(Circular) (Six-base) MAPSORT of: pFN21AE2519.seq Check: 2000 from: 1 to: 6370 pFN21AE2519 6370 bp With 182 enzymes: SgfI * April 4, 2014 16:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2654 2654 Size: 6370 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4180 278 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3537 3713 1937 Size: 1600 176 4594 Fragments arranged by size: 4594 1600 176 Acc65I G'GTAC_C Cuts at: 3691 3691 Size: 6370 AclI AA'CG_TT Cuts at: 1563 4497 4870 1563 Size: 2934 373 3063 Fragments arranged by size: 3063 2934 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3160 3347 3383 3494 3537 4433 5817 6085 Size: 1528 187 36 111 43 896 1384 268 Cuts at: 6085 1632 Size: 1917 Fragments arranged by size: 1917 1528 1384 896 268 187 111 43 36 AfeI AGC'GCT Cuts at: 2435 3272 2435 Size: 837 5533 AflII C'TTAA_G Cuts at: 820 1017 3659 820 Size: 197 2642 3531 Fragments arranged by size: 3531 2642 197 AflIII A'CryG_T Cuts at: 1203 2211 5269 6246 1203 Size: 1008 3058 977 1327 Fragments arranged by size: 3058 1327 1008 977 AgeI A'CCGG_T Cuts at: 5201 5201 Size: 6370 AhdI GACnn_n'nnGTC Cuts at: 5099 5099 Size: 6370 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6370 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2805 1184 Size: 1621 4749 AlwNI CAG_nnn'CTG Cuts at: 3212 4113 5685 3212 Size: 901 1572 3897 Fragments arranged by size: 3897 1572 901 ApaLI G'TGCA_C Cuts at: 3251 4427 5583 3251 Size: 1176 1156 4038 Fragments arranged by size: 4038 1176 1156 ApoI r'AATT_y Cuts at: 1493 2195 3645 3679 3905 4057 6166 1493 Size: 702 1450 34 226 152 2109 1697 Fragments arranged by size: 2109 1697 1450 702 226 152 34 AseI AT'TA_AT Cuts at: 160 1032 4924 160 Size: 872 3892 1606 Fragments arranged by size: 3892 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 AvaI C'yCGr_G Cuts at: 1870 1943 3695 1870 Size: 73 1752 4545 Fragments arranged by size: 4545 1752 73 BamHI G'GATC_C Cuts at: 3700 4067 3700 Size: 367 6003 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2419 3691 5146 618 Size: 325 217 627 632 1272 1455 1842 Fragments arranged by size: 1842 1455 1272 632 627 325 217 BanII G_rGCy'C Cuts at: 1391 2573 3689 1391 Size: 1182 1116 4072 Fragments arranged by size: 4072 1182 1116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2713 3467 928 Size: 971 814 754 3831 Fragments arranged by size: 3831 971 814 754 BbvCI CC'TCA_GC Cuts at: 3482 3482 Size: 6370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3273 3307 3589 3623 4561 4595 1903 Size: 34 1336 34 282 34 938 34 3678 Fragments arranged by size: 3678 1336 938 282 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4262 5472 4262 Size: 1210 5160 BclI T'GATC_A Cuts at: 1548 1548 Size: 6370 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3293 4981 136 Size: 107 122 71 631 605 1621 1688 1525 Fragments arranged by size: 1688 1621 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3054 6365 3054 Size: 3311 3059 BlpI GC'TnA_GC Cuts at: 3787 3787 Size: 6370 Bme1580I G_kGCm'C Cuts at: 1790 3017 3131 3255 4431 5587 1790 Size: 1227 114 124 1176 1156 2573 Fragments arranged by size: 2573 1227 1176 1156 124 114 BmgBI CAC'GTC Cuts at: 1318 2812 3607 1318 Size: 1494 795 4081 Fragments arranged by size: 4081 1494 795 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2112 2735 4101 Size: 416 53 83 399 24 695 623 1366 Cuts at: 4101 5059 442 Size: 958 1753 Fragments arranged by size: 1753 1366 958 695 623 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6370 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2353 2353 Size: 6370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2080 2348 3121 3239 3244 3297 5030 1239 Size: 841 268 773 118 5 53 1733 2579 Fragments arranged by size: 2579 1733 841 773 268 118 53 5 Bpu10I CC'TnA_GC Cuts at: 3482 3482 Size: 6370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2062 2228 4495 5360 5658 2062 Size: 166 2267 865 298 2774 Fragments arranged by size: 2774 2267 865 298 166 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5033 882 Size: 617 3534 2219 Fragments arranged by size: 3534 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4161 493 Size: 711 2957 2702 Fragments arranged by size: 2957 2702 711 BsaBI GATnn'nnATC Cuts at: 3053 6364 3053 Size: 3311 3059 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3150 3224 4177 4559 Size: 53 83 186 936 1617 74 953 382 Cuts at: 4559 5194 275 Size: 635 1451 Fragments arranged by size: 1617 1451 953 936 635 382 186 83 74 53 BsaWI w'CCGG_w Cuts at: 3111 4803 5201 5475 5622 6033 3111 Size: 1692 398 274 147 411 3448 Fragments arranged by size: 3448 1692 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6145 Fragments arranged by size: 6145 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2870 3368 3449 1186 Size: 1684 498 81 4107 Fragments arranged by size: 4107 1684 498 81 BseYI C'CCAG_C Cuts at: 2318 2537 2659 3302 5573 6069 2318 Size: 219 122 643 2271 496 2619 Fragments arranged by size: 2619 2271 643 496 219 122 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5797 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3820 4581 5609 1540 Size: 275 186 1819 761 1028 2301 Fragments arranged by size: 2301 1819 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2850 2939 3255 4431 4516 5587 2850 Size: 89 316 1176 85 1071 3633 Fragments arranged by size: 3633 1176 1071 316 89 85 BsmI GAATG_Cn' Cuts at: 2409 2773 3493 3881 3974 2409 Size: 364 720 388 93 4805 Fragments arranged by size: 4805 720 388 364 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2147 3182 3215 2147 Size: 1035 33 5302 Fragments arranged by size: 5302 1035 33 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2573 2850 2939 3017 3131 3255 3689 Size: 399 783 277 89 78 114 124 434 Cuts at: 3689 4431 4516 5587 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 434 399 277 124 114 89 85 78 BspEI T'CCGG_A Cuts at: 3111 6033 3111 Size: 2922 3448 BspHI T'CATG_A Cuts at: 2190 4259 5989 2190 Size: 2069 1730 2571 Fragments arranged by size: 2571 2069 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2654 2991 3344 3725 844 Size: 1810 337 353 381 3489 Fragments arranged by size: 3489 1810 381 353 337 BsrBI CCG'CTC Cuts at: 1394 1823 3100 4257 1394 Size: 429 1277 1157 3507 Fragments arranged by size: 3507 1277 1157 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3190 4865 5039 65 Size: 1183 1942 1675 174 1396 Fragments arranged by size: 1942 1675 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2333 5014 5201 1700 Size: 110 523 2681 187 2869 Fragments arranged by size: 2869 2681 523 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6370 BssSI C'ACGA_G Cuts at: 4430 5442 4430 Size: 1012 5358 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6370 BstBI TT'CG_AA Cuts at: 5174 5174 Size: 6370 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6370 BstXI CCAn_nnnn'nTGG Cuts at: 2326 2326 Size: 6370 BstYI r'GATC_y Cuts at: 1972 3054 3700 4067 4452 4469 5921 6007 6019 Size: 1082 646 367 385 17 1452 86 12 Cuts at: 6019 6365 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1082 646 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2137 2610 513 Size: 552 1072 473 4273 Fragments arranged by size: 4273 1072 552 473 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3105 487 Size: 683 56 1879 3752 Fragments arranged by size: 3752 1879 683 56 BtsI GCAGTG_nn' Cuts at: 2787 3886 4680 4700 2787 Size: 1099 794 20 4457 Fragments arranged by size: 4457 1099 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6335 DraI TTT'AAA Cuts at: 3674 3674 Size: 6370 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5377 809 Size: 507 4061 1802 Fragments arranged by size: 4061 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3203 3817 4706 Size: 54 1425 50 275 18 1373 614 889 Cuts at: 4706 6335 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1373 889 614 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3817 1537 Size: 275 2005 4090 Fragments arranged by size: 4090 2005 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2489 2895 3513 4300 1353 Size: 1136 406 618 787 3423 Fragments arranged by size: 3423 1136 787 618 406 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4957 5331 5477 387 Size: 1064 138 3368 374 146 1280 Fragments arranged by size: 3368 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2348 3121 3160 3239 3244 3297 Size: 393 448 268 773 39 79 5 53 Cuts at: 3297 3347 3383 3494 3537 4433 5030 5817 6085 Size: 50 36 111 43 896 597 787 268 Cuts at: 6085 1239 Size: 1524 Fragments arranged by size: 1524 896 787 773 597 448 393 268 268 111 79 53 50 43 39 36 5 EcoO109I rG'GnC_Cy Cuts at: 2069 2274 2315 2737 3299 2069 Size: 205 41 422 562 5140 Fragments arranged by size: 5140 562 422 205 41 EcoRI G'AATT_C Cuts at: 3679 4057 3679 Size: 378 5992 EcoRV GAT'ATC Cuts at: 2494 2494 Size: 6370 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3413 3413 Size: 6370 FspI TGC'GCA Cuts at: 4876 4876 Size: 6370 HaeII r_GCGC'y Cuts at: 1678 2437 3025 3274 5517 1678 Size: 759 588 249 2243 2531 Fragments arranged by size: 2531 2243 759 588 249 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 2369 2401 2933 Size: 32 135 32 626 32 139 32 532 Cuts at: 2933 2965 5011 5043 5085 5117 1373 Size: 32 2046 32 42 32 2626 Fragments arranged by size: 2626 2046 626 532 139 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3568 3714 3960 669 Size: 988 281 1630 146 246 3079 Fragments arranged by size: 3079 1630 988 281 246 146 HindIII A'AGCT_T Cuts at: 748 748 Size: 6370 HpaI GTT'AAC Cuts at: 3960 3960 Size: 6370 KpnI G_GTAC'C Cuts at: 3695 3695 Size: 6370 MfeI C'AATT_G Cuts at: 3969 3969 Size: 6370 MluI A'CGCG_T Cuts at: 6246 6246 Size: 6370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2715 3336 5484 5668 1305 Size: 1410 621 2148 184 2007 Fragments arranged by size: 2148 2007 1410 621 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4548 Fragments arranged by size: 4548 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2369 2810 4328 4687 4846 518 Size: 648 1203 441 1518 359 159 2042 Fragments arranged by size: 2042 1518 1203 648 441 359 159 MspA1I CmG'CkG Cuts at: 1606 2752 2800 3786 4463 5611 5856 1606 Size: 1146 48 986 677 1148 245 2120 Fragments arranged by size: 2120 1148 1146 986 677 245 48 NaeI GCC'GGC Cuts at: 2335 2335 Size: 6370 NcoI C'CATG_G Cuts at: 513 1065 2137 2610 513 Size: 552 1072 473 4273 Fragments arranged by size: 4273 1072 552 473 NdeI CA'TA_TG Cuts at: 387 387 Size: 6370 NgoMIV G'CCGG_C Cuts at: 2333 2333 Size: 6370 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6370 NotI GC'GGCC_GC Cuts at: 3817 3817 Size: 6370 NspI r_CATG'y Cuts at: 1132 1229 3728 5273 1132 Size: 97 2499 1545 2229 Fragments arranged by size: 2499 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2566 1348 Size: 1218 5152 PciI A'CATG_T Cuts at: 5269 5269 Size: 6370 PflMI CCAn_nnn'nTGG Cuts at: 1108 2143 2666 1108 Size: 1035 523 4812 Fragments arranged by size: 4812 1035 523 PmeI GTTT'AAAC Cuts at: 3674 3674 Size: 6370 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4439 4471 5952 5984 4439 Size: 32 1481 32 4825 Fragments arranged by size: 4825 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2069 2737 3299 2069 Size: 668 562 5140 Fragments arranged by size: 5140 668 562 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6370 PsiI TTA'TAA Cuts at: 3940 3940 Size: 6370 PstI C_TGCA'G Cuts at: 830 2314 2988 3270 3722 830 Size: 1484 674 282 452 3478 Fragments arranged by size: 3478 1484 674 452 282 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6370 PvuII CAG'CTG Cuts at: 2752 2752 Size: 6370 SalI G'TCGA_C Cuts at: 1936 3712 1936 Size: 1776 4594 SanDI GG'GwC_CC Cuts at: 2069 2069 Size: 6370 SapI GCTCTTCn'nnn_ Cuts at: 2489 2489 Size: 6370 SbfI CC_TGCA'GG Cuts at: 2314 3722 2314 Size: 1408 4962 ScaI AGT'ACT Cuts at: 4618 6330 4618 Size: 1712 4658 SfcI C'TryA_G Cuts at: 826 1045 2310 2984 3266 3718 4853 5534 5725 Size: 219 1265 674 282 452 1135 681 191 Cuts at: 5725 826 Size: 1471 Fragments arranged by size: 1471 1265 1135 681 674 452 282 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6370 SmaI CCC'GGG Cuts at: 1872 3697 1872 Size: 1825 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2041 2243 3659 4474 5375 5637 Size: 197 926 98 202 1416 815 901 262 Cuts at: 5637 820 Size: 1553 Fragments arranged by size: 1553 1416 926 901 815 262 202 197 98 SnaBI TAC'GTA Cuts at: 493 4161 493 Size: 3668 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6370 SphI G_CATG'C Cuts at: 1132 3728 1132 Size: 2596 3774 SspI AAT'ATT Cuts at: 5 52 4294 5 Size: 47 4242 2081 Fragments arranged by size: 4242 2081 47 StuI AGG'CCT Cuts at: 1517 2676 1517 Size: 1159 5211 StyI C'CwwG_G Cuts at: 513 1065 2073 2119 2137 2610 2907 3384 3809 Size: 552 1008 46 18 473 297 477 425 Cuts at: 3809 513 Size: 3074 Fragments arranged by size: 3074 1008 552 477 473 425 297 46 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2623 4397 4414 4567 4752 5197 325 Size: 1255 1043 1774 17 153 185 445 1498 Fragments arranged by size: 1774 1498 1255 1043 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4616 6328 96 Size: 274 80 33 51 1443 2639 1712 138 Fragments arranged by size: 2639 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2759 2791 6330 6362 2759 Size: 32 3539 32 2767 Fragments arranged by size: 3539 2767 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6370 XbaI T'CTAG_A Cuts at: 3706 3706 Size: 6370 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6370 XmaI C'CCGG_G Cuts at: 1870 3695 1870 Size: 1825 4545 XmnI GAAnn'nnTTC Cuts at: 3107 4499 3107 Size: 1392 4978 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4178 276 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI