(Linear) (Six-base) MAPSORT of: KIEE5350.seq Check: 427 from: 1 to: 918 KIEE5350.orf With 182 enzymes: SgfI * January 29, 2014 11:56 .. AatII G_ACGT'C Cuts at: 0 713 918 Size: 713 205 AflIII A'CryG_T Cuts at: 0 544 918 Size: 544 374 AhdI GACnn_n'nnGTC Cuts at: 0 721 918 Size: 721 197 AleI CACnn'nnGTG Cuts at: 0 238 918 Size: 238 680 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 398 430 918 Size: 398 32 488 Fragments arranged by size: 488 398 32 AlwNI CAG_nnn'CTG Cuts at: 0 699 795 918 Size: 699 96 123 Fragments arranged by size: 699 123 96 AvaI C'yCGr_G Cuts at: 0 173 619 918 Size: 173 446 299 Fragments arranged by size: 446 299 173 BanI G'GyrC_C Cuts at: 0 267 298 883 918 Size: 267 31 585 35 Fragments arranged by size: 585 267 35 31 BanII G_rGCy'C Cuts at: 0 71 688 918 Size: 71 617 230 Fragments arranged by size: 617 230 71 BclI T'GATC_A Cuts at: 0 242 918 Size: 242 676 BlpI GC'TnA_GC Cuts at: 0 65 918 Size: 65 853 Bme1580I G_kGCm'C Cuts at: 0 301 888 918 Size: 301 587 30 Fragments arranged by size: 587 301 30 BmgBI CAC'GTC Cuts at: 0 777 918 Size: 777 141 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 376 616 918 Size: 109 267 240 302 Fragments arranged by size: 302 267 240 109 Bpu10I CC'TnA_GC Cuts at: 0 524 687 918 Size: 524 163 231 Fragments arranged by size: 524 231 163 BsaHI Gr'CG_yC Cuts at: 0 710 918 Size: 710 208 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 240 270 398 428 918 Size: 240 30 128 30 490 Fragments arranged by size: 490 240 128 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 112 307 457 619 918 Size: 112 195 150 162 299 Fragments arranged by size: 299 195 162 150 112 BseYI C'CCAG_C Cuts at: 0 738 918 Size: 738 180 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 261 512 799 808 918 Size: 261 251 287 9 110 Fragments arranged by size: 287 261 251 110 9 BsmI GAATG_Cn' Cuts at: 0 562 918 Size: 562 356 Bsp1286I G_dGCh'C Cuts at: 0 71 301 688 888 918 Size: 71 230 387 200 30 Fragments arranged by size: 387 230 200 71 30 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 417 829 918 Size: 417 412 89 Fragments arranged by size: 417 412 89 BsrFI r'CCGG_y Cuts at: 0 763 918 Size: 763 155 BstAPI GCAn_nnn'nTGC Cuts at: 0 786 918 Size: 786 132 BstEII G'GTnAC_C Cuts at: 0 816 918 Size: 816 102 BstXI CCAn_nnnn'nTGG Cuts at: 0 345 768 918 Size: 345 423 150 Fragments arranged by size: 423 345 150 BtgI C'CryG_G Cuts at: 0 518 918 Size: 518 400 EaeI y'GGCC_r Cuts at: 0 103 638 797 918 Size: 103 535 159 121 Fragments arranged by size: 535 159 121 103 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 376 616 918 Size: 109 267 240 302 Fragments arranged by size: 302 267 240 109 EcoO109I rG'GnC_Cy Cuts at: 0 110 349 857 918 Size: 110 239 508 61 Fragments arranged by size: 508 239 110 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 170 202 707 739 918 Size: 170 32 505 32 179 Fragments arranged by size: 505 179 170 32 32 MscI TGG'CCA Cuts at: 0 105 640 918 Size: 105 535 278 Fragments arranged by size: 535 278 105 MslI CAynn'nnrTG Cuts at: 0 221 238 918 Size: 221 17 680 Fragments arranged by size: 680 221 17 MspA1I CmG'CkG Cuts at: 0 32 636 918 Size: 32 604 282 Fragments arranged by size: 604 282 32 NcoI C'CATG_G Cuts at: 0 518 918 Size: 518 400 NspI r_CATG'y Cuts at: 0 220 548 736 918 Size: 220 328 188 182 Fragments arranged by size: 328 220 188 182 PasI CC'CwG_GG Cuts at: 0 345 852 918 Size: 345 507 66 Fragments arranged by size: 507 345 66 PciI A'CATG_T Cuts at: 0 544 918 Size: 544 374 PflMI CCAn_nnn'nTGG Cuts at: 0 519 918 Size: 519 399 PfoI T'CCnGG_A Cuts at: 0 74 918 Size: 74 844 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 398 430 918 Size: 398 32 488 Fragments arranged by size: 488 398 32 PpuMI rG'GwC_Cy Cuts at: 0 110 349 857 918 Size: 110 239 508 61 Fragments arranged by size: 508 239 110 61 PstI C_TGCA'G Cuts at: 0 88 170 756 918 Size: 88 82 586 162 Fragments arranged by size: 586 162 88 82 PvuII CAG'CTG Cuts at: 0 32 636 918 Size: 32 604 282 Fragments arranged by size: 604 282 32 SanDI GG'GwC_CC Cuts at: 0 349 918 Size: 349 569 SfcI C'TryA_G Cuts at: 0 84 166 752 918 Size: 84 82 586 166 Fragments arranged by size: 586 166 84 82 SmaI CCC'GGG Cuts at: 0 175 918 Size: 175 743 SphI G_CATG'C Cuts at: 0 220 918 Size: 220 698 StyI C'CwwG_G Cuts at: 0 106 518 641 918 Size: 106 412 123 277 Fragments arranged by size: 412 277 123 106 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 240 272 918 Size: 240 32 646 Fragments arranged by size: 646 240 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 525 918 Size: 525 393 XmaI C'CCGG_G Cuts at: 0 173 918 Size: 173 745 ZraI GAC'GTC Cuts at: 0 711 918 Size: 711 207 Enzymes that do cut: AatII AflIII AhdI AleI AloI AlwNI AvaI BanI BanII BclI BlpI Bme1580I BmgBI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspMI BsrFI BstAPI BstEII BstXI BtgI EaeI Eco57MI EcoO109I Hin4I MscI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PpiI PpuMI PstI PvuII SanDI SfcI SmaI SphI StyI TstI XcmI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AfeI AflII AgeI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmnI