5623 AhdI GACnn_n'nnGTC Cuts at: 2726 4352 2726 Size: 1626 3997 AleI CACnn'nnGTG Cuts at: 1166 2243 1166 Size: 1077 4546 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5623 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2403 2435 2403 Size: 32 5591 AlwNI CAG_nnn'CTG Cuts at: 2704 2800 3366 4938 2704 Size: 96 566 1572 3389 Fragments arranged by size: 3389 1572 566 96 ApaLI G'TGCA_C Cuts at: 3680 4836 3680 Size: 1156 4467 ApoI r'AATT_y Cuts at: 1493 2932 3158 3310 5419 1493 Size: 1439 226 152 2109 1697 Fragments arranged by size: 2109 1697 1439 226 152 AseI AT'TA_AT Cuts at: 160 1032 4177 160 Size: 872 3145 1606 Fragments arranged by size: 3145 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 AvaI C'yCGr_G Cuts at: 1870 1943 2178 2624 2948 1870 Size: 73 235 446 324 4545 Fragments arranged by size: 4545 446 324 235 73 BamHI G'GATC_C Cuts at: 2953 3320 2953 Size: 367 5256 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2272 2303 2888 2944 4399 Size: 325 217 627 485 31 585 56 1455 Cuts at: 4399 618 Size: 1842 Fragments arranged by size: 1842 1455 627 585 485 325 217 56 31 BanII G_rGCy'C Cuts at: 1391 2076 2693 2942 1391 Size: 685 617 249 4072 Fragments arranged by size: 4072 685 617 249 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4652 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3814 3848 1903 Size: 34 1877 34 3678 Fragments arranged by size: 3678 1877 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3515 4725 3515 Size: 1210 4413 BclI T'GATC_A Cuts at: 1548 2247 1548 Size: 699 4924 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4234 136 Size: 107 122 71 631 605 2562 1525 Fragments arranged by size: 2562 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5618 5618 Size: 5623 BlpI GC'TnA_GC Cuts at: 2070 3040 2070 Size: 970 4653 Bme1580I G_kGCm'C Cuts at: 1790 2306 2893 3684 4840 1790 Size: 516 587 791 1156 2573 Fragments arranged by size: 2573 1156 791 587 516 BmgBI CAC'GTC Cuts at: 1318 2782 1318 Size: 1464 4159 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3354 4312 442 Size: 416 53 83 399 24 1937 958 1753 Fragments arranged by size: 1937 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5623 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2004 2114 2381 2621 4283 1239 Size: 765 110 267 240 1662 2579 Fragments arranged by size: 2579 1662 765 267 240 110 Bpu10I CC'TnA_GC Cuts at: 2529 2692 2529 Size: 163 5460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3748 4613 4911 3748 Size: 865 298 4460 Fragments arranged by size: 4460 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4286 882 Size: 617 2787 2219 Fragments arranged by size: 2787 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3414 493 Size: 711 2210 2702 Fragments arranged by size: 2702 2210 711 BsaBI GATnn'nnATC Cuts at: 5617 5617 Size: 5623 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2715 3430 3812 4447 Size: 53 83 186 936 1182 715 382 635 Cuts at: 4447 275 Size: 1451 Fragments arranged by size: 1451 1182 936 715 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4056 4454 4728 4875 5286 4056 Size: 398 274 147 411 4393 Fragments arranged by size: 4393 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2245 2275 2403 2433 1178 Size: 30 165 30 842 30 128 30 4368 Fragments arranged by size: 4368 842 165 128 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2312 2462 2624 1186 Size: 931 195 150 162 4185 Fragments arranged by size: 4185 931 195 162 150 BseYI C'CCAG_C Cuts at: 2743 4826 5322 2743 Size: 2083 496 3044 Fragments arranged by size: 3044 2083 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2266 2517 2804 2813 1163 Size: 573 530 251 287 9 3973 Fragments arranged by size: 3973 573 530 287 251 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3073 3834 4862 1540 Size: 275 186 1072 761 1028 2301 Fragments arranged by size: 2301 1072 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3684 3769 4840 3684 Size: 85 1071 4467 Fragments arranged by size: 4467 1071 85 BsmI GAATG_Cn' Cuts at: 2567 3134 3227 2567 Size: 567 93 4963 Fragments arranged by size: 4963 567 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2306 2693 2893 2942 3684 3769 Size: 399 286 230 387 200 49 742 85 Cuts at: 3769 4840 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 286 230 200 85 49 BspEI T'CCGG_A Cuts at: 5286 5286 Size: 5623 BspHI T'CATG_A Cuts at: 3512 5242 3512 Size: 1730 3893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2422 2834 2978 844 Size: 1578 412 144 3489 Fragments arranged by size: 3489 1578 412 144 BsrBI CCG'CTC Cuts at: 1394 1823 3510 1394 Size: 429 1687 3507 Fragments arranged by size: 3507 1687 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4118 4292 65 Size: 1183 2870 174 1396 Fragments arranged by size: 2870 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2768 4267 4454 1700 Size: 110 958 1499 187 2869 Fragments arranged by size: 2869 1499 958 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5623 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5623 BssSI C'ACGA_G Cuts at: 3683 4695 3683 Size: 1012 4611 BstAPI GCAn_nnn'nTGC Cuts at: 794 2791 794 Size: 1997 3626 BstBI TT'CG_AA Cuts at: 4427 4427 Size: 5623 BstEII G'GTnAC_C Cuts at: 1184 2821 1184 Size: 1637 3986 BstXI CCAn_nnnn'nTGG Cuts at: 2350 2773 2350 Size: 423 5200 BstYI r'GATC_y Cuts at: 1972 2953 3320 3705 3722 5174 5260 5272 5618 Size: 981 367 385 17 1452 86 12 346 Cuts at: 5618 1972 Size: 1977 Fragments arranged by size: 1977 1452 981 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2523 513 Size: 552 939 519 3613 Fragments arranged by size: 3613 939 552 519 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4884 Fragments arranged by size: 4884 683 56 BtsI GCAGTG_nn' Cuts at: 3139 3933 3953 3139 Size: 794 20 4809 Fragments arranged by size: 4809 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5623 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5588 DraI TTT'AAA Cuts at: 2927 2927 Size: 5623 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4630 809 Size: 507 3314 1802 Fragments arranged by size: 3314 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2108 2643 2802 Size: 54 1425 50 275 18 278 535 159 Cuts at: 2802 3070 3959 5588 8 Size: 268 889 1629 43 Fragments arranged by size: 1629 1425 889 535 278 275 268 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3070 1537 Size: 275 1258 4090 Fragments arranged by size: 4090 1258 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3553 1353 Size: 2200 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4210 4584 4730 387 Size: 1064 138 2621 374 146 1280 Fragments arranged by size: 2621 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2004 2114 2381 2621 3686 4283 5070 Size: 393 372 110 267 240 1065 597 787 Cuts at: 5070 5338 1239 Size: 268 1524 Fragments arranged by size: 1524 1065 787 597 393 372 268 267 240 110 EcoO109I rG'GnC_Cy Cuts at: 2115 2354 2862 2115 Size: 239 508 4876 Fragments arranged by size: 4876 508 239 EcoRI G'AATT_C Cuts at: 2932 3310 2932 Size: 378 5245 FspI TGC'GCA Cuts at: 4129 4129 Size: 5623 HaeII r_GCGC'y Cuts at: 1678 4770 1678 Size: 3092 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2175 2207 2712 2744 4264 Size: 32 135 32 603 32 505 32 1520 Cuts at: 4264 4296 4338 4370 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1520 603 505 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2967 3213 669 Size: 988 281 1029 246 3079 Fragments arranged by size: 3079 1029 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5623 HpaI GTT'AAC Cuts at: 3213 3213 Size: 5623 KpnI G_GTAC'C Cuts at: 2948 2948 Size: 5623 MfeI C'AATT_G Cuts at: 3222 3222 Size: 5623 MluI A'CGCG_T Cuts at: 5499 5499 Size: 5623 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4737 4921 1305 Size: 3432 184 2007 Fragments arranged by size: 3432 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2110 2645 10 Size: 54 1425 343 278 535 2988 Fragments arranged by size: 2988 1425 535 343 278 54 MslI CAynn'nnrTG Cuts at: 518 1166 2226 2243 3581 3940 4099 518 Size: 648 1060 17 1338 359 159 2042 Fragments arranged by size: 2042 1338 1060 648 359 159 17 MspA1I CmG'CkG Cuts at: 1606 2037 2641 3039 3716 4864 5109 1606 Size: 431 604 398 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 604 431 398 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2523 513 Size: 552 939 519 3613 Fragments arranged by size: 3613 939 552 519 NdeI CA'TA_TG Cuts at: 387 387 Size: 5623 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5623 NotI GC'GGCC_GC Cuts at: 3070 3070 Size: 5623 NspI r_CATG'y Cuts at: 1132 1229 2225 2553 2741 2981 4526 1132 Size: 97 996 328 188 240 1545 2229 Fragments arranged by size: 2229 1545 996 328 240 188 97 PasI CC'CwG_GG Cuts at: 1348 2350 2857 1348 Size: 1002 507 4114 Fragments arranged by size: 4114 1002 507 PciI A'CATG_T Cuts at: 2549 4522 2549 Size: 1973 3650 PflMI CCAn_nnn'nTGG Cuts at: 1108 2524 1108 Size: 1416 4207 PfoI T'CCnGG_A Cuts at: 2079 2079 Size: 5623 PmeI GTTT'AAAC Cuts at: 2927 2927 Size: 5623 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2403 2435 3692 3724 5205 5237 2403 Size: 32 1257 32 1481 32 2789 Fragments arranged by size: 2789 1481 1257 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2115 2354 2862 2115 Size: 239 508 4876 Fragments arranged by size: 4876 508 239 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5623 PsiI TTA'TAA Cuts at: 3193 3193 Size: 5623 PstI C_TGCA'G Cuts at: 830 2093 2175 2761 2975 830 Size: 1263 82 586 214 3478 Fragments arranged by size: 3478 1263 586 214 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5623 PvuII CAG'CTG Cuts at: 2037 2641 2037 Size: 604 5019 SalI G'TCGA_C Cuts at: 1936 2965 1936 Size: 1029 4594 SanDI GG'GwC_CC Cuts at: 2354 2354 Size: 5623 SbfI CC_TGCA'GG Cuts at: 2975 2975 Size: 5623 ScaI AGT'ACT Cuts at: 3871 5583 3871 Size: 1712 3911 SfcI C'TryA_G Cuts at: 826 1045 2089 2171 2757 2971 4106 4787 4978 Size: 219 1044 82 586 214 1135 681 191 Cuts at: 4978 826 Size: 1471 Fragments arranged by size: 1471 1135 1044 681 586 219 214 191 82 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5623 SmaI CCC'GGG Cuts at: 1872 2180 2950 1872 Size: 308 770 4545 Fragments arranged by size: 4545 770 308 SmlI C'TyrA_G Cuts at: 820 1017 1943 3727 4628 4890 820 Size: 197 926 1784 901 262 1553 Fragments arranged by size: 1784 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3414 493 Size: 2921 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5623 SphI G_CATG'C Cuts at: 1132 2225 2981 1132 Size: 1093 756 3774 Fragments arranged by size: 3774 1093 756 SspI AAT'ATT Cuts at: 5 52 3547 5 Size: 47 3495 2081 Fragments arranged by size: 3495 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5623 StyI C'CwwG_G Cuts at: 513 1065 2004 2111 2523 2646 3062 513 Size: 552 939 107 412 123 416 3074 Fragments arranged by size: 3074 939 552 416 412 123 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3650 3667 3820 4005 4450 325 Size: 1255 2070 17 153 185 445 1498 Fragments arranged by size: 2070 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3869 5581 96 Size: 274 80 33 51 1443 1892 1712 138 Fragments arranged by size: 1892 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2245 2277 5583 5615 2245 Size: 32 3306 32 2253 Fragments arranged by size: 3306 2253 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5623 XbaI T'CTAG_A Cuts at: 2959 2959 Size: 5623 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2530 2530 Size: 5623 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5623 XmaI C'CCGG_G Cuts at: 1870 2178 2948 1870 Size: 308 770 4545 Fragments arranged by size: 4545 770 308 XmnI GAAnn'nnTTC Cuts at: 3752 3752 Size: 5623 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2716 3431 276 Size: 53 83 186 936 1182 715 2468 Fragments arranged by size: 2468 1182 936 715 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI